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Proteintech stx6

<t>STX6</t> is a target of miR‐375‐3p in endothelial cell senescence. (a) Predicted and mutated miR‐375‐3p binding sites within the 3′UTR of STX6. (b) Luciferase assay of WT and mutant STX6 3′UTR reporters in HEK293A cells transfected with miR‐375‐3p mimic or scramble. (c) qRT‐PCR analysis of STX6 mRNA in HUVECs transfected with miR‐375‐3p mimic or scramble, normalized to GAPDH. (d, e) Western blot analysis of STX6 protein expression in HUVECs transfected with miR‐375‐3p mimic (d) or STX6 siRNAs (e), normalized to GAPDH. (f) Representative SA‐β‐gal staining and quantification in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 200 μm. (g) Representative colony formation images showing cell proliferation in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 5 mm. (h) qRT‐PCR analysis of p15, IL6, and IL8 mRNA in HUVECs transfected with STX6 siRNAs or scramble, normalized to GAPDH. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test. ** p < 0.01, *** p < 0.001.

Stx6, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stx6/pmc12741199-163-4-6?v=Proteintech
Average 93 stars, based on 22 article reviews

stx6 - by Bioz Stars, 2026-07

93/100 stars

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1) Product Images from "miR ‐375‐3p/ STX6 Exacerbates Atherosclerosis by Promoting Endothelial Cell Senescence via Activation of TGF ‐Beta Signals"

Article Title: miR ‐375‐3p/ STX6 Exacerbates Atherosclerosis by Promoting Endothelial Cell Senescence via Activation of TGF ‐Beta Signals

Journal: Aging Cell

doi: 10.1111/acel.70326

STX6 is a target of miR‐375‐3p in endothelial cell senescence. (a) Predicted and mutated miR‐375‐3p binding sites within the 3′UTR of STX6. (b) Luciferase assay of WT and mutant STX6 3′UTR reporters in HEK293A cells transfected with miR‐375‐3p mimic or scramble. (c) qRT‐PCR analysis of STX6 mRNA in HUVECs transfected with miR‐375‐3p mimic or scramble, normalized to GAPDH. (d, e) Western blot analysis of STX6 protein expression in HUVECs transfected with miR‐375‐3p mimic (d) or STX6 siRNAs (e), normalized to GAPDH. (f) Representative SA‐β‐gal staining and quantification in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 200 μm. (g) Representative colony formation images showing cell proliferation in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 5 mm. (h) qRT‐PCR analysis of p15, IL6, and IL8 mRNA in HUVECs transfected with STX6 siRNAs or scramble, normalized to GAPDH. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test. ** p < 0.01, *** p < 0.001.

Figure Legend Snippet: STX6 is a target of miR‐375‐3p in endothelial cell senescence. (a) Predicted and mutated miR‐375‐3p binding sites within the 3′UTR of STX6. (b) Luciferase assay of WT and mutant STX6 3′UTR reporters in HEK293A cells transfected with miR‐375‐3p mimic or scramble. (c) qRT‐PCR analysis of STX6 mRNA in HUVECs transfected with miR‐375‐3p mimic or scramble, normalized to GAPDH. (d, e) Western blot analysis of STX6 protein expression in HUVECs transfected with miR‐375‐3p mimic (d) or STX6 siRNAs (e), normalized to GAPDH. (f) Representative SA‐β‐gal staining and quantification in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 200 μm. (g) Representative colony formation images showing cell proliferation in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 5 mm. (h) qRT‐PCR analysis of p15, IL6, and IL8 mRNA in HUVECs transfected with STX6 siRNAs or scramble, normalized to GAPDH. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test. ** p < 0.01, *** p < 0.001.

Techniques Used: Binding Assay, Luciferase, Mutagenesis, Transfection, Quantitative RT-PCR, Western Blot, Expressing, Staining, Two Tailed Test

STX6 overexpression rescues the pro‐senescent effect of miR‐375‐3p. (a) Representative SA‐β‐gal staining and quantification in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic. Scale bar = 200 μm. (b) qRT‐PCR analysis of p15, IL6, and IL8 mRNA expression in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic, normalized to GAPDH. (c) Colony formation assay showing cell proliferation in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic. Scale bar = 5 mm. Data are presented as mean ± SD; statistical significance determined by two‐way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001.

