Transplantation Assay:Article Title: Treatment of Acute Liver Failure in Mice by Hepatocyte Xenotransplantation
Article Snippet: .. As shown in Figure 1, the experimental groups were divided into the following five groups: group 1: trans-Here we have demonstrated that SAPNF has an ex- cellent ability to promote hepatocyte engraftment, main- plantation of 200 μl of 0.5% SAPNF-suspended porcine hepatocytes (1 × 106) (n = 10); group 2: transplantationtaining tremendous hepatocyte functions capable of res- cuing mice from ALF in a xenotransplantation model. of 200 μl of collagen-suspended porcine hepatocytes (1 × 106) (n = 10); group 3: transplantation of porcine micrometer sections were prepared for immunofluorescence staining for porcine albumin expression using goathepatocytes alone resuspended in 200 μl of medium (1 × 106) (n = 10); group 4: transplantation of cellular anti-pig albumin antibody (Bethyl, Laboratories Inc.) and followed by FITC-conjugated mouse anti-goat IgGhomogenates (1 × 106) (n = 10); group 5: transplantation of 200 μl of 0.5% SAPNF alone without hepatocytes (Sigma). .. Localization of albumin was visualized by an Axiophot FL fluorescence microscope (CarlZeiss). (n = 10).
Immunofluorescence:Article Title: Treatment of Acute Liver Failure in Mice by Hepatocyte Xenotransplantation
Article Snippet: .. As shown in Figure 1, the experimental groups were divided into the following five groups: group 1: trans-Here we have demonstrated that SAPNF has an ex- cellent ability to promote hepatocyte engraftment, main- plantation of 200 μl of 0.5% SAPNF-suspended porcine hepatocytes (1 × 106) (n = 10); group 2: transplantationtaining tremendous hepatocyte functions capable of res- cuing mice from ALF in a xenotransplantation model. of 200 μl of collagen-suspended porcine hepatocytes (1 × 106) (n = 10); group 3: transplantation of porcine micrometer sections were prepared for immunofluorescence staining for porcine albumin expression using goathepatocytes alone resuspended in 200 μl of medium (1 × 106) (n = 10); group 4: transplantation of cellular anti-pig albumin antibody (Bethyl, Laboratories Inc.) and followed by FITC-conjugated mouse anti-goat IgGhomogenates (1 × 106) (n = 10); group 5: transplantation of 200 μl of 0.5% SAPNF alone without hepatocytes (Sigma). .. Localization of albumin was visualized by an Axiophot FL fluorescence microscope (CarlZeiss). (n = 10).
Staining:Article Title: Treatment of Acute Liver Failure in Mice by Hepatocyte Xenotransplantation
Article Snippet: .. As shown in Figure 1, the experimental groups were divided into the following five groups: group 1: trans-Here we have demonstrated that SAPNF has an ex- cellent ability to promote hepatocyte engraftment, main- plantation of 200 μl of 0.5% SAPNF-suspended porcine hepatocytes (1 × 106) (n = 10); group 2: transplantationtaining tremendous hepatocyte functions capable of res- cuing mice from ALF in a xenotransplantation model. of 200 μl of collagen-suspended porcine hepatocytes (1 × 106) (n = 10); group 3: transplantation of porcine micrometer sections were prepared for immunofluorescence staining for porcine albumin expression using goathepatocytes alone resuspended in 200 μl of medium (1 × 106) (n = 10); group 4: transplantation of cellular anti-pig albumin antibody (Bethyl, Laboratories Inc.) and followed by FITC-conjugated mouse anti-goat IgGhomogenates (1 × 106) (n = 10); group 5: transplantation of 200 μl of 0.5% SAPNF alone without hepatocytes (Sigma). .. Localization of albumin was visualized by an Axiophot FL fluorescence microscope (CarlZeiss). (n = 10).
Expressing:Article Title: Treatment of Acute Liver Failure in Mice by Hepatocyte Xenotransplantation
Article Snippet: .. As shown in Figure 1, the experimental groups were divided into the following five groups: group 1: trans-Here we have demonstrated that SAPNF has an ex- cellent ability to promote hepatocyte engraftment, main- plantation of 200 μl of 0.5% SAPNF-suspended porcine hepatocytes (1 × 106) (n = 10); group 2: transplantationtaining tremendous hepatocyte functions capable of res- cuing mice from ALF in a xenotransplantation model. of 200 μl of collagen-suspended porcine hepatocytes (1 × 106) (n = 10); group 3: transplantation of porcine micrometer sections were prepared for immunofluorescence staining for porcine albumin expression using goathepatocytes alone resuspended in 200 μl of medium (1 × 106) (n = 10); group 4: transplantation of cellular anti-pig albumin antibody (Bethyl, Laboratories Inc.) and followed by FITC-conjugated mouse anti-goat IgGhomogenates (1 × 106) (n = 10); group 5: transplantation of 200 μl of 0.5% SAPNF alone without hepatocytes (Sigma). .. Localization of albumin was visualized by an Axiophot FL fluorescence microscope (CarlZeiss). (n = 10).
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