Journal: Frontiers in Microbiology
Article Title: In vitro study on anti- Helicobacter pylori effects of DL-3-n-butylphthalide-loaded silk fibroin nanoparticles
doi: 10.3389/fmicb.2025.1750216
Figure Lengend Snippet: Molecular mechanisms of drug action against H. pylori . (A) Impact of NBP and NBP-NPs treatment on virulence gene expression levels in ATCC 700392. (B) Impact of NBP and NBP-NPs treatment on virulence gene expression levels in SS1. (C–E) Effect of drug treatment on urease activity in ATCC 700392. (F–H) Effect of drug treatment on urease activity in SS1 ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control group). (I) Drug-induced ultrastructural alterations in H. pylori SS1 observed by SEM (30.0 kx, 40.0 kx, 70.0 kx). CagA protein expression in H. pylori SS1 treated with (J) NBP and (K) NBP-NPs ( n =3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. con trol group).
Article Snippet: The standard strain ATCC 700392 and the clinical strain SS1 were exposed to NBP and NBP-NPs solutions at concentrations of 2 × MIC, 1 × MIC, and 0.5 × MIC under microaerophilic conditions at 37 °C with shaking at 150 rpm for 3 days.
Techniques: Gene Expression, Activity Assay, Control, Expressing