Figure Legend Snippet: STX6 overexpression rescues the pro‐senescent effect of miR‐375‐3p. (a) Representative SA‐β‐gal staining and quantification in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic. Scale bar = 200 μm. (b) qRT‐PCR analysis of p15, IL6, and IL8 mRNA expression in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic, normalized to GAPDH. (c) Colony formation assay showing cell proliferation in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic. Scale bar = 5 mm. Data are presented as mean ± SD; statistical significance determined by two‐way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques Used: Over Expression, Staining, Infection, Transfection, Quantitative RT-PCR, Expressing, Colony Assay

miR‐375‐3p/STX6 signaling promotes endothelial cell senescence through the increased internalization of TGFBR1. (a) Immunofluorescence images showing colocalization (yellow) of TGFBR1 (green) and EEA1 (red) in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p. Nuclei stained with Hoechst (blue). Scale bar = 5 μm. (b–d) Western blot analysis of SMAD2 phosphorylation and p15 expression in HUVECs transfected with miR‐375‐3p mimic or STX6 siRNAs (b), infected with Ad‐STX6 and transfected with miR‐375‐3p mimic (c), or co‐transfected with SMAD2 siRNA and miR‐375‐3p mimic or STX6 siRNAs (d). (e) Representative SA‐β‐gal staining and quantification in HUVECs co‐transfected with SMAD2 siRNA and miR‐375‐3p mimic or STX6 siRNAs. Scale bar = 200 μm. (f, g) Western blot (f) and SA‐β‐gal (g) staining in HUVECs infected with Ad‐STX6 and stimulated with TGF‐β1. Scale bar = 200 μm. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test or two‐way ANOVA. ** p < 0.01, *** p < 0.001.

Figure Legend Snippet: miR‐375‐3p/STX6 signaling promotes endothelial cell senescence through the increased internalization of TGFBR1. (a) Immunofluorescence images showing colocalization (yellow) of TGFBR1 (green) and EEA1 (red) in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p. Nuclei stained with Hoechst (blue). Scale bar = 5 μm. (b–d) Western blot analysis of SMAD2 phosphorylation and p15 expression in HUVECs transfected with miR‐375‐3p mimic or STX6 siRNAs (b), infected with Ad‐STX6 and transfected with miR‐375‐3p mimic (c), or co‐transfected with SMAD2 siRNA and miR‐375‐3p mimic or STX6 siRNAs (d). (e) Representative SA‐β‐gal staining and quantification in HUVECs co‐transfected with SMAD2 siRNA and miR‐375‐3p mimic or STX6 siRNAs. Scale bar = 200 μm. (f, g) Western blot (f) and SA‐β‐gal (g) staining in HUVECs infected with Ad‐STX6 and stimulated with TGF‐β1. Scale bar = 200 μm. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test or two‐way ANOVA. ** p < 0.01, *** p < 0.001.

Techniques Used: Immunofluorescence, Infection, Transfection, Staining, Western Blot, Phospho-proteomics, Expressing, Two Tailed Test

Overexpression of STX6 reduce atherosclerotic plaque in mice. (a) qRT‐PCR analysis of Stx6 expression in aortas of WT ( n = 21) and ApoE −/− ( n = 23) mice fed HFD for 8 weeks, normalized to Gapdh. (b) Schematic of experimental design showing Ad‐Ctrl or Ad‐STX6 injection (1 × 10 9 PFU per mouse, twice weekly for 8 weeks) in ApoE −/− mice on HFD. (c) Representative Oil Red O staining images and quantification of lesion size and positive area in the aortic sinus. Scale bar = 200 μm. (d) Representative Oil Red O staining images and quantification of plaque area in the entire aortic tree. (e) Immunofluorescence staining of macrophage marker MOMA2 (green) in aortic sinus. Scale bar = 200 μm. (f) Representative SA‐β‐gal and Cd31 co‐staining in aortic sinus lesions. Scale bar = 5 μm. (g) Immunofluorescence of phosphorylated Smad2 (green) and Cd31 (red) in the aortic intima (L). Nuclei stained with Hoechst (blue). Scale bar = 5 μm. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test. * p < 0.05, ** p < 0.01.

