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Proteintech sa00003
Sa00003, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sa00003/Goat+anti-mouse+IgG+(H%2BL)%2C+FITC+conjugate/pm42782103-104-11-10
Average 96 stars, based on 389 article reviews
sa00003 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Incubation:

Article Title: The ARHGAP10-202aa protein encoded by circARHGAP10 promotes skeletal muscle development and regeneration.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: The CDO1–ACSM3 Axis Mediates Renal Tubule Lipid Deposition and Injury by Causing Mitochondrial Dysfunction in Lupus Nephritis
Article Snippet: The sections were then blocked with 10% goat serum and incubated with primary antibodies against CDO1 (1:200, Proteintech, 12589-1-AP), AQP1 (1:200, Proteintech, 66805-1-Ig), and COX IV (1:1000, Abcam, ab33985, Cambridge, MA, USA). .. Following incubation with TRITC-conjugated goat anti-rabbit IgG (H + L) (1:200, Proteintech, SA00007-2), FITC-conjugated goat anti-mouse IgG (H + L) (1:200, Proteintech, SA00003-1), and DAPI (SouthernBiotech, Birmingham, AL, USA), fluorescence images were captured using a laser scanning confocal microscope (Leica, Wetzlar, Germany). .. Twenty-six-week-old (weighing 25–30 g) female MRL/lpr lupus-prone mice and age- and sex-matched MRL/MPJ mice were obtained from Huachuang Xinnuo Biotechnology (Taizhou City, Jiangsu Province, China).

Article Title: The CDO1-ACSM3 Axis Mediates Renal Tubule Lipid Deposition and Injury by Causing Mitochondrial Dysfunction in Lupus Nephritis.
Article Snippet: The sections were then blocked with 10% goat serum and incubated with primary antibodies against CDO1 (1:200, Proteintech, 12589-1-AP), AQP1 (1:200, Proteintech, 66805-1-Ig), and COX IV (1:1000, Abcam, ab33985, Cambridge, MA, USA). .. Following incubation with TRITC-conjugated goat anti-rabbit IgG (H + L) (1:200, Proteintech, SA00007-2), FITC-conjugated goat anti-mouse IgG (H + L) (1:200, Proteintech, SA00003-1), and DAPI (SouthernBiotech, Birmingham, AL, USA), fluorescence images were captured using a laser scanning confocal microscope (Leica, Wetzlar, Germany). .. Twenty-six-week-old (weighing 25–30 g) female MRL/lpr lupus-prone mice and ageand sex-matched MRL/MPJ mice were obtained from Huachuang Xinnuo Biotechnology (Taizhou City, Jiangsu Province, China).

Article Title: BMSC-EVs improve post-stroke cognition by promoting regionally distinct synaptic repair via Sema3G-Nrp2/PlexinA4 Signaling.
Article Snippet: Cognitive impairment is a common sequela of ischemic stroke, primarily driven by disrupted synaptic structural plasticity in the hippocampus.. Although bone marrow mesenchymal stem cell-derived extracellular vesicles (BMSC-EVs) are known to promote synaptic plasticity, their heterogeneous effects across hippocampal subregions and associated regulatory mechanisms remain unclear.. In this study, BMSC-EVs were intravenously administered 24 h post-reperfusion in a rat model of transient middle cerebral artery occlusion (tMCAO), with additional injections on days 3, 5, and 7.

Article Title: FBP1/HIF-1α Axis mediates macrophage metabolic reprogramming and serves as diagnostic biomarkers in atherosclerosis.
Article Snippet: Atherosclerosis (AS),a major global cause of cardiovascular mortality,is characterized by significant metabolic reprogramming of plaque macrophages in response to a hostile microenvironment containing cytokines, oxidized lipids, and hypoxia.. Macrophage metabolic reprogramming, marked by shifts in glycolysis, fatty acid, and amino acid metabolism has emerged as a critical contributor to chronic inflammatory diseases.. This study explores the role of fructose-1,6-bisphosphatase 1 (FBP1) in this process and its functional interplay with hypoxia-inducible factor-1α (HIF-1α).

