Journal: International Journal of Surgery (London, England)
Article Title: Comprehensive analysis of an ATF/CREB family-based signature with regard to prognosis and immune feature in adrenocortical carcinoma: a cohort study
doi: 10.1097/JS9.0000000000002961
Figure Lengend Snippet: Flow diagram of patient inclusion, signature construction, and validation in ACC cohorts. A schematic overview of the study design including sample screening, inclusion, and analytical stages. Adrenocortical carcinoma (ACC) patients from three independent cohorts – The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and West China Hospital (WCH) – were assessed. After applying exclusion criteria, final cohorts included TCGA ( n = 79), GEO ( n = 24), and WCH ( n = 78). A transcription factor-based prognostic signature involving ATF/CREB family genes was constructed using Cox and LASSO Cox regression in the TCGA training cohort. The model’s prognostic and immunological significance was analyzed. External validation was performed in GEO ( GSE10927 ) and WCH cohorts, with further functional assays in SW13 cell line.
Article Snippet: The human ACC cell line SW13 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: Biomarker Discovery, Gene Expression, Construct, Functional Assay