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Cell Signaling Technology Inc serca2a
Serca2a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serca2a/ATP2A2%2FSERCA2+Rabbit+mAb/pm41707812-100-23-24
Average 94 stars, based on 89 article reviews

serca2a - by Bioz Stars, 2026-10

94/100 stars

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Article Title: Cellular and electrophysiological characterization of triadin knockout syndrome using induced pluripotent stem cell-derived cardiomyocytes
Article Snippet: .. After fixing, cells were washed 3 times with PBS, permeabilized/blocked with PBS containing 0.1% Triton X-100 (PBST)/5% goat serum for 1 hour at RT, then incubated overnight in primary antibody solution made of PBST/5% goat serum containing a 1:200 dilution of primary antibody against cTnT (rabbit, Abcam, ab45932), α-Actinin (mouse, Sigma, A7811), MLC2V (mouse, BD Biosciences, 565497), RyR2 (mouse, ThermoFisher, MA3-916), Casq2 (rabbit, Invitrogen, PA5-621706), CaV1.2 (rabbit, Alomone Labs, ACC-003), or SERCA2a (rabbit, Cell Signaling, 4388S). .. PCR products with BanI digestion pattern were sequenced by GenHunter (Brookline, MA), and FinchTV was used to analyze sequences.

Article Title: Cellular and electrophysiological characterization of triadin knockout syndrome using induced pluripotent stem cell-derived cardiomyocytes
Article Snippet: .. After fixing, cells were washed 3 times with PBS, permeabilized/blocked with PBS containing 0.1% Triton X-100 (PBST)/5% goat serum for 1 hour at RT, then incubated overnight in primary antibody solution made of PBST/5% goat serum containing a 1:200 dilution of primary antibody against cTnT (rabbit, Abcam, ab45932), α-Actinin (mouse, Sigma, A7811), MLC2V (mouse, BD Biosciences, 565497), RyR2 (mouse, ThermoFisher, MA3-916), Casq2 (rabbit, Invitrogen, PA5-621706), CaV1.2 (rabbit, Alomone Labs, ACC-003), or SERCA2a (rabbit, Cell Signaling, 4388S). .. The following day, cells were washed 3 times with PBST at RT before being incubated in PBST/5% goat serum with a 1:250 dilution of Alexa Fluor 488 goat-anti-rabbit (Thermo Fisher Scientific, A11008) and Alexa Fluor 594 goat-anti-mouse (Thermo Fisher Scientific, A11005) secondary antibodies at RT for 1 hour.

Article Title: SARS-CoV-2 spike protein-mediated cardiomyocyte fusion may contribute to increased arrhythmic risk in COVID-19.
Article Snippet: .. After fixing, cells were washed 3 times with PBS, permeabilized/blocked with PBS containing 0.1% Triton X-100 (PBST)/5% goat serum for 1 hour at RT, then incubated overnight in primary antibody solution made of PBST/5% goat serum containing primary antibody against α- PLOS ONE | https://doi.org/10.1371/journal.pone.0282151 March 8, 2023 3 / 16 Actinin (1:500 dilution, mouse, Sigma, A7811), calreticulin (1:200 dilution, rabbit, Abcam, ab2907) or SERCA2a (rabbit, Cell Signaling, 4388S). .. The following day, cells were washed 3 times with PBST at RT before being incubated in PBST/5% goat serum with a 1:250 dilution of Alexa Fluor 488 goat-anti-mouse (Thermo Fisher Scientific, A11001) and Alexa Fluor 594 goat-anti-rabbit (Thermo Fisher Scientific, A11037) secondary antibodies at RT for 1 hour.

