anti smox  (Proteintech)

Journal: Journal of Inflammation Research

Article Title: Tellimagrandin II Stimulates Inflammasomes by Causing an Accumulation of 3-Aminopropanal, Which Promotes Apoptosis of Endometriotic Cells While Inhibiting Invasion

doi: 10.2147/JIR.S558146

Figure Lengend Snippet: Western blot analysis of EECs treated with Tellimagrandin II: ( a ) protein immunoblot; ( b ) ALDH7A1; ( c ) SMOX; ( d ) NLRP3; ( e ) Caspase-1; ( f ) β-catenin; ( g ) cleaved Caspase-3; ( h ) BAX; ( i ) MMP2; ( j ) TIMP1. Each experiment was repeated three times. *:P <0.05; **:P <0.01; ***:P <0.001; ****P <0.0001.

Article Snippet: Tellimagrandin II (Yongjian: B02179 ), Spermine Oxidase (SMOX) Polyclonal antibody (proteintech: 15052-1-AP), Aldehyde Dehydrogenase 7A1 (ALDH7A1) Polyclonal antibody (proteintech: 10368-1-AP), Anti-NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Rabbit pAb (Servicebio: GB114320-50), Recombinant Anti-beta Catenin (β-Catenin) antibody (Servicebio: GB150016-100), Anti-Matrix Metallopeptidase 2 (MMP2) Rabbit pAb (Servicebio: GB11130-100), Anti- Tissue Inhibitors of Metalloproteinase 1 (TIMP1) Rabbit pAb (proteintech: 16644-1-AP), Anti-Caspase-3 Rabbit pAb (Servicebio: GB115600-100), Anti-Bcl-2-associated X(Bax) Rabbit pAb (Servicebio: GB11690-100), Anti-Caspase-1 Rabbit pAb (Servicebio: GB11383-100), DMEM/F12 (Glutamine and HEPES)(M&C GENE: CM10092), Fetal Bovine Serum Gold (YEASEN: 40130ES76), penicillin streptomycin mixture (Servicebio: P1400), 1×PBS buffer (Solarbio: P1020), trypsin-EDTA digestion solution (Phenol Red)(Solarbio: T1320), CCK-8 kit (Sola rbio: CA1210), Annexin V-FITC Apoptosis detection kit (Beyotime: C1062S), 96-well cell culture plate (Servicebio:) CCP-96H, SWE matrix adhesive (Solarbio: G4131-5ML), crystal violet staining solution (Solarbio: G1014-50ML), paraformaldehyde fixative (Solarbio: G1101-500ML), Transwell chamber (Corning: WG3422) Cell culture flasks (Corning: 430639), 12Z EECs line (Bioharbor: LH-H337), DMSO (Servicebio: GC203006-10mL), Propidium Iodide (proteintech: CM18819), Flow cytometer (BD: FACSAria II), Gel imaging system (Alpha: 2200) Electrophoresis apparatus (Thermo: EC250-90), Thermo Corporation, USA; Pendulum type decolorizing shaker (model: DS-2S100), Nanodrop micro spectrophotometer (Illumina: Nanodrop2000), sequencing platform machine (Illumina: NovaSeq X Plus).

Techniques: Western Blot

Journal: Journal of Inflammation Research

Article Title: Tellimagrandin II Stimulates Inflammasomes by Causing an Accumulation of 3-Aminopropanal, Which Promotes Apoptosis of Endometriotic Cells While Inhibiting Invasion

doi: 10.2147/JIR.S558146

Figure Lengend Snippet: Molecular mechanism of Tellimagrandin II in the treatment of endometriosis. Tellimagrandin II (TII) may increase SMOX expression and decrease ALDH7 expression, leading to the accumulation of 3-aminopropanal in endometrial epithelial cells (EECs). The accumulated 3-aminopropanal can activate the inflammasome. Upon inflammasome activation, it may promote MMP2 expression via β-catenin and induce apoptosis by activating Caspase-1. However, whether TII inhibits invasion and promotes apoptosis in EECs through the inflammasome pathway requires further experimental verification.

