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Plexera Inc spr data analysis module software
Spr Data Analysis Module Software, supplied by Plexera Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spr+analysis/analysis+data+module/us12656342-506-14-13
Average 86 stars, based on 1 article reviews
spr data analysis module software - by Bioz Stars, 2026-10
86/100 stars

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Binding Assay:

Article Title: FASLG Derived from Fibroblasts in Hydroxyapatite-Rich Microenvironment Induces Urothelial Anoikis to Trigger Randall's Plaque Exposure.
Article Snippet: .. Subsequently, the regeneration buffer as flowed at 2 μL min− 1 for 200 s. All binding parameters ere recorded and processed using the manufacturer’s SPR data nalysis platform (Plexera SPR Data Analysis Module). ..

Article Title: Linoleic acid binds to SARS-CoV-2 RdRp and represses replication of seasonal human coronavirus OC43.
Article Snippet: .. Real-time binding signals were recorded and analyzed by Data Analysis Module (DAM, Plexera Bioscience, Seattle, WA). .. Kinetic analysis was performed using BIA evaluation 4.1 software (Biacore, Inc.).

Article Title: Modular Assembly of Lipid Nanoparticles for Targeted mRNA Therapeutics and Vaccines.
Article Snippet: Targeted mRNA delivery remains a key challenge for lipid nanoparticles (LNPs), as existing surface functionalization strategies often suffer from uncontrolled cross-linking, aggregation, and immunogenicity.. Conventional tetrameric streptavidin-biotin coupling, while biochemically robust, has limited translational potential due to its multivalency and poor structural control.. Here, a monomeric streptavidin (mSA)-based modular assembly platform is presented that enables rapid, stable, and customizable functionalization of LNPs.

Article Title: (+)-JJ-74-138 is a novel non-competitive androgen receptor antagonist
Article Snippet: The SPRi measurements after binding and washing were recorded in AU (Normalized reflection unit) and performed with PlexArray HT (Plexera Bioscience, Seattle, WA,). .. The binding signals were analyzed by Data Analysis Module (DAM, Plexera Bioscience, Seattle, WA,) and the average reflectivity variations were plotted as a function of time with GraphPad Prism. .. The kinetic parameters of binding were performed with BIAevaluation 4.1 software (Biacore, Inc.).

SPR Assay:

Article Title: FASLG Derived from Fibroblasts in Hydroxyapatite-Rich Microenvironment Induces Urothelial Anoikis to Trigger Randall's Plaque Exposure.
Article Snippet: .. Subsequently, the regeneration buffer as flowed at 2 μL min− 1 for 200 s. All binding parameters ere recorded and processed using the manufacturer’s SPR data nalysis platform (Plexera SPR Data Analysis Module). ..

Article Title: Adaptation of NAPPA for surface plasmon resonance imaging analyses
Article Snippet: .. Regions-of-interest in the AVI video format were first identified and analyzed with the Plexera SPR Data Analysis Module software. ..

Article Title: Isoalantolactone exerts anti-melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models.
Article Snippet: .. All data were generated and analyzed using Plexera SPR Data analysis Module software (Plexera, USA). ..

Software:

Article Title: Adaptation of NAPPA for surface plasmon resonance imaging analyses
Article Snippet: .. Regions-of-interest in the AVI video format were first identified and analyzed with the Plexera SPR Data Analysis Module software. ..

Article Title: Adaptation of NAPPA for surface plasmon resonance imaging analyses
Article Snippet: .. Spot numbers refer to the spot location used from the Plexera Data Module software files. ..

Article Title: Isoalantolactone exerts anti-melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models.
Article Snippet: .. All data were generated and analyzed using Plexera SPR Data analysis Module software (Plexera, USA). ..

Article Title: Modular Assembly of Lipid Nanoparticles for Targeted mRNA Therapeutics and Vaccines.
Article Snippet: Targeted mRNA delivery remains a key challenge for lipid nanoparticles (LNPs), as existing surface functionalization strategies often suffer from uncontrolled cross-linking, aggregation, and immunogenicity.. Conventional tetrameric streptavidin-biotin coupling, while biochemically robust, has limited translational potential due to its multivalency and poor structural control.. Here, a monomeric streptavidin (mSA)-based modular assembly platform is presented that enables rapid, stable, and customizable functionalization of LNPs.

Article Title: A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism
Article Snippet: .. For data analysis, we choose two software packages, ORIGIN Lab and Data Analysis Module (DAM) of Plexera. ..

Generated:

Article Title: Isoalantolactone exerts anti-melanoma effects via inhibiting PI3K/AKT/mTOR and STAT3 signaling in cell and mouse models.
Article Snippet: .. All data were generated and analyzed using Plexera SPR Data analysis Module software (Plexera, USA). ..

Titration:

Article Title: Modular Assembly of Lipid Nanoparticles for Targeted mRNA Therapeutics and Vaccines.
Article Snippet: Targeted mRNA delivery remains a key challenge for lipid nanoparticles (LNPs), as existing surface functionalization strategies often suffer from uncontrolled cross-linking, aggregation, and immunogenicity.. Conventional tetrameric streptavidin-biotin coupling, while biochemically robust, has limited translational potential due to its multivalency and poor structural control.. Here, a monomeric streptavidin (mSA)-based modular assembly platform is presented that enables rapid, stable, and customizable functionalization of LNPs.



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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore <t>T200.</t> (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.
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Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore T200. (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.

Journal: Advanced Science

Article Title: Mitochondrial Localization of Cx26 Promotes Colorectal Cancer Malignancy and Exposes a Fatal Vulnerability to Drug Targeting

doi: 10.1002/advs.78117

Figure Lengend Snippet: Highly malignant CRC is more sensitive to bortezomib. (A) The relation between GJB2 expression and IC 50 values of BTZ in CRC cells was analyzed by GDSC. (B) The protein levels of Cx26 and Tubulin in SW480‐NC and SW480‐OE‐Cx26 cells were detected by western blot. Tubulin was used as a loading control. The IC 50 values of BTZ in SW480‐NC and SW480‐OE‐Cx26 cells were measured by MTT assay. *** p < 0.001 vs . Control; by unpaired student's t ‐test (mean ± SEM). (C) Molecular docking analysis of BTZ and PIMT via Discovery Studio. On the right is the 2D diagram of the interaction between BTZ and amino acid residues of PIMT. (D) PIMT was incubated with DMSO, BTZ (0.01, 0.1, 1 µM), and SAH (1 µM), respectively, delta Tm was calculated compared to the DMSO group. Data are presented as mean ± SEM of at least three replicates. (E) Dose‐response curves for a gradient dilution of BTZ binding to PIMT were measured by Biacore T200. (F) PIMT activity was determined using a methyltransferase activity assay kit. ** p < 0.01 vs . DMSO; by one‐way ANOVA followed by Dunnett's multiple comparisons test (mean ± SEM). (G) The IC 50 values of BTZ in SW620‐shRNA‐NC and SW620‐shRNA‐PIMT cells were measured by MTT assay. Data are presented as the mean ± SEM of three replicates.

Article Snippet: The Biacore T200 molecular interaction analysis system (GE Healthcare, Uppsala, Sweden) was used to measure the change in response value when the gradient concentration of BTZ solution flowed through the chip, and the kinetic constant of the interaction between small molecules and proteins was obtained.

Techniques: Expressing, Western Blot, Control, MTT Assay, Docking Assay, Analysis, Incubation, Binding Assay, Activity Assay, shRNA