Article Title: Cellular and electrophysiological characterization of triadin knockout syndrome using induced pluripotent stem cell-derived cardiomyocytes
Article Snippet: Protein was transferred onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA). .. The membrane was incubated with primary antibody against human Trisk 32 (1:20,000 dilution, rabbit, described by Roux-Buisson et al.5), β-Actin (1:2,000 dilution, mouse, Santa Cruz Biotechnology, sc-47778), RyR2 (1:2,000 dilution, mouse, ThermoFisher, MA3-916), Casq2 (1:1,000 dilution, rabbit, Invitrogen, PA5-621706), CaV1.2 (1,000 dilution, mouse, Invitrogen, MA5-27717), SERCA2a (1:1,000 dilution, rabbit, Cell Signaling, 4388S), or α-Actinin (1:2,000 dilution, mouse, Sigma, A7811) overnight at 4oC. .. The electroporated cells were plated on Matrigel (Corning, Corning, NY)-coated plates in mTeSR1 media (STEMCELL Technologies, Cambridge, MA).

Article Title: Cellular and electrophysiological characterization of triadin knockout syndrome using induced pluripotent stem cell-derived cardiomyocytes
Article Snippet: Protein was transferred onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA). .. The membrane was incubated with primary antibody against human Trisk 32 (1:20,000 dilution, rabbit, described by Roux-Buisson et al.5), β-Actin (1:2,000 dilution, mouse, Santa Cruz Biotechnology, sc-47778), RyR2 (1:2,000 dilution, mouse, ThermoFisher, MA3-916), Casq2 (1:1,000 dilution, rabbit, Invitrogen, PA5-621706), CaV1.2 (1,000 dilution, mouse, Invitrogen, MA5-27717), SERCA2a (1:1,000 dilution, rabbit, Cell Signaling, 4388S), or α-Actinin (1:2,000 dilution, mouse, Sigma, A7811) overnight at 4oC. .. Membranes were then incubated in secondary antibody (Goat anti-rabbit, 1:2,500, Invitrogen, Carlsbad, CA, 656120; Goat anti-mouse, 1:2,000 R&D Systems, Minneapolis, MN, HAF007) for 1 hour at RT.

Membrane:

Article Title: Cellular and electrophysiological characterization of triadin knockout syndrome using induced pluripotent stem cell-derived cardiomyocytes
Article Snippet: Protein was transferred onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA). .. The membrane was incubated with primary antibody against human Trisk 32 (1:20,000 dilution, rabbit, described by Roux-Buisson et al.5), β-Actin (1:2,000 dilution, mouse, Santa Cruz Biotechnology, sc-47778), RyR2 (1:2,000 dilution, mouse, ThermoFisher, MA3-916), Casq2 (1:1,000 dilution, rabbit, Invitrogen, PA5-621706), CaV1.2 (1,000 dilution, mouse, Invitrogen, MA5-27717), SERCA2a (1:1,000 dilution, rabbit, Cell Signaling, 4388S), or α-Actinin (1:2,000 dilution, mouse, Sigma, A7811) overnight at 4oC. .. The electroporated cells were plated on Matrigel (Corning, Corning, NY)-coated plates in mTeSR1 media (STEMCELL Technologies, Cambridge, MA).

Article Title: Cellular and electrophysiological characterization of triadin knockout syndrome using induced pluripotent stem cell-derived cardiomyocytes
Article Snippet: Protein was transferred onto a PVDF membrane using a Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA). .. The membrane was incubated with primary antibody against human Trisk 32 (1:20,000 dilution, rabbit, described by Roux-Buisson et al.5), β-Actin (1:2,000 dilution, mouse, Santa Cruz Biotechnology, sc-47778), RyR2 (1:2,000 dilution, mouse, ThermoFisher, MA3-916), Casq2 (1:1,000 dilution, rabbit, Invitrogen, PA5-621706), CaV1.2 (1,000 dilution, mouse, Invitrogen, MA5-27717), SERCA2a (1:1,000 dilution, rabbit, Cell Signaling, 4388S), or α-Actinin (1:2,000 dilution, mouse, Sigma, A7811) overnight at 4oC. .. Membranes were then incubated in secondary antibody (Goat anti-rabbit, 1:2,500, Invitrogen, Carlsbad, CA, 656120; Goat anti-mouse, 1:2,000 R&D Systems, Minneapolis, MN, HAF007) for 1 hour at RT.

