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Biacore surface plasmon resonance spr analysis
Surface Plasmon Resonance Spr Analysis, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spr+analysis/plasmon+resonance+surface/pm42304484-176-19-29
Average 86 stars, based on 1 article reviews
surface plasmon resonance spr analysis - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

SPR Assay:

Article Title: Bruceine A protects nuclear receptor 4A1 from ubiquitin-degradation to alleviate mesangial proliferative glomerulonephritis
Article Snippet: .. SPR analysis (BIAcore 1 K, CM5 chip) quantified BA binding kinetics to wild-type/mutant NR4A1/LBD at 25 °C. ..

Article Title: A new approach to discovering a broad-spectrum anti-respiratory virus drug: Tubeimoside II modulates host factor PACT to potentiate the RIG-I antiviral pathway
Article Snippet: .. SPR analysis The binding assay was was conducted on a BIAcore T200 (25 °C) with CM5 chips. ..

Article Title: Antigen-binding domain
Article Snippet: .. SPR analysis (Biacore) showed that the antibodies bound with an affinity in the nM range and the selected antibodies bind to PSMA (human and Rhesus) in IHC staining. ..

Article Title: Huaier-Derived Arnicolide D Induces Ferroptosis in Gastric Cancer via SRC/TNF-Mediated GPX4 Inactivation
Article Snippet: Background: While ferroptosis induction has emerged as a promising strategy against gastric cancer (GC), clinically viable natural inducers remain scarce.. Huaier n-butanol extract (HBE), a crude extract derived from the traditional Chinese medicinal fungus Huaier (Trametes robiniophila Murr.), exhibits promising anticancer activity, although its bioactive constituents and underlying mechanisms remain to be fully elucidated.. Purpose: This study aimed to investigate HBE's anti-GC effects and identify its bioactive components, with a focus on elucidating the ferroptosis-related mechanisms underlying their therapeutic actions.

Article Title: Compounds and methods targeting epiregulin
Article Snippet: .. SPR Analysis of Antibody 1 Fab Fragment Binding to Mono Fc Human EREG Mutants A Biacore 8K instrument and reagents (Cytiva) were used for the SPR analysis of Antibody 1 Fab fragment binding to both mono Fc human EREG mutants. .. Low levels of mono Fc human EREG mutants were immobilized in the sample flow cells (Fc2) of a CM5 S series sensor chip (Cytiva P/N BR100530) using an amine coupling kit (Cytiva P/N BR 100050) and the “Low Level CM5 Coupling” method in Biacore 8K control software.

Article Title: Generation Using Phage-Display of pH-Dependent Antibodies Against the Tumor-Associated Antigen AXL
Article Snippet: The 6His tag was detected using a mouse anti-polyhistidine monoclonal antibody (Clone # AD1.1.10R, MAB050, R&D), followed by A488-labeled goat anti-murine Fab (AB_2536161, A28175, ThermoFisher Scientific, Waltham, MA, USA). .. SPR analysis was performed with BIAcore and human AXL-ECD-Fc (R&D SystemsBio-Techne, Minneapolis, MN, USA) covalently immobilized on a CM5 chip at pH 4 (Amine coupling kit, Cytiva). ..

Article Title: A 3,4-diamino-substituted coumarin derivative as a TRBP inhibitor suppresses tumor growth in breast cancer by inducing cellular senescence
Article Snippet: .. Complementary validation through SDS-PAGE analysis with Coomassie staining confirmed the compound’s dose-dependent stabilization of TRBP in the lysate system. (E) MCF-7 cells were treated with 3ai (1, 10, 25 μM) for 24 h, and the mRNA expression levels of TRBP were measured by qRT-PCR. (F) SPR analysis using Biacore CM5 chip with recombinant TRBP protein immobilized to detect the binding of 3ai to TRBP protein. ..

Binding Assay:

Article Title: Bruceine A protects nuclear receptor 4A1 from ubiquitin-degradation to alleviate mesangial proliferative glomerulonephritis
Article Snippet: .. SPR analysis (BIAcore 1 K, CM5 chip) quantified BA binding kinetics to wild-type/mutant NR4A1/LBD at 25 °C. ..

Article Title: A new approach to discovering a broad-spectrum anti-respiratory virus drug: Tubeimoside II modulates host factor PACT to potentiate the RIG-I antiviral pathway
Article Snippet: .. SPR analysis The binding assay was was conducted on a BIAcore T200 (25 °C) with CM5 chips. ..

Article Title: Compounds and methods targeting epiregulin
Article Snippet: .. SPR Analysis of Antibody 1 Fab Fragment Binding to Mono Fc Human EREG Mutants A Biacore 8K instrument and reagents (Cytiva) were used for the SPR analysis of Antibody 1 Fab fragment binding to both mono Fc human EREG mutants. .. Low levels of mono Fc human EREG mutants were immobilized in the sample flow cells (Fc2) of a CM5 S series sensor chip (Cytiva P/N BR100530) using an amine coupling kit (Cytiva P/N BR 100050) and the “Low Level CM5 Coupling” method in Biacore 8K control software.

