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sirt2 inhibitor agk2  (MedChemExpress)


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    MedChemExpress sirt2 inhibitor agk2
    Sirt2 Inhibitor Agk2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sirt2/PKM2-IN-1/pm42034028-85-26-31
    Average 95 stars, based on 38 article reviews
    sirt2 inhibitor agk2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. RNA was analyzed by RNA sequencing to compare the expression profiles of the control and virus-infected cells from lung with certain genes involved in ROS, cytokine and chemokine signaling pathways. (B-E) Pad4, Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Sirt2-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Expressing:

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. RNA was analyzed by RNA sequencing to compare the expression profiles of the control and virus-infected cells from lung with certain genes involved in ROS, cytokine and chemokine signaling pathways. (B-E) Pad4, Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Sirt2-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: .. Mass spectrometry analysis of the metabolite abundance of 3-HA in neutrophils from BALF and in lung tissue from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (C) Mass spectrometry analysis of the metabolite abundance of 3-HA in Epcam1 + and Epicam1 - cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (D) Flow cytometry analysis of TDO expression in neutrophils and epithelial cells in the lungs of WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) Ido1/2 and Tdo mRNA expression of neutrophils or cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (F–H) Neutrophils were isolated from the spleens of WT and Sirt2 −/− mice cocultured with vehicle (in PBS), epithelial cells or epithelial cells treated with a TDO inhibitor (LM10, 10 nM, MCE) and stimulated with virus for different durations. ..

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. Expression of ROS in neutrophils isolated from BALF by flow cytometry and data summarized. (B-C) Pad4 mRNA (B) and protein (C) expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). (D-F) Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Control:

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. RNA was analyzed by RNA sequencing to compare the expression profiles of the control and virus-infected cells from lung with certain genes involved in ROS, cytokine and chemokine signaling pathways. (B-E) Pad4, Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Sirt2-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Article Title: SIRT2 Delays Vulnerable Plaque Progression by Modulating Vascular Smooth Muscle Cell Senescence.
    Article Snippet: .. To investigate the role and potential mechanism of Sirt2 in VSMC senescence, the VSMCs were divided into the following six groups: (1) Control group; (2) H2O2 group: treated with 10 μM H2O2 for 72 hours; (3) AGK2 group: treated with 10 μM H2O2 and 10 μM AGK2 (a selective Sirt2 inhibitor, MCE, HY-100578, USA) for 72 hours; (4) LV-NC group: VSMCs infected with empty lentivirus to obtain stable transfectants, then treated with 10 μM H2O2 for 72 hours; (5) LV-Sirt2 group: VSMCs stably overexpressing Sirt2 treated with 10 μM H2O2 for 72 hours; (6) BAY-3827 group: VSMCs stably overexpressing Sirt2 treated with 10 μM H2O2 and 10 nM BAY-3827 (a selective AMPK inhibitor, MCE, HY112083, USA) for 72 hours. .. To investigate the potential of Sirt2-targeting agents in VSMC senescence, we performed experiments using resveratrol (MCE, HY-16561, USA).

    Virus:

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. RNA was analyzed by RNA sequencing to compare the expression profiles of the control and virus-infected cells from lung with certain genes involved in ROS, cytokine and chemokine signaling pathways. (B-E) Pad4, Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Sirt2-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: QPRT is expressed in neutrophils and is responsible for NAD + synthesis induced by Sirt2 −/− (A and B) WT and Sirt2 −/− mice were challenged with the PR8 virus at 48 h. Qprt mRNA (A) and 3-HAO mRNA expression in neutrophils isolated from BALF in mice. (C) Qprt mRNA expression in neutrophils isolated from mouse spleens and stimulated for 24 h with 1 μg/mL LPS, 30 μg/mL Poly I:C and 5 μg/mL R848 (all from Sigma). (D) Western blot of QPRT and 3-HAO in neutrophils from BALF from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) QPRT and 3-HAO for the NAD + synthesis pathway. (F–H) Neutrophils isolated from the spleens of WT and Sirt2 −/− mice and transfected with lentivirus containing control vector small interfering RNA (siRNA ctrl) or the Qprt gene (siRNA Qprt) were stimulated for 24 h by the virus. .. Changes in the NAD + level (F), percent of NET + cells (G) and intracellular staining for TNF-α (H) in Ly6G + neutrophils transfected with siCtrl and siQprt. (I–L) CD11b + Ly6G + neutrophils were isolated from the spleens of WT and Sirt2 −/− mice and stimulated for 24 h with virus in the presence of 3-hydroxyanthranilate 3,4-dioxygenase (3-HAO, 2 nM, MCE). ..

