Journal: Scientific Reports

Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling

doi: 10.1038/s41598-026-35493-7

Figure Lengend Snippet: SHP2 overexpression promotes osteosarcoma proliferation by regulating the STAT3/TET3/HOXB2 and c-Myc/NANOG/NUSAP1 signaling pathways. ( A ) Western blot analysis of protein expression bands in 143B and MG63 cells, with statistical graphs showing relative protein expression levels. Groups: NC, SHP2-OE, SHP2-OE + Stattic, SHP2-OE + Stattic + TET3-OE, and SHP2-OE + Stattic + TET3-OE + HOXB2-KD. ( B ) Colony formation assay results assessing the proliferative capacity of 143B and MG63 cells, with statistical analysis graphs of colony numbers. ( C ) CCK-8 assay results showing the proliferative capacity of 143B and MG63 cells, with statistical analysis of proliferating cell counts. Data are presented as mean ± standard deviation. N = 3; P < 0.05 was considered statistically significant; ns P > 0.05, * P < 0.05, ** P < 0.01.

Article Snippet: Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; SHP2-OE, TET3-KD, SHP2-KD, and HOXB2-OE lentiviruses were purchased from Shanghai Jiman Biological Company; STAT3 agonist Colivelin Stattic and TET inhibitor TETi76 were purchased from MCE.

Techniques: Over Expression, Protein-Protein interactions, Western Blot, Expressing, Colony Assay, CCK-8 Assay, Standard Deviation

Journal: Scientific Reports

Article Title: SHP2 promotes osteosarcoma via regulating STAT3/TET3/HOXB2 signaling

doi: 10.1038/s41598-026-35493-7

Figure Lengend Snippet: SHP2-OE promotes osteosarcoma progression by upregulating STAT3, TET3, and HOXB2 proteins. ( A ) Experimental results of nude mouse subcutaneous tumor transplantation, including statistical analysis charts for tumor weight and volume. ( B ) Western blot analysis of protein expression bands in tumor tissues, with statistical comparison charts for relative protein expression levels. Mouse groups: NC group, SHP2-OE group, SHP2-OE + TET3-KD group, SHP2-OE + TET3-KD + HOXB2-OE group. ( C ) SHP2 promotes malignant progression of osteosarcoma through the STAT3/TET3/HOXB2 signaling pathway. Data are presented as mean ± SD. N = 6, P < 0.05 indicates statistically significant difference, ns P > 0.05, * P < 0.05, ** P < 0.01.

Article Snippet: Fetal bovine serum (FBS) was purchased from Global Kang Qinhuangdao Biotechnology Co., Ltd.; DMEM basal medium, penicillin-streptomycin solution (double antibiotics), phosphate-buffered saline (PBS), and 0.05% trypsin were obtained from Wuhan Procell Life Science & Technology Co., Ltd.; Matrigel was sourced from Corning, USA; Transwell chambers, dimethyl sulfoxide (DMSO), and SDS-PAGE protein loading buffer were acquired from Shanghai Beyotime Biotechnology Co., Ltd.; High-efficiency RIPA lysis buffer and protease phosphatase inhibitors were purchased from Beijing Solarbio Science & Technology Co., Ltd.; The BCA protein concentration assay kit was obtained from GLPBIO, USA; The one-step PAGE quick preparation kit, SDS-PAGE rapid electrophoresis buffer, rapid transfer buffer, high-efficiency Western blocking buffer, and universal antibody diluent were procured from Shanghai Jifu Life Science Co., Ltd.; PVDF membrane was sourced from Merck, Germany; p-STAT3 antibody, nuclear TET3 antibody, nuclear HOXB2 antibody, c-Myc antibody, NANOG antibody, and NUSAP1 antibody were purchased from Jiangsu Qinke Biological Research Center Co., Ltd.; Goat anti-rabbit IgG (H + L) HRP antibody was obtained from Wuhan Sanying Biotechnology Co., Ltd.; SHP2-OE, TET3-KD, SHP2-KD, and HOXB2-OE lentiviruses were purchased from Shanghai Jiman Biological Company; STAT3 agonist Colivelin Stattic and TET inhibitor TETi76 were purchased from MCE.

Techniques: Transplantation Assay, Western Blot, Expressing, Comparison