human bladder cancer cell lines sw780  (ATCC)

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: SW780 bladder cancer cells produce floating microbladder-like vesicles (luminal cavities characteristic of urothelial differentiation) in monolayer culture. A. Phase-contrast microscopy of T24 and SW780 bladder cancer cells. B. Scanning electron microscopy (SEM) images showing cell surface morphology. Red arrows indicate floating cyst-like vesicles (microbladder-like, luminal cavities characteristic of urothelial differentiation) produced by the SW780 cell line. Images are representative of the overall cell population.

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Microscopy, Electron Microscopy, Produced

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Encapsulation of pheophorbide a enhances PDT efficacy in 3D tumor spheroids. A. Scanning electron microscopy (SEM) images showing the surface morphology of T24 and SW780 spheroids. B. General appearance of bladder tumor spheroids before treatment. Red arrows indicate detached cyst-like vesicles, i.e. “microbladders” (luminal cavities characteristic of urothelial differentiation); dotted circles highlight internal cyst-like structures embedded within the spheroids. C. Counterstained semi-thin transverse sections (500 nm) of spheroids revealing internal cyst-like structures (dotted circles). D. Two-photon microscopy imaging of pheophorbide a (Pheo) (1 µM) penetration in T24 spheroids after 30 min incubation at 37 °C either in its free or encapsulated formulation. Poly (ethylene oxide)-block-poly (ε-caprolactone) (PEO 5000 -PCL 4000 ) empty micelles; Pheophorbide encapsulated in PEO-PCL micelles (Pheo-PEOPCL). Cyan: nuclei (Hoechst); red: pheophorbide fluorescence. E. Spheroid viability assessed by intracellular ATP quantification at 3- and 6-days post-PDT ([Pheo] = 3 µM). Results include data from 1 to 6 independent experiments (N), with a cumulative number of biological replicates (n) ranging from 6 to 39.

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Encapsulation, Electron Microscopy, Microscopy, Imaging, Incubation, Formulation, Blocking Assay, Fluorescence

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Encapsulation of pheophorbide a in PEO-PCL micelles enhances PDT-induced cytotoxicity in 2D high-grade (T24) and low-grade (SW780) bladder cancer cell cultures. A. Cells were treated with empty poly (ethylene oxide)-block-poly (ε-caprolactone) PEO-PCL micelles (PEOPCL) at polymer concentrations equivalent to those used in pheo-loaded micelles, based on the 1:30 drug-to-polymer weight ratio. B. Cells were exposed to increasing concentrations of free pheophorbide a (Pheo) (nM). C. Cells were treated with increasing concentrations of Pheo encapsulated in PEO-PCL micelles (pheo–PEOPCL) (nM). D. Half-maximal inhibitory concentration (IC₅₀) determination for the Pheo–PEOPCL condition. Cell confluence was monitored by videomicroscopy for 72 h post-PDT ( N > 1; n = 6).

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Encapsulation, Blocking Assay, Polymer, Concentration Assay

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Reduced wound closure in bladder cancer cell monolayers following photodynamic treatment with encapsulated pheophorbide a . A. Kinetics of wound closure over 24 h post-PDT in T24 (grade 3) and SW780 (grade 1) monolayers, monitored by videomicroscopy. Cells were treated with increasing concentrations of pheophorbide a encapsulated in poly (ethylene oxide)-block-poly (ε-caprolactone) PEO-PCL micelles (Pheo-PEO-PCL) (nM). B. Quantification of wound closure ( %) at 24 h post-PDT across the different Pheo-PEO-PCL concentrations. Relative wound density ( %) reflects the proportion of the scratch area repopulated by migrating cells. Data are expressed as mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test versus control (0 nM).

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Blocking Assay, Control

Journal: Translational Oncology

Article Title: Improving photodynamic therapy efficacy in bladder cancer using polymer micelle-encapsulated pheophorbide a

doi: 10.1016/j.tranon.2026.102687

Figure Lengend Snippet: Proof of concept of photodynamic therapy efficacy in a complex 3D engineered bladder tumor model. A. Histological cross-sections of the vesical reconstructed tissues stained with Masson’s trichrome, 72 hours after PDT with 3 µM of either free pheophorbide a (Pheo) or pheophorbide a encapsulated in poly (ethylene oxide)-block-poly (ε-caprolactone) PEO-PCL micelles (Pheo–PEO-PCL). Cells appear in red, and stromal collagens in blue. Tumor cells are outlined with black dotted lines. Representative images are shown. B. Tissue viability assessed using the PrestoBlue assay at 48- and 72-hours post-PDT on engineered bladder substitutes implanted with T24 or SW780 spheroids. Results are expressed as a percentage of the untreated control at each timepoint ± SEM.

Article Snippet: Human BC cell lines T24 (ATCC® HTB-4TM, derived from a grade 3 transitional cell carcinoma of an 81-year-old woman) and SW780 (ATCC® CRL-2169TM, grade 1 transitional cell carcinoma from an 80-year-old woman) were purchased in 2022 from ATCC and cultured in Dulbecco’s Modified Eagles Medium containing GlutaMAX supplemented with 10 % of heat-inactivated fetal calf serum, 100 U.mL -1 of penicillin, and 100 μg.mL -1 of streptomycin at 37 °C in a humidified atmosphere at 5 % CO 2 (Panasonic, MCO-170AiCUVH).

