Journal: Kidney Diseases

Article Title: Localization of Cell Receptor-Related Genes of SARS-CoV-2 in the Kidney through Single-Cell Transcriptome Analysis

doi: 10.1159/000508162

Figure Lengend Snippet: Expression of genes associated with SARS-CoV-2 entry in single-cell transcriptome sequencing database. a UMAP visualization displaying major cell clusters (22,268 single cells). b Dot plot of LRP2 and CUBN (marker genes of PT cells), ACE2, TMPRSS2, and SLC6A19; dot size represents the fraction of cells expressing a cell type, and color intensity binned count-based expression level amongst expressing cells. c UMAP projection, points colored by detection of ACE2 (left), TMPRSS2 (middle), and SLC6A19 (right). Blue, RNA positive; gray, RNA negative. d Volcano plot displaying differential expression of genes between ACE2+ and ACE2– PT cells. Red, up-regulated genes; blue, down-regulated genes. e Bar plot presenting significantly enriched GO terms obtained from GO enrichment analysis performed with differential expression of genes between ACE2+ and ACE2– PT cells.

Article Snippet: In the latest human kidney single-cell transcriptome database (scRNA-seq) from the Broad Institute and the University of Michigan [ ], we found significant expression of ACE2 in PT cells, glomerular parietal epithelial cells, and descending thin limb cells (Fig. ; online suppl.

Techniques: Expressing, Sequencing, Marker, Quantitative Proteomics

Journal: Kidney Diseases

Article Title: Localization of Cell Receptor-Related Genes of SARS-CoV-2 in the Kidney through Single-Cell Transcriptome Analysis

doi: 10.1159/000508162

Figure Lengend Snippet: Expression of genes associated with SARS-CoV-2 entry in single-nuclear transcriptome. a Dot plot of LRP2 and CUBN (marker genes of PT cells), ACE2, TMPRSS2, and SLC6A19; dot size represents the fraction of cells expressing each cell type, and color intensity binned count-based expression level amongst expressing cells. b Bar plot presenting significantly enriched GO terms obtained from GO enrichment analysis performed with differential expression of genes between ACE2+ and ACE2– PT cells. c Violin plot showing ACE2, SLC6A19, and TMPRSS2 expression distribution among different cell clusters.

Article Snippet: In the latest human kidney single-cell transcriptome database (scRNA-seq) from the Broad Institute and the University of Michigan [ ], we found significant expression of ACE2 in PT cells, glomerular parietal epithelial cells, and descending thin limb cells (Fig. ; online suppl.

Techniques: Expressing, Marker, Quantitative Proteomics

Journal: Kidney Diseases

Article Title: Localization of Cell Receptor-Related Genes of SARS-CoV-2 in the Kidney through Single-Cell Transcriptome Analysis

doi: 10.1159/000508162

Figure Lengend Snippet: Expression of genes associated with SARS-CoV-2 entry in a single-cell transcriptome sequencing database of Asians. a Dot plot of LRP2 and CUBN (marker genes of PT cells), ACE2, TMPRSS2, and SLC6A19; dot size represents the fraction of cells expressing each cell type, and color intensity binned count-based expression level amongst expressing cells. b Bar plot presenting significantly enriched GO terms obtained from GO enrichment analysis performed with differential expression of genes between ACE2+ and ACE2– PT cells.

Article Snippet: In the latest human kidney single-cell transcriptome database (scRNA-seq) from the Broad Institute and the University of Michigan [ ], we found significant expression of ACE2 in PT cells, glomerular parietal epithelial cells, and descending thin limb cells (Fig. ; online suppl.

Techniques: Expressing, Sequencing, Marker, Quantitative Proteomics