Figure Legend Snippet: Overexpression of STX6 reduce atherosclerotic plaque in mice. (a) qRT‐PCR analysis of Stx6 expression in aortas of WT ( n = 21) and ApoE −/− ( n = 23) mice fed HFD for 8 weeks, normalized to Gapdh. (b) Schematic of experimental design showing Ad‐Ctrl or Ad‐STX6 injection (1 × 10 9 PFU per mouse, twice weekly for 8 weeks) in ApoE −/− mice on HFD. (c) Representative Oil Red O staining images and quantification of lesion size and positive area in the aortic sinus. Scale bar = 200 μm. (d) Representative Oil Red O staining images and quantification of plaque area in the entire aortic tree. (e) Immunofluorescence staining of macrophage marker MOMA2 (green) in aortic sinus. Scale bar = 200 μm. (f) Representative SA‐β‐gal and Cd31 co‐staining in aortic sinus lesions. Scale bar = 5 μm. (g) Immunofluorescence of phosphorylated Smad2 (green) and Cd31 (red) in the aortic intima (L). Nuclei stained with Hoechst (blue). Scale bar = 5 μm. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test. * p < 0.05, ** p < 0.01.

Techniques Used: Over Expression, Quantitative RT-PCR, Expressing, Injection, Staining, Immunofluorescence, Marker, Two Tailed Test

Image Search Results

A) RT-qPCR of RNA isolated from Missense control and Stx6 knockdown (KD) BEAS-2Bs. Stx6 expression was calculated relative to Gapdh. B) IFN-γ ELISpot assay with Mtb infected Missense control and Stx6 knockdown BEAS-2Bs using MR1-restricted MAIT cell clone, p=0.7413. MAIT cell responses measured by mean IFN-γ spot forming units (SFU). C) RT-qPCR of RNA isolated from Missense control and Stx12 knockdown BEAS-2Bs. Stx12 expression was calculated relative to Gapdh . D) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx12 knockdown BEAS-2Bs using MR1-restricted MAIT cell clone, p=0.0333. E) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx12 knockdown BEAS-2Bs using HLA-B45-restricted T cell clone D466-D6, p=0.3375. F) RT-qPCR of RNA isolated from Missense control and Stx16 knockdown BEAS-2Bs. Stx16 expression was calculated relative to Gapdh . G) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx16 knockdown BEAS-2Bs using MR1-restricted MAIT cell clone, p<0.0001. H) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx16 knockdown BEAS-2Bs using HLA-B45-restricted T cell clone D466-A10, p=0.2345. ELISpot assay data are pooled from n=3 independent experiments. Error bars represent standard error of the mean (SEM). Top and EC 50 SFU values were determined by least-squares regression and used to calculate p-values by extra sum-of-squares F test.

Journal: bioRxiv

Article Title: Sorting endosomes play key roles in presentation of Mycobacterium tuberculosis -derived ligands to MAIT cells

doi: 10.64898/2025.12.03.691670

Figure Lengend Snippet: A) RT-qPCR of RNA isolated from Missense control and Stx6 knockdown (KD) BEAS-2Bs. Stx6 expression was calculated relative to Gapdh. B) IFN-γ ELISpot assay with Mtb infected Missense control and Stx6 knockdown BEAS-2Bs using MR1-restricted MAIT cell clone, p=0.7413. MAIT cell responses measured by mean IFN-γ spot forming units (SFU). C) RT-qPCR of RNA isolated from Missense control and Stx12 knockdown BEAS-2Bs. Stx12 expression was calculated relative to Gapdh . D) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx12 knockdown BEAS-2Bs using MR1-restricted MAIT cell clone, p=0.0333. E) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx12 knockdown BEAS-2Bs using HLA-B45-restricted T cell clone D466-D6, p=0.3375. F) RT-qPCR of RNA isolated from Missense control and Stx16 knockdown BEAS-2Bs. Stx16 expression was calculated relative to Gapdh . G) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx16 knockdown BEAS-2Bs using MR1-restricted MAIT cell clone, p<0.0001. H) IFN-γ ELISpot mean SFU from Mtb infected Missense control and Stx16 knockdown BEAS-2Bs using HLA-B45-restricted T cell clone D466-A10, p=0.2345. ELISpot assay data are pooled from n=3 independent experiments. Error bars represent standard error of the mean (SEM). Top and EC 50 SFU values were determined by least-squares regression and used to calculate p-values by extra sum-of-squares F test.

Article Snippet: RT-qPCR was performed using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific) and Taqman FAM-MGB Gene expression Assay probes (Thermo Fisher Scientific) to detect transcripts of Stx6 (Hs01057343_m1), Stx12 (Hs00295291_m1), Stx16 (Hs01002372_m1), MR1 (Hs00155420_m1), and GAPDH (Hs02758991_g1) as an internal reference.

