slc5a7  (Proteintech)

Journal: Journal of Neuroinflammation

Article Title: Selective vulnerability of the aging cholinergic system to amyloid pathology revealed by induced APP overexpression

doi: 10.1186/s12974-025-03682-2

Figure Lengend Snippet: Selective vulnerability of the aged mouse cholinergic system to induced mutant APP expression. a Whole-brain RNA sequencing identified genes with a main effect of mutant APP expression, a main effect of age, and/or a significant age × APP interaction (FDR < 0.05). Samples used for differential gene expression analyses included 2 male and 2 female 18mo controls, 2 male and 2 female APP 6 → 18mo, 2 male and 1 female 24mo controls, and 2 male and 2 female APP 12 → 24mo. Volcano plots showing differentially expressed genes driven by ( b ) mutant APP expression, ( c ) age, and ( d ) an age × APP interaction in the 24-month-old mice (APP 12 → 24mo vs. 24-month-old control). e Correlation analysis of APP effect sizes on gene expression between 24-month-old and 18-month-old animals. f Overview of age and APP-related changes in brain cell lineage marker genes. mRNA levels of ( g ) Chat , ( h ) Slc5a7 , and ( i ) Slc18a3 were measured by qPCR in 18mo control (4 males and 4 females), APP 6 → 18mo (4 males and 4 females), 24mo control (4 males and 3–4 females), and APP 12 → 24mo (4 males and 3–4 females). j Representative western blot images of cholinergic marker proteins. Protein levels of ( k ) ChAT and ( l ) SLC5A7 were specifically reduced in APP-induced 24-month-old mice. Sample size corresponds to 5 male and 5 female 18mo controls, 5 male and 5–6 female APP 6 → 18mo, 4–5 male and 5 female 24mo controls, and 4–5 male and 6 female APP 12 → 24mo. Individual data points and group means ± S.E.M. are presented. ** p < 0.01, **** p < 0.0001

Article Snippet: Gene expression of Chat (Mm01221882_m1), Slc5a7 (Mm00452075_m1), and Slc18a3 (Mm00491465_s1) was quantified using TaqMan Gene Expression Assays (Applied Biosystems, Darmstadt, Germany) on a StepOnePlus Real-Time PCR System (Applied Biosystems, Darmstadt, Germany).

Techniques: Mutagenesis, Expressing, RNA Sequencing, Gene Expression, Control, Marker, Western Blot

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: CircFBXW4 Suppresses Colorectal Cancer Progression by Regulating the MiR-338-5p/SLC5A7 Axis.

doi: 10.1002/advs.202300129

Figure Lengend Snippet: Figure 7. CircFBXW4 regulates SLC5A7 expression by acting as a sponge for miR-338-5p. A) Relative expression of candidate mRNAs in SW480 cells transfected with the miR-338-5p mimic. B) Relative expression of candidate mRNAs in SW620 cells transfected with the miR-338-5p inhibitor. C) Relative protein levels of SLC5A7 in CRC cells transfected with the miR-338-5p mimic or inhibitor. D) The relative expression of SLC5A7 in CRC tissues and matched adjacent normal tissues was determined by qRT‒PCR (n = 40). E) Pearson correlation analysis between the expression levels of miR-338-5p and SLC5A7 in our own patient cohort (n = 40). F) Schematic illustration of the SLC5A7-WT and SLC5A7-MUT luciferase reporter vectors. G) Relative luciferase activity was measured in 293T cells after cotransfection with SLC5A7-WT or SLC5A7-MUT and the miR-338-5p mimic or NC. H) The relative mRNA and protein expression levels of SLC5A7 were measured by qRT‒PCR and western blotting in SW480 cells transfected with si-NC or si-circFBXW4 with or without the miR-338-5p inhibitor. I) The relative mRNA and protein levels of SLC5A7 were measured by qRT‒PCR and western blotting in SW620 cells transfected with vector or the circFBXW4-OE plasmid with or without the miR-338-5p mimic. The data are shown as the means ± SDs; *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The primary antibodies used to detect Ki-67, PCNA, and Caspase3 were purchased from ABclonal (China), and the primary antibody used to detect SLC5A7 was purchased from ProteinTech (China).

