Journal: British journal of pharmacology

Article Title: CDN1163, an activator of sarco/endoplasmic reticulum Ca 2+ ATPase, up-regulates mitochondrial functions and protects against lipotoxicity in pancreatic β-cells.

doi: 10.1111/bph.16160

Figure Lengend Snippet: FIGURE 5 CDN1163-increased mitochondrial biogenesis and insulin secretion is dependent on SERCA2. Up-regulating responses in IP3 receptor and mitochondrial biogenesis by CDN1163 were further augmented by SERCA2a or 2b overexpression (n = 5) (a, b). After co- transfection of SERCA2 with growth hormone, glucose-stimulated growth hormone secretion was measured, reflecting insulin secretory functions (c). Knockdown effects of SERCA2 on IP3 receptor and mitochondrial biogenesis (n = 6) (d, e). Potentiating effects of CDN1163 on insulin secretion were abolished after suppression of SERCA2 in MIN6 cells (n = 6) (f). Data shown are individual values with means ± SEM. *P<0.05, significantly different as indicated; one-way (b, c) or two-way (f) ANOVA with Tukey's comparison tests.

Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense The human SERCA2a plasmid and human SERCA2b plasmid (Cat 75188) was from Addgene (Watertown,MA, USA); siATP2a2 (Cat. M040968-02-0050) was from Dharmacon (Lafayette, CO, USA).

Techniques: Over Expression, Cotransfection, Knockdown, Comparison

Journal: British journal of pharmacology

Article Title: CDN1163, an activator of sarco/endoplasmic reticulum Ca 2+ ATPase, up-regulates mitochondrial functions and protects against lipotoxicity in pancreatic β-cells.

doi: 10.1111/bph.16160

Figure Lengend Snippet: FIGURE 6 CDN1163 protects against palmitate-induced secretory defects and lipotoxicity. Transient transfection of SERCA2a or 2b restored glucose-stimulated hormone secretion (n = 6) (a). Defective insulin secretion in palmitate-treated mouse islets was also restored by CDN1163 (n = 6) (b). Palmitate incubation impaired glucose-stimulated Ca2+ oscillation (n = 5) (c), ER Ca2+ content (n = 5) (d) and mitochondrial membrane potential (n = 9) (e), all of which are protected by CDN1163 co-treatment. Immunostaining of mouse islets showed a reduction of insulin- secreting β-cells by palmitate, which was protected by CDN1163 (f). Apoptotic cell death induced by palmitate, as measured by TUNEL staining, was also prevented by CDN1163 (n = 5) (g). Data shown are means ± SEM (a, b, d, e, g) or SD (c). *P<0.05, significantly different as indicated; one way ANOVA.

Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense The human SERCA2a plasmid and human SERCA2b plasmid (Cat 75188) was from Addgene (Watertown,MA, USA); siATP2a2 (Cat. M040968-02-0050) was from Dharmacon (Lafayette, CO, USA).

Techniques: Transfection, Incubation, Membrane, Immunostaining, TUNEL Assay, Staining

Journal: Analytical Chemistry

Article Title: Effects of Physiologic Mechanical Stimulation on Embryonic Chick Cardiomyocytes Using a Microfluidic Cardiac Cell Culture Model

doi: 10.1021/ac503716z

Figure Lengend Snippet: Graphs of interested protein with ventricular proteins set as the control state. (A) SERCA2a protein synthesis, (B) α-MHC protein synthesis, (C) β-MHC protein synthesis, (D) TnT protein synthesis, and (E) α-actinin protein synthesis. N ≥ 3 and two tailed significance was set at * p < 0.05 and ** p < 0.005.

Article Snippet: After blocked with 5% nonfat milk, the membrane was probed with different primary antibodies including anti-SERCA2a (1:500) (LSBio, Seattle, WA), anti α-MHC (1:150) (Santa Cruz, Dallas, TX) and β-MHC (1:500) (Bioss, Woburn, MA), anti-Actinin (1:500) (Abcam, Cambridge, MA), anti TnT (1:1000) (Sigma-Aldrich, St. Louis, MO), and anti β-actin (1:1000) (Cell Signaling, Beverly, MA).

Techniques: Two Tailed Test