Article Title: A 3,4-diamino-substituted coumarin derivative as a TRBP inhibitor suppresses tumor growth in breast cancer by inducing cellular senescence
Article Snippet: .. Complementary validation through SDS-PAGE analysis with Coomassie staining confirmed the compound’s dose-dependent stabilization of TRBP in the lysate system. (E) MCF-7 cells were treated with 3ai (1, 10, 25 μM) for 24 h, and the mRNA expression levels of TRBP were measured by qRT-PCR. (F) SPR analysis using Biacore CM5 chip with recombinant TRBP protein immobilized to detect the binding of 3ai to TRBP protein. ..

Analysis:

Article Title: A new approach to discovering a broad-spectrum anti-respiratory virus drug: Tubeimoside II modulates host factor PACT to potentiate the RIG-I antiviral pathway
Article Snippet: .. SPR analysis The binding assay was was conducted on a BIAcore T200 (25 °C) with CM5 chips. ..

Immunohistochemistry:

Article Title: Antigen-binding domain
Article Snippet: .. SPR analysis (Biacore) showed that the antibodies bound with an affinity in the nM range and the selected antibodies bind to PSMA (human and Rhesus) in IHC staining. ..

Staining:

Article Title: Antigen-binding domain
Article Snippet: .. SPR analysis (Biacore) showed that the antibodies bound with an affinity in the nM range and the selected antibodies bind to PSMA (human and Rhesus) in IHC staining. ..

Article Title: A 3,4-diamino-substituted coumarin derivative as a TRBP inhibitor suppresses tumor growth in breast cancer by inducing cellular senescence
Article Snippet: .. Complementary validation through SDS-PAGE analysis with Coomassie staining confirmed the compound’s dose-dependent stabilization of TRBP in the lysate system. (E) MCF-7 cells were treated with 3ai (1, 10, 25 μM) for 24 h, and the mRNA expression levels of TRBP were measured by qRT-PCR. (F) SPR analysis using Biacore CM5 chip with recombinant TRBP protein immobilized to detect the binding of 3ai to TRBP protein. ..

Biomarker Discovery:

Article Title: A 3,4-diamino-substituted coumarin derivative as a TRBP inhibitor suppresses tumor growth in breast cancer by inducing cellular senescence
Article Snippet: .. Complementary validation through SDS-PAGE analysis with Coomassie staining confirmed the compound’s dose-dependent stabilization of TRBP in the lysate system. (E) MCF-7 cells were treated with 3ai (1, 10, 25 μM) for 24 h, and the mRNA expression levels of TRBP were measured by qRT-PCR. (F) SPR analysis using Biacore CM5 chip with recombinant TRBP protein immobilized to detect the binding of 3ai to TRBP protein. ..

SDS Page:

Article Title: A 3,4-diamino-substituted coumarin derivative as a TRBP inhibitor suppresses tumor growth in breast cancer by inducing cellular senescence
Article Snippet: .. Complementary validation through SDS-PAGE analysis with Coomassie staining confirmed the compound’s dose-dependent stabilization of TRBP in the lysate system. (E) MCF-7 cells were treated with 3ai (1, 10, 25 μM) for 24 h, and the mRNA expression levels of TRBP were measured by qRT-PCR. (F) SPR analysis using Biacore CM5 chip with recombinant TRBP protein immobilized to detect the binding of 3ai to TRBP protein. ..

Expressing:

Article Title: A 3,4-diamino-substituted coumarin derivative as a TRBP inhibitor suppresses tumor growth in breast cancer by inducing cellular senescence
Article Snippet: .. Complementary validation through SDS-PAGE analysis with Coomassie staining confirmed the compound’s dose-dependent stabilization of TRBP in the lysate system. (E) MCF-7 cells were treated with 3ai (1, 10, 25 μM) for 24 h, and the mRNA expression levels of TRBP were measured by qRT-PCR. (F) SPR analysis using Biacore CM5 chip with recombinant TRBP protein immobilized to detect the binding of 3ai to TRBP protein. ..

Recombinant:

Article Title: A 3,4-diamino-substituted coumarin derivative as a TRBP inhibitor suppresses tumor growth in breast cancer by inducing cellular senescence
Article Snippet: .. Complementary validation through SDS-PAGE analysis with Coomassie staining confirmed the compound’s dose-dependent stabilization of TRBP in the lysate system. (E) MCF-7 cells were treated with 3ai (1, 10, 25 μM) for 24 h, and the mRNA expression levels of TRBP were measured by qRT-PCR. (F) SPR analysis using Biacore CM5 chip with recombinant TRBP protein immobilized to detect the binding of 3ai to TRBP protein. ..



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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Image Search Results


Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore T200. (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.

Journal: Advanced Science

Article Title: Mitochondrial Localization of Cx26 Promotes Colorectal Cancer Malignancy and Exposes a Fatal Vulnerability to Drug Targeting

doi: 10.1002/advs.78117

Figure Lengend Snippet: Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore T200. (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.

Article Snippet: The Biacore T200 molecular interaction analysis system (GE Healthcare, Uppsala, Sweden) was used to measure the change in response value when the gradient concentration of BTZ solution flowed through the chip, and the kinetic constant of the interaction between small molecules and proteins was obtained.

Techniques: Expressing, Western Blot, Control, MTT Assay, Docking Assay, Analysis, Incubation, Binding Assay, Activity Assay, shRNA