saos2 cells  (ATCC)

Journal: BMC Medicine

Article Title: Oncostatin-M ligand-based CAR-T therapy displays robust anti-tumor activity against osteosarcoma

doi: 10.1186/s12916-026-04729-8

Figure Lengend Snippet: Intravenously (IV) injected OSM-CAR-T cells display cytotoxicity against subcutaneous SAOS2. A Shows the schematic of the experiment with the number of T-cells injected. B Displays photo of extracted tumors four days after T-cell injection. C Shows mass of extracted SAOS2 tumors 5 days after IV injection of 6,000,000 T-cells or vehicle. D Shows volume of extracted tumors measured as L×W×D× (π/6) 5 days after injection. E Displays human cytokine multiplex results from 2 mice run in duplicate. F Displays mouse cytokine multiplex results from 2 mice run in duplicate. UT stands for untransduced T-cells that were activated and expanded in the same way as CAR T-cells. Null stands for the blank wells serving as a null control. One-way ANOVA performed with Tukey’s post hoc test for multiple comparisons performed for comparisons of three or more groups. Only relevant comparisons shown for clarity. p -values shown as numeric values. Sample size of the full experiment was 5 mice for the PBS group and 6 mice for the UT and CAR-T groups

Article Snippet: 143B and SAOS2 cell lines were purchased from ATCC originally by collaborators and cultured in DMEM 10% FBS and 1% Pen/Strep.

Techniques: Injection, IV Injection, Multiplex Assay, Control

Journal: BMC Medicine

Article Title: Oncostatin-M ligand-based CAR-T therapy displays robust anti-tumor activity against osteosarcoma

doi: 10.1186/s12916-026-04729-8

Figure Lengend Snippet: OSM-CAR-T-cells display specificity to OSMR-expressing cell lines. A Schematic of experiment; Jeko cells were injected at a 1:1 ratio of PBS to Matrigel and allowed to grow for 14 days prior to injection of T-cells or vehicle. B Luciferase-tagged Jeko cells fluorescence was measured prior to injection of T-cells IV (day 0) and at day 7 and 14 via Spectrum Imaging System. C Luciferase imaging values (radiance) displayed over time. D Radiance of tumors 14 days after T-cell injection. E Image of the extracted tumors is displayed. 3 mice were used per group. F Schematic of “safety” experiment where T-cells were injected into an NSG mouse with no tumor burden. G Displays the change in mass of the mice, where day 0 is the day T-cells were injected IV. H Human cytokine multiplex results 10 days after T-cell injection. WT represents a non-injected NSG mouse. UT represents untransduced T-cells and CAR stands for OSM-CAR-T-cells. I Mouse cytokine multiplex results 10 days after T-cell injection. Null represents blank controls used in assay. J Cryoviz computer representation of whole sectioned mouse with 10 day old SAOS2-mNeon tagged subcutaneous tumor. Yellow dots are Q-tracker labeled T-cells 16 h after injection of OSM-CAR-T-cells. K Location and tumor infiltration represented in yellow dots on tumor (green). Orange arrow points to collection of CAR T-cells within tumor 16 h after injection. L Graphical representation of computer quantification of yellow signal representing Q-tracker labeled CAR-T-cells. Ordinary one-way ANOVA with Tukey’s multiple comparison test performed for groups of three or more. For comparison between two groups, an unpaired Student’s T -test was performed. p -values are displayed as numerical values

Article Snippet: 143B and SAOS2 cell lines were purchased from ATCC originally by collaborators and cultured in DMEM 10% FBS and 1% Pen/Strep.

Techniques: Expressing, Injection, Luciferase, Fluorescence, Imaging, Multiplex Assay, Labeling, Comparison