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Bethyl smg7
Smg7, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smg7/SMG7+Antibody/10__1038_slash_s41556___026___01912___0-248-19-21
Average 93 stars, based on 27 article reviews
smg7 - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Western Blot:

Article Title: A transcriptional roadmap for 2C-like-to-pluripotent state transition.
Article Snippet: Cellular protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, catalog no. 78501) with protease inhibitor (Roche, catalog no. 4693159001). .. Western blotting was performed with 4 to 12% bis-tris gradient gel (Invitrogen, NP0322BOX) with the following antibodies: Smg7 (1:1000, Bethyl Laboratories, A302-170), Upf1 (1:1000, Bethyl Laboratories, A300036), Gapdh (1:10,000, Ambion, AM4300, 6C5), goat anti-mouse immunoglobulin G (IgG) (H + L) secondary antibody, horseradish peroxidase (HRP) (1:10,000, Invitrogen, 31430), and goat anti-rabbit IgG (H + L) secondary antibody, HRP (1:10,000, Invitrogen, 31460). ..

Article Title: UPF1 promotes rapid degradation of m 6 A-containing RNAs.
Article Snippet: .. The following primary antibodies were used for IP and western blotting [listed in the format ‘‘target protein name (catalog number, supplier)’’]: YTHDF1 (17479-1-AP, Proteintech), YTHDF2 (24744-1-AP, Proteintech), YTHDF3 (sc-377119, Santa Cruz Biotech), HRSP12 (PA5-31352, Thermo Scientific), POP1 (12029-1-AP, Proteintech), CNOT1 (14276-1-AP, Proteintech), SMG1 (A300394A, Bethyl Laboratories), SMG5 (ab33033, Abcam), SMG7 (A302-170A, Bethyl Laboratories), DCP1A (D5694, Sigma), FLAG (A8592, Sigma), GST (A190-122A, Bethyl Laboratories), HA (11867431001, Roche), b-actin (A5441, Sigma), and MYC (OP10L, Calbiochem). ..

Article Title: Nonsense-mediated mRNA decay factor UPF1 promotes aggresome formation
Article Snippet: .. Antibodies against the following proteins were purchased [listed in the format “protein name (catalog number, supplier, dilution fold)”]: FLAG (DYKDDDDK; 14793, Cell Signaling Technology, 1:50, or A8592, Sigma, 1:5000), HA (3724, Cell Signaling Technology, 1:200), Myc (9E10; OP10L, Calbiochem, 1:5000, or 2272, Cell Signaling Technology, 1:200), GFP (sc-9996, Santa Cruz Biotechnology, 1:50), DCTN1 (p150 glued ; 610474, BD Biosciences, 1:250), eEF1A1 (CBP-KK1; EF1α; 05-235, Merck Millipore, 1:1000), DCP1A (D5444, Sigma, 1:1000), Y14 (RBM8A; MAB2484, Abnova, 1:1000), SMG1 (A300-394A, Bethyl Laboratories, 1:1000), SMG5 (ab33033, Abcam, 1:500), SMG7 (A302-170A, Bethyl Laboratories, 1:2000), ATM (A300-299A, Bethyl Laboratories, 1:1000), DNA-PKcs (A300-517A, Bethyl Laboratories, 1:1000), p-(S/T)Q ATM/ATR substrate (2851, Cell Signaling Technology, 1:1000), puromycin (12D10; MABE343, Merck Millipore, 1:10000 for western blotting or 1:400 for immunostaining), eIF3b (sc-16377, Santa Cruz Biotechnology, 1:1000), γ-tubulin (sc-17788, Santa Cruz Biotechnology, 1:20), α-tubulin (sc-53030, Santa Cruz Biotechnology, 1:100), GST (A190-122A, Bethyl Laboratories, 1:8000), His (27-4710-01, GE Healthcare, 1:3000), β-actin (A5441, Sigma, 1:10,000), and GAPDH (LF-PA0212, AbFrontier, 1:10,000). ..

Article Title: A transcriptional roadmap for 2C-like–to–pluripotent state transition
Article Snippet: Cellular protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, catalog no. 78501) with protease inhibitor (Roche, catalog no. 4693159001). .. Western blotting was performed with 4 to 12% bis-tris gradient gel (Invitrogen, NP0322BOX) with the following antibodies: Smg7 (1:1000, Bethyl Laboratories, A302-170), Upf1 (1:1000, Bethyl Laboratories, A300-036), Gapdh (1:10,000, Ambion, AM4300, 6C5), goat anti-mouse immunoglobulin G (IgG) (H + L) secondary antibody, horseradish peroxidase (HRP) (1:10,000, Invitrogen, 31430), and goat anti-rabbit IgG (H + L) secondary antibody, HRP (1:10,000, Invitrogen, 31460). ..

other:

Article Title: SMG6 localizes to the chromatoid body and shapes the male germ cell transcriptome to drive spermatogenesis
Article Snippet: Primary antibodies used in this study were: DDX25 (sc-51271) and Hiwi (sc-22685) from Santa Cruz Biotechnology; DDX4 (ab13840), EIF4A3 (ab32485), and SMG6 (ab87539) from Abcam; PIWIL1 (G82) from Cell Signaling Technology; FYCO1 (HPA035526 and SAB1400697) from Sigma-Aldrich; GAPDH (5G4) from HyTest; γH2AX antibody (05-636) and PIWIL2 clone 13E-3 (MABE363) from Millipore; SMG7 (A302-170A) from Bethyl Laboratories and hVasa (AF2030-SP) from R&D systems.

