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sox9  (Proteintech)


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    Structured Review

    Proteintech sox9
    Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of COL2A1, <t>SOX9,</t> MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
    Sox9, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sox9/SOX9+Antibody/pmc12963903-110-9-10
    Average 96 stars, based on 211 article reviews
    sox9 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration"

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.051

    Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of COL2A1, SOX9, MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
    Figure Legend Snippet: Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of COL2A1, SOX9, MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Techniques Used: Immunofluorescence, Expressing

    Expression analysis of cartilage-related proteins after treatment with AdHy@Pae. (A) Representative Western blot images showing ECM synthesis–related proteins (COL2A1, ACAN, COMP, and SOX9) and antioxidant markers (NRF2 and HO-1) in different groups. (B) Quantitative analysis of matrix-degrading enzymes (MMP13, ADAMTS5, ADAMTS1) and apoptosis-related proteins (BCL-2, Bax, and Caspase-3). Data are presented as mean ± SD (n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
    Figure Legend Snippet: Expression analysis of cartilage-related proteins after treatment with AdHy@Pae. (A) Representative Western blot images showing ECM synthesis–related proteins (COL2A1, ACAN, COMP, and SOX9) and antioxidant markers (NRF2 and HO-1) in different groups. (B) Quantitative analysis of matrix-degrading enzymes (MMP13, ADAMTS5, ADAMTS1) and apoptosis-related proteins (BCL-2, Bax, and Caspase-3). Data are presented as mean ± SD (n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Techniques Used: Expressing, Western Blot

    In vivo validation of the chondroprotective and mitochondrial regulatory effects of AdHy@Pae after therapy for 4 weeks. (A) Representative immunohistochemical staining of cartilage sections for COL2A1, SOX9, MMP3, and ADAMTS1 in different groups (Sham, PBS, Hy, AdHy, and AdHy@Pae). (B) Quantitative analysis of the immunohistochemical staining intensity showing relative expression levels of anabolic (COL2A1, SOX9) and catabolic (MMP3, ADAMTS1) markers. (C) TEM images of chondrocytes showing mitochondrial ultrastructure in each group, with high-magnification views (bottom panels) indicating cristae integrity and membrane morphology. Data are presented as mean ± SD (n = 3); ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗P < 0.0001.
    Figure Legend Snippet: In vivo validation of the chondroprotective and mitochondrial regulatory effects of AdHy@Pae after therapy for 4 weeks. (A) Representative immunohistochemical staining of cartilage sections for COL2A1, SOX9, MMP3, and ADAMTS1 in different groups (Sham, PBS, Hy, AdHy, and AdHy@Pae). (B) Quantitative analysis of the immunohistochemical staining intensity showing relative expression levels of anabolic (COL2A1, SOX9) and catabolic (MMP3, ADAMTS1) markers. (C) TEM images of chondrocytes showing mitochondrial ultrastructure in each group, with high-magnification views (bottom panels) indicating cristae integrity and membrane morphology. Data are presented as mean ± SD (n = 3); ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗P < 0.0001.

    Techniques Used: In Vivo, Biomarker Discovery, Immunohistochemical staining, Staining, Expressing, Membrane

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    Immunohistochemistry:

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    Article Title: Injectable self-healing hydrogel for co-delivery of stem cells and growth factors in intervertebral disc regeneration
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    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection-Driven cartilage regeneration.
    Article Snippet: Hematoxylin and eosin (H&E) (Servicebio, G1076), Safranin O–Fast Green (SO-FG) (Servicebio, G1053), and Toluidine Blue (TB) (Servicebio, G1032) staining evaluated cartilage morphology and matrix preservation, with histological grading performed using OARSI and Mankin scores. .. For immunohistochemistry, primary antibodies were COL2A1 (HUABIO, HA722733, 1:1000), SOX9 (Proteintech, 68350-1-Ig, 1:500), MMP3 (Servicebio, GB11132, 1:500), and ADAMTS1 (Affinity, DF13268, 1:100); sections were incubated with HRP-conjugated polymer secondary antibodies (Affinity, S0001), developed with DAB (Servicebio, G1212), and counterstained with hematoxylin. .. Whole-slide images were acquired on a digital scanner (Pannoramic MIDI, 3DHISTECH, Hungary), and quantification was performed in ImageJ.