Fluorescence:

Article Title: The CDO1–ACSM3 Axis Mediates Renal Tubule Lipid Deposition and Injury by Causing Mitochondrial Dysfunction in Lupus Nephritis
Article Snippet: The sections were then blocked with 10% goat serum and incubated with primary antibodies against CDO1 (1:200, Proteintech, 12589-1-AP), AQP1 (1:200, Proteintech, 66805-1-Ig), and COX IV (1:1000, Abcam, ab33985, Cambridge, MA, USA). .. Following incubation with TRITC-conjugated goat anti-rabbit IgG (H + L) (1:200, Proteintech, SA00007-2), FITC-conjugated goat anti-mouse IgG (H + L) (1:200, Proteintech, SA00003-1), and DAPI (SouthernBiotech, Birmingham, AL, USA), fluorescence images were captured using a laser scanning confocal microscope (Leica, Wetzlar, Germany). .. Twenty-six-week-old (weighing 25–30 g) female MRL/lpr lupus-prone mice and age- and sex-matched MRL/MPJ mice were obtained from Huachuang Xinnuo Biotechnology (Taizhou City, Jiangsu Province, China).

Article Title: The CDO1-ACSM3 Axis Mediates Renal Tubule Lipid Deposition and Injury by Causing Mitochondrial Dysfunction in Lupus Nephritis.
Article Snippet: The sections were then blocked with 10% goat serum and incubated with primary antibodies against CDO1 (1:200, Proteintech, 12589-1-AP), AQP1 (1:200, Proteintech, 66805-1-Ig), and COX IV (1:1000, Abcam, ab33985, Cambridge, MA, USA). .. Following incubation with TRITC-conjugated goat anti-rabbit IgG (H + L) (1:200, Proteintech, SA00007-2), FITC-conjugated goat anti-mouse IgG (H + L) (1:200, Proteintech, SA00003-1), and DAPI (SouthernBiotech, Birmingham, AL, USA), fluorescence images were captured using a laser scanning confocal microscope (Leica, Wetzlar, Germany). .. Twenty-six-week-old (weighing 25–30 g) female MRL/lpr lupus-prone mice and ageand sex-matched MRL/MPJ mice were obtained from Huachuang Xinnuo Biotechnology (Taizhou City, Jiangsu Province, China).

Microscopy:

Article Title: The CDO1–ACSM3 Axis Mediates Renal Tubule Lipid Deposition and Injury by Causing Mitochondrial Dysfunction in Lupus Nephritis
Article Snippet: The sections were then blocked with 10% goat serum and incubated with primary antibodies against CDO1 (1:200, Proteintech, 12589-1-AP), AQP1 (1:200, Proteintech, 66805-1-Ig), and COX IV (1:1000, Abcam, ab33985, Cambridge, MA, USA). .. Following incubation with TRITC-conjugated goat anti-rabbit IgG (H + L) (1:200, Proteintech, SA00007-2), FITC-conjugated goat anti-mouse IgG (H + L) (1:200, Proteintech, SA00003-1), and DAPI (SouthernBiotech, Birmingham, AL, USA), fluorescence images were captured using a laser scanning confocal microscope (Leica, Wetzlar, Germany). .. Twenty-six-week-old (weighing 25–30 g) female MRL/lpr lupus-prone mice and age- and sex-matched MRL/MPJ mice were obtained from Huachuang Xinnuo Biotechnology (Taizhou City, Jiangsu Province, China).

Article Title: The CDO1-ACSM3 Axis Mediates Renal Tubule Lipid Deposition and Injury by Causing Mitochondrial Dysfunction in Lupus Nephritis.
Article Snippet: The sections were then blocked with 10% goat serum and incubated with primary antibodies against CDO1 (1:200, Proteintech, 12589-1-AP), AQP1 (1:200, Proteintech, 66805-1-Ig), and COX IV (1:1000, Abcam, ab33985, Cambridge, MA, USA). .. Following incubation with TRITC-conjugated goat anti-rabbit IgG (H + L) (1:200, Proteintech, SA00007-2), FITC-conjugated goat anti-mouse IgG (H + L) (1:200, Proteintech, SA00003-1), and DAPI (SouthernBiotech, Birmingham, AL, USA), fluorescence images were captured using a laser scanning confocal microscope (Leica, Wetzlar, Germany). .. Twenty-six-week-old (weighing 25–30 g) female MRL/lpr lupus-prone mice and ageand sex-matched MRL/MPJ mice were obtained from Huachuang Xinnuo Biotechnology (Taizhou City, Jiangsu Province, China).