Article Snippet: Tellimagrandin II (Yongjian: B02179 ), Spermine Oxidase (SMOX) Polyclonal antibody (proteintech: 15052-1-AP), Aldehyde Dehydrogenase 7A1 (ALDH7A1) Polyclonal antibody (proteintech: 10368-1-AP), Anti-NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Rabbit pAb (Servicebio: GB114320-50), Recombinant Anti-beta Catenin (β-Catenin) antibody (Servicebio: GB150016-100), Anti-Matrix Metallopeptidase 2 (MMP2) Rabbit pAb (Servicebio: GB11130-100), Anti- Tissue Inhibitors of Metalloproteinase 1 (TIMP1) Rabbit pAb (proteintech: 16644-1-AP), Anti-Caspase-3 Rabbit pAb (Servicebio: GB115600-100), Anti-Bcl-2-associated X(Bax) Rabbit pAb (Servicebio: GB11690-100), Anti-Caspase-1 Rabbit pAb (Servicebio: GB11383-100), DMEM/F12 (Glutamine and HEPES)(M&C GENE: CM10092), Fetal Bovine Serum Gold (YEASEN: 40130ES76), penicillin streptomycin mixture (Servicebio: P1400), 1×PBS buffer (Solarbio: P1020), trypsin-EDTA digestion solution (Phenol Red)(Solarbio: T1320), CCK-8 kit (Sola rbio: CA1210), Annexin V-FITC Apoptosis detection kit (Beyotime: C1062S), 96-well cell culture plate (Servicebio:) CCP-96H, SWE matrix adhesive (Solarbio: G4131-5ML), crystal violet staining solution (Solarbio: G1014-50ML), paraformaldehyde fixative (Solarbio: G1101-500ML), Transwell chamber (Corning: WG3422) Cell culture flasks (Corning: 430639), 12Z EECs line (Bioharbor: LH-H337), DMSO (Servicebio: GC203006-10mL), Propidium Iodide (proteintech: CM18819), Flow cytometer (BD: FACSAria II), Gel imaging system (Alpha: 2200) Electrophoresis apparatus (Thermo: EC250-90), Thermo Corporation, USA; Pendulum type decolorizing shaker (model: DS-2S100), Nanodrop micro spectrophotometer (Illumina: Nanodrop2000), sequencing platform machine (Illumina: NovaSeq X Plus).

Techniques: Expressing, Activation Assay

Journal: Journal of Inflammation Research

Article Title: Tellimagrandin II Stimulates Inflammasomes by Causing an Accumulation of 3-Aminopropanal, Which Promotes Apoptosis of Endometriotic Cells While Inhibiting Invasion

doi: 10.2147/JIR.S558146

Figure Lengend Snippet: Molecular docking diagram: ( a ) 3D representation of the docking between ALDH7A1 and Tellimagrandin II; ( b ) 2D representation of the docking between ALDH7A1 and Tellimagrandin II; ( c ) 3D representation of the docking between SMOX and Tellimagrandin II; ( d ) 2D representation of the docking between SMOX and Tellimagrandin II.

Article Snippet: Tellimagrandin II (Yongjian: B02179 ), Spermine Oxidase (SMOX) Polyclonal antibody (proteintech: 15052-1-AP), Aldehyde Dehydrogenase 7A1 (ALDH7A1) Polyclonal antibody (proteintech: 10368-1-AP), Anti-NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Rabbit pAb (Servicebio: GB114320-50), Recombinant Anti-beta Catenin (β-Catenin) antibody (Servicebio: GB150016-100), Anti-Matrix Metallopeptidase 2 (MMP2) Rabbit pAb (Servicebio: GB11130-100), Anti- Tissue Inhibitors of Metalloproteinase 1 (TIMP1) Rabbit pAb (proteintech: 16644-1-AP), Anti-Caspase-3 Rabbit pAb (Servicebio: GB115600-100), Anti-Bcl-2-associated X(Bax) Rabbit pAb (Servicebio: GB11690-100), Anti-Caspase-1 Rabbit pAb (Servicebio: GB11383-100), DMEM/F12 (Glutamine and HEPES)(M&C GENE: CM10092), Fetal Bovine Serum Gold (YEASEN: 40130ES76), penicillin streptomycin mixture (Servicebio: P1400), 1×PBS buffer (Solarbio: P1020), trypsin-EDTA digestion solution (Phenol Red)(Solarbio: T1320), CCK-8 kit (Sola rbio: CA1210), Annexin V-FITC Apoptosis detection kit (Beyotime: C1062S), 96-well cell culture plate (Servicebio:) CCP-96H, SWE matrix adhesive (Solarbio: G4131-5ML), crystal violet staining solution (Solarbio: G1014-50ML), paraformaldehyde fixative (Solarbio: G1101-500ML), Transwell chamber (Corning: WG3422) Cell culture flasks (Corning: 430639), 12Z EECs line (Bioharbor: LH-H337), DMSO (Servicebio: GC203006-10mL), Propidium Iodide (proteintech: CM18819), Flow cytometer (BD: FACSAria II), Gel imaging system (Alpha: 2200) Electrophoresis apparatus (Thermo: EC250-90), Thermo Corporation, USA; Pendulum type decolorizing shaker (model: DS-2S100), Nanodrop micro spectrophotometer (Illumina: Nanodrop2000), sequencing platform machine (Illumina: NovaSeq X Plus).

