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anti sesn2 (Proteintech)

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Proteintech anti sesn2
Anti Sesn2, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sesn2/Sestrin+2+Polyclonal+antibody/10__3390_slash_cells15060505-75-110-112
Average 95 stars, based on 155 article reviews

anti sesn2 - by Bioz Stars, 2026-09

95/100 stars

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RNA Binding Assay:

Article Title: Rhoifolin prevents herpes simplex virus encephalitis by modulating SESN2 to regulate the Keap1/Nrf2 and AMPK/Nox pathways to inhibit neuro-oxidative damage in mice.
Article Snippet: Background: Herpes simplex encephalitis (HSE), predominantly caused by herpes simplex virus type 1 (HSV-1), is a life-threatening central nervous system disease and the leading cause of sporadic encephalitis globally.. Currently, the treatment for HSE mainly relies on nucleoside drugs such as acyclovir (ACV), but the mortality rate remains 10 %-25 % after treatment, with about half of the survivors suffering from neurological sequelae.. Rhoifolin (ROF) is a chromatographic standard component specified in the pharmacopoeia of the traditional Chinese medicine Turpinia leaf.

Incubation:

Article Title: Sestrin2 Exerts a Novel Protective Effect Against LPS ‐Induced Ferroptosis via the Nrf2– SLC7A11 – GPX4 Signaling Axis
Article Snippet: Following the transfer of separated proteins onto PVDF membranes (Millipore), the membranes were incubated with TBST containing either 5% skim milk or 1% BSA for blocking. .. The membranes were incubated with primary antibodies including GADPH (Proteintech Cat# CL488‐60004, RRID:AB_2919223), p62 (Abcam Cat# ab109012, RRID:AB_2810880), SLC7A11 (Proteintech Cat# 26864‐1‐AP, RRID:AB_2880661), FTH1 (4393T; Cell Signaling Technology, RRID:AB_11217441), SOD1 (Affinity Biosciences Cat# AF5198, RRID:AB_2837684), SOD2 (Proteintech Cat# 24127‐1‐AP, RRID:AB_2879437), GPX4 (Proteintech Cat# 30388‐1‐AP, RRID:AB_3086304), 4‐HNE (Bioss Cat# bs‐6313R, RRID:AB_2827741), NRF2 (Proteintech Cat# 80593‐1‐RR, RRID:AB_2918904), P‐NRF2 (Bioss Cat# BSM‐52179R, RRID:AB_3492114), and SESN2 (Proteintech Cat# 66297‐1‐Ig, RRID:AB_2881680). ..

Article Title: SS-31 improves post-cardiac arrest brain injury by inhibiting microglial ferroptosis and polarization
Article Snippet: .. Protein samples were separated by SDS-PAGE and transferred to PVDF membranes, which were blocked with 5 % skim milk and incubated overnight at 4 °C with primary antibodies (NRF2, 1:1000, 16396-1-AP; COX2, 1:1000, 12375-1-AP; SLC7A11, 1:1000, 26864-1-AP; HO-1, 1:1000, 10701-1-AP; GPX4, 1:1000, 67763-1-Ig; Sesn2, 1:1000, 10795-1-AP; CD206, 1:1000, 18704-1-AP; INOS, 1:1000, 18985-1-AP; β-actin, 1:1000, 66009-1-Ig; all from Proteintech, China). .. The membranes were then incubated with goat anti-mouse or goat anti-rabbit secondary antibodies (Proteintech, China) for 2 h at room temperature.

Article Title: Lactoferrin-cyanidin-3-glucoside nanoparticles alleviate inflammation and oxidative stress via Sesn2/Nrf2 activation in mastitis
Article Snippet: .. Following this, sections were blocked using goat serum to minimize non-specific binding and incubated overnight at 4 °C with a rabbit-derived primary antibody specific for SESN2 (Proteintech, 66297-1-Ig), Nrf2 (Proteintech, 16396-1-AP), and TNF-α (Abmart, PY19810) at a 1:300 dilution. ..

