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ion total rna seq kit v2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ion total rna seq kit v2
    Ion Total Rna Seq Kit V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seq/nct04488614-6-19-24
    Average 86 stars, based on 1 article reviews
    ion total rna seq kit v2 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Prospective Breast Cancer Biobanking Project
    Article Snippet: .. MiRNA and mRNA will be profiled using PureLinkTM miRNA Isolation Kit (Invitrogen), Dynabeads mRNA direct micro kit (Ambion),) and Ion total RNA-seq kit V2 (Life Technologies) on our Ion Proton NGS instrument. ..

    RNA Sequencing:

    Article Title: Prospective Breast Cancer Biobanking Project
    Article Snippet: .. MiRNA and mRNA will be profiled using PureLinkTM miRNA Isolation Kit (Invitrogen), Dynabeads mRNA direct micro kit (Ambion),) and Ion total RNA-seq kit V2 (Life Technologies) on our Ion Proton NGS instrument. ..

    Next-Generation Sequencing:

    Article Title: Prospective Breast Cancer Biobanking Project
    Article Snippet: .. MiRNA and mRNA will be profiled using PureLinkTM miRNA Isolation Kit (Invitrogen), Dynabeads mRNA direct micro kit (Ambion),) and Ion total RNA-seq kit V2 (Life Technologies) on our Ion Proton NGS instrument. ..

    other:

    Article Title: Mining differentially expressed genes during paratomy in the transcriptome of the flatworm Stenostomum leucops
    Article Snippet: For library preparation, mRNA was enriched using the Dynabeads ® mRNA purification kit (Invitrogen) and library preparation was performed using the Total Ion RNA-Seq v2 kit (Thermo Fisher Scientific).

    Article Title: Improved Predictability of Diagnosis and Prognosis Using Serum‐ and Tissue‐Derived Extracellular Vesicles From Bulk mRNA Sequencing in Pancreatic Ductal Adenocarcinoma
    Article Snippet: The total RNA of Ti‐EVs and Se‐EVs was isolated and purified via Ion Total RNA‐Seq Kit V2 (Life Technologies 4,475,936) by observing the kit's descriptions.

    Article Title: RNA stability is regulated by both RNA polyadenylation and ATP levels, linking RNA and energy metabolisms in Escherichia coli
    Article Snippet: Subsequently, 10 ng ribodepleted RNA was further used to construct a sequencing library using Ion Total RNA-Seq Kit v2 and Ion Xpres RNA-Seq Barcode Kit (Thermo Fisher Scientific).

    Article Title: Serotonergic-dependent effects of exercise and elevated stress hormone on small non-coding RNA transcriptomics and proteomics in a mouse model of affective disorders.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Improved Predictability of Diagnosis and Prognosis Using Serum- and Tissue-Derived Extracellular Vesicles From Bulk mRNA Sequencing in Pancreatic Ductal Adenocarcinoma.
    Article Snippet: The total RNA of Ti- EVs and Se- EVs was isolated and purified via Ion Total RNA- Seq Kit V2 (Life Technologies 4,475,936) by observing the kit's descriptions.

    Article Title: Regulatory Analysis of Root Architectural and Anatomical Adaptation to Nitrate and Ammonium in Brachypodium distachyon
    Article Snippet: The extracted RNA was sequenced using Illumina technology, and sequencing libraries were prepared with the Ion Total RNA-Seq Kit v2 following the protocol from Life Technologies, USA.

    Article Title: Distinct transcriptome and traits of freshly dispersed Pseudomonas aeruginosa cells
    Article Snippet: For library construction, the Ion Total RNA-Seq kit v2 (Life Technologies, Carlsbad, CA) was employed, and the libraries were evaluated using a Qubit 2 Fluorometer (Thermo Scientific, MA, USA).