Image Search Results

FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and SERCA2a levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

Journal: Journal of Molecular Cell Biology

Article Title: Canonical Wnt signaling affects calcium homeostasis in serum-treated AC16 cells through MLN-mediated SERCA2a regulation

doi: 10.1093/jmcb/mjaf050

Figure Lengend Snippet: FBS-induced RyR2 ablation underlies impaired Wnt responsiveness in AC16 cells. ( A and B ) Cytosol and SR calcium levels of AC16 cells preincubated with H 2 O or CHX (1 μg/ml) for 15 min before treatment with Wnt modulators for 6 h. Error bars indicate SD ( n = 6). ( C and E ) Western blot analysis of RyR2 and SERCA2a levels in AC16 cells in the medium with or without FBS treated with SFRP2 (10 μg/ml) or LiCl (20 mM) for 6 h. Error bars indicate SD ( n = 3). ( D and F ) Relative RyR2 and SERCA2a mRNA levels in AC16 cells with or without FBS treated with SFRP2 or LiCl for 6 h. Error bars indicate SD ( n = 6). ( G ) Relative RyR2 mRNA level for ‘RyR2-reconstituted’ cells compared to AC16 cells without FBS. ( H and I ) Cytosol and SR calcium levels of AC16 cells in FBS medium (labeled ‘Without RyR2’) and ‘RyR2-reconstituted’ cells in FBS medium (labeled ‘With RyR2’). ( J and K ) Cytosol and SR calcium levels of ‘RyR2-reconstituted’ cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

Article Snippet: Monoclonal anti-SERCA2a antibody (ABclonal, A11692), monoclonal anti-RyR2 (Abcam, ab224803), polyclonal anti-MLN (Thermofisher, MYREGLN-101AP), monoclonal anti-tubulin antibody (Abcam, ab7291), and monoclonal anti-GAPDH (Abcam, ab8245) were used.

Techniques: Western Blot, Labeling

Wnt inhibition upregulates MLN expression to suppress SERCA2a activity. ( A ) Relative SERCA2a activity in AC16 cells in FBS medium. ( B ) Gene expression of known SERCA2a modulators in AC16 cells with or without FBS treated with different Wnt modulators (LiCl: 20 mM; SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) for 6 h. ( C ) Relative MLN mRNA level in AC16 cells with FBS treatment. ( D ) Western blot analysis of MLN in AC16 cells with FBS treatment. ( E and F ) Cytosol and SR calcium levels of MLN-knockdown cell lines in FBS medium. ( G and H ) Cytosol and SR calcium levels of MLN-kd2 AC16 cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

Journal: Journal of Molecular Cell Biology

Article Title: Canonical Wnt signaling affects calcium homeostasis in serum-treated AC16 cells through MLN-mediated SERCA2a regulation

doi: 10.1093/jmcb/mjaf050

Figure Lengend Snippet: Wnt inhibition upregulates MLN expression to suppress SERCA2a activity. ( A ) Relative SERCA2a activity in AC16 cells in FBS medium. ( B ) Gene expression of known SERCA2a modulators in AC16 cells with or without FBS treated with different Wnt modulators (LiCl: 20 mM; SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) for 6 h. ( C ) Relative MLN mRNA level in AC16 cells with FBS treatment. ( D ) Western blot analysis of MLN in AC16 cells with FBS treatment. ( E and F ) Cytosol and SR calcium levels of MLN-knockdown cell lines in FBS medium. ( G and H ) Cytosol and SR calcium levels of MLN-kd2 AC16 cells in FBS medium and AC16 cells without FBS. Error bars indicate SD ( n = 6).

