sst5  (Biorbyt)

Journal: bioRxiv

Article Title: Somatostatin released by single O-LM interneurons slowly inhibits intrinsic neuronal excitability in a paracrine and autocrine manner

doi: 10.1101/2025.08.31.673310

Figure Lengend Snippet: A. Immunostaining of Chromagranin A and SST in an O-LM cell. Left, immunostaining of SST. Middle, immunostaining of chromagranin A. Right, merge. B. Immunostaining of SST1. C. Immunostaining of SST2. D. Immunonstaining of SST4. E. Immunostatining of SST5. Arrows indicate the co-localization of SST and chromagranin (A) or SST receptors (B-E).

Article Snippet: The following primary antibodies were used in these experiments: mouse anti-SST (1:500, Thermo Fisher Scientific 14-9751-82), mouse anti-Phosph-CaMKII alpha (Thr286; 1:100, Thermo Fisher Scientific MA1-047), rabbit anti-chromogranin A (1:200, Synaptic Systems 259 003), rabbit anti-SST1 (1:500, Thermo Fisher Scientific, PA3-206), rabbit anti-SST2, SST3, SST4 and SST5 (1:100, 1:200, 1: 400, 1:200, Alomone Labs, ASR-006, ASR-003, ASR-004 and ASR-005).

Techniques: Immunostaining

Journal: bioRxiv

Article Title: Somatostatin released by single O-LM interneurons slowly inhibits intrinsic neuronal excitability in a paracrine and autocrine manner

doi: 10.1101/2025.08.31.673310

Figure Lengend Snippet: Immunolabelling of phosphorylated α-CaMKII (left) and SST1 (A, middle), SST2 (B, middle), SST3 (C, middle), SST4 (D, middle) and SST5 (E, middle). Right, merge confocal images showing colocalization in the cell body of phosphorylated α-CaMKII and SST1 (A), SST2 (B), SST4 (D) and SST5 (E) but not with SST3 (C).

Article Snippet: The following primary antibodies were used in these experiments: mouse anti-SST (1:500, Thermo Fisher Scientific 14-9751-82), mouse anti-Phosph-CaMKII alpha (Thr286; 1:100, Thermo Fisher Scientific MA1-047), rabbit anti-chromogranin A (1:200, Synaptic Systems 259 003), rabbit anti-SST1 (1:500, Thermo Fisher Scientific, PA3-206), rabbit anti-SST2, SST3, SST4 and SST5 (1:100, 1:200, 1: 400, 1:200, Alomone Labs, ASR-006, ASR-003, ASR-004 and ASR-005).

Techniques:

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 mRNA expression in CRC patients and in histologically normal colorectal mucosa from the same patients (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal expression of SST and SST1-5 mRNA in colorectal cancer samples (CRC) and non-tumor samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Incidence of somatostatin (SST) and SST receptor (SST1-5) expression in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Detection of IHC expression pattern of SST receptors (SST1-5) in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 immunoexpression in colorectal cancer (CRC), lymph node metastasis (LNM), and control mucosa (C) (A%).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal immunoexpression of SST and SST1-5 in colorectal cancer samples (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 expression in relation to different IHC patterns of cellular localization.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and or SST1-5 (mRNA and peptide) in colorectal cancer (CRC) and control mucosa samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 immunoexpression in colorectal cancer (CRC) and control samples (C) in relation to different anatomical sites of the large intestine.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 mRNA expression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous subtypes of CRC.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 immunoexpression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous CRC subtypes.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and SST1-5 mRNA in colorectal cancer (CRC), control colorectal mucosa (C), age of the patients, and basic laboratory tests.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control

Journal: Life

Article Title: Pharmacological Characterization of Veldoreotide as a Somatostatin Receptor 4 Agonist

doi: 10.3390/life11101075

Figure Lengend Snippet: Cell proliferation in ( A ) wild-type BON-1 cells or BON-1 cells expressing SST4 receptor following treatment with ( B ) 10 µM SS-14 or ( C ) 10 µM veldoreotide for 24 h at 37 °C. In C, SST4 receptor agonist J-2156 was compared with SS-14 and veldoreotide. Antagonists to the SST2 receptor (BIM23454) and SST5 receptor (BIM23867) were used. Data shown were generated from three to four independent experiments performed in duplicate (mean ± SEM). Absorbance data for cells treated with agonists were normalized to vehicle (control). * p < 0.05 vs. control; ** p < 0.01 vs. control; *** p < 0.001. SEM = standard error of the mean.

