Journal: EMBO Reports
Article Title: Transposable element activity captures human pluripotent cell states
doi: 10.1038/s44319-024-00343-y
Figure Lengend Snippet: Reagents and tools table
Article Snippet: 5i/LAF medium contained a 1:1 mixture of DMEM/F-12 (Sigma) and Neurobasal medium (Thermo Fisher) containing 1X N2 supplement (Thermo Fisher), 1X B27 supplement (Thermo Fisher), 10 ng/mL bFGF (Peprotech), 1% nonessential amino acids (Sigma-Aldrich), 1 mM l -glutamine (Sigma-Aldrich), penicillin-streptomycin (Sigma-Aldrich), 0.1 mM β-mercaptoethanol (Thermo Fisher), 50 μg/mL BSA (Sigma-Aldrich), 0.5 μM IM-12 (Axon Medchem), 0.5 μM SB590885 (Axon Medchem), 1 μM WH-4-023 (Axon Medchem), 10 μM Y-27632 (Axon Medchem), 20 ng/mL Activin A (Peprotech), 20 ng/mL rhLIF (Peprotech), 0.5% KSR (Gibco), and 1 μM PD0325901 (Axon Medchem).
Techniques: Recombinant, Modification, Purification, Sequencing, Knock-Out, Software, Electroporation