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sal003  (MedChemExpress)
93
MedChemExpress sal003
A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
Sal003, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sal003 hy15869  (MedChemExpress)
93
MedChemExpress sal003 hy15869
A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
Sal003 Hy15869, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
sal003 hy15869 - by Bioz Stars, 2026-03
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neurons  (MedChemExpress)
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MedChemExpress neurons
A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
Neurons, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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afatinib  (MedChemExpress)
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MedChemExpress afatinib
A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
Afatinib, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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afatinib - by Bioz Stars, 2026-03
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medchemexpress sal003  (MedChemExpress)
93
MedChemExpress medchemexpress sal003
A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM <t>Sal003</t> (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.
Medchemexpress Sal003, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
medchemexpress sal003 - by Bioz Stars, 2026-03
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A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM Sal003 (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.

Journal: bioRxiv

Article Title: The Integrated Stress Response Pathway Improves Aged Murine Muscle Stem Cell Activation and in vivo Regeneration

doi: 10.1101/2025.09.26.678822

Figure Lengend Snippet: A. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, 10 µM Sal003 (ISR-a) or 200 nM ISRIB (ISR-i), pulsed with EdU and analyzed at 48 hours in culture (top); Q = quiescent, A = activated. Percentage of Pax7 + EdU + cells at 48 hours after ISR-a (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=3; *p<0.05. B. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a or ISR-i for 48 hours (top). Percentage of Casp3 + myogenic cells at 48 hours after treatment (bottom). Mean±SEM; n=3; ***p<0.001. C. Schematic of single muscle fibers from adult mice (3-6 months) treated either with DMSO, ISR-a or ISR-i and cultured for 72 hours in culture (top); Q = quiescent, A = activated, D = differentiated. Percentage of Pax7 - /MyoG + (orange) and Pax7 + /MyoG - (purple) cells at 72 hours in culture after ISR-a treatment (bottom left) and ISR-i treatment (bottom right). Mean±SEM; n=2, *p<0.05. D. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, 10 µM rapamycin, ISR-a or ISR-i for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, Rapamycin, ISR-a or ISR-i treatment. Mean±SEM; n=3; *p<0.05; **p<0.01. E. Schematic of FACS sorted adult MuSCs in vitro cultured in DMSO, ISR-a, 100 µg/ml cycloheximide (CHX), or CHX+ISR-a for 48 hours (top); Q = quiescent, A = activated. Percentage of EdU + FACS sorted myogenic cells at 48 hours after DMSO, ISR-a, CHX, or CHX+ISR-a treatment. Mean±SEM; n=3; *p<0.05; **p<0.01, ***p<0.001. F. Schematic of FACS sorted adult MuSCs in vitro cultured in ISR-a pulsed with EdU and ATF4 shRNA for 48 hours (top). Percentage of Pax7 + EdU + cells at 48 hours after ISR-a, ATF4 shRNA or with ISR-a+ATF4 shRNA (bottom). Mean±SEM; n=3; *p<0.05.

Article Snippet: Adult (4 month) and aged (20-22 month) C57 mice were administered 1-hour pre-BaCl 2 injury via IP either Sal003 (HY-15969, MedChemExpress, 1 mg/kg) or ISRIB (HY-12459, MedChemExpress, 1 mg/kg), both dissolved in DMSO and PBS.

Techniques: In Vitro, Cell Culture, shRNA