focused ultrasonicator (Covaris)

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Covaris focused ultrasonicator
Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 4690 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s220/S220+Focused-ultrasonicator/bio_rxiv__64898__2026__03__28__714793-259-10-11
Average 99 stars, based on 4690 article reviews

focused ultrasonicator - by Bioz Stars, 2026-10

99/100 stars

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Sonication:

Article Title: Ex vivo long-term expansion of human hematopoietic stem and progenitor cells as a tool for modeling vector integration sites and clonality
Article Snippet: Thus, genomic DNA was isolated from HSPCs using the DNeasy Blood & Tissue Kit (Qiagen). .. DNA was sheared by sonication using the S220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). .. Purification was performed with AMPure Beads (Beckman and Coulter) in a 0.7-fold ratio.

Lysis:

Article Title: Regulation of PKM2 expression and function by GLIS3 during metabolic reprogramming in polycystic kidneys.
Article Snippet: .. Samples were subsequently sheared in lysis buffer C for 40min using an S220 focused-ultrasonicator (Covaris). .. After centrifugation, the cleared chromatin supernatant was incubated with an anti-GFP (ab290; Abcam) or anti-HNF-1B (720259; ThermoFisher) antibody for ChIP.

Article Title: Regulation of PKM2 expression and function by GLIS3 during metabolic reprogramming in polycystic kidneys
Article Snippet: .. Samples were subsequently sheared in lysis buffer C for 40 min using an S220 focused-ultrasonicator (Covaris). .. After centrifugation, the cleared chromatin supernatant was incubated with an anti-GFP (ab290; Abcam) or anti-HNF-1B (720259; ThermoFisher) antibody for ChIP.

Size Selection:

Article Title: Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy
Article Snippet: .. Preparation steps briefly: DNA shearing into 420 bp fragments by S220 focused-ultrasonicator (Covaris) followed by size selection using magnetic beads; end repair and A-tailing; adapter ligation followed by magnetic beads cleanup of adapter-ligated DNA; PCR amplification and cleanup of the product; quality control; denaturation; single strand circularization; enzymatic digestion; cleanup of enzymatic digestion product; quality control. .. The quality and quantity of the resulting libraries was determined with an Agilent Bioanalyzer 2100 and a Qubit® 2.0 fluorometer.

Article Title: Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy.
Article Snippet: .. Preparation steps briefly: DNA shearing into 420 bp fragments by S220 focused-ultrasonicator (Covaris) followed by size selection using magnetic beads; end repair and A-tailing; adapter ligation followed by magnetic beads cleanup of adapter-ligated DNA; PCR amplification and cleanup of the product; quality control; denaturation; single strand circularization; enzymatic digestion; cleanup of enzymatic digestion product; quality control. .. The quality and quantity of the resulting libraries was determined with an Agilent Bioanalyzer 2100 and a Qubit® 2.0 fluorometer.

Magnetic Beads:

Article Title: Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy.
Article Snippet: .. Preparation steps briefly: DNA shearing into 420 bp fragments by S220 focused-ultrasonicator (Covaris) followed by size selection using magnetic beads; end repair and A-tailing; adapter ligation followed by magnetic beads cleanup of adapter-ligated DNA; PCR amplification and cleanup of the product; quality control; denaturation; single strand circularization; enzymatic digestion; cleanup of enzymatic digestion product; quality control. .. The quality and quantity of the resulting libraries was determined with an Agilent Bioanalyzer 2100 and a Qubit® 2.0 fluorometer.

Adapter Ligation:

Article Title: Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy.
Article Snippet: .. Preparation steps briefly: DNA shearing into 420 bp fragments by S220 focused-ultrasonicator (Covaris) followed by size selection using magnetic beads; end repair and A-tailing; adapter ligation followed by magnetic beads cleanup of adapter-ligated DNA; PCR amplification and cleanup of the product; quality control; denaturation; single strand circularization; enzymatic digestion; cleanup of enzymatic digestion product; quality control. .. The quality and quantity of the resulting libraries was determined with an Agilent Bioanalyzer 2100 and a Qubit® 2.0 fluorometer.

Polymerase Chain Reaction:

Article Title: Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy.
Article Snippet: .. Preparation steps briefly: DNA shearing into 420 bp fragments by S220 focused-ultrasonicator (Covaris) followed by size selection using magnetic beads; end repair and A-tailing; adapter ligation followed by magnetic beads cleanup of adapter-ligated DNA; PCR amplification and cleanup of the product; quality control; denaturation; single strand circularization; enzymatic digestion; cleanup of enzymatic digestion product; quality control. .. The quality and quantity of the resulting libraries was determined with an Agilent Bioanalyzer 2100 and a Qubit® 2.0 fluorometer.

Amplification:

Article Title: Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy.
Article Snippet: .. Preparation steps briefly: DNA shearing into 420 bp fragments by S220 focused-ultrasonicator (Covaris) followed by size selection using magnetic beads; end repair and A-tailing; adapter ligation followed by magnetic beads cleanup of adapter-ligated DNA; PCR amplification and cleanup of the product; quality control; denaturation; single strand circularization; enzymatic digestion; cleanup of enzymatic digestion product; quality control. .. The quality and quantity of the resulting libraries was determined with an Agilent Bioanalyzer 2100 and a Qubit® 2.0 fluorometer.

Control:

Article Title: Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy.
Article Snippet: .. Preparation steps briefly: DNA shearing into 420 bp fragments by S220 focused-ultrasonicator (Covaris) followed by size selection using magnetic beads; end repair and A-tailing; adapter ligation followed by magnetic beads cleanup of adapter-ligated DNA; PCR amplification and cleanup of the product; quality control; denaturation; single strand circularization; enzymatic digestion; cleanup of enzymatic digestion product; quality control. .. The quality and quantity of the resulting libraries was determined with an Agilent Bioanalyzer 2100 and a Qubit® 2.0 fluorometer.

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