Article Title: Sestrin 2 Regulates Milk Protein Synthesis through a LAT1/mTOR Pathway.
Article Snippet: Sestrin 2, coded by the SESN2 gene, as a leucine sensor, can recognize and bind intracellular free leucine, regulate the mammalian target of rapamycin (mTOR) signaling pathway, and participate in protein synthesis, but its molecular mechanism in the mammary gland is still unclear.. The aim of this study is to investigate the molecular mechanism of SESN2 on milk protein synthesis in bovine mammary epithelial cells (MAC-T) and further confirm its lactation-regulating function in the mouse mammary gland.. In this study, it was found that, after SESN2 knockdown (KD), the phosphorylation levels of mTOR protein and downstream signaling molecule S6K were increased and the expression of αand β-casein was increased in MAC-T cells.

Article Title: SESN2 maintains cartilage homeostasis by SREBP1-mediated lipid metabolism during osteoarthritis progression
Article Snippet: .. Slides were incubated overnight at 4°C with primary antibodies: SESN2 (Proteintech, #66297-1-Ig, 1:200), FASN (Proteintech, #66591-1-Ig, 1:100), SCD1 (ABclonal, #A16429, 1:100), SREBP1 (Proteintech, #14088-1-AP, 1:200), PGC-1α (Abcam, #ab54481, 1:100). ..

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other:

Article Title: Arsenic regulates ALKBH1 abundance and substrate specificity to promote translation and tumorigenicity
Article Snippet: SESN2 , Proteintech , Cat# 10795–1-AP; RRID:AB_2185480.

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Validation of primary antibodies against SESN2, SESN3, and SOD1 using ICW assay. (A) AN3CA and KLE cells were probed with anti-SESN2 antibody (dilutions 1:1200–1:150). (B) AN3CA and KLE cells were probed with anti-SESN3 antibody (dilutions 1:1000–1:100). (C) AN3CA and KLE cells were probed with anti-SOD1 antibody (dilutions 1:1000–1:100). Secondary antibodies (1:800, LI-COR) were applied, with CellTag™ 520 (1:500, LI-COR) as a loading control for signal normalization. Data are shown as mean ± SD of three technical replicate wells from a single antibody-optimization experiment. Statistical significance was assessed by two-way ANOVA (factors: cell line and antibody dilution), followed by Sidak’s multiple-comparisons test comparing AN3CA vs. KLE within each dilution. **p < 0.005.

Journal: Frontiers in Physiology

Article Title: Oxidative stress-mediated responses in endometrial cancer cells: contrasting effects of doxorubicin and menadione

doi: 10.3389/fphys.2026.1733194

Figure Lengend Snippet: Validation of primary antibodies against SESN2, SESN3, and SOD1 using ICW assay. (A) AN3CA and KLE cells were probed with anti-SESN2 antibody (dilutions 1:1200–1:150). (B) AN3CA and KLE cells were probed with anti-SESN3 antibody (dilutions 1:1000–1:100). (C) AN3CA and KLE cells were probed with anti-SOD1 antibody (dilutions 1:1000–1:100). Secondary antibodies (1:800, LI-COR) were applied, with CellTag™ 520 (1:500, LI-COR) as a loading control for signal normalization. Data are shown as mean ± SD of three technical replicate wells from a single antibody-optimization experiment. Statistical significance was assessed by two-way ANOVA (factors: cell line and antibody dilution), followed by Sidak’s multiple-comparisons test comparing AN3CA vs. KLE within each dilution. **p < 0.005.

Article Snippet: PrimePCRTM Probe Assay was used for SOD1 (ID: qHsaCIP0026883, Bio-Rad), while TaqMan® Gene Expression Assays were used for SESN2 (ID: Hs00230241_m1), SESN3 (ID: Hs00914870_m1), and UBC (ID: Hs00824723_m1) (Thermo Fisher Scientific, Waltham, MA, United States).

Techniques: Biomarker Discovery, Control

Differential effects of DOX and menadione on SESN2 expression in AN3CA and KLE cells assessed by ICW assay. (A) In AN3CA cells, menadione (1–25 µM) induced a modest upward trend in normalized SESN2 levels. (B) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). (C) In KLE cells, menadione increased normalized SESN2 levels at 1–5 μM, followed by a marked decrease at 25 µM. (D) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). (E) In AN3CA cells treated with DOX (0.125–4 µM), SESN2 exhibited a biphasic response, peaking at 0.125 µM and declining sharply from 1 µM onward. (F) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). (G) In KLE cells, DOX induced modest SESN2 upregulation at 0.125–0.25 µM, followed by progressive downregulation with the lowest levels at 4 µM. (H) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). Normalized protein levels were calculated as SESN2/CellTag™ 520 ratios (normalized signal). Untreated control (medium-only) wells were included in each experiment and served as the reference group for comparisons. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment condition to the untreated control. For DOX, the figure shows a representative ICW run (n = 6 technical replicate wells per condition), and the experiment was repeated independently once with consistent results (n = 3). For menadione, data are from one ICW experiment (n = 6 technical replicate wells per condition). *p < 0.05; **p < 0.005; ***p < 0.0005.

