oe sirt2 skl2001  (MedChemExpress)

Journal: Journal of Neuroinflammation

Article Title: SIRT2 Inhibition promotes microglia LC3-associated phagocytosis via NRF2/CD36 after the experimental subarachnoid hemorrhage

doi: 10.1186/s12974-025-03623-z

Figure Lengend Snippet: Expression of SIRT2 and its prognostic impact after SAH in rats. A-B, Western blot and quantifications of SIRT2 level at different time courses. C-E, Representative images show in the temporal cortex stained with antibodies to SIRT2 and Iba1 (C), NeuN (D), or GFAP (E) at 24 h post-SAH. F, The mean fluorescence intensity (MFI) of SIRT2 immunoreactivity. G, Percentages of Iba1 + SIRT2 + , NeuN + SIRT2 + and GFAP + SIRT2 + in the temporal cortex of each group. H , Neurological score, I , brain water content ( J ) and Evan’s blue in different (Sham, SAH, Vehicle and AK7) groups at 24 h after SAH. K-L , FJC staining in the cortex with quantification. Scale bar: 50 μm. ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: SIRT2 inhibitor AK7 (MCE, USA) was dissolved in dimethyl sulfoxide (DMSO).

Techniques: Expressing, Western Blot, Staining, Fluorescence

Journal: Journal of Neuroinflammation

Article Title: SIRT2 Inhibition promotes microglia LC3-associated phagocytosis via NRF2/CD36 after the experimental subarachnoid hemorrhage

doi: 10.1186/s12974-025-03623-z

Figure Lengend Snippet: SIRT2 inhibition promotes microglia phagocytosis. A-B , Western blot and quantitative analysis of SIRT2 expressions in microglia (Con, OxyHb, DMSO and AK7) groups. C-D ,phagocytic index level and analysis by pHrodo. E-F ,Immunofluorescence staining and quantitative analysis of LC3 and Phrodo in microglia. Scale bar: 10 μm. G-K , Western blot and quantitative analysis of Rubicon, NOX2, Beclin1 and LC3 A/B expressions. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: SIRT2 inhibitor AK7 (MCE, USA) was dissolved in dimethyl sulfoxide (DMSO).

Techniques: Inhibition, Western Blot, Immunofluorescence, Staining

Journal: Journal of Neuroinflammation

Article Title: SIRT2 Inhibition promotes microglia LC3-associated phagocytosis via NRF2/CD36 after the experimental subarachnoid hemorrhage

doi: 10.1186/s12974-025-03623-z

Figure Lengend Snippet: SIRT2 deacetylates NRF2 and promotes its nuclear translocation in microglia. A-C , Heatmap analyses and q-PCR detects different NRF2 target genes in microglia (SIRT2 and SIRT2 KD) groups. D , Molecular docking shown the obvious interaction force between SIRT2 and NRF2. E-G , Co-immunoprecipitation and quantitative analysis of acetylated NRF2 in SIRT2 WT and KD microglia. H , Western blot and quantitative analysis of SIRT2 in microglia (Con, OxyHb, DMSO and AK7) group with Cyto and nucleus. I-K , The co-localization of SIRT2 and nucleus with laser confocal and analysis in Con group and OxyHb group of microglia. Scale bar: 10 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n.s., no significant

Article Snippet: SIRT2 inhibitor AK7 (MCE, USA) was dissolved in dimethyl sulfoxide (DMSO).

Techniques: Translocation Assay, Immunoprecipitation, Western Blot

Journal: Journal of Neuroinflammation

Article Title: SIRT2 Inhibition promotes microglia LC3-associated phagocytosis via NRF2/CD36 after the experimental subarachnoid hemorrhage

doi: 10.1186/s12974-025-03623-z

Figure Lengend Snippet: SIRT2 through NRF2/CD36 regulates microglial phagocytosis. A , JASPAR predict NRF2 bind to the promoter region of CD36. B-C , phagocytic index level and analysis by pHrodo in microglia (Con, OxyHb, DMSO, AK7 and AK7+ML385) groups. D-E , Western blot and quantitative analysis of CD36 expression. F-H , ELISA quantitative analysis of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) level in different groups. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: SIRT2 inhibitor AK7 (MCE, USA) was dissolved in dimethyl sulfoxide (DMSO).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Neuroinflammation

Article Title: SIRT2 Inhibition promotes microglia LC3-associated phagocytosis via NRF2/CD36 after the experimental subarachnoid hemorrhage

doi: 10.1186/s12974-025-03623-z

Figure Lengend Snippet: SIRT2 via LAP inhibits phagocytosis in microglia. A-F ,Western blot and quantitative analysis of Rubicon, ULK1, CD36, NOX2 and LC3 A/B expressions. G-H , The co-localization of LC3 and pHrodo with laser confocal and analysis indifferent groups of microglia. Scale bar: 10 μm. I-J , Representative TEM images of LAPosomes in microglia. (The black triangle refers to the single membrane structure characteristic of LAPosomes and star refers to the Rubicon particles). Scale bar: 3 μm (upper panels), 1 μm (lower panels). * P < 0.05 vs. Hb, # P < 0.05 vs. Hb+AK7, & P < 0.05 vs. Hb+shRNA, $ P < 0.05 vs. Hb+AK7+shRNA, ☆ P < 0.05 vs. Hb+AK7+ML385, *** P < 0.001, **** P < 0.0001

Article Snippet: SIRT2 inhibitor AK7 (MCE, USA) was dissolved in dimethyl sulfoxide (DMSO).

Techniques: Western Blot, Membrane, shRNA

Journal: Journal of Neuroinflammation

Article Title: SIRT2 Inhibition promotes microglia LC3-associated phagocytosis via NRF2/CD36 after the experimental subarachnoid hemorrhage

doi: 10.1186/s12974-025-03623-z

Figure Lengend Snippet: Inhibition of SIRT2 improves long-term neurological function after SAH. A-B , The falling latency of rotarod test (5RPM and 10 RPM) at 1, 2, and 3 weeks after SAH in rats. C-E ,Escape latency and swimming distance of Morris water maze test on days 23 to 27 after SAH. F-G , Quantification of probe quadrant duration and swimming velocities. H-I , Representative images and neuronal quantifications of Nissl staining in CA1 hippocampal region. Scale bar: 200 μm (upper panels), 50 μm (lower panels). * P < 0.05, ** P< 0.01, *** P < 0.001, **** P < 0.0001, n.s., no significant

Article Snippet: SIRT2 inhibitor AK7 (MCE, USA) was dissolved in dimethyl sulfoxide (DMSO).

Techniques: Inhibition, Staining

Journal: Journal of Neuroinflammation

Article Title: SIRT2 Inhibition promotes microglia LC3-associated phagocytosis via NRF2/CD36 after the experimental subarachnoid hemorrhage

doi: 10.1186/s12974-025-03623-z

Figure Lengend Snippet: Schematic diagram of our study. SIRT2 modified NRF2 by deacetylation and inhibited its entry into the nucleus, affecting the transcription of downstream CD36, thereby reducing LAP in microglia

Article Snippet: SIRT2 inhibitor AK7 (MCE, USA) was dissolved in dimethyl sulfoxide (DMSO).

Techniques: Modification