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sp2 (ATCC)

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ATCC sp2
Sp2, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 755 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sp2/Sp2%2F0-Ag14/us12655206-413-56-71
Average 96 stars, based on 755 article reviews

sp2 - by Bioz Stars, 2026-10

96/100 stars

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Expressing:

Article Title: Method of treating psoriatic arthritis patients with inadequate response to TNF therapy with anti-IL23 specific antibody
Article Snippet: .. A number of suitable host cell lines capable of expressing intact glycosylated proteins have been developed in the art, and include the COS-1 (e.g., ATCC CRL 1650), COS-7 (e.g., ATCC CRL-1651), HEK293, BHK21 (e.g., ATCC CRL-10), CHO (e.g., ATCC CRL 1610) and BSC-1 (e.g., ATCC CRL-26) cell lines, Cos-7 cells, CHO cells, hep G2 cells, P3X63Ag8.653, SP2/0-Ag14, 293 cells, HeLa cells and the like, which are readily available from, for example, American Type Culture Collection, Manassas, Va. (www.atcc.org). ..

Isolation:

Article Title: Methods for diagnosing cancer using anti-BAG2 antibody
Article Snippet: .. B lymphocytes were isolated from the spleen, followed by fusion with the myeloma cells cultured with the isolated B lymphocytes, that is, the SP2/0-Ag14 cell line of ATCC, thereby obtaining fused cells. ..

Cell Culture:

Single Cell:

Article Title: Antibodies to CD40 with enhanced agonist activity
Article Snippet: .. For example, single cell suspensions of splenic lymphocytes from immunized mice can be fused to Sp2/0 nonsecreting mouse myeloma cells (ATCC, CRL 1581) with 50% PEG. .. Cells are plated at approximately 2×10′ in flat bottom microtiter plate, followed by a two week incubation in selective medium containing 10% fetal Clone Serum, 18% “653” conditioned media, 5% origen (IGEN), 4 mM L-glutamine, 1 mM sodium pyruvate, 5 mM HEPES, 0.055 mM 2-mercaptoethanol, 50 units/ml penicillin, 50 mg/ml streptomycin, 50 mg/ml gentamycin and 1×HAT (Sigma).

Generated:

Article Title: Discovery, Structural Characterization, and Preclinical Evaluation of Monoclonal Antibodies against Xylazine Poisoning
Article Snippet: Xylazine is a nonopioid sedative that has emerged as a major adulterant in the illicit drug supply, contributing to rising fatal overdoses across the United States.. Xylazine toxicity is not reversed by naloxone, and there are no current FDA-approved therapeutics to counteract its effects in humans.. To address this issue, we developed and characterized monoclonal antibodies (mAbs) capable of sequestering xylazine to prevent or treat acute toxicity.