    Incubation:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Hematoxylin and eosin (H&E) (Servicebio, G1076), Safranin O–Fast Green (SO-FG) (Servicebio, G1053), and Toluidine Blue (TB) (Servicebio, G1032) staining evaluated cartilage morphology and matrix preservation, with histological grading performed using OARSI and Mankin scores. .. For immunohistochemistry, primary antibodies were COL2A1 (HUABIO, HA722733, 1:1000), SOX9 (Proteintech, 68350-1-Ig, 1:500), MMP3 (Servicebio, GB11132, 1:500), and ADAMTS1 (Affinity, DF13268, 1:100); sections were incubated with HRP-conjugated polymer secondary antibodies (Affinity, S0001), developed with DAB (Servicebio, G1212), and counterstained with hematoxylin. .. Whole-slide images were acquired on a digital scanner (Pannoramic MIDI, 3DHISTECH, Hungary), and quantification was performed in ImageJ.

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection-Driven cartilage regeneration.
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    Article Title: Bioinspired scaffold recapitulating chondrogenic ontogeny and microenvironment for functional cartilage regeneration
    Article Snippet: .. After blocking the membranes for an hour at room temperature using fast blocking solution, the membranes were incubated with primary antibodies specific for SOX9 (1:2000, 67439-1-Ig, Proteintech), COL2 (1:200, NBP1-91056, Novus Biologicals), COL10 (1:500, 26984-1-AP, Proteintech), COL1 (1:2000, 67288-1-Ig, Proteintech), RUNX2 (1:200, 20700-1-AP, Proteintech), FGF2 (1:500, 11234-1-AP, Proteintech), FGF18 (1:500, 60341-1-Ig, Proteintech) and GAPDH (1:50000, 60004-1-Ig, Proteintech) for an entire night at 4 °C. ..

    Article Title: Mendelian randomization–inspired targeted polyphenol nanoplatform for synergistic osteoarthritis therapy
    Article Snippet: .. Sections were then incubated overnight at 4 °C with primary antibodies against ACAN, MMP13, TNF-α, IL-17, and SOX9 (1:200, Proteintech, Wuhan, China). ..

    Polymer:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Hematoxylin and eosin (H&E) (Servicebio, G1076), Safranin O–Fast Green (SO-FG) (Servicebio, G1053), and Toluidine Blue (TB) (Servicebio, G1032) staining evaluated cartilage morphology and matrix preservation, with histological grading performed using OARSI and Mankin scores. .. For immunohistochemistry, primary antibodies were COL2A1 (HUABIO, HA722733, 1:1000), SOX9 (Proteintech, 68350-1-Ig, 1:500), MMP3 (Servicebio, GB11132, 1:500), and ADAMTS1 (Affinity, DF13268, 1:100); sections were incubated with HRP-conjugated polymer secondary antibodies (Affinity, S0001), developed with DAB (Servicebio, G1212), and counterstained with hematoxylin. .. Whole-slide images were acquired on a digital scanner (Pannoramic MIDI, 3DHISTECH, Hungary), and quantification was performed in ImageJ.

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection-Driven cartilage regeneration.
    Article Snippet: Hematoxylin and eosin (H&E) (Servicebio, G1076), Safranin O–Fast Green (SO-FG) (Servicebio, G1053), and Toluidine Blue (TB) (Servicebio, G1032) staining evaluated cartilage morphology and matrix preservation, with histological grading performed using OARSI and Mankin scores. .. For immunohistochemistry, primary antibodies were COL2A1 (HUABIO, HA722733, 1:1000), SOX9 (Proteintech, 68350-1-Ig, 1:500), MMP3 (Servicebio, GB11132, 1:500), and ADAMTS1 (Affinity, DF13268, 1:100); sections were incubated with HRP-conjugated polymer secondary antibodies (Affinity, S0001), developed with DAB (Servicebio, G1212), and counterstained with hematoxylin. .. Whole-slide images were acquired on a digital scanner (Pannoramic MIDI, 3DHISTECH, Hungary), and quantification was performed in ImageJ.