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GAC suppresses the viability of HCC cells in a ferroptosis-associated way. (A) Chemical structure of ginkgolic acid C17:1 (GAC). (B) Dose-dependent suppression of cell viability by GAC (0–20 μM, 24 h; CCK-8 assay), n = 3 each concentration. (C-D) Representative Annexin <t>V-FITC/PI</t> plots (A) and percentage of Annexin V + cells (D) in HCCLM3 and HepG2 cells treated by GAC or not, n = 3 in each group. (E-F) Suppressed clonogenic capacity: Representative colony formation images(E) and quantified colony counts (F), n = 3 in each group. (G-I) Western blot analysis of PCD-associated markers: representative immunoblots (G) and quantification of Caspase3 (H) and cleaved-Casepase3 (I), normalized to β-actin, n = 3 in each group. (J) CCK-8 assay assessing the effects of various programmed cell death (PCD) inhibitors on GAC-induced cytotoxicity, n = 3 in each group * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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Image Search Results


GAC suppresses the viability of HCC cells in a ferroptosis-associated way. (A) Chemical structure of ginkgolic acid C17:1 (GAC). (B) Dose-dependent suppression of cell viability by GAC (0–20 μM, 24 h; CCK-8 assay), n = 3 each concentration. (C-D) Representative Annexin V-FITC/PI plots (A) and percentage of Annexin V + cells (D) in HCCLM3 and HepG2 cells treated by GAC or not, n = 3 in each group. (E-F) Suppressed clonogenic capacity: Representative colony formation images(E) and quantified colony counts (F), n = 3 in each group. (G-I) Western blot analysis of PCD-associated markers: representative immunoblots (G) and quantification of Caspase3 (H) and cleaved-Casepase3 (I), normalized to β-actin, n = 3 in each group. (J) CCK-8 assay assessing the effects of various programmed cell death (PCD) inhibitors on GAC-induced cytotoxicity, n = 3 in each group * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Pharmaceutical Biology

Article Title: Ginkgolic acid targets HSPA8 to trigger ferroptosis in hepatocellular carcinoma via chaperone-mediated autophagy-dependent GPX4 degradation

doi: 10.1080/13880209.2026.2646350

Figure Lengend Snippet: GAC suppresses the viability of HCC cells in a ferroptosis-associated way. (A) Chemical structure of ginkgolic acid C17:1 (GAC). (B) Dose-dependent suppression of cell viability by GAC (0–20 μM, 24 h; CCK-8 assay), n = 3 each concentration. (C-D) Representative Annexin V-FITC/PI plots (A) and percentage of Annexin V + cells (D) in HCCLM3 and HepG2 cells treated by GAC or not, n = 3 in each group. (E-F) Suppressed clonogenic capacity: Representative colony formation images(E) and quantified colony counts (F), n = 3 in each group. (G-I) Western blot analysis of PCD-associated markers: representative immunoblots (G) and quantification of Caspase3 (H) and cleaved-Casepase3 (I), normalized to β-actin, n = 3 in each group. (J) CCK-8 assay assessing the effects of various programmed cell death (PCD) inhibitors on GAC-induced cytotoxicity, n = 3 in each group * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The following antibodies were used: anti-GPX4 (Proteintech, 67763-1-Ig, 1:500, AB_2909469), anti-HSPA8 (Huabio, ET1602-33,1:500, AB_3069646), anti-rabbit IgG FITC (Proteintech, SA00003-2, 1:1000, AB_2890897), anti-mouse IgG TRITC (Proteintech, SA00007-1, 1:1000, AB_2889940)

Techniques: CCK-8 Assay, Concentration Assay, Western Blot