Techniques: Quantitative RT-PCR, Isolation, Control, Knockdown, Expressing, Enzyme-linked Immunospot, Infection

Journal: Aging Cell

Article Title: miR ‐375‐3p/ STX6 Exacerbates Atherosclerosis by Promoting Endothelial Cell Senescence via Activation of TGF ‐Beta Signals

doi: 10.1111/acel.70326

Figure Lengend Snippet: STX6 is a target of miR‐375‐3p in endothelial cell senescence. (a) Predicted and mutated miR‐375‐3p binding sites within the 3′UTR of STX6. (b) Luciferase assay of WT and mutant STX6 3′UTR reporters in HEK293A cells transfected with miR‐375‐3p mimic or scramble. (c) qRT‐PCR analysis of STX6 mRNA in HUVECs transfected with miR‐375‐3p mimic or scramble, normalized to GAPDH. (d, e) Western blot analysis of STX6 protein expression in HUVECs transfected with miR‐375‐3p mimic (d) or STX6 siRNAs (e), normalized to GAPDH. (f) Representative SA‐β‐gal staining and quantification in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 200 μm. (g) Representative colony formation images showing cell proliferation in HUVECs transfected with STX6 siRNAs or scramble. Scale bar = 5 mm. (h) qRT‐PCR analysis of p15, IL6, and IL8 mRNA in HUVECs transfected with STX6 siRNAs or scramble, normalized to GAPDH. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test. ** p < 0.01, *** p < 0.001.

Article Snippet: The primary antibodies included STX6, GAPDH (Proteintech, Wuhan, Hubei, China), p‐SMAD2, SMAD2 (Cell Signaling Technology, Boston, MA, USA), p15 (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Binding Assay, Luciferase, Mutagenesis, Transfection, Quantitative RT-PCR, Western Blot, Expressing, Staining, Two Tailed Test

Journal: Aging Cell

Article Title: miR ‐375‐3p/ STX6 Exacerbates Atherosclerosis by Promoting Endothelial Cell Senescence via Activation of TGF ‐Beta Signals

doi: 10.1111/acel.70326

Figure Lengend Snippet: STX6 overexpression rescues the pro‐senescent effect of miR‐375‐3p. (a) Representative SA‐β‐gal staining and quantification in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic. Scale bar = 200 μm. (b) qRT‐PCR analysis of p15, IL6, and IL8 mRNA expression in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic, normalized to GAPDH. (c) Colony formation assay showing cell proliferation in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p mimic. Scale bar = 5 mm. Data are presented as mean ± SD; statistical significance determined by two‐way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques: Over Expression, Staining, Infection, Transfection, Quantitative RT-PCR, Expressing, Colony Assay

Journal: Aging Cell

Article Title: miR ‐375‐3p/ STX6 Exacerbates Atherosclerosis by Promoting Endothelial Cell Senescence via Activation of TGF ‐Beta Signals

doi: 10.1111/acel.70326

Figure Lengend Snippet: miR‐375‐3p/STX6 signaling promotes endothelial cell senescence through the increased internalization of TGFBR1. (a) Immunofluorescence images showing colocalization (yellow) of TGFBR1 (green) and EEA1 (red) in HUVECs infected with Ad‐STX6 and transfected with miR‐375‐3p. Nuclei stained with Hoechst (blue). Scale bar = 5 μm. (b–d) Western blot analysis of SMAD2 phosphorylation and p15 expression in HUVECs transfected with miR‐375‐3p mimic or STX6 siRNAs (b), infected with Ad‐STX6 and transfected with miR‐375‐3p mimic (c), or co‐transfected with SMAD2 siRNA and miR‐375‐3p mimic or STX6 siRNAs (d). (e) Representative SA‐β‐gal staining and quantification in HUVECs co‐transfected with SMAD2 siRNA and miR‐375‐3p mimic or STX6 siRNAs. Scale bar = 200 μm. (f, g) Western blot (f) and SA‐β‐gal (g) staining in HUVECs infected with Ad‐STX6 and stimulated with TGF‐β1. Scale bar = 200 μm. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test or two‐way ANOVA. ** p < 0.01, *** p < 0.001.