Techniques: Inhibition, Expressing, Activity Assay, Gene Expression, Western Blot, Knockdown

Combined MLN knockdown and RyR2 overexpression restores canonical Wnt-mediated calcium regulation in FBS-treated AC16 cells. ( A ) Relative SERCA2a activity in FBS-treated AC16 cells and MLN-kd2 cells under various treatments. Cells were treated with the SERCA2a activator SAA (1 μM), the Wnt inhibitor SFRP2 (10 μg/ml), or the SERCA2a inhibitor Tg (100 nM) for 6 h. MLN knockdown significantly increased basal SERCA2a activity and abolished SFRP2-induced suppression. ( B ) Relative SERCA2a activity in MLN-kd2 cells treated with canonical Wnt inhibitors (SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) or the SERCA2a activator SAA (1 μM) for 6 h. ( C and D ) Cytosol and SR calcium levels in modified AC16 cells (‘New AC16 cells’) with combined MLN knockdown and RyR2 overexpression, compared to standard FBS-free AC16 cells and FBS-treated AC16 cells. Cells were treated with the Wnt activator LiCl (20 mM) or the Wnt inhibitor SFRP2 (10 μg/ml) for 6 h. Error bars indicate SD ( n = 6).

Journal: Journal of Molecular Cell Biology

Article Title: Canonical Wnt signaling affects calcium homeostasis in serum-treated AC16 cells through MLN-mediated SERCA2a regulation

doi: 10.1093/jmcb/mjaf050

Figure Lengend Snippet: Combined MLN knockdown and RyR2 overexpression restores canonical Wnt-mediated calcium regulation in FBS-treated AC16 cells. ( A ) Relative SERCA2a activity in FBS-treated AC16 cells and MLN-kd2 cells under various treatments. Cells were treated with the SERCA2a activator SAA (1 μM), the Wnt inhibitor SFRP2 (10 μg/ml), or the SERCA2a inhibitor Tg (100 nM) for 6 h. MLN knockdown significantly increased basal SERCA2a activity and abolished SFRP2-induced suppression. ( B ) Relative SERCA2a activity in MLN-kd2 cells treated with canonical Wnt inhibitors (SFRP2: 10 μg/ml; XAV939: 10 μM; LF3: 1 μM) or the SERCA2a activator SAA (1 μM) for 6 h. ( C and D ) Cytosol and SR calcium levels in modified AC16 cells (‘New AC16 cells’) with combined MLN knockdown and RyR2 overexpression, compared to standard FBS-free AC16 cells and FBS-treated AC16 cells. Cells were treated with the Wnt activator LiCl (20 mM) or the Wnt inhibitor SFRP2 (10 μg/ml) for 6 h. Error bars indicate SD ( n = 6).

Techniques: Knockdown, Over Expression, Activity Assay, Modification

(A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.

Journal: bioRxiv

Article Title: Dual effects of nicotinamide on aging-related arrhythmia: protective at low dose, proarrhythmic at high doses

doi: 10.1101/2025.09.22.677785

Figure Lengend Snippet: (A) Experimental scheme of Langendorff ex vivo mouse hearts perfused with 0 mM NAM (control) or 100 mM NAM-supplemented buffer. (B) Representative electrocardiogram (ECG) shows that acute perfusion with 100 mM NAM rapidly induced VF, contractile dysfunction, and eventually led to cardiac arrest (n=4 males, 2 females). (C) Representiative blots and (D) quantification of acetylated SERCA2a levels of left venticular tissue from control and 100 mM NAM-perfused ex vivo mouse hearts (n=3). Data are expressed as mean ± standard error of the mean. Two tailed unpaired Student t-test was used.

Article Snippet: The membrane was then blocked with Intercept Blocking Buffer (927-60001, LI-COR) for 1 h at RT, and incubated with primary antibodies against SERCA2a (1:1000 dilution, 4388, cell signaling), and Pan Acetylation (1:2000 dilution, 66289-1-Ig, Proteintech) overnight at 4°C.

Techniques: Ex Vivo, Control, Two Tailed Test

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