Techniques:

Journal: Scientific Reports

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein

doi: 10.1038/s41598-025-96388-7

Figure Lengend Snippet: NRF1 directly binds to Smox genomic locus to suppress the expression. ( A ) Putative NRF1-binding sites around the Smox genomic region are predicted from the ChIP-seq data for MAFK, NRF1, and NRF2. ChIP-seq profiles of MAFK from Raw 264.7 cells and ES cells, NRF1 from MEF cells, NRF2 from Macrophages obtained from the Peak Browser of ChIP-Atlas, http://chip-atlas.org . Significant peaks that contain ARE consensus sequence are depicted as horizontal black bars and designed site 1, site 2, site 3, and site 4. Smox gene is constructed by seven parts of exon indicated with black and intron indicated with gray in Fig. A. Start codon in present in Exon 3. ( B ) The ARE sequences from predicted NRF1-binding sites indicated in panel A are aligned. Nucleotides that are conserved or similar between site 1, site 2, site 3, and site 4 are indicated as white letters on a black background or black letters on a gray background, respectively. ( C ) ChIP-qPCR experiment performed with an anti-NRF1 antibody. Specific primer sets were employed in qPCR from predicted DNA to detect site 1, site 2, site 3, site 4. Txs , genomic region in the third intron of Txs was used as a negative control. Analysis of each of the four groups of mice (n = 6). One of the triplicates of experiments is displayed, and results are expressed as means ± SEM. The statistical significance of results, compared with values of Cont, was calculated using one-way ANOVA with Dunnett’s test. * , significant change, P = 0.05 to 0.01; ** , P = 0.01 to 0.001. ( D ) Schematic illustration of reporter constructs used in panels E. ( E ) Luciferase reporter gene assay to measure Smox transactivation in Hepa1c1c7 transfected shLacZ (open bars) or Hepa1c1c7 transfected shNrf1 (filled bars) cells. These cells were transfected with the SMOX reporter constructs depicted in panel E, and firefly luciferase activity was determined the after 48 h recovery. Data were standardized with Renilla luciferase activity.

Article Snippet: Small interfering RNA (siRNA), which targets the mouse Smox was obtained from Thermo Fisher Scientific, and its sequence is shown in Supplementary Table .

Techniques: Expressing, Binding Assay, ChIP-sequencing, Sequencing, Construct, ChIP-qPCR, Negative Control, Luciferase, Reporter Gene Assay, Transfection, Activity Assay

Journal: Scientific Reports

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein

doi: 10.1038/s41598-025-96388-7

Figure Lengend Snippet: Decreasing the acrolein conjugated protein content in Smox knockdown MEF cells. ( A ) Immunoblot analysis of antibody for SMOX and Acrolein conjugated Lysine. Aliquot of total lysates from cells per equal amount of siRNA transected group were separated by SDS-PAGE (8% gel). Equal loading was assessed by probing the blots with antibody against β-ACTIN which used as control in the WT- and N1KO-MEF. Protein molecular weights are shewed right side of the blots. ( B ) The relative band intensity of acrolein conjugated protein and SMOX were determined by ImageJ Fiji, each band intensity were normalized with that of β-actin. One of the triplicates (n = 3) of experiments is displayed and results are expressed as standard errors of the means ± SEM. The statistical significance of results, compared with values from the control was calculated using one-way ANOVA.

Article Snippet: Small interfering RNA (siRNA), which targets the mouse Smox was obtained from Thermo Fisher Scientific, and its sequence is shown in Supplementary Table .

Techniques: Knockdown, Western Blot, SDS Page, Control

Journal: Scientific Reports

Article Title: NF-E2-related factor 1 suppresses the expression of a spermine oxidase and the production of highly reactive acrolein

doi: 10.1038/s41598-025-96388-7

Figure Lengend Snippet: Decreasing the free acrolein content in Smox knockdown WT- and N1KO-MEF cells. ( A ) Fluorescence image of siRNA targeting Smox or control siRNA transfectant WT- and N1KO-MEF were performed with AcroleinRED and Hoechst. Images were obtained by inverted fluorescent microscope and processed through THUNDER Imaging Systems after 72 h of transfection. Bright field images were obtained phase contrast image, Red free acrolein, Blue nucleus. Magnification: × 200. Scale bar = 132 µm. B , Fluorescence intensities were quantified using ImageJ fiji. ( B ) The content of acrolein in each individual cell were calculated using 1000 cells in image fields. Each dot represented as fluorescent intensity/cell. The values are indicated as means ± SEM. The statistical significance of results, compared with values from the control was calculated using one-way ANOVA with Dunnett’s test. $$$$ , P = 0.005 to 0.0001.

Article Snippet: Small interfering RNA (siRNA), which targets the mouse Smox was obtained from Thermo Fisher Scientific, and its sequence is shown in Supplementary Table .

Techniques: Knockdown, Fluorescence, Control, Transfection, Microscopy, Imaging