Techniques: Immunofluorescence, Infection, Transfection, Staining, Western Blot, Phospho-proteomics, Expressing, Two Tailed Test

Journal: Aging Cell

Article Title: miR ‐375‐3p/ STX6 Exacerbates Atherosclerosis by Promoting Endothelial Cell Senescence via Activation of TGF ‐Beta Signals

doi: 10.1111/acel.70326

Figure Lengend Snippet: Overexpression of STX6 reduce atherosclerotic plaque in mice. (a) qRT‐PCR analysis of Stx6 expression in aortas of WT ( n = 21) and ApoE −/− ( n = 23) mice fed HFD for 8 weeks, normalized to Gapdh. (b) Schematic of experimental design showing Ad‐Ctrl or Ad‐STX6 injection (1 × 10 9 PFU per mouse, twice weekly for 8 weeks) in ApoE −/− mice on HFD. (c) Representative Oil Red O staining images and quantification of lesion size and positive area in the aortic sinus. Scale bar = 200 μm. (d) Representative Oil Red O staining images and quantification of plaque area in the entire aortic tree. (e) Immunofluorescence staining of macrophage marker MOMA2 (green) in aortic sinus. Scale bar = 200 μm. (f) Representative SA‐β‐gal and Cd31 co‐staining in aortic sinus lesions. Scale bar = 5 μm. (g) Immunofluorescence of phosphorylated Smad2 (green) and Cd31 (red) in the aortic intima (L). Nuclei stained with Hoechst (blue). Scale bar = 5 μm. Data are presented as mean ± SD; statistical significance determined by unpaired two‐tailed Student's t ‐test. * p < 0.05, ** p < 0.01.

Techniques: Over Expression, Quantitative RT-PCR, Expressing, Injection, Staining, Immunofluorescence, Marker, Two Tailed Test

STX6 is upregulated in HCC patients and correlates to HCC progression. ( A ) Results of STX6 expression level analysis in human HCC tissues ( n = 347) and normal tissues ( n = 50) in TCGA databases. ( B ) STX6 expression levels in HCC samples of different histologic grades in TCGA databases. Histologic grading grade I, n = 60; grade II, n = 205; grade III, n = 139; grade IV, n = 12. ( C ) STX6 mRNA levels in HCC samples with different TNM stages in TCGA databases. Stage I, n = 191; Stage II, n = 98; Stage III, n = 97; Stage IV, n = 6. ( D ) Kaplan-Meier overall survival curves of HCC patients with high and low STX6 expression in the TCGA database. ( E ) STX6 mRNA levels in paired HCC and paracancerous tissues. β-actin was used to normalize. Data was given as mean ± SD. ( F ) The WB and quantification results of STX6 in HCC tissues and paracancerous tissues. GAPDH served as the loading control. Data was given as mean ± SD. ( G ) Results of IHC analysis of STX6 in HCC tissues and paracancerous tissues. Data was given as mean ± SD. For statistical analysis, two-tailed Student’s t -test was used in E - G . * P < 0.05; ** P < 0.01

Journal: Cancer Cell International

Article Title: Syntaxin6 contributes to hepatocellular carcinoma tumorigenesis via enhancing STAT3 phosphorylation

doi: 10.1186/s12935-024-03377-3

Figure Lengend Snippet: STX6 is upregulated in HCC patients and correlates to HCC progression. ( A ) Results of STX6 expression level analysis in human HCC tissues ( n = 347) and normal tissues ( n = 50) in TCGA databases. ( B ) STX6 expression levels in HCC samples of different histologic grades in TCGA databases. Histologic grading grade I, n = 60; grade II, n = 205; grade III, n = 139; grade IV, n = 12. ( C ) STX6 mRNA levels in HCC samples with different TNM stages in TCGA databases. Stage I, n = 191; Stage II, n = 98; Stage III, n = 97; Stage IV, n = 6. ( D ) Kaplan-Meier overall survival curves of HCC patients with high and low STX6 expression in the TCGA database. ( E ) STX6 mRNA levels in paired HCC and paracancerous tissues. β-actin was used to normalize. Data was given as mean ± SD. ( F ) The WB and quantification results of STX6 in HCC tissues and paracancerous tissues. GAPDH served as the loading control. Data was given as mean ± SD. ( G ) Results of IHC analysis of STX6 in HCC tissues and paracancerous tissues. Data was given as mean ± SD. For statistical analysis, two-tailed Student’s t -test was used in E - G . * P < 0.05; ** P < 0.01

Article Snippet: The used primary antibodies were as follows: GAPDH (Proteintech, 60004-1), STX6 (Abclonal, A19813), ACTIN (Abclonal, AC026), PCNA (Biolight, CP00140HuA10), CYCLIND1 (Abclonal, A22104), E-CAD (Biolight, RMAP0043M1), N-CAD (Abclonal, A3045), p-STAT3 (CST, 9145 S), STAT3(CST, 12,640 S), RACK1 (CST, 5432 S).

