shp2 oe (MedChemExpress)
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MedChemExpress
shp2 oe
Shp2 Oe, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shp2+oe/SHP-2%2C+Human/pmc12905148-45-168-192
Average 94 stars, based on 3 article reviews
Shp2 Oe, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shp2+oe/SHP-2%2C+Human/pmc12905148-45-168-192
Average 94 stars, based on 3 article reviews
shp2 oe - by Bioz Stars,
2026-09
94/100 stars
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1) Product Images from "SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling"
Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling
Journal: Scientific Reports
doi: 10.1038/s41598-026-35493-7
Figure Legend Snippet: SHP2 overexpression promotes osteosarcoma proliferation by regulating the STAT3/TET3/HOXB2 and c-Myc/NANOG/NUSAP1 signaling pathways. ( A ) Western blot analysis of protein expression bands in 143B and MG63 cells, with statistical graphs showing relative protein expression levels. Groups: NC, SHP2-OE, SHP2-OE + Stattic, SHP2-OE + Stattic + TET3-OE, and SHP2-OE + Stattic + TET3-OE + HOXB2-KD. ( B ) Colony formation assay results assessing the proliferative capacity of 143B and MG63 cells, with statistical analysis graphs of colony numbers. ( C ) CCK-8 assay results showing the proliferative capacity of 143B and MG63 cells, with statistical analysis of proliferating cell counts. Data are presented as mean ± standard deviation. N = 3; P < 0.05 was considered statistically significant; ns P > 0.05, * P < 0.05, ** P < 0.01.
Techniques Used: Over Expression, Protein-Protein interactions, Western Blot, Expressing, Colony Assay, CCK-8 Assay, Standard Deviation
Figure Legend Snippet: SHP2-OE promotes osteosarcoma progression by upregulating STAT3, TET3, and HOXB2 proteins. ( A ) Experimental results of nude mouse subcutaneous tumor transplantation, including statistical analysis charts for tumor weight and volume. ( B ) Western blot analysis of protein expression bands in tumor tissues, with statistical comparison charts for relative protein expression levels. Mouse groups: NC group, SHP2-OE group, SHP2-OE + TET3-KD group, SHP2-OE + TET3-KD + HOXB2-OE group. ( C ) SHP2 promotes malignant progression of osteosarcoma through the STAT3/TET3/HOXB2 signaling pathway. Data are presented as mean ± SD. N = 6, P < 0.05 indicates statistically significant difference, ns P > 0.05, * P < 0.05, ** P < 0.01.
Techniques Used: Transplantation Assay, Western Blot, Expressing, Comparison
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Saline:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; SDS Page:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; Lysis:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; Protein Concentration:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; Polyacrylamide Gel Electrophoresis:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; Electrophoresis:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; Western Blot:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; Blocking Assay:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; Membrane:Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling Article Snippet: The human osteosarcoma cell line 143B and the human osteosarcoma cell line MG63 were purchased from Hunan Fenghui Biotechnology Co., Ltd. .. Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; |