anti sirt2 antibody proteintech (Proteintech)

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Proteintech anti sirt2 antibody proteintech
Anti Sirt2 Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sirt2/SIRT2+Antibody/pm41932528-253-0-2
Average 95 stars, based on 59 article reviews

anti sirt2 antibody proteintech - by Bioz Stars, 2026-09

95/100 stars

Images

Western Blot:

Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function
Article Snippet: .. The primary antibodies used for Western blotting included: p53 (Wanleibio, WL01919), p27 (Wanleibio, WL04174), p21 (Wanleibio, WL0362), p16 (Wanleibio, WL01418), p‐tau (Abclonal, AP1096), tau‐5 (Affinity, AF6141), NEFL (Proteintech, 12998‐1‐AP), SIRT2 (Proteintech, 19655‐1‐AP), GH1 (Proteintech, 55243‐1‐AP), IGF (Abclonal, A11985), and β‐actin (Wanleibio, WL01372). .. Rat bone marrow‐derived mesenchymal stem cells (BMSCs) were isolated and cultured in Dulbecco's Modified Eagle's Medium (DMEM, Servicebio, G4510) supplemented with 1% fetal bovine serum (FBS, TIANHANG, 11011‐8611) at 37°C in a 5% CO 2 atmosphere.

Article Title: SIRT2 Mitigates Radiation-Induced Oral Mucositis by Promoting Homologous Recombination-Mediated DNA Double-Strand Break Repair in Epithelial Stem Cells
Article Snippet: Fluorescence images were visualized and captured using Zeiss LSM 800 confocal microscope and analyzed via ImageJ. .. The following antibodies were used: SIRT2 (15345-1-AP, Proteintech; 1/4000 for WB, 1/1000 for IHC and 2ug/mg lysate for IP), acetyl-tubulin (66200-1-Ig, Proteintech, 1/2000 for WB and 1/1000 for IHC), P63 (sc-25268, Santa Cruz Biotechnologies, 1/500 for IHC), GAPDH (60004-1-Ig, Proteintech, 1/10000 for WB), Rad51 (ab133534, Abcam, 1/500 for IHC), p-H2AX (ab11174, Abcam, 1/1000 for IHC), CtIP (sc-271339, Santa Cruz Biotechnologies, 1/500 for WB and 5ug/mg lysate for IP), SIRT1 (8469, CST, 1/500 for IHC), IL-6 (AMC0862, Invitrogen, 1/500 for IHC), TNF-α(554640, BD Biosciences, 1/200 for IHC), and NRF2 (PA5-27882, Invitrogen, 1/400 for IHC). .. The secondary antibodies used were: AF-488 Goat anti-Mouse IgG (A-1100, Invitrogen, 1/2000 for IF), AF-594 Goat anti-Rabbit IgG (A-11012, Ivitrogen, 1/2000 for IF), HRP-Anti-mouse IgG (7076, Cell Signaling Technology, 1/5000 for WB), HRP-Anti-rabbit IgG (7074, Cell Signaling Technology; 1/5000 for WB), and IHC staining kit (ab64264, Abcam).

Article Title: SIRT2 Mitigates Radiation-Induced Oral Mucositis by Promoting Homologous Recombination-Mediated DNA Double-Strand Break Repair in Epithelial Stem Cells.
Article Snippet: Radiation-induced oral mucositis (RIOM) is a common and debilitating toxicity of radiotherapy for head and neck squamous cell carcinoma (HNSCC), often compromising treatment adherence and profoundly impairing patient quality of life.. Despite its significant clinical impact, there are currently no safe and well-tolerated FDA-approved therapies to prevent or treat RIOM.. The underlying mechanisms remain poorly understood but are thought to involve inflammatory responses triggered by oxidative stress and DNA damage from ionizing radiation (IR).

other:

Article Title: Lactylation Enhances the Activity of Lactate Dehydrogenase A and Promotes the Chemoresistance to Cisplatin Through Facilitating DNA Nonhomologous End Junction in Lung Adenocarcinoma
Article Snippet: Antibodies: Kla (1:1000, PTM BIO Cat# PTM‐1401RM, RRID:AB_2 942 013), LDHA (1:2000, Proteintech Cat# 19987‐1‐AP, RRID:AB_10 646 429), LDHB (1:2000, Proteintech Cat# 14824‐1‐AP, RRID:AB_2 134 953), p300 (1:1000, Proteintech Cat# 20695‐1‐AP, RRID:AB_3 085 614) AARS1 (1:1000, Proteintech Cat# 67909‐1‐Ig, RRID:AB_2 918 664), SIRT2 (1:1000, Proteintech Cat# 66410‐1‐Ig, RRID:AB_2 881 782), HA (1:1000, Thermo Fisher Scientific Cat# 26183‐D550, RRID:AB_2 533 052), Flag (1:1000, Proteintech Cat# 66008‐4‐Ig, RRID:AB_2 918 475), MYC (1:1000, Proteintech Cat# 60003‐2‐Ig, RRID:AB_2 734 122), H2A.X (1:1000, Proteintech Cat# 10856‐1‐AP, RRID:AB_2 114 985), γ‐H2AX (1:1000, Proteintech Cat# 29380‐1‐AP, RRID:AB_3 085 345) and β‐actin (1:3000, Proteintech Cat# 60008‐1‐Ig, RRID:AB_2 289 225).

Immunohistochemistry:

Article Title: SIRT2 Mitigates Radiation-Induced Oral Mucositis by Promoting Homologous Recombination-Mediated DNA Double-Strand Break Repair in Epithelial Stem Cells.
Article Snippet: Radiation-induced oral mucositis (RIOM) is a common and debilitating toxicity of radiotherapy for head and neck squamous cell carcinoma (HNSCC), often compromising treatment adherence and profoundly impairing patient quality of life.. Despite its significant clinical impact, there are currently no safe and well-tolerated FDA-approved therapies to prevent or treat RIOM.. The underlying mechanisms remain poorly understood but are thought to involve inflammatory responses triggered by oxidative stress and DNA damage from ionizing radiation (IR).

Pyrolysis Gas Chromatography:

Article Title: ASIC1a Promotes nucleus pulposus derived stem cells apoptosis through modulation of SIRT3-dependent mitochondrial redox homeostasis in intervertebral disc degeneration
Article Snippet: Following electrophoresis, proteins were transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA). .. The primary antibodies utilized included ASIC1a (Huaan ER1803-33, 1:1000), SIRT1 (Huaan ER130811 , 1:1000), SIRT2 (Huaan ET1611-72, 1:1000), SIRT3 (Immunoway YT4304, 1:1000), SIRT4 (Proteintech 66543-ig, 1:20000), SIRT5 (Huaan ET1701-6, 1:1000), SIRT6 (Huaan ET1701-29, 1:1000), SIRT7 (Huaan EM1707-09, 1:1000), BAX (Proteintech 50599-2-ig, 1:5000), Bcl-2 (Proteintech 26593-1-AP, 1:2000), Active Caspase-3 (Huaan ET1602-47, 1:1000), Cytochrome C (Huaan ET1610-16, 1:1000), SOD2 (Immunoway YT5575, 1:1000), AMPK (Huaan ET1608-40, 1:4000), p-AMPK (Huaan ET1612-72, 1:1000), PGC-1α (Huaan ET1702-96, 1:1000), VDAC (Huaan ET1601-20, 1:10000), and Beta Actin (Proteintech 20536-1-AP, 1:10000). .. After incubation with the corresponding secondary antibodies and subsequent washing, BeyoECL Plus (Beyotime P0018s) was employed to develop luminescence, which was detected using an automatic chemiluminescence immunoanalyzer (Tanon 5200, China).

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