    Immunofluorescence:

    Article Title: Injectable self-healing hydrogel for co-delivery of stem cells and growth factors in intervertebral disc regeneration
    Article Snippet: Intervertebral disc degeneration (IVDD) is a leading global cause of low back pain (LBP), for which current clinical interventions primarily provide symptomatic relief without restoring disc structure or function.. Although stem celland growth factor-based therapies have shown regenerative potential, their clinical efficacy is Jo ur na l P re -p ro of Journal Pre-proof 2 severely constrained by poor factor stability, rapid clearance, and low cell survival within the harsh nucleus pulposus (NP) microenvironment.. Here, we present an injectable, self-healing hydrogel platform for intervertebral disc regeneration that enables the co-delivery of transforming growth factor-β3 (TGF-β3) and adipose-derived mesenchymal stem cells (ADSCs).

    Concentration Assay:

    Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs
    Article Snippet: .. The information and the concentration of antibodies used in this study were listed as follows: Phospho-(Ser/Thr) PKA substrate antibody (1: 500, cat. no. 9621, CST, USA), GLP1R (1: 2,000, cat. no. 26196-1-AP, Proteintech, China), GIPR (1: 2,000, cat. no. 28322-1-AP, Proteintech), STRO-1 (1: 250, cat. no. 39-8401, Thermo Fisher Scientific), CD146 (1: 2,000, cat. no. 65181-1-Ig, Proteintech), Vimentin (1: 2,000, cat. no. 10366-1-AP, Proteintech), CREB1 (1: 2,000, cat. no. 67927-1-Ig, Proteintech), p-CREB1 (Ser133) (1: 2,000, cat. no. 28792-1-AP, Proteintech), ERK1/2 (1: 2,000, cat. no. 66192-1-Ig, Proteintech), p-ERK1/2 (Thr202/Tyr204) (1: 2,000, cat. no. 28733-1-AP, Proteintech), β-catenin (1: 2,000, cat. no. 51067-2-AP, Proteintech), p-β-catenin (Ser33) (1: 2,000, cat. no. 80067-1-RR, Proteintech), STAT3 (1: 2,000, cat. no. 10253-2-AP, Proteintech), p-STAT3 (Ser727) (1: 2,000, cat. no. 80199-2-RR, Proteintech), Ki-67 (1: 2,000, cat. no. 84192-4-RR, Proteintech), PCNA (1: 2,000, cat. no. 10205-2-AP, Proteintech), CCND3 (1: 2,000, cat. no. 26755-1-AP, Proteintech), β-actin (1: 2,000, cat. no. 66009-1- Ig, Proteintech), COL1A1 (1: 2,000, cat. no. 67288-1-Ig, Proteintech), OCN (1: 2,000, cat. no. 20277-1-AP, Proteintech), RUNX2 (1: 2,000, cat. no. 20700-1-AP, Proteintech), C/EBPα (1: 2,000, cat. no. 29388-1-AP, Proteintech), FABP4 (1: 2,000, cat. no. 12802-1-AP, Proteintech), PPARγ (1: 2,000, cat. no. 16643-1-AP, Proteintech), ACAN (1: 2,000, cat. no. 68350-1-Ig, Proteintech), COL2A1 (1: 2,000, cat. no. 28459-1-AP, Proteintech), SOX9 (1: 2,000, cat. no. 67439-1-Ig, Proteintech), MAP2 (1: 2,000, cat. no. 17490-1-AP, Proteintech), Nestin (1: 2,000, cat. no. 19483-1-AP, Proteintech), TUBB3 (1: 2,000, cat. no. 66375-1-Ig, Proteintech), IFIT1 (1: 2,000, cat. no. ab305301, Abcam), IFIT2 (1: 2,000, cat. no. 12604-1-AP, Proteintech), IFIT3 (1: 2,000, cat. no. 15201-1-AP, Proteintech), eIF3C (1: 2,000, cat. no. 12733-1-AP, Proteintech), eIF3E (1: 2,000, cat. no. 10899-1-AP, Proteintech), RPS3 (1: 2,000, cat. no. 66046-1-Ig, Proteintech), SPP1 (1: 2,000, cat. no. 22952-1-AP, Proteintech), FGF18 (1: 2,000, cat. no. 11495-1-AP, Proteintech), MMP14 (1: 2,000, cat. no. 14552-1-AP, Proteintech), BMP2 (1: 2,000, cat. no. 66383-1-Ig, Proteintech), horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1: 5,000, cat. no. SA00001-2, Proteintech), and HRP-conjugated goat anti-mouse IgG (1: 5,000, cat. no. SA00001-1, Proteintech). ..

    Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs.
    Article Snippet: .. The information and the concentration of antibodies used in this study were listed as follows: Phospho-(Ser/Thr) PKA substrate antibody (1: 500, cat. no. 9621, CST, USA), GLP1R (1: 2,000, cat. no. 26196-1-AP, Proteintech, China), GIPR (1: 2,000, cat. no. 28322-1-AP, Proteintech), STRO-1 (1: 250, cat. no. 39-8401, Thermo Fisher Scientific), CD146 (1: 2,000, cat. no. 65181-1-Ig, Proteintech), Vimentin (1: 2,000, cat. no. 10366-1-AP, Proteintech), CREB1 (1: 2,000, cat. no. 67927-1-Ig, Proteintech), p-CREB1 (Ser133) (1: 2,000, cat. no. 28792-1-AP, Proteintech), ERK1/2 (1: 2,000, cat. no. 66192-1-Ig, Proteintech), p-ERK1/2 (Thr202/Tyr204) (1: 2,000, cat. no. 28733-1-AP, Proteintech), β-catenin (1: 2,000, cat. no. 51067-2-AP, Proteintech), p-β-catenin (Ser33) (1: 2,000, cat. no. 80067-1-RR, Proteintech), STAT3 (1: 2,000, cat. no. 10253-2-AP, Proteintech), p-STAT3 (Ser727) (1: 2,000, cat. no. 80199-2-RR, Proteintech), Ki-67 (1: 2,000, cat. no. 84192-4-RR, Proteintech), PCNA (1: 2,000, cat. no. 10205-2-AP, Proteintech), CCND3 (1: 2,000, cat. no. 26755-1- AP, Proteintech), β-actin (1: 2,000, cat. no. 66009-1- Ig, Proteintech), COL1A1 (1: 2,000, cat. no. 67288-1-Ig, Proteintech), OCN (1: 2,000, cat. no. 20277-1-AP, Proteintech), RUNX2 (1: 2,000, cat. no. 20700-1-AP, Proteintech), C/EBPα (1: 2,000, cat. no. 29388- 1-AP, Proteintech), FABP4 (1: 2,000, cat. no. 12802-1-AP, Proteintech), PPARγ (1: 2,000, cat. no. 16643-1-AP, Proteintech), ACAN (1: 2,000, cat. no. 68350-1-Ig, Proteintech), COL2A1 (1: 2,000, cat. no. 28459-1-AP, Proteintech), SOX9 (1: 2,000, cat. no. 67439-1- Ig, Proteintech), MAP2 (1: 2,000, cat. no. 17490-1-AP, Proteintech), Nestin (1: 2,000, cat. no. 19483-1-AP, Proteintech), TUBB3 (1: 2,000, cat. no. 66375-1-Ig, Proteintech), IFIT1 (1: 2,000, cat. no. ab305301, Abcam), IFIT2 (1: 2,000, cat. no. 12604-1-AP, Proteintech), IFIT3 (1: 2,000, cat. no. 15201-1-AP, Proteintech), eIF3C (1: 2,000, cat. no. 12733-1-AP, Proteintech), eIF3E (1: 2,000, cat. no. 10899-1-AP, Proteintech), RPS3 (1: 2,000, cat. no. 66046-1-Ig, Proteintech), SPP1 (1: 2,000, cat. no. 22952-1-AP, Proteintech), FGF18 (1: 2,000, cat. no. 11495-1-AP, Proteintech), MMP14 (1: 2,000, cat. no. 14552-1-AP, Proteintech), BMP2 (1: 2,000, cat. no. 66383-1-Ig, Proteintech), horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1: 5,000, cat. no. SA00001-2, Proteintech), and HRP-conjugated goat anti-mouse IgG (1: 5,000, cat. no. SA00001-1, Proteintech). ..