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. Wild-type (WT) and Sirt2-/- mice infected by PR8 virus for 48 hours and treated with or without Yoda1 (2.6 mg/kg, MCE). ..

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: .. Mass spectrometry analysis of the metabolite abundance of 3-HA in neutrophils from BALF and in lung tissue from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (C) Mass spectrometry analysis of the metabolite abundance of 3-HA in Epcam1 + and Epicam1 - cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (D) Flow cytometry analysis of TDO expression in neutrophils and epithelial cells in the lungs of WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) Ido1/2 and Tdo mRNA expression of neutrophils or cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (F–H) Neutrophils were isolated from the spleens of WT and Sirt2 −/− mice cocultured with vehicle (in PBS), epithelial cells or epithelial cells treated with a TDO inhibitor (LM10, 10 nM, MCE) and stimulated with virus for different durations. ..

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. Expression of ROS in neutrophils isolated from BALF by flow cytometry and data summarized. (B-C) Pad4 mRNA (B) and protein (C) expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). (D-F) Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Protein-Protein interactions:

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. RNA was analyzed by RNA sequencing to compare the expression profiles of the control and virus-infected cells from lung with certain genes involved in ROS, cytokine and chemokine signaling pathways. (B-E) Pad4, Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Sirt2-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Infection:

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. RNA was analyzed by RNA sequencing to compare the expression profiles of the control and virus-infected cells from lung with certain genes involved in ROS, cytokine and chemokine signaling pathways. (B-E) Pad4, Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Sirt2-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. Wild-type (WT) and Sirt2-/- mice infected by PR8 virus for 48 hours and treated with or without Yoda1 (2.6 mg/kg, MCE). ..

    Article Title: SIRT2 Delays Vulnerable Plaque Progression by Modulating Vascular Smooth Muscle Cell Senescence.
    Article Snippet: .. To investigate the role and potential mechanism of Sirt2 in VSMC senescence, the VSMCs were divided into the following six groups: (1) Control group; (2) H2O2 group: treated with 10 μM H2O2 for 72 hours; (3) AGK2 group: treated with 10 μM H2O2 and 10 μM AGK2 (a selective Sirt2 inhibitor, MCE, HY-100578, USA) for 72 hours; (4) LV-NC group: VSMCs infected with empty lentivirus to obtain stable transfectants, then treated with 10 μM H2O2 for 72 hours; (5) LV-Sirt2 group: VSMCs stably overexpressing Sirt2 treated with 10 μM H2O2 for 72 hours; (6) BAY-3827 group: VSMCs stably overexpressing Sirt2 treated with 10 μM H2O2 and 10 nM BAY-3827 (a selective AMPK inhibitor, MCE, HY112083, USA) for 72 hours. .. To investigate the potential of Sirt2-targeting agents in VSMC senescence, we performed experiments using resveratrol (MCE, HY-16561, USA).

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: .. Mass spectrometry analysis of the metabolite abundance of 3-HA in neutrophils from BALF and in lung tissue from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (C) Mass spectrometry analysis of the metabolite abundance of 3-HA in Epcam1 + and Epicam1 - cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (D) Flow cytometry analysis of TDO expression in neutrophils and epithelial cells in the lungs of WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) Ido1/2 and Tdo mRNA expression of neutrophils or cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (F–H) Neutrophils were isolated from the spleens of WT and Sirt2 −/− mice cocultured with vehicle (in PBS), epithelial cells or epithelial cells treated with a TDO inhibitor (LM10, 10 nM, MCE) and stimulated with virus for different durations. ..