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    SAHA rescues chromatin and transcriptomic changes (A) Outline of the experimental strategy. (B) PCA plot of ATAC-seq data obtained from four WT, five TS mice, and three TS-SAHA (TST). (C) PCA plot <t>of</t> <t>RNA-seq</t> data obtained from four WT and four TS mice and three TS-SAHA (TST). (D) Number of differentially accessible regions (DARs) for each comparison (left), and number of DARs in non-triplicated and triplicated genomic regions (right). (E) Number of differentially expressed genes (DEGs) for each comparison (left) and number of DEGs in non-triplicated and triplicated genomic regions (right). (F) Analysis of differentially accessible regions (DARs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (left). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (G) Analysis of differentially expressed genes (DEGs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (right). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (H) Heatmap of transcription factor motif enrichment among gained peaks in TS and TS-SAHA neurons, with significance and fold enrichment. (I) Gene set enrichment analysis of differentially expressed genes in TS (red) and TS-SAHA neurons (orange). See also .
    Smarter Stranded Total Rna Seq Kit V2 Pico Input Mammalian, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SAHA rescues chromatin and transcriptomic changes (A) Outline of the experimental strategy. (B) PCA plot of ATAC-seq data obtained from four WT, five TS mice, and three TS-SAHA (TST). (C) PCA plot <t>of</t> <t>RNA-seq</t> data obtained from four WT and four TS mice and three TS-SAHA (TST). (D) Number of differentially accessible regions (DARs) for each comparison (left), and number of DARs in non-triplicated and triplicated genomic regions (right). (E) Number of differentially expressed genes (DEGs) for each comparison (left) and number of DEGs in non-triplicated and triplicated genomic regions (right). (F) Analysis of differentially accessible regions (DARs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (left). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (G) Analysis of differentially expressed genes (DEGs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (right). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (H) Heatmap of transcription factor motif enrichment among gained peaks in TS and TS-SAHA neurons, with significance and fold enrichment. (I) Gene set enrichment analysis of differentially expressed genes in TS (red) and TS-SAHA neurons (orange). See also .
    Ion Total Rna Seq Kit V2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mcfdna seq  (Karius Inc)
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    SAHA rescues chromatin and transcriptomic changes (A) Outline of the experimental strategy. (B) PCA plot of ATAC-seq data obtained from four WT, five TS mice, and three TS-SAHA (TST). (C) PCA plot <t>of</t> <t>RNA-seq</t> data obtained from four WT and four TS mice and three TS-SAHA (TST). (D) Number of differentially accessible regions (DARs) for each comparison (left), and number of DARs in non-triplicated and triplicated genomic regions (right). (E) Number of differentially expressed genes (DEGs) for each comparison (left) and number of DEGs in non-triplicated and triplicated genomic regions (right). (F) Analysis of differentially accessible regions (DARs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (left). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (G) Analysis of differentially expressed genes (DEGs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (right). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (H) Heatmap of transcription factor motif enrichment among gained peaks in TS and TS-SAHA neurons, with significance and fold enrichment. (I) Gene set enrichment analysis of differentially expressed genes in TS (red) and TS-SAHA neurons (orange). See also .
    Mcfdna Seq, supplied by Karius Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SAHA rescues chromatin and transcriptomic changes (A) Outline of the experimental strategy. (B) PCA plot of ATAC-seq data obtained from four WT, five TS mice, and three TS-SAHA (TST). (C) PCA plot <t>of</t> <t>RNA-seq</t> data obtained from four WT and four TS mice and three TS-SAHA (TST). (D) Number of differentially accessible regions (DARs) for each comparison (left), and number of DARs in non-triplicated and triplicated genomic regions (right). (E) Number of differentially expressed genes (DEGs) for each comparison (left) and number of DEGs in non-triplicated and triplicated genomic regions (right). (F) Analysis of differentially accessible regions (DARs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (left). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (G) Analysis of differentially expressed genes (DEGs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (right). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (H) Heatmap of transcription factor motif enrichment among gained peaks in TS and TS-SAHA neurons, with significance and fold enrichment. (I) Gene set enrichment analysis of differentially expressed genes in TS (red) and TS-SAHA neurons (orange). See also .
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    Combining poly(A)+-selected and <t>rRNA-depleted</t> <t>RNA-seq</t> with manual re-annotation reclassified 423 Fumigati section-specific genes ( ssp ), revealing widespread non-coding ssp genes. Functional analyses identified two lncRNAs: SSP7 supports mitochondrial function by maintaining translation of pprA mRNA, whereas SSP8 interacts with amino acid-related proteins to regulate amino acid metabolism. Together, these lncRNA-mediated pathways promote fungal development and virulence. Created in BioRender. Zhang, Y. (2026) https://BioRender.com/47wpmko .
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    Combining poly(A)+-selected and <t>rRNA-depleted</t> <t>RNA-seq</t> with manual re-annotation reclassified 423 Fumigati section-specific genes ( ssp ), revealing widespread non-coding ssp genes. Functional analyses identified two lncRNAs: SSP7 supports mitochondrial function by maintaining translation of pprA mRNA, whereas SSP8 interacts with amino acid-related proteins to regulate amino acid metabolism. Together, these lncRNA-mediated pathways promote fungal development and virulence. Created in BioRender. Zhang, Y. (2026) https://BioRender.com/47wpmko .
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    Image Search Results