Techniques: Expressing, Two Tailed Test

STX6 increases the proliferative capacity of the HCC cells. ( A ) STX6 protein expression level in Huh7/HepG2 STX6-OE and corresponding control cells. ( B ) Results of CCK8 experiments in Huh7/HepG2 STX6-OE and corresponding control cell groups. n = 5 per group. ( C ) The representative images and quantification results of Huh7/HepG2 STX6-OE and corresponding control cells in cell colony formation assay. n = 3 per group. ( D ) Protein levels and quantification results of proliferation-related molecules PCNA and cyclin D1 in Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. β-actin served as the loading control. ( E ) STX6 protein expression level in Huh7/HepG2 sh STX6 and corresponding control cells. ( F ) Results of CCK8 experiments in Huh7/HepG2 sh STX6 and corresponding control cell groups. n = 5 per group. ( G ) The representative images and quantification results of Huh7/HepG2 sh STX6 and corresponding control cells in cell colony formation assay. n = 3 per group. ( H ) Protein levels and quantification results of proliferation-related molecules PCNA and cyclin D1 in Huh7/HepG2 sh STX6 and corresponding control cells. n = 3 per group. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in B - D and F - H . n.s. indicates no significant difference; * P < 0.05; ** P < 0.01

Journal: Cancer Cell International

Article Title: Syntaxin6 contributes to hepatocellular carcinoma tumorigenesis via enhancing STAT3 phosphorylation

doi: 10.1186/s12935-024-03377-3

Figure Lengend Snippet: STX6 increases the proliferative capacity of the HCC cells. ( A ) STX6 protein expression level in Huh7/HepG2 STX6-OE and corresponding control cells. ( B ) Results of CCK8 experiments in Huh7/HepG2 STX6-OE and corresponding control cell groups. n = 5 per group. ( C ) The representative images and quantification results of Huh7/HepG2 STX6-OE and corresponding control cells in cell colony formation assay. n = 3 per group. ( D ) Protein levels and quantification results of proliferation-related molecules PCNA and cyclin D1 in Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. β-actin served as the loading control. ( E ) STX6 protein expression level in Huh7/HepG2 sh STX6 and corresponding control cells. ( F ) Results of CCK8 experiments in Huh7/HepG2 sh STX6 and corresponding control cell groups. n = 5 per group. ( G ) The representative images and quantification results of Huh7/HepG2 sh STX6 and corresponding control cells in cell colony formation assay. n = 3 per group. ( H ) Protein levels and quantification results of proliferation-related molecules PCNA and cyclin D1 in Huh7/HepG2 sh STX6 and corresponding control cells. n = 3 per group. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in B - D and F - H . n.s. indicates no significant difference; * P < 0.05; ** P < 0.01

Techniques: Expressing, Colony Assay, Two Tailed Test

STX6 promotes migration and invasion of HCC cells. ( A ) The representative images and quantification results of Transwell migration assay of Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. ( B ) The representative images and quantification results of Huh7 STX6-OE and corresponding control cells. n = 3 per group in wound healing assay. ( C ) The representative images and quantification results of Huh7/HepG2-STX6-OE and corresponding control cells in Transwell invasion assay. n = 3 per group. ( D - E ) Protein levels and quantification results of metastasis-related E-cadherin and N-cadherin in Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. ( F ) The representative images and quantification results of Huh7/HepG2 sh STX6 and corresponding control cells in Transwell migration assay. n = 3 per group. ( G ) The representative images and quantification results of wound healing assay of Huh7 sh STX6 and corresponding control cells. n = 3 per group. ( H ) The representative images and quantification results of Transwell invasion assay of Huh7/HepG2 sh STX6 and corresponding control cells. n = 3 per group. ( I - J ) Protein levels and quantification results of metastasis-related E-cadherin and N-cadherin in Huh7/HepG2 sh STX6 and corresponding control cells. n = 3 per group. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in A - C , E - H , and J . * P < 0.05; ** P < 0.01

Journal: Cancer Cell International

Article Title: Syntaxin6 contributes to hepatocellular carcinoma tumorigenesis via enhancing STAT3 phosphorylation