Techniques: Expressing, Transfection, Luciferase, Activity Assay, Cotransfection, Western Blot, Plasmid Preparation

Journal: Advanced Science

Article Title: CircFBXW4 Suppresses Colorectal Cancer Progression by Regulating the MiR‐338‐5p/SLC5A7 Axis

doi: 10.1002/advs.202300129

Figure Lengend Snippet: Characterization and clinical significance of circFBXW4. A) Schematic illustration of the genomic location of circFBXW4 and its generation by backsplicing. B) CircFBXW4 was detected in SW480 and SW620 cells by qRT‒PCR with divergent and convergent primers and validated by agarose gel electrophoresis. The head‐to‐tail splicing of circFBXW4 was confirmed by Sanger sequencing. C) The expression of circFBXW4 and linear FBXW4 mRNA in SW480 and SW620 cells treated with RNase R for different durations was analyzed by qRT‒PCR. D) The expression of circFBXW4 and linear FBXW4 mRNA in SW480 and SW620 cells was measured by qRT‒PCR after treatment with actinomycin D for 12 h and 24 h. E) The expression of circFBXW4 in the cytoplasm and nucleus was measured by qRT‒PCR. F) The FISH assay results showed that circFBXW4 was localized predominantly in the cytoplasm in CRC cell lines. Nuclei were stained blue with DAPI, and cytoplasmic circFBXW4 was stained red. G) ROC curves of circFBXW4 and SLC5A7 for distinguishing between CRC tissues and non‐CRC tissues. H) ROC curves of CEA and CA19‐9 for distinguishing between CRC patients and healthy donors. * P < 0.05, *** P < 0.001.

Article Snippet: After blocking, the membrane was incubated first with a primary antibody targeting SLC5A7 (1: 1000, ABclonal, China, A8247) and subsequently with a secondary antibody.

Techniques: Agarose Gel Electrophoresis, Sequencing, Expressing, Staining

Journal: Advanced Science

Article Title: CircFBXW4 Suppresses Colorectal Cancer Progression by Regulating the MiR‐338‐5p/SLC5A7 Axis

doi: 10.1002/advs.202300129

Figure Lengend Snippet: CircFBXW4 regulates SLC5A7 expression by acting as a sponge for miR‐338‐5p. A) Relative expression of candidate mRNAs in SW480 cells transfected with the miR‐338‐5p mimic. B) Relative expression of candidate mRNAs in SW620 cells transfected with the miR‐338‐5p inhibitor. C) Relative protein levels of SLC5A7 in CRC cells transfected with the miR‐338‐5p mimic or inhibitor. D) The relative expression of SLC5A7 in CRC tissues and matched adjacent normal tissues was determined by qRT‒PCR (n = 40). E) Pearson correlation analysis between the expression levels of miR‐338‐5p and SLC5A7 in our own patient cohort (n = 40). F) Schematic illustration of the SLC5A7‐WT and SLC5A7‐MUT luciferase reporter vectors. G) Relative luciferase activity was measured in 293T cells after cotransfection with SLC5A7‐WT or SLC5A7‐MUT and the miR‐338‐5p mimic or NC. H) The relative mRNA and protein expression levels of SLC5A7 were measured by qRT‒PCR and western blotting in SW480 cells transfected with si‐NC or si‐circFBXW4 with or without the miR‐338‐5p inhibitor. I) The relative mRNA and protein levels of SLC5A7 were measured by qRT‒PCR and western blotting in SW620 cells transfected with vector or the circFBXW4‐OE plasmid with or without the miR‐338‐5p mimic. The data are shown as the means ± SDs; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: After blocking, the membrane was incubated first with a primary antibody targeting SLC5A7 (1: 1000, ABclonal, China, A8247) and subsequently with a secondary antibody.

Techniques: Expressing, Transfection, Luciferase, Activity Assay, Cotransfection, Western Blot, Plasmid Preparation

Journal: Advanced Science

Article Title: CircFBXW4 Suppresses Colorectal Cancer Progression by Regulating the MiR‐338‐5p/SLC5A7 Axis