    SAHA rescues chromatin and transcriptomic changes (A) Outline of the experimental strategy. (B) PCA plot of ATAC-seq data obtained from four WT, five TS mice, and three TS-SAHA (TST). (C) PCA plot of RNA-seq data obtained from four WT and four TS mice and three TS-SAHA (TST). (D) Number of differentially accessible regions (DARs) for each comparison (left), and number of DARs in non-triplicated and triplicated genomic regions (right). (E) Number of differentially expressed genes (DEGs) for each comparison (left) and number of DEGs in non-triplicated and triplicated genomic regions (right). (F) Analysis of differentially accessible regions (DARs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (left). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (G) Analysis of differentially expressed genes (DEGs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (right). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (H) Heatmap of transcription factor motif enrichment among gained peaks in TS and TS-SAHA neurons, with significance and fold enrichment. (I) Gene set enrichment analysis of differentially expressed genes in TS (red) and TS-SAHA neurons (orange). See also .

    Journal: iScience

    Article Title: HDAC inhibitor treatment restores transcriptome, chromatin accessibility, and memory deficits in a mouse model for Down syndrome

    doi: 10.1016/j.isci.2026.117670

    Figure Lengend Snippet: SAHA rescues chromatin and transcriptomic changes (A) Outline of the experimental strategy. (B) PCA plot of ATAC-seq data obtained from four WT, five TS mice, and three TS-SAHA (TST). (C) PCA plot of RNA-seq data obtained from four WT and four TS mice and three TS-SAHA (TST). (D) Number of differentially accessible regions (DARs) for each comparison (left), and number of DARs in non-triplicated and triplicated genomic regions (right). (E) Number of differentially expressed genes (DEGs) for each comparison (left) and number of DEGs in non-triplicated and triplicated genomic regions (right). (F) Analysis of differentially accessible regions (DARs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (left). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (G) Analysis of differentially expressed genes (DEGs) in TS neurons and their rescue upon SAHA treatment, shown as line-plots (left) and Venn diagram (right). Kruskal-Wallis test followed by pairwise Wilcoxon tests with multiple-comparison correction, ∗∗∗ p < 0.001. (H) Heatmap of transcription factor motif enrichment among gained peaks in TS and TS-SAHA neurons, with significance and fold enrichment. (I) Gene set enrichment analysis of differentially expressed genes in TS (red) and TS-SAHA neurons (orange). See also .

    Article Snippet: SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian , Takara , Cat#634411.

    Techniques: RNA Sequencing, Comparison, Analysis

    Journal: iScience

    Article Title: HDAC inhibitor treatment restores transcriptome, chromatin accessibility, and memory deficits in a mouse model for Down syndrome

    doi: 10.1016/j.isci.2026.117670

    Figure Lengend Snippet:

    Article Snippet: SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian , Takara , Cat#634411.

    Techniques: Cell-Signaling, Recombinant, Bicinchoninic Acid Protein Assay, Isolation, DNA Library Preparation, Software, Analysis

    Combining poly(A)+-selected and rRNA-depleted RNA-seq with manual re-annotation reclassified 423 Fumigati section-specific genes ( ssp ), revealing widespread non-coding ssp genes. Functional analyses identified two lncRNAs: SSP7 supports mitochondrial function by maintaining translation of pprA mRNA, whereas SSP8 interacts with amino acid-related proteins to regulate amino acid metabolism. Together, these lncRNA-mediated pathways promote fungal development and virulence. Created in BioRender. Zhang, Y. (2026) https://BioRender.com/47wpmko .

    Journal: PLOS Pathogens

    Article Title: Large-scale genome re-annotation uncovers pathogen-specific lncRNA regulators of fungal growth and virulence

    doi: 10.1371/journal.ppat.1014681

    Figure Lengend Snippet: Combining poly(A)+-selected and rRNA-depleted RNA-seq with manual re-annotation reclassified 423 Fumigati section-specific genes ( ssp ), revealing widespread non-coding ssp genes. Functional analyses identified two lncRNAs: SSP7 supports mitochondrial function by maintaining translation of pprA mRNA, whereas SSP8 interacts with amino acid-related proteins to regulate amino acid metabolism. Together, these lncRNA-mediated pathways promote fungal development and virulence. Created in BioRender. Zhang, Y. (2026) https://BioRender.com/47wpmko .

    Article Snippet: Libraries were then prepared using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (NR605, Vazyme) according to the manufacturer’s instructions.

    Techniques: RNA Sequencing, Functional Assay