Techniques: Staining, Blocking Assay, Prestoblue Assay, Control

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Type I-interferon β induces a strong anti-tumour response in bladder cancer cells

doi: 10.1007/s00432-025-06409-1

Figure Lengend Snippet: Relative mRNA expression of the receptor subunits IFNAR1, IFNAR2, IFNLR1 and IL10RB in SW780 and RT4 cells. RNA was isolated from the individual cell lines, reverse-transcribed into cDNA and then analysed for expression of the IFN RS by qPCR. The expression levels of the individual RS were normalised to the geometric mean of the reference genes TBP and HPRT1

Article Snippet: SW780 cells (ATCC, CRL-2169) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) complete with 4.5 g/l glucose and 10% fetal bovine serum (FBS, all Thermo Fisher Scientific).

Techniques: Expressing, Isolation, Reverse Transcription

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Type I-interferon β induces a strong anti-tumour response in bladder cancer cells

doi: 10.1007/s00432-025-06409-1

Figure Lengend Snippet: Influence of stimulation with 2.5–250,000 pg/ml IFN-α2, -β and -λ1 over 24 h on the cell viability of the RT4 and SW790 cell lines. The viability measurements were performed for 60 min for the RT4 cells and 120 min after addition of WST-1 for the SW780 cells. The respective MW of the measurements after stimulation were related to the negative control to calculate the percentage change. The significance was then calculated using the Mann–Whitney-U test,p > 0.05 (n = 9, 3 trials with triplicates), ± SD

Article Snippet: SW780 cells (ATCC, CRL-2169) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) complete with 4.5 g/l glucose and 10% fetal bovine serum (FBS, all Thermo Fisher Scientific).

Techniques: Negative Control, MANN-WHITNEY

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Type I-interferon β induces a strong anti-tumour response in bladder cancer cells

doi: 10.1007/s00432-025-06409-1

Figure Lengend Snippet: Caspase-3/7 assay for the detection of apoptosis by stimulation of SW780 cells with 25,000 pg/ml IFN-α2, -β, -λ1 and cycloheximide for 24 h and 72 h. Green Object Count / Red Object Count [%] to calculate the proportion of apoptotic cells (green) to cell nuclei (red), ± SD. Cells stimulated with IFN and cycloheximide were compared with non-stimulated cells (n. c.). **: significant, ns: not significant

Article Snippet: SW780 cells (ATCC, CRL-2169) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) complete with 4.5 g/l glucose and 10% fetal bovine serum (FBS, all Thermo Fisher Scientific).

Techniques:

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Type I-interferon β induces a strong anti-tumour response in bladder cancer cells

doi: 10.1007/s00432-025-06409-1

Figure Lengend Snippet: Heatmap of n-fold expression of the ISGs IFIT1, IFIT2, IRF9, ISG15, MX1 and IFI44 after 4 h and 24 h in the BLCA cell lines SW780 and RT4. The cells were stimulated with 2.5–250,000 pg/ml IFN-α2, -β and -λ1. The stimulated samples were normalised to unstimulated samples ( n = 3). RNA was isolated from the individual cell lines, transcribed into cDNA and then expression analysis was performed by qPCR. The expression levels of the individual ISGs were normalised to the geometric mean of the reference genes TBP and HPRT1. The mean value was then calculated from n = 3

Article Snippet: SW780 cells (ATCC, CRL-2169) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) complete with 4.5 g/l glucose and 10% fetal bovine serum (FBS, all Thermo Fisher Scientific).

Techniques: Expressing, Isolation

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Type I-interferon β induces a strong anti-tumour response in bladder cancer cells

doi: 10.1007/s00432-025-06409-1

Figure Lengend Snippet: Example western blot analysis of STAT1 and pSTAT1 in SW780 and RT4 cells after 24 h with calculation of the relative phosphoprotein concentration. Additional western blot analysis of IRF9 was done after 24 h. SW780 and RT4 cells were harvested after 24 h stimulation with 25,000 pg/ml IFN-α2, -β and -λ1, lysed and Western blot labeled with antibodies against a STAT1, b pSTAT1, c IRF9 and (a, b, c) histone H3 and detected via HRP-coupled secondary antibodies. The protein bands were analysed densitometrically and the pSTAT1 and STAT1 protein fraction was normalised to the histone H3 fraction. A ratio was then formed from the normalised pSTAT1 and STAT1 phosphoprotein content to calculate the relative protein content: d (pSTAT1/Histone H3) / (STAT1/Histone H3)

Article Snippet: SW780 cells (ATCC, CRL-2169) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) complete with 4.5 g/l glucose and 10% fetal bovine serum (FBS, all Thermo Fisher Scientific).

Techniques: Western Blot, Concentration Assay, Labeling

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Type I-interferon β induces a strong anti-tumour response in bladder cancer cells

doi: 10.1007/s00432-025-06409-1

Figure Lengend Snippet: Luciferase reporter assay to measure the activation of the IFN-sensitive response element (ISRE). Luciferase activity was measured in the SW780 cell line after 24 h stimulation with 25,000 pg/ml IFN-α2, -β and-λ1 ( n = 4, SD). Relative luciferase activity was calculated by normalising NanoLuc® luciferase activity to Firefly luciferase activity. The pEGFP vector served only as a control for transfection efficiency, but did not induce ISRE

Article Snippet: SW780 cells (ATCC, CRL-2169) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) complete with 4.5 g/l glucose and 10% fetal bovine serum (FBS, all Thermo Fisher Scientific).

Techniques: Luciferase, Reporter Assay, Activation Assay, Activity Assay, Plasmid Preparation, Control, Transfection