doi: 10.1186/s12935-024-03377-3

Figure Lengend Snippet: STX6 promotes migration and invasion of HCC cells. ( A ) The representative images and quantification results of Transwell migration assay of Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. ( B ) The representative images and quantification results of Huh7 STX6-OE and corresponding control cells. n = 3 per group in wound healing assay. ( C ) The representative images and quantification results of Huh7/HepG2-STX6-OE and corresponding control cells in Transwell invasion assay. n = 3 per group. ( D - E ) Protein levels and quantification results of metastasis-related E-cadherin and N-cadherin in Huh7/HepG2 STX6-OE and corresponding control cells. n = 3 per group. ( F ) The representative images and quantification results of Huh7/HepG2 sh STX6 and corresponding control cells in Transwell migration assay. n = 3 per group. ( G ) The representative images and quantification results of wound healing assay of Huh7 sh STX6 and corresponding control cells. n = 3 per group. ( H ) The representative images and quantification results of Transwell invasion assay of Huh7/HepG2 sh STX6 and corresponding control cells. n = 3 per group. ( I - J ) Protein levels and quantification results of metastasis-related E-cadherin and N-cadherin in Huh7/HepG2 sh STX6 and corresponding control cells. n = 3 per group. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in A - C , E - H , and J . * P < 0.05; ** P < 0.01

Techniques: Migration, Transwell Migration Assay, Wound Healing Assay, Transwell Invasion Assay, Two Tailed Test

STX6 promotes HCC tumorigenic behavior in vivo. ( A ) Representative images of HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 6 per group. ( B ) STX6 expression in HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. ( C ) Results of tumor volume analysis at different days after HepG2 cells’ injection. n = 7 per group. ( D ) Tumor weight of HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 7 per group. ( E - F ) The representative images ( E ) and quantification results ( F ) of immunohistochemical staining of PCNA and Ki-67 in the HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 4 per group. ( G - H ) Protein levels ( G ) and quantification results ( H ) of PCNA, cyclin D1, E-cadherin and N-cadherin in HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 3 per group. β-actin served as the loading control. Data was given as mean ± SD. ( I ) Representative images of Huh7 shRNA- and Huh7 sh STX6 -derived xenograft tumors. n = 6 per group. ( J ) Results of tumor volume analysis at different days after Huh7 cells’ injection. n = 7 per group. ( K ) Tumor weight of Huh7 shRNA- and Huh7 sh STX6 -derived xenograft tumors. n = 7 per group. ( L ) The representative images and quantification results of immunohistochemical staining of PCNA and Ki-67 in the Huh7 shRNA- and Huh7 sh STX6 -derived xenograft tumors. n = 4 per group. For statistical analysis, the two-tailed Student’s t -test was used in C , D , F , H and J - L . n.s. indicates no significant difference; * P < 0.05; ** P < 0.01

Journal: Cancer Cell International

Article Title: Syntaxin6 contributes to hepatocellular carcinoma tumorigenesis via enhancing STAT3 phosphorylation

doi: 10.1186/s12935-024-03377-3

Figure Lengend Snippet: STX6 promotes HCC tumorigenic behavior in vivo. ( A ) Representative images of HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 6 per group. ( B ) STX6 expression in HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. ( C ) Results of tumor volume analysis at different days after HepG2 cells’ injection. n = 7 per group. ( D ) Tumor weight of HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 7 per group. ( E - F ) The representative images ( E ) and quantification results ( F ) of immunohistochemical staining of PCNA and Ki-67 in the HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 4 per group. ( G - H ) Protein levels ( G ) and quantification results ( H ) of PCNA, cyclin D1, E-cadherin and N-cadherin in HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. n = 3 per group. β-actin served as the loading control. Data was given as mean ± SD. ( I ) Representative images of Huh7 shRNA- and Huh7 sh STX6 -derived xenograft tumors. n = 6 per group. ( J ) Results of tumor volume analysis at different days after Huh7 cells’ injection. n = 7 per group. ( K ) Tumor weight of Huh7 shRNA- and Huh7 sh STX6 -derived xenograft tumors. n = 7 per group. ( L ) The representative images and quantification results of immunohistochemical staining of PCNA and Ki-67 in the Huh7 shRNA- and Huh7 sh STX6 -derived xenograft tumors. n = 4 per group. For statistical analysis, the two-tailed Student’s t -test was used in C , D , F , H and J - L . n.s. indicates no significant difference; * P < 0.05; ** P < 0.01

Techniques: In Vivo, shRNA, Derivative Assay, Expressing, Injection, Immunohistochemical staining, Staining, Two Tailed Test

