Journal: Molecular Therapy Oncology

Article Title: A monoclonal antibody W1 blocks mesothelin-mediated tumor progression

doi: 10.1016/j.omton.2025.201094

Figure Lengend Snippet: MSLN and D1 domain promote ovarian cancer cell migration and invasion through the AKT-MMP7 signaling pathway Wound-healing assays showing migration of OVCAR3 MSLN KO (A) and SKOV3 (B) cells after 48 h treatment with MSLN-Fc, D1-Fc, D2-Fc, D3-Fc, or Fc. Data were normalized to the PBS group. Invasion assays of OVCAR3 MSLN KO (C) and SKOV3 (D) cells under the same treatment conditions for 48 h. RT-qPCR analysis of Mmp7 and Mmp9 mRNA expression in response to MSLN-Fc stimulation over time (E) and at increasing concentrations (F). Western blot showing MMP7 protein expression induced by escalating doses of MSLN-Fc, with Fc as control (G). Western blot analysis of MMP7, E-cadherin, and AKT phosphorylation in OVCAR3 MSLN KO (H) and SKOV3 (I) cells treated for 24 h with 100 nM MSLN-Fc, D1-Fc, D2-Fc, D3-Fc, or Fc. All quantitative data are presented as mean ± SD from three biologically independent experiments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; data were analyzed by ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: RNA was extracted from different groups of cells using a total RNA kit I (Omega, cat#R6834-01), and cDNA was obtained through reverse transcription using HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme, cat#R333-01).

Techniques: Migration, Quantitative RT-PCR, Expressing, Western Blot, Control, Phospho-proteomics, Comparison

Journal: Molecular Therapy Oncology

Article Title: A monoclonal antibody W1 blocks mesothelin-mediated tumor progression

doi: 10.1016/j.omton.2025.201094

Figure Lengend Snippet: W1 mIgG1 inhibits ovarian cancer cell migration and invasion in a dose-dependent manner by downregulating MMP7 (A) Wound-healing assay showing the migration of OVCAR3 cells treated with increasing concentrations of W1 mIgG1 (100, 300, 600 nM). Data were normalized to the PBS group. (B) Transwell invasion assay demonstrating the dose-dependent suppression of OVCAR3 cell invasion by W1 mIgG1. RT-qPCR (C) and Western blot (D) analyses revealed that W1 mIgG1 treatment progressively reduced Mmp7 expression at both mRNA and protein levels in OVCAR3 cells, whereas A12H cIgG1 (300 nM) showed no effect. Comparative effects of W1 mIgG1 (300 nM) and the MMPi (1,000 nM) on MSLN-induced cell migration (E) and invasion (F). Both treatments showed similar inhibitory efficacy. All quantitative data are presented as mean ± SD from three biologically independent experiments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; data were analyzed by ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: RNA was extracted from different groups of cells using a total RNA kit I (Omega, cat#R6834-01), and cDNA was obtained through reverse transcription using HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme, cat#R333-01).

Techniques: Migration, Wound Healing Assay, Transwell Invasion Assay, Quantitative RT-PCR, Western Blot, Expressing, Comparison