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. Expression of ROS in neutrophils isolated from BALF by flow cytometry and data summarized. (B-C) Pad4 mRNA (B) and protein (C) expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). (D-F) Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Staining:

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: QPRT is expressed in neutrophils and is responsible for NAD + synthesis induced by Sirt2 −/− (A and B) WT and Sirt2 −/− mice were challenged with the PR8 virus at 48 h. Qprt mRNA (A) and 3-HAO mRNA expression in neutrophils isolated from BALF in mice. (C) Qprt mRNA expression in neutrophils isolated from mouse spleens and stimulated for 24 h with 1 μg/mL LPS, 30 μg/mL Poly I:C and 5 μg/mL R848 (all from Sigma). (D) Western blot of QPRT and 3-HAO in neutrophils from BALF from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) QPRT and 3-HAO for the NAD + synthesis pathway. (F–H) Neutrophils isolated from the spleens of WT and Sirt2 −/− mice and transfected with lentivirus containing control vector small interfering RNA (siRNA ctrl) or the Qprt gene (siRNA Qprt) were stimulated for 24 h by the virus. .. Changes in the NAD + level (F), percent of NET + cells (G) and intracellular staining for TNF-α (H) in Ly6G + neutrophils transfected with siCtrl and siQprt. (I–L) CD11b + Ly6G + neutrophils were isolated from the spleens of WT and Sirt2 −/− mice and stimulated for 24 h with virus in the presence of 3-hydroxyanthranilate 3,4-dioxygenase (3-HAO, 2 nM, MCE). ..

    Transfection:

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: QPRT is expressed in neutrophils and is responsible for NAD + synthesis induced by Sirt2 −/− (A and B) WT and Sirt2 −/− mice were challenged with the PR8 virus at 48 h. Qprt mRNA (A) and 3-HAO mRNA expression in neutrophils isolated from BALF in mice. (C) Qprt mRNA expression in neutrophils isolated from mouse spleens and stimulated for 24 h with 1 μg/mL LPS, 30 μg/mL Poly I:C and 5 μg/mL R848 (all from Sigma). (D) Western blot of QPRT and 3-HAO in neutrophils from BALF from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) QPRT and 3-HAO for the NAD + synthesis pathway. (F–H) Neutrophils isolated from the spleens of WT and Sirt2 −/− mice and transfected with lentivirus containing control vector small interfering RNA (siRNA ctrl) or the Qprt gene (siRNA Qprt) were stimulated for 24 h by the virus. .. Changes in the NAD + level (F), percent of NET + cells (G) and intracellular staining for TNF-α (H) in Ly6G + neutrophils transfected with siCtrl and siQprt. (I–L) CD11b + Ly6G + neutrophils were isolated from the spleens of WT and Sirt2 −/− mice and stimulated for 24 h with virus in the presence of 3-hydroxyanthranilate 3,4-dioxygenase (3-HAO, 2 nM, MCE). ..

    Isolation:

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: QPRT is expressed in neutrophils and is responsible for NAD + synthesis induced by Sirt2 −/− (A and B) WT and Sirt2 −/− mice were challenged with the PR8 virus at 48 h. Qprt mRNA (A) and 3-HAO mRNA expression in neutrophils isolated from BALF in mice. (C) Qprt mRNA expression in neutrophils isolated from mouse spleens and stimulated for 24 h with 1 μg/mL LPS, 30 μg/mL Poly I:C and 5 μg/mL R848 (all from Sigma). (D) Western blot of QPRT and 3-HAO in neutrophils from BALF from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) QPRT and 3-HAO for the NAD + synthesis pathway. (F–H) Neutrophils isolated from the spleens of WT and Sirt2 −/− mice and transfected with lentivirus containing control vector small interfering RNA (siRNA ctrl) or the Qprt gene (siRNA Qprt) were stimulated for 24 h by the virus. .. Changes in the NAD + level (F), percent of NET + cells (G) and intracellular staining for TNF-α (H) in Ly6G + neutrophils transfected with siCtrl and siQprt. (I–L) CD11b + Ly6G + neutrophils were isolated from the spleens of WT and Sirt2 −/− mice and stimulated for 24 h with virus in the presence of 3-hydroxyanthranilate 3,4-dioxygenase (3-HAO, 2 nM, MCE). ..

