sb20358  (MedChemExpress)

Journal: Frontiers in Immunology

Article Title: Targeting the oxidative stress-neuroinflammation axis: the mechanism of arctigenin’s broad-spectrum analgesia with limited side effects

doi: 10.3389/fimmu.2026.1754756

Figure Lengend Snippet: The antiallodynic effects of AG in SNI mice are associated with MAPK signaling pathway. (A) A shows western blotting of p-ERK and t-ERK in each group of spinal cord and qualitative data of protein expression in different groups. (B) Western blotting of p-JNK and t-JNK in different groups. (C) Western blotting of p-p38 and t-p38 in different groups. (D) Western blotting of p-mTOR in different groups. (E) Western blotting of PGC-a in different groups. (F) Western blotting of p-AMPK in different groups. (G) The analgesic effects of AG in SNI mice could be blocked by pretreatment with inhibitors of the MAPK pathway [U0126 (100 μg/kg, i.p.), SB203580 (40 μg/ml, 10 ml/kg, i.p.), or SP600125 (50 μg/kg, i.p.)], as measured by von Frey testing. (H) The extent and duration of analgesia are estimated by the area under curve (AUC (g min)) of PWT vs time (0-120 minutes). Data are expressed as the mean ± SEM. ** p < 0.01 and *** p < 0.001 for sham group vs SNI group. # p < 0.05, and ## p < 0.01 for SNI group vs AG+SNI group. && p < 0.01 compared with AG+SNI group. The "ns" indicates no significant difference.

Article Snippet: U0126 (inhibitor of ERK, cat number: HY-12031A), SB20358 (inhibitor of p38, cat number: HY-112349) and SP600125 (inhibitor of JNK, cat number: HY-12041) were purchased from MedChemExpress.

Techniques: Western Blot, Expressing

Journal: Bioengineered

Article Title: Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis

doi: 10.1080/21655979.2022.2056690

Figure Lengend Snippet: Protein levels of p-EKR1/2, p-P38 and p-JNK in RAW264.7 and Ana-1 cells. *P < 0.05 vs. Control group, # P < 0.05 vs. AEm group.

Article Snippet: The RhoA inhibitor Y-27632 (HY-10071), JNK inhibitor SP600125 (HY-12041), ERK inhibitor U0126-EtOH (HY-12031), and p38 inhibitor SB20358 (HY-10256) were obtained from MedChemExpress (MCE).

Techniques: Control

Journal: Frontiers in cellular and infection microbiology

Article Title: Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization.

doi: 10.3389/fcimb.2017.00078

Figure Lengend Snippet: FIGURE 3 | p38, JNK, and ERK1/2 activation regulated by NaO in S. aureus-challenged bMECs. MAPK phosphorylation was measured in bMECs that were treated with 0.25 or 1 mM NaO and/or challenged with S. aureus by flow cytometry. The phosphorylated MAPK concentrations (U/ml) are represented: (A) pp38, (B) pJNK1/2, and (C) pERK1/2. Each bar shows the result of one experiment. SB203580: p38 inhibitor. SP600125: JNK1/2 inhibitor. U0126: ERK1/2 inhibitor. Different letters above the bars indicate significant changes among the unchallenged bMECs (white bars) and S. aureus-challenged cells within the same MAPK evaluated (p < 0.05).

Article Snippet: TheMAPK inhibitors SB20358 (p38), SP600125 (JNK), and U0126 (ERK1/2) were acquired from Cell Signaling Technology R© (Boston, MA).

Techniques: Activation Assay, Phospho-proteomics, Cytometry