spa17 rabbit polyclonal antibody  (Proteintech)

Journal: Asian Journal of Andrology

Article Title: Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

doi: 10.4103/aja202329

Figure Lengend Snippet: The DNA sequences of target gene

Article Snippet: SPA17 rabbit polyclonal antibody, phosphorylated protein kinase B (p-AKT) rabbit polyclonal antibody, and AKT rabbit polyclonal antibody were purchased from Proteintech (Rosemont, CA, USA).

Techniques:

Journal: Asian Journal of Andrology

Article Title: Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

doi: 10.4103/aja202329

Figure Lengend Snippet: Relative expression of HSPA2, PCNA, SPA17 protein in the L-DFI and H-DFI groups. ( a ) Western blot analysis and ( b ) densitometric ratio histogram of HSPA2, PCNA and SPA17 with 4 semen samples of L-DFI (L-1 to L-4) and H-DFI (H-1 to H-4) groups each. Western blot analysis of SPA17 with 4 semen samples of L-DFI and H-DFI groups each, ( c ) L-9 to L-12, and H-9 to H-12, and ( d ) L-5 to L-8, and H-5 to H-8 in the L-DFI and H-DFI groups. We selected 12 semen samples in the L-DFI (L-1 to L-12) and H-DFI (H-1 to H-12) groups each. ** P < 0.01. Data is presented as mean ± standard deviation. HSPA2: heat shock protein A2; PCNA: proliferating cell nuclear antigen; SPA17: sperm protein antigen 17; H-DFI: high sperm DNA fragmentation index; L-DFI: low sperm DNA fragmentation index; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: SPA17 rabbit polyclonal antibody, phosphorylated protein kinase B (p-AKT) rabbit polyclonal antibody, and AKT rabbit polyclonal antibody were purchased from Proteintech (Rosemont, CA, USA).

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Asian Journal of Andrology

Article Title: Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

doi: 10.4103/aja202329

Figure Lengend Snippet: Relative expressions of PKA-associated protein in L-DFI and H-DFI groups. Western blot analysis of PKA-associated protein ( a ) with 3 semen samples of L-DFI (L-13 to L-15) and H-DFI (H-13 to H-15) groups, respectively, and ( b ) with 4 semen samples of L-DFI (L-15 to L-19) and H-DFI (H-15 to H-19) groups, respectively. Histograms demonstrated the densitometric ratio of ( c ) PKARII, ( d ) AKAP4, ( e ) SPA17, ( f ) PI3K, ( g ) p-AKT, ( h ) AKT, and ( i ) p-ERK in L-DFI and H-DFI groups. We selected 7 semen samples in the L-DFI (L-13 to L-19) and H-DFI (H-13 to H-19) groups, respectively. * P < 0.05. Data are presented as mean ± standard deviation. PKA: protein kinase A; AKAP: a-kinase anchoring proteins; PI3K: phosphoInositide-3 kinase; AKT: protein kinase B; p-AKT: phosphorylated protein kinase B; p-ERK: extracellular signal-regulated kinase of phosphorylation; H-DFI: high sperm DNA fragmentation index; L-DFI: low sperm DNA fragmentation index; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: SPA17 rabbit polyclonal antibody, phosphorylated protein kinase B (p-AKT) rabbit polyclonal antibody, and AKT rabbit polyclonal antibody were purchased from Proteintech (Rosemont, CA, USA).

Techniques: Western Blot, Standard Deviation, Phospho-proteomics

Journal: Asian Journal of Andrology

Article Title: Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

doi: 10.4103/aja202329

Figure Lengend Snippet: The protein expression of SPA17, AKAP4 and PKARII after H 2 O 2 treatment of semen from the L-DFI group. Western blot analysis of SPA17, AKAP4 and PKARII ( a ) after 20 µmol l −1 and 40 µmol l −1 H 2 O 2 treatment, and ( b ) after 40 µmol l −1 and 80 µmol l −1 H 2 O 2 treatment. Histograms demonstrated the densitometric ratio of ( c ) AKAP4, ( d ) PKARII, and ( e ) SPA17. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are presented as mean ± standard deviation. SPA17: sperm protein antigen 17; PKA: protein kinase A; AKAP: A-kinase anchoring proteins; L-DFI: low sperm DNA fragmentation index; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: SPA17 rabbit polyclonal antibody, phosphorylated protein kinase B (p-AKT) rabbit polyclonal antibody, and AKT rabbit polyclonal antibody were purchased from Proteintech (Rosemont, CA, USA).

