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sr7826  (Tocris)


Bioz Verified Symbol Tocris is a verified supplier
Bioz Manufacturer Symbol Tocris manufactures this product  
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    Tocris sr7826
    Effect of two LIMK inhibitors, LIMKi3 and <t>SR7826,</t> on the ability of CCK-8 (0.3 and 100 nM) to alter activation of LIMK (A) and cofilin (B) . Isolated pancreatic acini were incubated in the absence or presence of LIMKi3 (10 µM) or SR7826 (10 µM) for 3 h and then incubated with no addition (control), CCK-8 (0.3 or 100 nM) for 3 min, and then lysed. Western blots were analyzed using anti-pT508 LIMK and anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors.
    Sr7826, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sr7826/product/Tocris
    Average 93 stars, based on 27 article reviews
    sr7826 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth"

    Article Title: Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2023.1147572

    Effect of two LIMK inhibitors, LIMKi3 and SR7826, on the ability of CCK-8 (0.3 and 100 nM) to alter activation of LIMK (A) and cofilin (B) . Isolated pancreatic acini were incubated in the absence or presence of LIMKi3 (10 µM) or SR7826 (10 µM) for 3 h and then incubated with no addition (control), CCK-8 (0.3 or 100 nM) for 3 min, and then lysed. Western blots were analyzed using anti-pT508 LIMK and anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors.
    Figure Legend Snippet: Effect of two LIMK inhibitors, LIMKi3 and SR7826, on the ability of CCK-8 (0.3 and 100 nM) to alter activation of LIMK (A) and cofilin (B) . Isolated pancreatic acini were incubated in the absence or presence of LIMKi3 (10 µM) or SR7826 (10 µM) for 3 h and then incubated with no addition (control), CCK-8 (0.3 or 100 nM) for 3 min, and then lysed. Western blots were analyzed using anti-pT508 LIMK and anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors.

    Techniques Used: CCK-8 Assay, Activation Assay, Isolation, Incubation, Control, Western Blot



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    Effect of two LIMK inhibitors, LIMKi3 and <t>SR7826,</t> on the ability of CCK-8 (0.3 and 100 nM) to alter activation of LIMK (A) and cofilin (B) . Isolated pancreatic acini were incubated in the absence or presence of LIMKi3 (10 µM) or SR7826 (10 µM) for 3 h and then incubated with no addition (control), CCK-8 (0.3 or 100 nM) for 3 min, and then lysed. Western blots were analyzed using anti-pT508 LIMK and anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors.
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    Effect of two LIMK inhibitors, LIMKi3 and <t>SR7826,</t> on the ability of CCK-8 (0.3 and 100 nM) to alter activation of LIMK (A) and cofilin (B) . Isolated pancreatic acini were incubated in the absence or presence of LIMKi3 (10 µM) or SR7826 (10 µM) for 3 h and then incubated with no addition (control), CCK-8 (0.3 or 100 nM) for 3 min, and then lysed. Western blots were analyzed using anti-pT508 LIMK and anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors.
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    Image Search Results


    Effect of two LIMK inhibitors, LIMKi3 and SR7826, on the ability of CCK-8 (0.3 and 100 nM) to alter activation of LIMK (A) and cofilin (B) . Isolated pancreatic acini were incubated in the absence or presence of LIMKi3 (10 µM) or SR7826 (10 µM) for 3 h and then incubated with no addition (control), CCK-8 (0.3 or 100 nM) for 3 min, and then lysed. Western blots were analyzed using anti-pT508 LIMK and anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors.

    Journal: Frontiers in Physiology

    Article Title: Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth

    doi: 10.3389/fphys.2023.1147572

    Figure Lengend Snippet: Effect of two LIMK inhibitors, LIMKi3 and SR7826, on the ability of CCK-8 (0.3 and 100 nM) to alter activation of LIMK (A) and cofilin (B) . Isolated pancreatic acini were incubated in the absence or presence of LIMKi3 (10 µM) or SR7826 (10 µM) for 3 h and then incubated with no addition (control), CCK-8 (0.3 or 100 nM) for 3 min, and then lysed. Western blots were analyzed using anti-pT508 LIMK and anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors.

    Article Snippet: LIMKi3 and SR7826 were from TOCRIS bioscience (Bristol, UK).

    Techniques: CCK-8 Assay, Activation Assay, Isolation, Incubation, Control, Western Blot