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surescripttmfirst strand cdna synthesis kit  (Genecopoeia)


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    Genecopoeia surescripttmfirst strand cdna synthesis kit
    Surescripttmfirst Strand Cdna Synthesis Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surescript-first-strand-cdna-synthesis-kit/SureScript+First-Strand+cDNA+Synthesis+Kit/pm42044732-93-23-29
    Average 96 stars, based on 270 article reviews
    surescripttmfirst strand cdna synthesis kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: A comprehensive cancer analysis investigating the oncogenic role of zinc finger protein 36 (ZFP36) in human tumors.
    Article Snippet: .. The cDNA was synthesized from 1 μg total RNA using the SureScript First-Strand cDNA Synthesis Kit (GeneCopoeia, USA) in a 20 μL reaction (Table 1). .. The reaction was incubated at 25°C for 5 min, 42°C for 15 min, and 85°C for 5 min. And qPCR was performed using 2× SYBR Green Master Mix (Servicebio, China) on a SLAN96S system (Hongshi Medical, China).

    Article Title: A comprehensive cancer analysis investigating the oncogenic role of zinc finger protein 36 ( ZFP36 ) in human tumors
    Article Snippet: .. The cDNA was synthesized from 1 μg total RNA using the SureScript First-Strand cDNA Synthesis Kit (GeneCopoeia, USA) in a 20 μL reaction (Table ). .. The reaction was incubated at 25 °C for 5 min, 42 °C for 15 min, and 85 °C for 5 min. And qPCR was performed using 2× SYBR Green Master Mix (Servicebio, China) on a SLAN-96 S system (Hongshi Medical, China).

    Article Title: Transcriptome-based identification and experimental validation of a key gene in Alzheimer's disease using dermal fibroblasts.
    Article Snippet: Background: Alzheimer’s disease (AD) is a neurodegenerative disorder primarily characterized by progressive cognitive impairment and neuronal damage.. The pathogenesis of AD is complex and involves multiple pathological processes.. Currently, effective methods for early diagnosis and treatment are lacking.

    cDNA Synthesis:

    Article Title: A comprehensive cancer analysis investigating the oncogenic role of zinc finger protein 36 (ZFP36) in human tumors.
    Article Snippet: .. The cDNA was synthesized from 1 μg total RNA using the SureScript First-Strand cDNA Synthesis Kit (GeneCopoeia, USA) in a 20 μL reaction (Table 1). .. The reaction was incubated at 25°C for 5 min, 42°C for 15 min, and 85°C for 5 min. And qPCR was performed using 2× SYBR Green Master Mix (Servicebio, China) on a SLAN96S system (Hongshi Medical, China).

    Article Title: ZC3H13-mediated the m6A modification of PAX9 suppresses the progression of reflux esophagitis via improving esophageal epithelial barrier dysfunction.
    Article Snippet: The role of N6-methyladenosine (m6A) in reflux esophagitis (RE) has not been fully elucidated.. We performed MeRIP-seq to profile m6A-modified genes in RE.. The expression of ZC3H13 and PAX9 was analyzed in non-RE and RE tissues using RT-qPCR and western blot, followed by functional assays in human esophageal epithelial cells (HEEC).

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Article Title: Formononetin attenuates cerebral ischemia-reperfusion injury by regulating microglial glycolysis through AMPK.
    Article Snippet: Ethnopharmacological relevance: Astragalus membranaceus (Fisch.). Bunge is a fundamental herb in traditional Chinese medicine.. It has a long history of use in clinical practice for treating stroke and its associated sequelae.

    Article Title: A comprehensive cancer analysis investigating the oncogenic role of zinc finger protein 36 ( ZFP36 ) in human tumors
    Article Snippet: .. The cDNA was synthesized from 1 μg total RNA using the SureScript First-Strand cDNA Synthesis Kit (GeneCopoeia, USA) in a 20 μL reaction (Table ). .. The reaction was incubated at 25 °C for 5 min, 42 °C for 15 min, and 85 °C for 5 min. And qPCR was performed using 2× SYBR Green Master Mix (Servicebio, China) on a SLAN-96 S system (Hongshi Medical, China).

    Article Title: Transcriptome-based identification and experimental validation of a key gene in Alzheimer's disease using dermal fibroblasts.
    Article Snippet: Background: Alzheimer’s disease (AD) is a neurodegenerative disorder primarily characterized by progressive cognitive impairment and neuronal damage.. The pathogenesis of AD is complex and involves multiple pathological processes.. Currently, effective methods for early diagnosis and treatment are lacking.

    Article Title: DYRK2 drives renal fibrosis through CDK1-dependent G2/M phase dysregulation in tubular epithelial cells.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS SureScriptTM First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056T) using 1 μg of total RNA according to the manufacturer's protocol (incubation at 42°C for 15 min, followed by enzyme inactivation at 85°C for 5 min). ..

    Reverse Transcription:

    Article Title: Estrogen upregulates NR4A1 to counter TGF beta induced pulmonary fibrosis therapeutic insights for IPF
    Article Snippet: Total RNA was extracted using TRIzol reagent (GeneCopoeia, QP020, USA), and RNA concentration and purity were assessed with using a NanoDrop spectrophotometer. .. RNA was then reverse transcribed into complementary DNA (cDNA) using the GeneCopoeia reverse transcription kit (QP056, USA). .. Quantitative real-time PCR (qRT-PCR) was performed on a GeneCopoeia real-time PCR system (QP031, China), with each reaction containing 10 μL of 2× SYBR Green PCR Master Mix, 0.4 μL of each specific primer (10 μM), and 1 μL of cDNA template, in a total volume of 20 μL.

