syk inhibitor r406  (MedChemExpress)

Journal: Scientific Reports

Article Title: Enhancement of melanoma aggressiveness via p38-MAPK, HIF-1α pathways, and metabolic reprogramming induced by Candida albicans

doi: 10.1038/s41598-025-24055-y

Figure Lengend Snippet: Influence of the viability of Candida albicans and the main pathogen recognition receptors (PRRs) in melanoma cells after six hours of stimulation. ( a ) VEGF release and ( b ) Fos gene expression induced by different morphologies and released molecules of C. albicans . ( c , d ) VEGF release and ( e ) c-Fos protein expression in live C. albicans -exposed melanoma cells alone or treated with inhibitors for c , e TLRs (MyD88 inhibitor: TJ-M2010-5, 30 µM) and CLRs (Syk inhibitor: Piceatannol, 30 µM), and ( d , e ) EGFR ( PD153035 , 1 µM) and EphA2 (NVP-BHG712, 1 µM). ( a – d ) Data were presented as the individual values, means ± SEM ( n = 3 biologically independent samples). Two-tailed unpaired Student’s t test was used to examine the statistical significance between groups. * and ** denote P < 0.05 and P < 0.01 in comparison to control without C. albicans , respectively. ( e ) Representative data of three independent Western Blots. HK: Heat-Killed; CM: Conditioned Media obtained after six hours of exposure of melanoma cells to live C. albicans MOI 5.

Article Snippet: The inhibitors of hexokinase (2-deoxyglucose, 2-DG, Cat # HY-1396; 5 mM), GLUT1 (BAY 876, Cat # HY-100017; 25 nM), SYK (Piceatannol, Cat # HY-13518; 30 μM), MyD88 (TJ-M2010-5, Cat # HY-139397; 30 μM), EGFR ( PD153035 , Cat # HY-14346; 1 μM), EphA2 (NVP-BHG712, Cat # HY-13258 A; 1 μM), ERK1/2 (PD-0325901, Cat # HY-10254; 1 μM), JNK (SP600125, Cat # HY-12041; 10 μM), p38a (SB 203580, Cat # HY-10256; 10 μM), NF-κB (BAY 11-7082, Cat # HY-13453; 2 μM), c-Fos/AP-1 (T-5224, Cat # HY-12270; 10 μM) and HIF-1α (LW6, Cat # HY-13671; 10 μM) were obtained from MedChemExpress.

Techniques: Gene Expression, Expressing, Two Tailed Test, Comparison, Control, Western Blot