Journal: Frontiers in Physiology

Article Title: Oxidative stress-mediated responses in endometrial cancer cells: contrasting effects of doxorubicin and menadione

doi: 10.3389/fphys.2026.1733194

Figure Lengend Snippet: Differential effects of DOX and menadione on SESN2 expression in AN3CA and KLE cells assessed by ICW assay. (A) In AN3CA cells, menadione (1–25 µM) induced a modest upward trend in normalized SESN2 levels. (B) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). (C) In KLE cells, menadione increased normalized SESN2 levels at 1–5 μM, followed by a marked decrease at 25 µM. (D) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). (E) In AN3CA cells treated with DOX (0.125–4 µM), SESN2 exhibited a biphasic response, peaking at 0.125 µM and declining sharply from 1 µM onward. (F) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). (G) In KLE cells, DOX induced modest SESN2 upregulation at 0.125–0.25 µM, followed by progressive downregulation with the lowest levels at 4 µM. (H) Raw fluorescence signals corresponding to SESN2 (700 nm) and CellTag™ 520 (520 nm). Normalized protein levels were calculated as SESN2/CellTag™ 520 ratios (normalized signal). Untreated control (medium-only) wells were included in each experiment and served as the reference group for comparisons. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment condition to the untreated control. For DOX, the figure shows a representative ICW run (n = 6 technical replicate wells per condition), and the experiment was repeated independently once with consistent results (n = 3). For menadione, data are from one ICW experiment (n = 6 technical replicate wells per condition). *p < 0.05; **p < 0.005; ***p < 0.0005.

Techniques: Expressing, Fluorescence, Control

SESN2, SESN3, and SOD1 gene expression profiles in AN3CA and KLE cells analyzed by qRT-PCR. (A) SESN2 mRNA was significantly upregulated in both cell lines following 4 µM DOX treatment. Menadione induced robust SESN2 upregulation at 25 µM in KLE cells. (B) SESN3 mRNA was strongly upregulated by DOX in both lines, whereas menadione downregulated SESN3 in both cell lines. (C) SOD1 mRNA showed significant upregulation in KLE cells under 4 µM DOX, while AN3CA cells exhibited no significant changes. Under menadione, AN3CA cells displayed significant downregulation of SOD1 at 10 µM and 25 μM, while KLE cells showed downregulation at 25 µM. RT–qPCR data were normalized to UBC as the endogenous reference gene and are presented relative to the untreated control (medium-only). Each condition was analyzed in three technical replicate wells. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment condition to the untreated control. Key findings were confirmed in an independent repeat experiment. *p < 0.05; **p < 0.005; ***p < 0.0005.

Journal: Frontiers in Physiology

Article Title: Oxidative stress-mediated responses in endometrial cancer cells: contrasting effects of doxorubicin and menadione

doi: 10.3389/fphys.2026.1733194

Figure Lengend Snippet: SESN2, SESN3, and SOD1 gene expression profiles in AN3CA and KLE cells analyzed by qRT-PCR. (A) SESN2 mRNA was significantly upregulated in both cell lines following 4 µM DOX treatment. Menadione induced robust SESN2 upregulation at 25 µM in KLE cells. (B) SESN3 mRNA was strongly upregulated by DOX in both lines, whereas menadione downregulated SESN3 in both cell lines. (C) SOD1 mRNA showed significant upregulation in KLE cells under 4 µM DOX, while AN3CA cells exhibited no significant changes. Under menadione, AN3CA cells displayed significant downregulation of SOD1 at 10 µM and 25 μM, while KLE cells showed downregulation at 25 µM. RT–qPCR data were normalized to UBC as the endogenous reference gene and are presented relative to the untreated control (medium-only). Each condition was analyzed in three technical replicate wells. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment condition to the untreated control. Key findings were confirmed in an independent repeat experiment. *p < 0.05; **p < 0.005; ***p < 0.0005.

Techniques: Gene Expression, Quantitative RT-PCR, Control

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