Article Snippet: SST2 and SST5 receptor antagonists BIM23454 and BIM23867, respectively, were obtained from Ipsen Bioscience (Cambridge, MA, USA).

Techniques: Expressing, Generated, Control

Journal: Cancers

Article Title: gsp Mutation Is Not a Molecular Biomarker of Long-Term Response to First-Generation Somatostatin Receptor Ligands in Acromegaly

doi: 10.3390/cancers13194857

Figure Lengend Snippet: Representative images of immunohistochemical expression patterns of somatostatin receptors subtype 2 and 5 and cytokeratin. ( A ) micrograph showing membrane SST2 immunostaining in 100% of cells with high intensity (IRS score 12–high); ( B ) SST2 immunostaining in approximately 20% of cells with moderate intentisty (IRS 4–low); ( C ) SST5 immunostaining in 100% of cells with high intensity (IRS score 12–high); ( D ) SST5 immunostaining in less than 10% of cells with low intensity (IRS 1–low); ( E ) densely granulated tumor—inset shows a magnified view of the perinuclear staining; and ( F ) sparsely granulated tumor—inset shows a magnified view of the dot-like staining.

Article Snippet: Rabbit monoclonal antibodies directed against SST2 (UMB-1, 1:5000, Abcam, Cambridge, UK, Cat. No. ab134152), SST5 (UMB-4, 1:2000, Abcam, Cambridge, UK, Cat. No. ab109495) and cytokeratin (CAM5.2) (1:10,000, Cell Marque, Rocklin, CA, USA, Cat. No. 452M-95) were used.

Techniques: Immunohistochemical staining, Expressing, Membrane, Immunostaining, Staining

Journal: Cancers

Article Title: gsp Mutation Is Not a Molecular Biomarker of Long-Term Response to First-Generation Somatostatin Receptor Ligands in Acromegaly

doi: 10.3390/cancers13194857

Figure Lengend Snippet: gsp mutation and SST5 protein ( A ) and mRNA ( B ) expression. Three outliers from the gsp - group (666, 1075, and 1318 copies) and one from the gsp + group (214 copies were excluded from the graph for better visualization).

Article Snippet: Rabbit monoclonal antibodies directed against SST2 (UMB-1, 1:5000, Abcam, Cambridge, UK, Cat. No. ab134152), SST5 (UMB-4, 1:2000, Abcam, Cambridge, UK, Cat. No. ab109495) and cytokeratin (CAM5.2) (1:10,000, Cell Marque, Rocklin, CA, USA, Cat. No. 452M-95) were used.

Techniques: Mutagenesis, Expressing

Journal: Cancers

Article Title: gsp Mutation Is Not a Molecular Biomarker of Long-Term Response to First-Generation Somatostatin Receptor Ligands in Acromegaly

doi: 10.3390/cancers13194857

Figure Lengend Snippet: Comparison of demographic, laboratory, imaging, molecular, histopathological, and treatment response to first-generation somatostatin receptor ligands data between gsp+ and gsp- tumors.

Article Snippet: Rabbit monoclonal antibodies directed against SST2 (UMB-1, 1:5000, Abcam, Cambridge, UK, Cat. No. ab134152), SST5 (UMB-4, 1:2000, Abcam, Cambridge, UK, Cat. No. ab109495) and cytokeratin (CAM5.2) (1:10,000, Cell Marque, Rocklin, CA, USA, Cat. No. 452M-95) were used.

Techniques: Comparison, Imaging, Biomarker Discovery, Control