    Blocking Assay:

    Article Title: Bioinspired scaffold recapitulating chondrogenic ontogeny and microenvironment for functional cartilage regeneration
    Article Snippet: .. After blocking the membranes for an hour at room temperature using fast blocking solution, the membranes were incubated with primary antibodies specific for SOX9 (1:2000, 67439-1-Ig, Proteintech), COL2 (1:200, NBP1-91056, Novus Biologicals), COL10 (1:500, 26984-1-AP, Proteintech), COL1 (1:2000, 67288-1-Ig, Proteintech), RUNX2 (1:200, 20700-1-AP, Proteintech), FGF2 (1:500, 11234-1-AP, Proteintech), FGF18 (1:500, 60341-1-Ig, Proteintech) and GAPDH (1:50000, 60004-1-Ig, Proteintech) for an entire night at 4 °C. ..



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    Molecular assessment of tendon repair and systemic biosafety. (A) Representative immunofluorescence images of inflammatory, matrix-degrading, tenogenic, and senescence markers, alongside macrophage phenotypes in repaired tendons. (B) Correlation analysis integrating molecular and functional recovery. Bar charts (left Y-axis) display the relative fluorescence intensity of the indicated markers, overlaid with line graphs (right Y-axis) showing the fold change in biomechanical properties (Ultimate Load and Tensile Modulus). Note the inverse correlation between SASP factors (IL-6, MMP13, P16) and mechanical strength. (C) Western blot analysis of the STING pathway, senescence indicators, and heterotopic ossification markers (OCN, <t>SOX9,</t> BMP-2). (D, E) Systemic biosafety evaluation via H&E staining of major organs (D) and blood biochemistry analysis (E) showing no toxicity. Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Sox9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat anti sox9  (R&D Systems)
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    R&D Systems goat anti sox9
    Molecular assessment of tendon repair and systemic biosafety. (A) Representative immunofluorescence images of inflammatory, matrix-degrading, tenogenic, and senescence markers, alongside macrophage phenotypes in repaired tendons. (B) Correlation analysis integrating molecular and functional recovery. Bar charts (left Y-axis) display the relative fluorescence intensity of the indicated markers, overlaid with line graphs (right Y-axis) showing the fold change in biomechanical properties (Ultimate Load and Tensile Modulus). Note the inverse correlation between SASP factors (IL-6, MMP13, P16) and mechanical strength. (C) Western blot analysis of the STING pathway, senescence indicators, and heterotopic ossification markers (OCN, <t>SOX9,</t> BMP-2). (D, E) Systemic biosafety evaluation via H&E staining of major organs (D) and blood biochemistry analysis (E) showing no toxicity. Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Goat Anti Sox9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of COL2A1, SOX9, MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of COL2A1, SOX9, MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Article Snippet: For immunohistochemistry, primary antibodies were COL2A1 (HUABIO, HA722733, 1:1000), SOX9 (Proteintech, 68350-1-Ig, 1:500), MMP3 (Servicebio, GB11132, 1:500), and ADAMTS1 (Affinity, DF13268, 1:100); sections were incubated with HRP-conjugated polymer secondary antibodies (Affinity, S0001), developed with DAB (Servicebio, G1212), and counterstained with hematoxylin.

    Techniques: Immunofluorescence, Expressing

    Expression analysis of cartilage-related proteins after treatment with AdHy@Pae. (A) Representative Western blot images showing ECM synthesis–related proteins (COL2A1, ACAN, COMP, and SOX9) and antioxidant markers (NRF2 and HO-1) in different groups. (B) Quantitative analysis of matrix-degrading enzymes (MMP13, ADAMTS5, ADAMTS1) and apoptosis-related proteins (BCL-2, Bax, and Caspase-3). Data are presented as mean ± SD (n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: Expression analysis of cartilage-related proteins after treatment with AdHy@Pae. (A) Representative Western blot images showing ECM synthesis–related proteins (COL2A1, ACAN, COMP, and SOX9) and antioxidant markers (NRF2 and HO-1) in different groups. (B) Quantitative analysis of matrix-degrading enzymes (MMP13, ADAMTS5, ADAMTS1) and apoptosis-related proteins (BCL-2, Bax, and Caspase-3). Data are presented as mean ± SD (n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Article Snippet: For immunohistochemistry, primary antibodies were COL2A1 (HUABIO, HA722733, 1:1000), SOX9 (Proteintech, 68350-1-Ig, 1:500), MMP3 (Servicebio, GB11132, 1:500), and ADAMTS1 (Affinity, DF13268, 1:100); sections were incubated with HRP-conjugated polymer secondary antibodies (Affinity, S0001), developed with DAB (Servicebio, G1212), and counterstained with hematoxylin.

    Techniques: Expressing, Western Blot

    In vivo validation of the chondroprotective and mitochondrial regulatory effects of AdHy@Pae after therapy for 4 weeks. (A) Representative immunohistochemical staining of cartilage sections for COL2A1, SOX9, MMP3, and ADAMTS1 in different groups (Sham, PBS, Hy, AdHy, and AdHy@Pae). (B) Quantitative analysis of the immunohistochemical staining intensity showing relative expression levels of anabolic (COL2A1, SOX9) and catabolic (MMP3, ADAMTS1) markers. (C) TEM images of chondrocytes showing mitochondrial ultrastructure in each group, with high-magnification views (bottom panels) indicating cristae integrity and membrane morphology. Data are presented as mean ± SD (n = 3); ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗P < 0.0001.

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: In vivo validation of the chondroprotective and mitochondrial regulatory effects of AdHy@Pae after therapy for 4 weeks. (A) Representative immunohistochemical staining of cartilage sections for COL2A1, SOX9, MMP3, and ADAMTS1 in different groups (Sham, PBS, Hy, AdHy, and AdHy@Pae). (B) Quantitative analysis of the immunohistochemical staining intensity showing relative expression levels of anabolic (COL2A1, SOX9) and catabolic (MMP3, ADAMTS1) markers. (C) TEM images of chondrocytes showing mitochondrial ultrastructure in each group, with high-magnification views (bottom panels) indicating cristae integrity and membrane morphology. Data are presented as mean ± SD (n = 3); ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗P < 0.0001.

    Article Snippet: For immunohistochemistry, primary antibodies were COL2A1 (HUABIO, HA722733, 1:1000), SOX9 (Proteintech, 68350-1-Ig, 1:500), MMP3 (Servicebio, GB11132, 1:500), and ADAMTS1 (Affinity, DF13268, 1:100); sections were incubated with HRP-conjugated polymer secondary antibodies (Affinity, S0001), developed with DAB (Servicebio, G1212), and counterstained with hematoxylin.

    Techniques: In Vivo, Biomarker Discovery, Immunohistochemical staining, Staining, Expressing, Membrane

    The effects of miR-101a on chondrogenic differentiation in ATDC5 cells. (A) Representative pictures of Alcian blue staining of ATDC5 cells after transfected with miR101a mimic and inhibitor. Magnification, 100×. (B, C) Representative pictures of Alizarin red staining and alkaline phosphatase (ALP) staining of ATDC5 cells after being transfected with miR-101a mimic and inhibitor. Magnification, 200×. (D, E) Reverse transcription quantitative real-time PCR analysis was utilized to evaluate the mRNA expression of Runx2 and Sox-9 in MCT and ATDC5 cells following miR-101a interference. (F) The protein levels of RUNX2 and SOX-9 were assessed by western blotting.