    Article Title: ZC3H13-mediated the m6A modification of PAX9 suppresses the progression of reflux esophagitis via improving esophageal epithelial barrier dysfunction.
    Article Snippet: The role of N6-methyladenosine (m6A) in reflux esophagitis (RE) has not been fully elucidated.. We performed MeRIP-seq to profile m6A-modified genes in RE.. The expression of ZC3H13 and PAX9 was analyzed in non-RE and RE tissues using RT-qPCR and western blot, followed by functional assays in human esophageal epithelial cells (HEEC).

    Isolation:

    Article Title: ZC3H13-mediated the m6A modification of PAX9 suppresses the progression of reflux esophagitis via improving esophageal epithelial barrier dysfunction.
    Article Snippet: The role of N6-methyladenosine (m6A) in reflux esophagitis (RE) has not been fully elucidated.. We performed MeRIP-seq to profile m6A-modified genes in RE.. The expression of ZC3H13 and PAX9 was analyzed in non-RE and RE tissues using RT-qPCR and western blot, followed by functional assays in human esophageal epithelial cells (HEEC).

    Real-time Polymerase Chain Reaction:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Article Title: Transcriptome-based identification and experimental validation of a key gene in Alzheimer's disease using dermal fibroblasts.
    Article Snippet: Background: Alzheimer’s disease (AD) is a neurodegenerative disorder primarily characterized by progressive cognitive impairment and neuronal damage.. The pathogenesis of AD is complex and involves multiple pathological processes.. Currently, effective methods for early diagnosis and treatment are lacking.

    CCK-8 Assay:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Inverted Microscopy:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Imaging:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Recombinant:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Immunostaining:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Fluorescence:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Microscopy:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Flow Cytometry:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    TUNEL Assay:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Luciferase:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Reporter Gene Assay:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Plasmid Preparation:

    Article Title: Patchouli alcohol suppresses gastric cancer growth and immune evasion via inhibition of the NF-κB/PD-L1 axis
    Article Snippet: .. SureScript TM First-Strand cDNA Synthesis Kit (Cat: QP056T; GeneCopeia, USA), 2×SYBR Green qPCR Master Mix (None ROX) (Cat: G3320-05; Servicebio, China), TriQuick Reagent (Cat: R1100, Solarbio, China), MKN-45 (CL-0292, Procell, China), HGC-27 (CL-0107, Procell, China), GES-1 (CL-0563, Procell, China), Cell Counting Kit-8 (Cat: G4103, Servicebio, China), Patchouli alcohol (Cat: MUST-24053118, Purity: 99.63%, Must Bio-Technology, China), Multiskan FC Microplate Photometer (DNM-9602, Perlang, China), inverted microscope (OPTOP OD630K, Sunny Hengpin, China), chemiluminescence imaging system (ChemiScope6100, Clin, China), microplate reader (RMR-2208LZN; Ranhui, China), Invitrogen Power Blotter (Model PB0010; Thermo Fisher Scientific, China), anti-NF-kB p65 antibody (ab16502; abcam, UK), NF-κB p65 polyclonal antibody (Cat: 10745-1-AP, Proteintech, China), recombinant anti-PD-L1 antibody [EPR19759] (ab213524, Abcam, UK), PD-L1/CD274 (C-terminal) polyclonal antibody (Cat: 28076-1-AP; Proteintech, China), primary and secondary antibody diluent for immunostaining (Cat: 36206ES76; YEASEN, China), goat anti-rabbit IgG H&L (Alexa Fluor ® 488) (ab150077; abcam, UK), fluorescence microscope (BH2-RFCA; OLYMPUS, Japan), Annexin V-FITC/PI apoptosis kit (Cat: AP101-100-kit, MultiSciences, China), BD FACSCalibur TM Flow Cytometer (E97501093, BD Biosciences, USA), Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (JL10208, Jianglai Biological, China), Human Interferon (IFN-γ) detection kit (JL12152, Jianglai Biological, China), TUNEL Apoptosis Detection Kit-FITC (50T) (MK1027, BOSTER, China), Dual Luciferase Reporter Gene Assay Kit (Cat: RG009, Beyotime, China), Plasmid Mini Preparation Kit (BioDev-Tech, China), pmirGLO (Honeke, China), restriction endonuclease (Thermo, USA), and GloMax ® 96 Microplate Luminometer (Promega, USA). .. Human gastric mucosal epithelial cells (GES-1), gastric cancer cell lines (AGS, BGC823, NUGC-4, SGC7901, MKN-45, HGC-27), and MFC cells were purchased from Procell Systems.

    Generated:

    Article Title: Formononetin attenuates cerebral ischemia-reperfusion injury by regulating microglial glycolysis through AMPK.
    Article Snippet: Ethnopharmacological relevance: Astragalus membranaceus (Fisch.). Bunge is a fundamental herb in traditional Chinese medicine.. It has a long history of use in clinical practice for treating stroke and its associated sequelae.

    Quantitative RT-PCR:

    Article Title: Transcriptome-based identification and experimental validation of a key gene in Alzheimer's disease using dermal fibroblasts.
    Article Snippet: Background: Alzheimer’s disease (AD) is a neurodegenerative disorder primarily characterized by progressive cognitive impairment and neuronal damage.. The pathogenesis of AD is complex and involves multiple pathological processes.. Currently, effective methods for early diagnosis and treatment are lacking.

    Incubation:

    Article Title: DYRK2 drives renal fibrosis through CDK1-dependent G2/M phase dysregulation in tubular epithelial cells.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS SureScriptTM First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056T) using 1 μg of total RNA according to the manufacturer's protocol (incubation at 42°C for 15 min, followed by enzyme inactivation at 85°C for 5 min). ..



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