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: Basic information of SPA17. (A) The level of SPA17 expression between tumor and normal tissues in each type of cancer based on the integrated data from TCGA and GTEx datasets. (B) Analysis of SPA17 change frequency in pan-cancer research according to cBioPortal database. (C) The SPA17 expression levels between SPA17-deletion and diploid TGCT patients. (D) The immunofluorescence images of SPA17 protein, nucleus, endoplasmic reticulum (ER), microtubules and the incorporative images in A-431 and U251 cell lines. (E) The protein-protein interaction (PPI) network presents the proteins interacting with SPA17. (F, G) Western blot protein detection of the SPA17 expression levels in paired GBM and adjacent normal tissues. The labelled asterisk indicated the statistical p-value (ns, p > 0.05, *p < 0.05, **p < 0.01 and ***p < 0.001).

Article Snippet: The Human Protein Atlas (HPA) and ComPPI websites were used to mine the SPA17 protein information.

Techniques: Expressing, Immunofluorescence, Western Blot

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: (A) Based on the univariate Cox regression and Kaplan-Meier models, summary of the correlation between SPA17 expression and overall survival (OS), disease-specific survival (DSS), disease-free interval (DFI) and progression-free interval (PFI). Red indicates that SPA17 is a risk factor for the prognosis of cancer patients, while blue represents a protective factor. Only p-values < 0.05 are shown. (B) The forest plot showed the prognostic role of SPA17 in cancers by univariate Cox regression. The red type of cancer represents SPA17 as a statistically significant risk factor, and blue represents a protective factor. (C–F) Kaplan-Meier overall survival curves of SPA17 in BRCA (C) , KIRC (D) , LGG (E) and TGCT (F) .

Article Snippet: The Human Protein Atlas (HPA) and ComPPI websites were used to mine the SPA17 protein information.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: The hallmarks gene set enrichment analysis (GSEA) of SPA17 in pan-cancer. The size of the circle represents the false discovery rate (FDR) value of each cancer enrichment item, and the color represents the normalized enrichment score (NES) of each enrichment item.

Article Snippet: The Human Protein Atlas (HPA) and ComPPI websites were used to mine the SPA17 protein information.

Techniques:

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: The correlations of SPA17 expression and the infiltration levels of CD4+ T cells, CAF, progenitor, Endo, Eos, HSC, Tfh, γδT, NKT, regulatory T cells (Tregs), B cells, neutrophils, monocytes, macrophages, dendritic cells, NK cells, Mast cells and CD8+ T cells in cancers. Positive correlation in red and negative correlation in blue.

Article Snippet: The Human Protein Atlas (HPA) and ComPPI websites were used to mine the SPA17 protein information.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis

doi: 10.3389/fimmu.2022.844736

Figure Lengend Snippet: (A) The Spearman correlation heatmap shows the correlation between the expression of SPA17 and 47 kinds of immune regulators in pan-cancer. Red represents positive correlation and blue represents negative correlation. (B) Correlations between SPA17 expression and tumor mutation burden in pan-cancer. (C) Correlations between SPA17 expression and microsatellite instability in pan-cancer. (D) Kaplan-Meier curve of low and high-SPA17 subgroup in IMvigor210 cohort (anti-PD-L1), and the proportion of tumors (including kidney cancer) in the low-SPA17 and high-SPA17 subgroups in the IMvigor210 cohort who responded to PD-1 therapy. (E) Kaplan-Meier curve of GSE91061 (anti-PD-L1 melanoma) in the low and high-SPA17 patient groups, and the proportion of melanoma patients in the GSE91061 low and high-SPA17 subgroups that responded to anti-PD-1 therapy. The labelled asterisk indicated the statistical p-value (*p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: The Human Protein Atlas (HPA) and ComPPI websites were used to mine the SPA17 protein information.

Techniques: Expressing, Mutagenesis