STX6 regulates the STAT3 signaling pathway. ( A ) Cluster analysis of RNA-Seq results from HepG2 STX6 knockdown and control cell lines. ( B ) Gene set enrichment analysis of RNA-Seq results from HepG2 STX6 knockdown and control cell lines. ( C - D ) Gene expression levels associated with MYC, reactive oxygen species pathway, KRAS signaling pathway, oxidative phosphorylation, EMT, and DNA repair in HepG2 STX6 knockdown and control cell lines. ( E ) KEGG pathway enrichment analysis in HepG2-sh STX6 cells. ( F ) IP-MS experiment was used to identify the potential targets of STX6 in Falg-STX6-overexpressed HepG2 cells. ( G ) Co-IP analysis of the interaction between STX6 and RACK1 from IP-MS in 293T cells. ( H ) Co-IP analysis of the interaction between STX6 and STAT3 in 293T cells. ( I - J ) Total and phosphorylated STAT3 protein levels in Huh7/HepG2 STX6-OE and its control cells. β-actin served as the loading control. ( K - L ) Total and phosphorylated STAT3 protein levels in Huh7/HepG2 sh STX6 and its control cells. β-actin served as the loading control. ( M ) Total and phosphorylated STAT3 protein levels in HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in I - M . * P < 0.05; ** P < 0.01

Journal: Cancer Cell International

Article Title: Syntaxin6 contributes to hepatocellular carcinoma tumorigenesis via enhancing STAT3 phosphorylation

doi: 10.1186/s12935-024-03377-3

Figure Lengend Snippet: STX6 regulates the STAT3 signaling pathway. ( A ) Cluster analysis of RNA-Seq results from HepG2 STX6 knockdown and control cell lines. ( B ) Gene set enrichment analysis of RNA-Seq results from HepG2 STX6 knockdown and control cell lines. ( C - D ) Gene expression levels associated with MYC, reactive oxygen species pathway, KRAS signaling pathway, oxidative phosphorylation, EMT, and DNA repair in HepG2 STX6 knockdown and control cell lines. ( E ) KEGG pathway enrichment analysis in HepG2-sh STX6 cells. ( F ) IP-MS experiment was used to identify the potential targets of STX6 in Falg-STX6-overexpressed HepG2 cells. ( G ) Co-IP analysis of the interaction between STX6 and RACK1 from IP-MS in 293T cells. ( H ) Co-IP analysis of the interaction between STX6 and STAT3 in 293T cells. ( I - J ) Total and phosphorylated STAT3 protein levels in Huh7/HepG2 STX6-OE and its control cells. β-actin served as the loading control. ( K - L ) Total and phosphorylated STAT3 protein levels in Huh7/HepG2 sh STX6 and its control cells. β-actin served as the loading control. ( M ) Total and phosphorylated STAT3 protein levels in HepG2 shRNA- and HepG2 sh STX6 -derived xenograft tumors. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in I - M . * P < 0.05; ** P < 0.01

Techniques: RNA Sequencing Assay, Expressing, Co-Immunoprecipitation Assay, shRNA, Derivative Assay, Two Tailed Test

STAT3 phosphorylation mediated the promotive role of STX6 in HCC progression. ( A ) Protein levels and quantification results of total and phosphorylated STAT3 in control and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO. β-actin served as the loading control. ( B ) The representative images and quantification results of control and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO in colony formation assays. n = 3 per group. ( C ) The representative images and quantification results of control cells and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO in Transwell migration assays. n = 6 per group. ( D ) Protein levels and quantification results of PCNA, cyclin D1, E-cadherin and N-cadherin in control and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO. n = 6 per group. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in A - D . n.s. indicates no significant difference; * P < 0.05; ** P < 0.01

Journal: Cancer Cell International

Article Title: Syntaxin6 contributes to hepatocellular carcinoma tumorigenesis via enhancing STAT3 phosphorylation

doi: 10.1186/s12935-024-03377-3

Figure Lengend Snippet: STAT3 phosphorylation mediated the promotive role of STX6 in HCC progression. ( A ) Protein levels and quantification results of total and phosphorylated STAT3 in control and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO. β-actin served as the loading control. ( B ) The representative images and quantification results of control and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO in colony formation assays. n = 3 per group. ( C ) The representative images and quantification results of control cells and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO in Transwell migration assays. n = 6 per group. ( D ) Protein levels and quantification results of PCNA, cyclin D1, E-cadherin and N-cadherin in control and Huh7 STX6-OE cells treated with p-STAT3 inhibitor or DMSO. n = 6 per group. β-actin served as the loading control. Data was given as mean ± SD. For statistical analysis, the two-tailed Student’s t -test was used in A - D . n.s. indicates no significant difference; * P < 0.05; ** P < 0.01

Techniques: Migration, Two Tailed Test

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1) Product Images from "miR ‐375‐3p/ STX6 Exacerbates Atherosclerosis by Promoting Endothelial Cell Senescence via Activation of TGF ‐Beta Signals"

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