doi: 10.1002/advs.202300129

Figure Lengend Snippet: CircFBXW4 suppresses CRC tumor growth in vivo. A) The expression of circFBXW4 was measured by qRT‒PCR in SW620 cells stably overexpressing circFBXW4. B) Comparison of tumor volume curves in the circFBXW4‐OE, vector, circFBXW4‐OE + miR‐338‐5p mimic, circFBXW4‐OE + miR‐338‐5p control groups. C) Comparison of tumor weights in the circFBXW4‐OE, vector, circFBXW4‐OE + miR‐338‐5p mimic, circFBXW4‐OE + miR‐338‐5p control groups. D) Representative images of CRC xenograft tumors in each group. E) Bioluminescent Imaging (BLI) of mice in the circFBXW4‐OE, vector, circFBXW4‐OE + miR‐338‐5p mimic, circFBXW4‐OE + miR‐338‐5p control groups. F) Changes in Ki67, PCNA, Caspase3, and SLC5A7 expression in xenograft tumors were detected by IHC staining. G) Schematic diagram describing the proposed regulatory mechanism underlying the circFBXW4‐dependent regulatory network and the miR‐338‐5p/SLC5A7 axis in colorectal carcinogenesis. CircFBXW4 functions as a sponge for miR‐338‐5p and in turn promotes the expression of SLC5A7. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: After blocking, the membrane was incubated first with a primary antibody targeting SLC5A7 (1: 1000, ABclonal, China, A8247) and subsequently with a secondary antibody.

Techniques: In Vivo, Expressing, Stable Transfection, Comparison, Plasmid Preparation, Control, Imaging, Immunohistochemistry

Journal: Hepatobiliary Surgery and Nutrition

Article Title: Choline suppresses hepatocellular carcinoma progression by attenuating AMPK/mTOR-mediated autophagy via choline transporter SLC5A7 activation

doi: 10.21037/hbsn-22-476

Figure Lengend Snippet: Choline inhibits autophagy through SLC5A7/AMPK/mTOR pathway. (A) WB analysis of total p53 and cytoplasm p53 in HCC cells treated with choline. Expression of total p53 and cytoplasm p53 was quantitated based on optical density. The level of total p53 and cytoplasm p53 was normalized to ACTB levels of the same lane. (B) WB analysis of total p53 and cytoplasm p53 in HCC cells treated with choline alone and combined with si-SLC5A7. Expression of total p53 and cytoplasm p53 was quantitated based on optical density. The level of total p53 and cytoplasm p53 was normalized to ACTB levels of the same lane. (C,D) WB analysis of p-AMPK, total AMPK, p-mTOR, total mTOR, p-P70S6K, total P70S6K, p-4EBP1, and total 4EBP1 in HCC cells and HUH-7 xenograft tissues with choline treatment. Expression of p-AMPK, total AMPK, p-mTOR, total mTOR, p-P70S6K, total P70S6K, p-4EBP1, and total 4EBP1 was quantitated based on optical density and normalized to ACTB levels of the same lane. (E) WB analysis of p-AMPK, total AMPK, p-mTOR, total mTOR, p-P70S6K, total P70S6K, p-4EBP1, and total 4EBP1 in HCC cells with choline alone and choline combined with si-SLC5A7. Expression of p-AMPK, total AMPK, p-mTOR, total mTOR, p-P70S6K, total P70S6K, p-4EBP1, and total 4EBP1, was quantified based on optical density and normalized to ACTB levels of the same lane. (F) HUH-7 and Hep3B cells were subjected to four groups: Control group, Choline group, Choline + si-SLC5A7 group, and Choline + si-SLC5A7 + si-AMPK group. WB analysis of p-AMPK, total AMPK, p-mTOR, total mTOR, p-P70S6K, total P70S6K, p-4EBP1, total 4EBP1, and LC3 in HCC cells. Expression of p-AMPK, total AMPK, p-mTOR, total mTOR, p-P70S6K, total P70S6K, p-4EBP1, total 4EBP1, and LC3 was quantitated based on optical density and normalized to ACTB levels of the same lane. Choline group vs. control group: *, P<0.05; **, P<0.01; ***, P<0.001. Choline + si-SLC5A7 group vs. choline group: # , P<0.05; ## , P<0.01; ### , P<0.001. Choline + si-SLC5A7 + si-AMPK group vs. choline + si-SLC5A7 group: & , P<0.05; && , P<0.01; &&& , P<0.001. ACTB, beta-actin; WB, Western blotting; HCC, hepatocellular carcinoma.

Article Snippet: Primary antibodies of ACTB, LC3B, SQSTM1/p62, BECN1, SLC5A7, p53, AMPK, p-AMPK, and p-P70S6K were purchased from Abclonal (Wuhan, China).

Techniques: Expressing, Control, Western Blot

Journal: Frontiers in Cellular Neuroscience

Article Title: Creatine in the fetal brain: A regional investigation of acute global hypoxia and creatine supplementation in a translational fetal sheep model

doi: 10.3389/fncel.2023.1154772

Figure Lengend Snippet: Genes of interest.

Article Snippet: , Choline transporter , SLC5A7 , Oa03234527_m1.

Techniques: