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ska121  (MedChemExpress)


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    MedChemExpress ska121
    Effects of K Ca 3.1 activation and LRRC8A inhibition on CCL2 expression and secretion in M 2 -MACs. ( A , B ): Real-time PCR examination of CCL22 ( A ) and CCL2 ( B ) expression in native THP-1 cells (native) and M 2 -MACs (M 2 ) ( n = 4). Data are normalized to ACTB. ( C , D , F , G ): Real-time PCR examination of CCL22 ( C , F ) and CCL2 ( D , G ) expression following treatment with vehicle, 10 μM <t>SKA121</t> (SKA) ( C , D ), or 10 μM endovion (EDV) ( F , G ) for 12 h in M 2 -MACs ( n = 4). Expression levels are normalized to ACTB and shown relative to vehicle control (set as 1.0). ( E , H ): ELISA quantification of CCL2 secretion after 24 h treatment with SKA ( E ) or EDV ( H ) in M 2 -MACs ( n = 4). Secretion levels are shown relative to vehicle control (set as 1.0). **: p < 0.01 vs. native THP-1 or vehicle control.
    Ska121, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ska121/SKA-121/pmc12347553-203-2-11
    Average 93 stars, based on 3 article reviews
    ska121 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages"

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26157624

    Effects of K Ca 3.1 activation and LRRC8A inhibition on CCL2 expression and secretion in M 2 -MACs. ( A , B ): Real-time PCR examination of CCL22 ( A ) and CCL2 ( B ) expression in native THP-1 cells (native) and M 2 -MACs (M 2 ) ( n = 4). Data are normalized to ACTB. ( C , D , F , G ): Real-time PCR examination of CCL22 ( C , F ) and CCL2 ( D , G ) expression following treatment with vehicle, 10 μM SKA121 (SKA) ( C , D ), or 10 μM endovion (EDV) ( F , G ) for 12 h in M 2 -MACs ( n = 4). Expression levels are normalized to ACTB and shown relative to vehicle control (set as 1.0). ( E , H ): ELISA quantification of CCL2 secretion after 24 h treatment with SKA ( E ) or EDV ( H ) in M 2 -MACs ( n = 4). Secretion levels are shown relative to vehicle control (set as 1.0). **: p < 0.01 vs. native THP-1 or vehicle control.
    Figure Legend Snippet: Effects of K Ca 3.1 activation and LRRC8A inhibition on CCL2 expression and secretion in M 2 -MACs. ( A , B ): Real-time PCR examination of CCL22 ( A ) and CCL2 ( B ) expression in native THP-1 cells (native) and M 2 -MACs (M 2 ) ( n = 4). Data are normalized to ACTB. ( C , D , F , G ): Real-time PCR examination of CCL22 ( C , F ) and CCL2 ( D , G ) expression following treatment with vehicle, 10 μM SKA121 (SKA) ( C , D ), or 10 μM endovion (EDV) ( F , G ) for 12 h in M 2 -MACs ( n = 4). Expression levels are normalized to ACTB and shown relative to vehicle control (set as 1.0). ( E , H ): ELISA quantification of CCL2 secretion after 24 h treatment with SKA ( E ) or EDV ( H ) in M 2 -MACs ( n = 4). Secretion levels are shown relative to vehicle control (set as 1.0). **: p < 0.01 vs. native THP-1 or vehicle control.

    Techniques Used: Activation Assay, Inhibition, Expressing, Real-time Polymerase Chain Reaction, Control, Enzyme-linked Immunosorbent Assay

    Related Articles

    Activation Assay:

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages
    Article Snippet: Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).. EDV (HY-105917), SKA121 (HY-107414), SP600125 (HY-12041), and PD169316 (HY-10578) were from MedChemExpress (Monmouth Junction, NJ, USA).. Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).

    Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages
    Article Snippet: The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).. EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).. IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific (Waltham, MA USA). siRNAs for control-A (sc-37007) and HDAC3 (sc-35538) were from Santa Cruz Biotechnology, Sant Cruz, CA, USA.IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific..

    Inhibition:

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages
    Article Snippet: Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).. EDV (HY-105917), SKA121 (HY-107414), SP600125 (HY-12041), and PD169316 (HY-10578) were from MedChemExpress (Monmouth Junction, NJ, USA).. Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).

    Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages
    Article Snippet: The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).. EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).. IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific (Waltham, MA USA). siRNAs for control-A (sc-37007) and HDAC3 (sc-35538) were from Santa Cruz Biotechnology, Sant Cruz, CA, USA.IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific..

    Expressing:

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages
    Article Snippet: Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).. EDV (HY-105917), SKA121 (HY-107414), SP600125 (HY-12041), and PD169316 (HY-10578) were from MedChemExpress (Monmouth Junction, NJ, USA).. Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).

    Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages
    Article Snippet: The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).. EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).. IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific (Waltham, MA USA). siRNAs for control-A (sc-37007) and HDAC3 (sc-35538) were from Santa Cruz Biotechnology, Sant Cruz, CA, USA.IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific..

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages
    Article Snippet: Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).. EDV (HY-105917), SKA121 (HY-107414), SP600125 (HY-12041), and PD169316 (HY-10578) were from MedChemExpress (Monmouth Junction, NJ, USA).. Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).

    Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages
    Article Snippet: The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).. EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).. IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific (Waltham, MA USA). siRNAs for control-A (sc-37007) and HDAC3 (sc-35538) were from Santa Cruz Biotechnology, Sant Cruz, CA, USA.IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific..

    Control:

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages
    Article Snippet: Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).. EDV (HY-105917), SKA121 (HY-107414), SP600125 (HY-12041), and PD169316 (HY-10578) were from MedChemExpress (Monmouth Junction, NJ, USA).. Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).

    Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages
    Article Snippet: The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).. EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).. IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific (Waltham, MA USA). siRNAs for control-A (sc-37007) and HDAC3 (sc-35538) were from Santa Cruz Biotechnology, Sant Cruz, CA, USA.IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages
    Article Snippet: Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).Luna Universal qPCR Master Mix (M3003E) was from New England Biolabs Japan (Tokyo, Japan).. EDV (HY-105917), SKA121 (HY-107414), SP600125 (HY-12041), and PD169316 (HY-10578) were from MedChemExpress (Monmouth Junction, NJ, USA).. Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).Human MCP-1/CCL2 Uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7399-88), Human IL-10/IL-8 Uncoated ELISA kits (88-7106-77, 88-8086-77), pre-designed siRNAs for CEBPB (siRNA ID: s2893), LRRC8A (s32108), MAGT1 (s224928), RUNX1 (s229351), Silencer Select Negative Control No. 1 siRNA (4390843), and SuperSignal West Pico PLUS Chemiluminescent Substrate (34580) were from Thermo Fisher Scientific (Waltham, MA USA).

    Article Title: Downregulation of IL-8 and IL-10 by LRRC8A Inhibition through the NOX2–Nrf2–CEBPB Transcriptional Axis in THP-1-Derived M 2 Macrophages
    Article Snippet: The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).The ECL Advance Chemiluminescence Kit (02230-30) was from Nacalai Tesque (Kyoto, Japan).. EDV (HY-105917), ANO1-IN-1 (HY-16320), NK252 (HY-19734), SU056 (HY-50231), and SKA121 (HY-107414) were from MedChemExpress (Monmouth Junction, NJ, USA).. IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific (Waltham, MA USA). siRNAs for control-A (sc-37007) and HDAC3 (sc-35538) were from Santa Cruz Biotechnology, Sant Cruz, CA, USA.IL-10/IL-8 human uncoated enzyme-linked immunosorbent assay (ELISA) kits (88-7106-77, 88-8086-77), the Halt TM phosphatase inhibitor cocktail (SF-78420), and validated/pre-designed siRNAs for LRRC8A (siRNA ID: s32107, pre-designed), Nrf2 (s9491, pre-designed), VDAC1 (s14768, validated), CLIC1 (s636, validated), ANO6 (s225825, pre-designed), CLCN3 (s3136, validated), CEBPB (s2892, pre-designed), NSUN2 (s29683, pre-designed), and WNK1 (s35234, validated) were from Thermo Fisher Scientific..



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    ska121  (MedChemExpress)
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    MedChemExpress ska121
    Effects of K Ca 3.1 activation and LRRC8A inhibition on CCL2 expression and secretion in M 2 -MACs. ( A , B ): Real-time PCR examination of CCL22 ( A ) and CCL2 ( B ) expression in native THP-1 cells (native) and M 2 -MACs (M 2 ) ( n = 4). Data are normalized to ACTB. ( C , D , F , G ): Real-time PCR examination of CCL22 ( C , F ) and CCL2 ( D , G ) expression following treatment with vehicle, 10 μM <t>SKA121</t> (SKA) ( C , D ), or 10 μM endovion (EDV) ( F , G ) for 12 h in M 2 -MACs ( n = 4). Expression levels are normalized to ACTB and shown relative to vehicle control (set as 1.0). ( E , H ): ELISA quantification of CCL2 secretion after 24 h treatment with SKA ( E ) or EDV ( H ) in M 2 -MACs ( n = 4). Secretion levels are shown relative to vehicle control (set as 1.0). **: p < 0.01 vs. native THP-1 or vehicle control.
    Ska121, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ska121/SKA-121/pmc12347553-203-2-11
    Average 93 stars, based on 1 article reviews
    ska121 - by Bioz Stars, 2026-09
    93/100 stars
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    Image Search Results


    Effects of K Ca 3.1 activation and LRRC8A inhibition on CCL2 expression and secretion in M 2 -MACs. ( A , B ): Real-time PCR examination of CCL22 ( A ) and CCL2 ( B ) expression in native THP-1 cells (native) and M 2 -MACs (M 2 ) ( n = 4). Data are normalized to ACTB. ( C , D , F , G ): Real-time PCR examination of CCL22 ( C , F ) and CCL2 ( D , G ) expression following treatment with vehicle, 10 μM SKA121 (SKA) ( C , D ), or 10 μM endovion (EDV) ( F , G ) for 12 h in M 2 -MACs ( n = 4). Expression levels are normalized to ACTB and shown relative to vehicle control (set as 1.0). ( E , H ): ELISA quantification of CCL2 secretion after 24 h treatment with SKA ( E ) or EDV ( H ) in M 2 -MACs ( n = 4). Secretion levels are shown relative to vehicle control (set as 1.0). **: p < 0.01 vs. native THP-1 or vehicle control.

    Journal: International Journal of Molecular Sciences

    Article Title: Transcriptional Repression of CCL2 by K Ca 3.1 K + Channel Activation and LRRC8A Anion Channel Inhibition in THP-1-Differentiated M 2 Macrophages

    doi: 10.3390/ijms26157624

    Figure Lengend Snippet: Effects of K Ca 3.1 activation and LRRC8A inhibition on CCL2 expression and secretion in M 2 -MACs. ( A , B ): Real-time PCR examination of CCL22 ( A ) and CCL2 ( B ) expression in native THP-1 cells (native) and M 2 -MACs (M 2 ) ( n = 4). Data are normalized to ACTB. ( C , D , F , G ): Real-time PCR examination of CCL22 ( C , F ) and CCL2 ( D , G ) expression following treatment with vehicle, 10 μM SKA121 (SKA) ( C , D ), or 10 μM endovion (EDV) ( F , G ) for 12 h in M 2 -MACs ( n = 4). Expression levels are normalized to ACTB and shown relative to vehicle control (set as 1.0). ( E , H ): ELISA quantification of CCL2 secretion after 24 h treatment with SKA ( E ) or EDV ( H ) in M 2 -MACs ( n = 4). Secretion levels are shown relative to vehicle control (set as 1.0). **: p < 0.01 vs. native THP-1 or vehicle control.

    Article Snippet: EDV (HY-105917), SKA121 (HY-107414), SP600125 (HY-12041), and PD169316 (HY-10578) were from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Activation Assay, Inhibition, Expressing, Real-time Polymerase Chain Reaction, Control, Enzyme-linked Immunosorbent Assay