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: .. Mass spectrometry analysis of the metabolite abundance of 3-HA in neutrophils from BALF and in lung tissue from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (C) Mass spectrometry analysis of the metabolite abundance of 3-HA in Epcam1 + and Epicam1 - cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (D) Flow cytometry analysis of TDO expression in neutrophils and epithelial cells in the lungs of WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) Ido1/2 and Tdo mRNA expression of neutrophils or cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (F–H) Neutrophils were isolated from the spleens of WT and Sirt2 −/− mice cocultured with vehicle (in PBS), epithelial cells or epithelial cells treated with a TDO inhibitor (LM10, 10 nM, MCE) and stimulated with virus for different durations. ..

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. Expression of ROS in neutrophils isolated from BALF by flow cytometry and data summarized. (B-C) Pad4 mRNA (B) and protein (C) expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). (D-F) Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..

    Stable Transfection:

    Article Title: SIRT2 Delays Vulnerable Plaque Progression by Modulating Vascular Smooth Muscle Cell Senescence.
    Article Snippet: .. To investigate the role and potential mechanism of Sirt2 in VSMC senescence, the VSMCs were divided into the following six groups: (1) Control group; (2) H2O2 group: treated with 10 μM H2O2 for 72 hours; (3) AGK2 group: treated with 10 μM H2O2 and 10 μM AGK2 (a selective Sirt2 inhibitor, MCE, HY-100578, USA) for 72 hours; (4) LV-NC group: VSMCs infected with empty lentivirus to obtain stable transfectants, then treated with 10 μM H2O2 for 72 hours; (5) LV-Sirt2 group: VSMCs stably overexpressing Sirt2 treated with 10 μM H2O2 for 72 hours; (6) BAY-3827 group: VSMCs stably overexpressing Sirt2 treated with 10 μM H2O2 and 10 nM BAY-3827 (a selective AMPK inhibitor, MCE, HY112083, USA) for 72 hours. .. To investigate the potential of Sirt2-targeting agents in VSMC senescence, we performed experiments using resveratrol (MCE, HY-16561, USA).

    Mass Spectrometry:

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: .. Mass spectrometry analysis of the metabolite abundance of 3-HA in neutrophils from BALF and in lung tissue from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (C) Mass spectrometry analysis of the metabolite abundance of 3-HA in Epcam1 + and Epicam1 - cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (D) Flow cytometry analysis of TDO expression in neutrophils and epithelial cells in the lungs of WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) Ido1/2 and Tdo mRNA expression of neutrophils or cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (F–H) Neutrophils were isolated from the spleens of WT and Sirt2 −/− mice cocultured with vehicle (in PBS), epithelial cells or epithelial cells treated with a TDO inhibitor (LM10, 10 nM, MCE) and stimulated with virus for different durations. ..

    Flow Cytometry:

    Article Title: Sirtuin 2 regulates neutrophil functions through NAD + synthesis pathway in virus infection
    Article Snippet: .. Mass spectrometry analysis of the metabolite abundance of 3-HA in neutrophils from BALF and in lung tissue from WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (C) Mass spectrometry analysis of the metabolite abundance of 3-HA in Epcam1 + and Epicam1 - cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (D) Flow cytometry analysis of TDO expression in neutrophils and epithelial cells in the lungs of WT and Sirt2 −/− mice challenged with the PR8 virus at 48 h. (E) Ido1/2 and Tdo mRNA expression of neutrophils or cells from lung tissue from WT and Sirt2 −/− mice challenged by PR8 virus infection at 48 h. (F–H) Neutrophils were isolated from the spleens of WT and Sirt2 −/− mice cocultured with vehicle (in PBS), epithelial cells or epithelial cells treated with a TDO inhibitor (LM10, 10 nM, MCE) and stimulated with virus for different durations. ..

    Article Title: Piezo1-directed neutrophil extracellular traps regulate macrophage differentiation during influenza virus infection
    Article Snippet: .. Expression of ROS in neutrophils isolated from BALF by flow cytometry and data summarized. (B-C) Pad4 mRNA (B) and protein (C) expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). (D-F) Piezo1, Sirt2 and Hif1α mRNA expression in sorted neutrophils from BALF from virus infected WT and Hif1α-/- mice treatment with or without Yoda1 (2.6 mg/kg, MCE). ..



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