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Chromogenix chromogenic substrates for plasmin (s2251)
Chromogenic Substrates For Plasmin (S2251), supplied by Chromogenix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s2251/chromogenic+substrate+s+2238/pm38735446-58-3-23
Average 90 stars, based on 1 article reviews
chromogenic substrates for plasmin (s2251) - by Bioz Stars, 2026-09
90/100 stars

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Concentration Assay:

Article Title: Increased Mosquito Midgut Infection by Dengue Virus Recruitment of Plasmin Is Blocked by an Endogenous Kazal-type Inhibitor
Article Snippet: .. Protease Concentration, nM Substrate (Supplier) Concentration, mM Plasmin 3.61 S2251 (Chromogenix) 1.2 Factor XIIa 20 S2302 (Chromogenix) 1 Factor XIa 0.125 S2366 (Chromogenix) 1 Factor Xa 0.43 S2765 (Chromogenix) 0.65 Factor IXa 333 FIXa (Spectrozyme) 0.4 Factor VIIa 460 S2288 (Chromogenix) 1 Kallikrein 0.93 S2302 (Chromogenix) 1.1 Alpha Thrombin 0.81 S2238 (Chromogenix) 0.1 Transparent Methods Mosquito colony and rearing An Aedes aegypti mosquito colony was established in 2010 from eggs collected in Singapore (Mendenhall et al. 2017). .. Larvae were reared in Milli‐Q water and fed with a mixture of liver powder (MP Biomedicals) and fish food (Tetramin Crisps Pro).

Article Title: Analysis of the thrombotic and fibrinolytic activities of tumor cell–derived extracellular vesicles
Article Snippet: .. The cells were washed with Hank’s balanced salt solution (Thermo Scientific), incubated with plasminogen (0.5 μM final concentration) for 10 minutes at 37°C, followed by addition of the chromogenic substrate S2251 (0.5 mM final concentration; Chromogenix; Diapharma), and plasmin generation was measured at 405 and 600 nm every 4 minutes for 4 hours at 37°C using a spectrophotometer (SpectraMax M3). .. Statistics Results were analyzed using GraphPad Prism 6.0.

Chromogenic Assay:

Article Title: Thrombolysis by chemically modified coagulation factor Xa
Article Snippet: Reagents and proteins Tetrasodium ethylenediaminetetraacetic acid (EDTA) and sodium chloride (Fisher Scientific, Nepean, ON, Canada), calcium chloride (EMD Chemicals, Inc., Gibbstown, NJ, USA), chromogenic substrate N-a-Benzyloxycarbonyl-D-Arg-Gly-Arg-pnitroaniline-dihydrochloride (S2765, DiaPharma, West Chester, OH, USA), HEPES and PEG 8000 were obtained from Sigma-Aldrich (Oakville, ON, Canada). .. Chromogenic assay At room temperature, the conversion of purified Lys-plasminogen (0.5 μM) to plasmin by tPA or urokinase was followed using the chromogenic substrate S2251 (Chromogenix-Diapharma, West Chester, OH, USA), as previously described [ 8 ]. .. Na-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CHCl 2 (ATA-FPRck) was purchased from Molecular Innovations (Novi, MI, USA). α-lysyl-([N-maleimidopropionamido]-tetraethylene glycol) ester (Lys-mTEG) was synthesized through an academic affiliation with the Centre for Drug Research and Development (Vancouver, BC, Canada).

Purification:

Article Title: Thrombolysis by chemically modified coagulation factor Xa
Article Snippet: Reagents and proteins Tetrasodium ethylenediaminetetraacetic acid (EDTA) and sodium chloride (Fisher Scientific, Nepean, ON, Canada), calcium chloride (EMD Chemicals, Inc., Gibbstown, NJ, USA), chromogenic substrate N-a-Benzyloxycarbonyl-D-Arg-Gly-Arg-pnitroaniline-dihydrochloride (S2765, DiaPharma, West Chester, OH, USA), HEPES and PEG 8000 were obtained from Sigma-Aldrich (Oakville, ON, Canada). .. Chromogenic assay At room temperature, the conversion of purified Lys-plasminogen (0.5 μM) to plasmin by tPA or urokinase was followed using the chromogenic substrate S2251 (Chromogenix-Diapharma, West Chester, OH, USA), as previously described [ 8 ]. .. Na-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CHCl 2 (ATA-FPRck) was purchased from Molecular Innovations (Novi, MI, USA). α-lysyl-([N-maleimidopropionamido]-tetraethylene glycol) ester (Lys-mTEG) was synthesized through an academic affiliation with the Centre for Drug Research and Development (Vancouver, BC, Canada).

