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hrp conjugated secondary antibodies  (Proteintech)


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    Structured Review

    Proteintech hrp conjugated secondary antibodies
    Hrp Conjugated Secondary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 5898 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sa00001/Goat+anti-mouse+IgG+(H%2BL)%2C+HRP+conjugate/pm42826891-109-3-7
    Average 97 stars, based on 5898 article reviews
    hrp conjugated secondary antibodies - by Bioz Stars, 2026-10
    97/100 stars

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    other:

    Article Title: Knockdown of CCT2 inhibits the malignant progression of hepatocellular carcinoma cells by impairing STAT3 activation
    Article Snippet: SA00001-1 and SA00001-2; Proteintech Group, Inc.) for 1 h at room temperature.

    Article Title: Combining network pharmacology and experimental validation to study the action and mechanism of brusatol against lung adenocarcinoma.
    Article Snippet: The antibodies used were as follows: anti-Bax (Proteintech, 50599-2-lg), anti-Bcl2 (Proteintech, 12789-1-AP), anti-Cyclin B1 (Proteintech, 28603-1-AP), anti-P21(ZENBIO, 381102), anti-p-cdc2 (Tyr15) (ZENBIO,310063), anti-cdc2 (abcam,ab32094), anti-Caspase3/p17/p19 (Proteintech,19677-1-AP), anti-MMP9 (Proteintech,10375-2-AP), anti-E-cadherin (Proteintech,60335-1-Ig), anti-vimentin (Proteintech, 60330-1-Ig), anti-MEK1/2 (Proteintech, 11049-1-AP), anti-p-ERK1/2 (ZENBIO, 301245), anti-MEK1/2 (Proteintech, 11049-1-AP), anti-pMEK1/2 (Abcam, ab4750), anti-Raf (Abcam, ab200653), anti-p-Raf (Abcam, ab173539), and anti-βactin 1:1,000 (Cell Signaling Technology, 3700), goat anti-rabbit HRP-coupled secondary antibodies 1:5,000 (Proteintech, SA00001-2).

    Article Title: Targeting ZMYND8 unleashes IL-2 signalling to override T cell exhaustion.
    Article Snippet: Secondary antibodies and dilutions were as follows: HRP-conjugated anti-rabbit IgG (H + L) (1:3,000, W4011) and HRP-conjugated anti-mouse IgG (H + L) (1:3,000; W4021) were from Promega, and HRP-conjugated anti-rabbit IgG, light chain specific (1:3,000; SA00001-7L) was from Proteintech.

    Incubation:

    Article Title: DKK1/SMDT1 participate in Ang II-induced mitochondrial injury of human smooth muscle cells in an abdominal aortic aneurysm cell model
    Article Snippet: Next the membranes were blocked in 5% skimmed milk powder for 15 min at room temperature (RT), and then were incubated with primary antibodies at 4 ̊C overnight; the primary antibodies included anti-DKK1 (1:1,000; cat. no. ab109416; Abcam) and GAPDH (1:5,000; cat. no. 60004-1-Ig; ProteinTech Group, Inc.). .. Membranes were then incubated with secondary antibodies at RT for 2 h the next day and included HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L), (1:5,000; cat. no. SA00001-1; ProteinTech Group, Inc.) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (1:10,000; cat. no. ZB-2301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.). .. The bands were exposed using Tanon5200 (Tanon Science and Technology Co., Ltd.).

    Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
    Article Snippet: .. After washing, the membranes were incubated with secondary antibodies (HRP-conjugated goat anti-rabbit IgG) at room temperature for 2 h (1:10,000; cat. no. SA00001-2; Proteintech Group, Inc.). .. Protein detection was performed using an ultra-sensitive enhanced chemiluminescence kit (Shanghai Yamei Biomedical Technology Co Ltd.) and using ImageJ software (version 1.53k; National Institutes of Health).

    Virus:

    Article Title: UFMylation-dependent inhibition of AKT signaling by PHLDA3 in lung adenocarcinoma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies UFL1 antibody Bethyl Cat# A303-456A; RRID:AB_10951658 PHLDA3 antibody Abcam Cat# ab22822; RRID:AB_777241 His antibody Abcam Cat# ab9108; RRID:AB_307016 UFM1 antibody Abcam Cat# ab109305; RRID:AB_10864675 p-AKT (S473) antibody Cell Signaling Technology Cat# 4060; RRID:AB_2315049 p-AKT (T308) antibody Cell Signaling Technology Cat# 13038; RRID:AB_2629447 Cleaved-PARP1 antibody Cell Signaling Technology Cat# 5625; RRID:AB_10699459 AKT antibody Proteintech Cat# 60203-2-Ig; RRID:AB_10912803 p21 antibody Proteintech Cat# 10355-1-AP; RRID:AB_2077682 p53 antibody Proteintech Cat# 60283-2-Ig; RRID:AB_2881401 UFSP2 antibody Proteintech Cat# 16999-1-AP; RRID:AB_2214070 ATP1A1 antibody Bioswamp Cat# PAB30171; N/A Myc antibody Santa Cruz Biotechnology Cat# sc-40 AC; RRID:AB_2857941 Flag antibody Sigma-Aldrich Cat# F1804; RRID:AB_262044 HA antibody Sigma-Aldrich Cat# H3663; RRID:AB_262051 β-Actin antibody Sigma-Aldrich Cat# A1978; RRID:AB_476692 PRAS40 antibody Selleck Cat# F0239; N/A p-PRAS40 (T246) antibody Selleck Cat# F0252; N/A GSK3β antibody Selleck Cat# F0239; N/A p-GSK3β (S9) antibody Huabio Cat# ET1607-60; RRID:AB_3069776 Goat anti-mouse IgG (H + L), HRP conjugate Proteintech Cat# SA00001-1; RRID:AB_2722565 Goat anti-rabbit IgG (H + L), HRP conjugate Proteintech Cat# SA00001-2; RRID:AB_2722564 HRP-conjugated Affinipure Rabbit Anti-Goat IgG (H + L) Proteintech Cat# SA00001-4; RRID:AB_2864335 HRP Conjugated Goat Anti-Mouse IgG Light Chain Specific Abbkine Cat# A25012; RRID:AB_2737290 IPKine goat anti-rabbit IgG heavy chain secondary antibody, HRP-labeled (to eliminate light chain interference) Abbkine Cat# A25222; RRID:AB_2922982 Donkey Anti-Mouse IgG (H + L) Antibody, Alexa Fluor 488 Conjugated Thermo Fisher Scientific Cat# A-21202; RRID:AB_141607 Bacterial and virus strains Trelief 5α Chemically Competent Cell TsingkeBiotechnologyCo.,Ltd. ..

    Western Blot:

    Article Title: The regulatory role of circGDI2 in hepatocellular carcinoma proliferation and glycolysis with the involvement of m6A modification
    Article Snippet: β-actin , Western blot , Rabbit , 1:9000 , Proteintech , 20536-1-AP. .. HRP-conjugated Goat Anti-Rabbit IgG (H + L) , Western blot , Goat , 1:10000 , Proteintech , SA00001-2. .. Ki67 , IHC , Rabbit , 1:500 , Proteintech , 84432-1-RR.



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    The information on the antibody.

    Journal: Aging Cell

    Article Title: Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia

    doi: 10.1111/acel.70732

    Figure Lengend Snippet: The information on the antibody.

    Article Snippet: HRP goat anti‐mouse IgG , 1:5000 , SA00001‐1 , / , Proteintech , USA.

    Techniques: