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sollentuna  (MedChemExpress)


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    Structured Review

    MedChemExpress sollentuna
    Sollentuna, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sollentuna/JA2131/10__4103_slash_nrr__nrr___d___24___01102-69-33-32
    Average 94 stars, based on 2 article reviews
    sollentuna - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Functional Assay:

    Article Title: The protease ADAMTS5 controls ovarian cancer cell invasion, downstream of Rab25.
    Article Snippet: ON-TARGETplus SMARTpool siRNAs (Dharmacon, Horizon Discovery, Cambridge, UK): ADAMTS5 (L-005775-00-0005), Rab25 (L-010366-00-0005), siGENOME non-targeting control siRNA #4. .. The ADAMTS5 inhibitor (5-[[5-[[(4-Chlorophenyl)methyl]thio]-1methyl-3-(trifluoromethyl)-1H-pyrazol-4-yl]methylene]-2thioxo-4-thiazolidinone;(E)-5-((5-((4-Chlorobenzyl)thio)-1methyl-3-(trifluoromethyl)-1H-pyrazol-4 yl)methylene)-2thioxothiazolidin-4-one CAS no.: 929634-33-3) was from MedChemExpress, MedChemTronica, Sollentuna, Sweden reported to be a potent ADAMTS-5 inhibitor, with an IC50 of 1.1 lM and > 40-fold functional selectivity over ADAMTS4 [54,55]. ..

    other:


    Article Title: C9orf72 ALS-causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules.
    Article Snippet: To induce shRNA expression, cells were treated with 2 μg mL 1 doxycycline (HYN0565B; MedChemExpress, Sollentuna, Sweden) for 2 days, followed by 1 day in doxycycline-free medium prior to analysis.


    Construct:

    Article Title: Endothelin-1- and acetylcholine-mediated effects in human and rat vessels: impact of perivascular adipose tissue, diabetes, angiotensin II, and chemerin.
    Article Snippet: .. Next, CRCs were constructed to ET-1 (0.01 nmolL-0.1 μmol/L)., with or without preincubation with one or both of the following compounds for 30 min: 3 μmol/L α-NETA (2-[a-Naphthoyl] ethyltrimethylammonium iodide, MedChemExpress, Sollentuna, Sweden) or 20 μmol/L pravastatin (Sigma-Aldrich) or 10 μmol/L losartan (Sigma-Aldrich). ..

    Co-Immunoprecipitation Assay:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..

    Western Blot:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..

    Virus:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..

    shRNA:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..

    Recombinant:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..

    Reporter Assay:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..

    Countercurrent Chromatography:

    Article Title: IGF2BP1-HAX-1 positive feedback loop-mediated HAX-1 overexpression blocks autophagic flux and promotes chemoresistance in nasopharyngeal carcinoma.
    Article Snippet: .. Co-IP co-immunoprecipitation, WB western blot ◂ Reagent or Resource Source Identifier IGF2BP1 proteintech 22803-1-AP NEDD4 proteintech 21698-1-AP HA-Tag Cell Signaling Tech- nology #3724 Myc-Tag Cell Signaling Technology #2276 YTHDF1 proteintech 17479-1-AP YTHDF2 proteintech 24744-1-AP WTAP proteintech 60188-1-Ig GAPDH proteintech 10494-1-AP Bacterial and Virus Strains lentiviral HAX1- shRNA Shanghai Genechem Target seq: GAG TGA TGC AAG AAG TGA A lentiviral IGF2BP1shRNA Shanghai Genechem Target seq: ACA GTA GAG AAC TGT GAG CAA lentiviral Rab7AshRNA Shanghai Genechem Target seq: gaAAC AAG ATT GAC CTC GAA A RFP-GFP-tagged LC3 Shanghai Genechem tfLC3 Biological Samples Mouse tissues This paper N/A Human NPC speci- mens Affiliated Hospital of Nantong University N/A Chemicals, peptides, and recombinant proteins DMSO Fisher Scientific Cat#BP231-100 Cisplatin Jiangsu HANSOH PHARMA H20040813 Hochest Thermo Fisher Scientific 62249 Propidium Iodide Becton Dickinson and Company 556463 Fetal Bovine Serum Biological Industries 04-001-1ACS Reagent or Resource Source Identifier RPMI 1640 Biological Industries 01–100-1ACS TRIzol reagent BBI B511311 Uranyl acetate Polysciences, Hirschberg an der Bergstrasse, Germany 6159-44-0 Basic lead citrate Sigma-Aldrich, Taufkirchen, Germany 15326 Actinomycin D MCE, Sollentuna, Sweden HY-17559 Cycloheximide MCE, Sollentuna, Sweden HY-12320 MG-132 MEC, Sollentuna, Sweden HY-13259 AR6 buffer AKOYA, Marlborough, Massachusetts, USA AR600 CQ, chloroquine MCE HY-17589A Critical commercial assays EdU cell prolifera- tion assay Shanghai Beyotime C0078S CCK8 kit BBI E606335 LIVE/DEAD viabil- ity kit Shanghai Bestbio BB-4126 BCA protein assay kit Thermo Fisher Scientific 23227 Annexin V-FITC Apoptosis Detection Kit Beyotime C1063 Dual-Luciferase Reporter Assay Kit Beyotime RG088M Oligonucleotides Primer1 for HAX1, Forward CAG GAG GAG GGA TAC GTT TCC This paper N/A Primer1 for HAX1, Reverse CCC ATA TCG CTG AAG ATG CTATT This paper N/A Primer1 for preHAX1, Forward ATT ACA GTC GCC TGC CAA AC This paper N/A Primer1 for preHAX1, Reverse AGG TCA GGA GCT CAA AAG CA This paper N/A Primer1 for IGF2BP1, Forward GCG GCC AGT TCT TGG TCA A This paper N/A Primer1 for IGF2BP1, Reverse TTG GGC ACC GAA TGT TCA ATC This paper N/A Figure 5 Regulation of IGF2BP1 mRNA stability and translation by IGF2BP1. ..



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