Activation Assay:

Article Title: A Natural Inactivating Mutation in the CovS Component of the CovRS Regulatory Operon in a Pattern D Streptococcal pyogenes Strain Influences Virulence-associated Genes
Article Snippet: .. For hPg activation assays, 20 μl (∼1 × 10 7 cfu) of cells was added to individual wells of a 96-well Corning NBS nonbinding microwell plate, followed by 180 μl of 0.22 μ m hPg, 0.28 m m S2251 (H- d -Val-Leu-Lys- p -nitroanilide; Chromogenix, Milan, Italy) in the same buffer. ..

Incubation:

Article Title: Analysis of the thrombotic and fibrinolytic activities of tumor cell–derived extracellular vesicles
Article Snippet: .. The cells were washed with Hank’s balanced salt solution (Thermo Scientific), incubated with plasminogen (0.5 μM final concentration) for 10 minutes at 37°C, followed by addition of the chromogenic substrate S2251 (0.5 mM final concentration; Chromogenix; Diapharma), and plasmin generation was measured at 405 and 600 nm every 4 minutes for 4 hours at 37°C using a spectrophotometer (SpectraMax M3). .. Statistics Results were analyzed using GraphPad Prism 6.0.

Article Title: Thrombus-Targeted Theranostic Microbubbles: A New Technology towards Concurrent Rapid Ultrasound Diagnosis and Bleeding-free Fibrinolytic Treatment of Thrombosis
Article Snippet: .. 10nmol/L of scuPA was monitored against commercial uPA (0–15U/ml) of and were incubated with 400nmol/L of human glu-plasminogen (Sigma-Aldrich, Germany) and 1mmol/L of S2251 (Chromogenix, Italy). .. Samples were measured using the Bio-Rad Benchmark Plus (Bio-Rad, USA) at a wavelength of 405nm every 30 sec over a period of 60 min.

Article Title: Towards Effective and Safe Thrombolysis and Thromboprophylaxis
Article Snippet: .. Ten nmol/L of commercial uPA and scFv–scuPAs was incubated with 400 nmol/L of human glu-plasminogen (Sigma-Aldrich) and 1 mmol/L of S2251 (Chromogenix, Italy). .. Samples were measured using the Bio-Rad Benchmark Plus at a wavelength of 405 nm every 30 seconds over a period of 60 minutes.

Spectrophotometry:

Article Title: Analysis of the thrombotic and fibrinolytic activities of tumor cell–derived extracellular vesicles
Article Snippet: .. The cells were washed with Hank’s balanced salt solution (Thermo Scientific), incubated with plasminogen (0.5 μM final concentration) for 10 minutes at 37°C, followed by addition of the chromogenic substrate S2251 (0.5 mM final concentration; Chromogenix; Diapharma), and plasmin generation was measured at 405 and 600 nm every 4 minutes for 4 hours at 37°C using a spectrophotometer (SpectraMax M3). .. Statistics Results were analyzed using GraphPad Prism 6.0.

Clinical Proteomics:

Article Title: Fibrinogenolysis and fibrinolysis in vaccine-induced immune thrombocytopenia and thrombosis.
Article Snippet: Fibrinogen α-chain and FXIII-A subunit were detected using rabbit polyclonal antibodies (Abcam and Invitrogen respectively) and horseradish peroxidase-conjugated polyclonal swine anti-rabbit IgG (Dako). .. Plasmin generation in plasma (10%) was measured using 0.5 mM S2251 (Chromogenix) ± 10 nM tPA (Alteplase) and cyanogen bromide fibrinogen fragments (10 μg/ml; Technoclone). ..



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