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smart seq mrna lp kit  (TaKaRa)


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    Structured Review

    TaKaRa smart seq mrna lp kit
    Smart Seq Mrna Lp Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/SMART-Seq+mRNA+LP/pm42826831-64-6-10
    Average 98 stars, based on 69 article reviews
    smart seq mrna lp kit - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    other:

    Article Title: Genome-wide cell-free DNA methylation profiling via TAPS for moyamoya disease diagnosis and postoperative collateral formation prediction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Plasma samples from patients with moyamoya disease (MMD) and atherosclerotic cerebrovascular disease (ACVD) Zhongnan Hospital of Wuhan University Nanjing Drum Tower Hospital, Guangdong Sanjiu Brain Hospital, and Hainan Second People’s Hospital N/A Middle cerebral artery (MCA) samples from patients with MMD Zhongnan Hospital of Wuhan University N/A Lambda DNA Thermo Fisher Cat. #SD0021 Chemicals, peptides, and recombinant proteins α-ketoglutarate Sigma Cat. #K3752 Adenosine triphosphate (ATP) Sigma Cat. #A6419 Dithiothreitol (DTT) Biofroxx Cat. #1111GR005 Ascorbic acid Sigma Cat. #95210 Ammonium iron (II) sulfate Sigma Cat. #215406 Tween 20 Biosharp Cat. #BS100 Sodium acetate solution (pH 4.0) Prepared in-house N/A Pic-borane (pyridine borane) Alfa Aesar Cat. #H66642 EDTA (in blood collection tubes) BD Bioscience N/A mTET2 enzyme (recombinant dioxygenase) Vazyme Cat. #EM301 M.SssI methyltransferase New England Biolabs (NEB) Cat. #M0226L Tris-HCl (pH 8.0) Biosharp Cat. #BL538A HpaII New England Biolabs (NEB) Cat. #R0171 Critical commercial assays High Sensitivity D1000 DNA ScreenTape Agilent Technologies Cat. #5067–5584 High Sensitivity D1000 Reagents Agilent Technologies Cat. #5067–5585 High Sensitivity D5000 DNA ScreenTape Agilent Technologies Cat. # 5067–5592 High Sensitivity D5000 Reagents Agilent Technologies Cat. #5067–5593 Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat. #Q32854 Qubit RNA HS Assay Kit Thermo Fisher Scientific Cat. #Q32855 SMARTer RNA Unique Dual Index Kit-96U Takara Bio Cat. #634452 SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian Takara Bio Cat. #634413 VAHTS Serum/Plasma Circulating DNA Kit Vazyme Cat. #N902 AMPure XP beads Beckman Coulter Cat. #A63881 VAHTS Unique Dual Index Adaptor for Illumina Vazyme Cat. #N34201 VAHTS Universal DNA Library Prep Kit for Illumina V3 Vazyme Cat. #ND607 VAHTS HiFi amplification mix V3 Vazyme Cat. #NE103-P1 VAHTS Library Quantification Kit for Illumina Vazyme Cat. #NQ107-01 Phusion High-Fidelity DNA Polymerase New England Biolabs (NEB) Cat. #M0531 RNAiso Plus Takara Bio Cat. #9109 Oligo Clean & Concentrator-5 kit Zymo Research Cat. #D4061 (Continued on next page) Cell Reports Medicine 7, 103075, October 20, 2026 e1 Article ll OPEN ACCESS

    Sequencing:

    Article Title: Pseudomonas aeruginosa induces the generation of pathogenic IFNγ + Th17 cells that promote lung damage and chronic inflammation in cystic fibrosis.
    Article Snippet: Total RNAs were extracted using the kit Quick-RNA MicroPrep (Zymo Research), the quality of RNAs was evaluated using TapeStation 4200 (Agilent) and only RNAs showing an RNA integrity number > 7 were used for library preparation. .. Sequencing libraries were prepared using the SMART Seq v4 PLUS kit (Takara) starting from 5 ng total RNA per sample and sequenced with the NovaSeq6000 (Illumina), generating on average 57.6 million 100-bp paired-end reads per sample. ..

    Article Title: Switching of transcriptional control from interferon regulatory factor 2 to interferon regulatory factor 1 drives innate immune cell activation.
    Article Snippet: Total RNA was quantified with the Qubit RNA HS Assay Kit (ThermoFisher, Q32852) and assessed using the RNA ScreenTape on TapeStation 4200 (Agilent Technologies). .. Sequencing libraries were generated using the Truseq Stranded mRNA kit (Illumina, 20020594) with an input of 100-1,000 ng of total RNA (for BMDM experiments), or the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634411) with 1-10 ng of total RNA (for skin or spleen experiments). .. Libraries were quantified with the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851) and the average library size was determined using the D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).

    Article Title: Tumor-associated calcium signal transducer 2 expression as a promising therapeutic target in esophageal squamous cell carcinoma: a combined the Cancer Genome Atlas and an institutional cohort.
    Article Snippet: Briefly, total RNA was extracted from two FFPE tissue slices using an miRNeasy FFPE kit (Qiagen) [20]. .. Whole-transcriptome sequencing was performed using the SMART-Seq Stranded kit (Takara Bio Europe) for library preparation and NextSeq 500, single end 75 bp (Illumina) for sequencing [21]. ..

    Article Title: A multi-omics analysis of advanced triple-negative breast cancer identifies predictors to chemo-immunotherapy and on-treatment dynamics.
    Article Snippet: .. CeGaT used KAPA RNA Hyper Prep (Roche) or SMART-Seq Stranded (Takara Bio) for the library preparation and sequencing on the NovaSeq6000, at 2 × 100 bp, at a depth of 100 Mio paired end reads. ..

    RNA Sequencing:

    Article Title: A collaborative study evaluating the detection of virus-infected cells by transcriptomic high-throughput sequencing.
    Article Snippet: RNA integrity was evaluated with 2100 Bioanalyzer using RNA 6000 Pico Kit (Agilent technologies, Cat. #5067-1513). .. Library preparation was carried out using SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara, Cat. #634418) and its corresponding index: SMARTer RNA Unique Dual Index Kit (Takara, Cat. #634452). .. AMPure XP beads (Beckman Coulter, Cat. #A63881) were used for library cleanup according to the manufacturer’s instructions to obtain a final volume of 20 μL.

    Article Title: Switching of transcriptional control from interferon regulatory factor 2 to interferon regulatory factor 1 drives innate immune cell activation.
    Article Snippet: Total RNA was quantified with the Qubit RNA HS Assay Kit (ThermoFisher, Q32852) and assessed using the RNA ScreenTape on TapeStation 4200 (Agilent Technologies). .. Sequencing libraries were generated using the Truseq Stranded mRNA kit (Illumina, 20020594) with an input of 100-1,000 ng of total RNA (for BMDM experiments), or the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634411) with 1-10 ng of total RNA (for skin or spleen experiments). .. Libraries were quantified with the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851) and the average library size was determined using the D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).

    Generated:

    Article Title: Switching of transcriptional control from interferon regulatory factor 2 to interferon regulatory factor 1 drives innate immune cell activation.
    Article Snippet: Total RNA was quantified with the Qubit RNA HS Assay Kit (ThermoFisher, Q32852) and assessed using the RNA ScreenTape on TapeStation 4200 (Agilent Technologies). .. Sequencing libraries were generated using the Truseq Stranded mRNA kit (Illumina, 20020594) with an input of 100-1,000 ng of total RNA (for BMDM experiments), or the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634411) with 1-10 ng of total RNA (for skin or spleen experiments). .. Libraries were quantified with the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851) and the average library size was determined using the D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).



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