smg7 (Bethyl)
Structured Review
Smg7, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smg7/SMG7+Antibody/10__1038_slash_s41556___026___01912___0-248-19-21
Average 93 stars, based on 27 article reviews
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Western Blot:Article Title: A transcriptional roadmap for 2C-like-to-pluripotent state transition. Article Snippet: Cellular protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, catalog no. 78501) with protease inhibitor (Roche, catalog no. 4693159001). .. Western blotting was performed with 4 to 12% bis-tris gradient gel (Invitrogen, NP0322BOX) with the following antibodies: Article Title: UPF1 promotes rapid degradation of m 6 A-containing RNAs. Article Snippet: .. The following primary antibodies were used for IP and western blotting [listed in the format ‘‘target protein name (catalog number, supplier)’’]: YTHDF1 (17479-1-AP, Proteintech), YTHDF2 (24744-1-AP, Proteintech), YTHDF3 (sc-377119, Santa Cruz Biotech), HRSP12 (PA5-31352, Thermo Scientific), POP1 (12029-1-AP, Proteintech), CNOT1 (14276-1-AP, Proteintech), SMG1 (A300394A, Bethyl Laboratories), SMG5 (ab33033, Abcam), Article Title: Nonsense-mediated mRNA decay factor UPF1 promotes aggresome formation Article Snippet: .. Antibodies against the following proteins were purchased [listed in the format “protein name (catalog number, supplier, dilution fold)”]: FLAG (DYKDDDDK; 14793, Cell Signaling Technology, 1:50, or A8592, Sigma, 1:5000), HA (3724, Cell Signaling Technology, 1:200), Myc (9E10; OP10L, Calbiochem, 1:5000, or 2272, Cell Signaling Technology, 1:200), GFP (sc-9996, Santa Cruz Biotechnology, 1:50), DCTN1 (p150 glued ; 610474, BD Biosciences, 1:250), eEF1A1 (CBP-KK1; EF1α; 05-235, Merck Millipore, 1:1000), DCP1A (D5444, Sigma, 1:1000), Y14 (RBM8A; MAB2484, Abnova, 1:1000), SMG1 (A300-394A, Bethyl Laboratories, 1:1000), SMG5 (ab33033, Abcam, 1:500), Article Title: A transcriptional roadmap for 2C-like–to–pluripotent state transition Article Snippet: Cellular protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, catalog no. 78501) with protease inhibitor (Roche, catalog no. 4693159001). .. Western blotting was performed with 4 to 12% bis-tris gradient gel (Invitrogen, NP0322BOX) with the following antibodies: other:Article Title: SMG6 localizes to the chromatoid body and shapes the male germ cell transcriptome to drive spermatogenesis Article Snippet: Primary antibodies used in this study were: DDX25 (sc-51271) and Hiwi (sc-22685) from Santa Cruz Biotechnology; DDX4 (ab13840), EIF4A3 (ab32485), and SMG6 (ab87539) from Abcam; PIWIL1 (G82) from Cell Signaling Technology; FYCO1 (HPA035526 and SAB1400697) from Sigma-Aldrich; GAPDH (5G4) from HyTest; γH2AX antibody (05-636) and PIWIL2 clone 13E-3 (MABE363) from Millipore; SMG7 (A302-170A) from Bethyl Laboratories and hVasa (AF2030-SP) from R&D systems. Immunostaining:Article Title: Nonsense-mediated mRNA decay factor UPF1 promotes aggresome formation Article Snippet: .. Antibodies against the following proteins were purchased [listed in the format “protein name (catalog number, supplier, dilution fold)”]: FLAG (DYKDDDDK; 14793, Cell Signaling Technology, 1:50, or A8592, Sigma, 1:5000), HA (3724, Cell Signaling Technology, 1:200), Myc (9E10; OP10L, Calbiochem, 1:5000, or 2272, Cell Signaling Technology, 1:200), GFP (sc-9996, Santa Cruz Biotechnology, 1:50), DCTN1 (p150 glued ; 610474, BD Biosciences, 1:250), eEF1A1 (CBP-KK1; EF1α; 05-235, Merck Millipore, 1:1000), DCP1A (D5444, Sigma, 1:1000), Y14 (RBM8A; MAB2484, Abnova, 1:1000), SMG1 (A300-394A, Bethyl Laboratories, 1:1000), SMG5 (ab33033, Abcam, 1:500), |