    Journal: Genes & Diseases

    Article Title: Role of miR-101a in targeting Cox-2 to attenuate chondrocyte hypertrophic differentiation and osteoarthritis progression

    doi: 10.1016/j.gendis.2025.101839

    Figure Lengend Snippet: The effects of miR-101a on chondrogenic differentiation in ATDC5 cells. (A) Representative pictures of Alcian blue staining of ATDC5 cells after transfected with miR101a mimic and inhibitor. Magnification, 100×. (B, C) Representative pictures of Alizarin red staining and alkaline phosphatase (ALP) staining of ATDC5 cells after being transfected with miR-101a mimic and inhibitor. Magnification, 200×. (D, E) Reverse transcription quantitative real-time PCR analysis was utilized to evaluate the mRNA expression of Runx2 and Sox-9 in MCT and ATDC5 cells following miR-101a interference. (F) The protein levels of RUNX2 and SOX-9 were assessed by western blotting.

    Article Snippet: The primary antibodies included COL10A1 (ab182563, Abcam, Massachusetts, USA), COX-2 (D223097, Biotechnology, Shanghai, China), MMP13 (ab51072, Abcam, Cambridge, Massachusetts, USA), RUNX2 (#12556, CST, USA), and SOX9 (#82630, CST, USA), which were prepared with primary antibody dilution (Yeasen Biotechnology, China). β-ACTIN was used as an internal control.

    Techniques: Staining, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot

    Molecular assessment of tendon repair and systemic biosafety. (A) Representative immunofluorescence images of inflammatory, matrix-degrading, tenogenic, and senescence markers, alongside macrophage phenotypes in repaired tendons. (B) Correlation analysis integrating molecular and functional recovery. Bar charts (left Y-axis) display the relative fluorescence intensity of the indicated markers, overlaid with line graphs (right Y-axis) showing the fold change in biomechanical properties (Ultimate Load and Tensile Modulus). Note the inverse correlation between SASP factors (IL-6, MMP13, P16) and mechanical strength. (C) Western blot analysis of the STING pathway, senescence indicators, and heterotopic ossification markers (OCN, SOX9, BMP-2). (D, E) Systemic biosafety evaluation via H&E staining of major organs (D) and blood biochemistry analysis (E) showing no toxicity. Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation

    doi: 10.1016/j.bioactmat.2026.01.004

    Figure Lengend Snippet: Molecular assessment of tendon repair and systemic biosafety. (A) Representative immunofluorescence images of inflammatory, matrix-degrading, tenogenic, and senescence markers, alongside macrophage phenotypes in repaired tendons. (B) Correlation analysis integrating molecular and functional recovery. Bar charts (left Y-axis) display the relative fluorescence intensity of the indicated markers, overlaid with line graphs (right Y-axis) showing the fold change in biomechanical properties (Ultimate Load and Tensile Modulus). Note the inverse correlation between SASP factors (IL-6, MMP13, P16) and mechanical strength. (C) Western blot analysis of the STING pathway, senescence indicators, and heterotopic ossification markers (OCN, SOX9, BMP-2). (D, E) Systemic biosafety evaluation via H&E staining of major organs (D) and blood biochemistry analysis (E) showing no toxicity. Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: After blocking for 1 h, membranes were incubated overnight at 4 °C with primary antibodies against STING (13647, CST, USA; A21051, Abclonal, China), p-STING (72971, CST, USA; AF7416, Affinity, China), IRF3 (ab68481, Abcam, UK), p-IRF3 (29047, CST, USA), P65 (A22331, Abclonal, China; 8242, CST, USA), p-P65 (AP0124, Abclonal, China), P53 (10442-1-AP, Proteintech, USA), SOX9 (sc-166505, Santa Cruz, USA), BMP-2 (ab284387, abcam, USA), OCN (sc-390877, Santa Cruz, USA), and iNOS (ab178945, Abcam, USA).

    Techniques: Immunofluorescence, Functional Assay, Fluorescence, Western Blot, Staining