Immunostaining:

Article Title: Nonsense-mediated mRNA decay factor UPF1 promotes aggresome formation
Article Snippet: .. Antibodies against the following proteins were purchased [listed in the format “protein name (catalog number, supplier, dilution fold)”]: FLAG (DYKDDDDK; 14793, Cell Signaling Technology, 1:50, or A8592, Sigma, 1:5000), HA (3724, Cell Signaling Technology, 1:200), Myc (9E10; OP10L, Calbiochem, 1:5000, or 2272, Cell Signaling Technology, 1:200), GFP (sc-9996, Santa Cruz Biotechnology, 1:50), DCTN1 (p150 glued ; 610474, BD Biosciences, 1:250), eEF1A1 (CBP-KK1; EF1α; 05-235, Merck Millipore, 1:1000), DCP1A (D5444, Sigma, 1:1000), Y14 (RBM8A; MAB2484, Abnova, 1:1000), SMG1 (A300-394A, Bethyl Laboratories, 1:1000), SMG5 (ab33033, Abcam, 1:500), SMG7 (A302-170A, Bethyl Laboratories, 1:2000), ATM (A300-299A, Bethyl Laboratories, 1:1000), DNA-PKcs (A300-517A, Bethyl Laboratories, 1:1000), p-(S/T)Q ATM/ATR substrate (2851, Cell Signaling Technology, 1:1000), puromycin (12D10; MABE343, Merck Millipore, 1:10000 for western blotting or 1:400 for immunostaining), eIF3b (sc-16377, Santa Cruz Biotechnology, 1:1000), γ-tubulin (sc-17788, Santa Cruz Biotechnology, 1:20), α-tubulin (sc-53030, Santa Cruz Biotechnology, 1:100), GST (A190-122A, Bethyl Laboratories, 1:8000), His (27-4710-01, GE Healthcare, 1:3000), β-actin (A5441, Sigma, 1:10,000), and GAPDH (LF-PA0212, AbFrontier, 1:10,000). ..



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Rex interacts with Upf1 and SMG5/7 and contributes to the substitution of Upf3B and Upf3A. ( A ). NMD regulatory complex proteins interacting with Rex in HEK293FT cells were investigated by GST-Rex coimmunoprecipitation assay. The results showed that Rex interacts not only with Upf1 but also with SMG5, <t>SMG7</t> and Upf3B. In contrast, Rex did not interact with Upf3A, the isoform of Upf3B. ( B ). Amount of Upf3B which binds to Upf2 in the NMD complex in the presence or absence of Rex detected by coimmunoprecipitation assay of GST-Upf2 with His-Upf3B and HA-Upf3A in HEK293FT cells. The amount of Upf3B interacting with Upf2 was reduced in the presence of Rex, and instead the interaction with Upf3A, the less active form of Upf3, was increased.
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Image Search Results


Rex interacts with Upf1 and SMG5/7 and contributes to the substitution of Upf3B and Upf3A. ( A ). NMD regulatory complex proteins interacting with Rex in HEK293FT cells were investigated by GST-Rex coimmunoprecipitation assay. The results showed that Rex interacts not only with Upf1 but also with SMG5, SMG7 and Upf3B. In contrast, Rex did not interact with Upf3A, the isoform of Upf3B. ( B ). Amount of Upf3B which binds to Upf2 in the NMD complex in the presence or absence of Rex detected by coimmunoprecipitation assay of GST-Upf2 with His-Upf3B and HA-Upf3A in HEK293FT cells. The amount of Upf3B interacting with Upf2 was reduced in the presence of Rex, and instead the interaction with Upf3A, the less active form of Upf3, was increased.

Journal: Viruses

Article Title: Elucidation of the Mechanism of Host NMD Suppression by HTLV-1 Rex: Dissection of Rex to Identify the NMD Inhibitory Domain

doi: 10.3390/v14020344

Figure Lengend Snippet: Rex interacts with Upf1 and SMG5/7 and contributes to the substitution of Upf3B and Upf3A. ( A ). NMD regulatory complex proteins interacting with Rex in HEK293FT cells were investigated by GST-Rex coimmunoprecipitation assay. The results showed that Rex interacts not only with Upf1 but also with SMG5, SMG7 and Upf3B. In contrast, Rex did not interact with Upf3A, the isoform of Upf3B. ( B ). Amount of Upf3B which binds to Upf2 in the NMD complex in the presence or absence of Rex detected by coimmunoprecipitation assay of GST-Upf2 with His-Upf3B and HA-Upf3A in HEK293FT cells. The amount of Upf3B interacting with Upf2 was reduced in the presence of Rex, and instead the interaction with Upf3A, the less active form of Upf3, was increased.

Article Snippet: Cambridge, UK), Upf3A (#H00065110-M06, Abnova, Corp.), Upf3B (#A303-688A, Bethyl Laboratories, Inc., Montgomery, TX, USA), SMG1 (#9149S, Cell Signaling Technology, Inc.), SMG5 (#ab-129107, Abcam, Plc.), SMG6 (#sc-50984-R, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), SMG7 (#sc-134857, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), β-Actin (#sc-69879, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FLAG (#F1804, SIGMA-Aldrich, Merck KgaA, Darmstadt, Germany), GST (#Cytiva 27-4577-01, SIGMA-Aldrich, Merck KgaA, Darmstadt, Germany), and monoclonal antibodies against HTLV-1 proteins (Tax, Rex, Gag(p53), and Env(gp24)) were generated by Yuetsu Tanaka at the University of Ryukyus.

Techniques: Co-Immunoprecipitation Assay