Review



cgrp  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Proteintech cgrp
    NM-LP TK /RSV-MnCDs alleviates discogenic pain in IVDD. (A) Experimental timeline schematic. (B–C) Open-field behavioral analysis showing locomotor activity parameters (active time, central mean speed, total distance, and mean speed) across groups (n = 5). (D–E) PWL and PWT test showing alleviation of thermal and mechanical allodynia by NM-LP TK /RSV-MnCDs in LSI mice (n = 5). (F–G) IHC staining and quantification analyses of DRG sections showing expression of sensory neuropeptides <t>CGRP</t> and SP (n = 5). Scale bar, 100 μm. (H–I) IF co-labeling <t>of</t> <t>PGP9.5</t> and CGRP/SP in DRG, and corresponding quantification analyses (n = 5). Scale bar, 100 μm. Data are presented as mean ± SD. For comparisons among multiple groups at a single time point, one-way ANOVA followed was employed to determine statistical significance. For datasets involving multiple groups evaluated over time, including PWL and PWT assessments, a two-way repeated measures ANOVA was utilized. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns = not significant.
    Cgrp, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s100a12/S100A12+Antibody/pmc12723136-149-85-84
    Average 94 stars, based on 19 article reviews
    cgrp - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy"

    Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.11.048

    NM-LP TK /RSV-MnCDs alleviates discogenic pain in IVDD. (A) Experimental timeline schematic. (B–C) Open-field behavioral analysis showing locomotor activity parameters (active time, central mean speed, total distance, and mean speed) across groups (n = 5). (D–E) PWL and PWT test showing alleviation of thermal and mechanical allodynia by NM-LP TK /RSV-MnCDs in LSI mice (n = 5). (F–G) IHC staining and quantification analyses of DRG sections showing expression of sensory neuropeptides CGRP and SP (n = 5). Scale bar, 100 μm. (H–I) IF co-labeling of PGP9.5 and CGRP/SP in DRG, and corresponding quantification analyses (n = 5). Scale bar, 100 μm. Data are presented as mean ± SD. For comparisons among multiple groups at a single time point, one-way ANOVA followed was employed to determine statistical significance. For datasets involving multiple groups evaluated over time, including PWL and PWT assessments, a two-way repeated measures ANOVA was utilized. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns = not significant.
    Figure Legend Snippet: NM-LP TK /RSV-MnCDs alleviates discogenic pain in IVDD. (A) Experimental timeline schematic. (B–C) Open-field behavioral analysis showing locomotor activity parameters (active time, central mean speed, total distance, and mean speed) across groups (n = 5). (D–E) PWL and PWT test showing alleviation of thermal and mechanical allodynia by NM-LP TK /RSV-MnCDs in LSI mice (n = 5). (F–G) IHC staining and quantification analyses of DRG sections showing expression of sensory neuropeptides CGRP and SP (n = 5). Scale bar, 100 μm. (H–I) IF co-labeling of PGP9.5 and CGRP/SP in DRG, and corresponding quantification analyses (n = 5). Scale bar, 100 μm. Data are presented as mean ± SD. For comparisons among multiple groups at a single time point, one-way ANOVA followed was employed to determine statistical significance. For datasets involving multiple groups evaluated over time, including PWL and PWT assessments, a two-way repeated measures ANOVA was utilized. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns = not significant.

    Techniques Used: Activity Assay, Immunohistochemistry, Expressing, Labeling

    Related Articles

    Marker:

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis
    Article Snippet: .. Primary antibodies against CD68 (macrophage marker; 25747-1-AP, Proteintech; 1:200 dilution) and S100A12 (inflammatory marker; 16630-1-AP, Proteintech; 1:100 dilution) were applied overnight at 4 °C. .. After being washed with TBS-T (0.1% Tween-20 in TBS), the sections were incubated with species-matched horseradish peroxidase (HRP)-conjugated secondary antibodies (CW2069S, CWBIO) for 45 min at RT.

    Membrane:

    Article Title: Comparative Study on the Sperm Proteomes of Horses and Donkeys
    Article Snippet: Horse and donkey sperm were lysed in 2× loading buffer (Thermo Fisher Scientific, Waltham, MA, USA), the proteins were separated on a 12% polyacrylamide gel, and then the proteins were transferred to a nitrocellulose membrane. .. The membrane was blocked in nonfat milk powder for 1 h at 37 °C and then incubated with antibodies against AKAP4 (1:500; 24986-1-AP, Proteintech, Wuhan, China), CABYR (1:500; 12351-1-AP, Proteintech), ACADSB (1:500; 13122-1-AP, Proteintech), S100A12 (1:500; 16630-1-AP, Proteintech), and tubulin (1:1000; ab6046, Abcam, Cambridge, MA, USA) overnight at 4 °C. .. After washing with TBST, the membrane was incubated with a secondary antibody at room temperature for 1.5 h. The protein bands were visualized with enhanced chemiluminescence (ECL) Plus (GEL, San Francisco, CA, USA) and visualized with a Gel Doc XR+ Gel Documentation System (Bio-Rad, Hercules, CA, USA).

    Incubation:

    Article Title: Comparative Study on the Sperm Proteomes of Horses and Donkeys
    Article Snippet: Horse and donkey sperm were lysed in 2× loading buffer (Thermo Fisher Scientific, Waltham, MA, USA), the proteins were separated on a 12% polyacrylamide gel, and then the proteins were transferred to a nitrocellulose membrane. .. The membrane was blocked in nonfat milk powder for 1 h at 37 °C and then incubated with antibodies against AKAP4 (1:500; 24986-1-AP, Proteintech, Wuhan, China), CABYR (1:500; 12351-1-AP, Proteintech), ACADSB (1:500; 13122-1-AP, Proteintech), S100A12 (1:500; 16630-1-AP, Proteintech), and tubulin (1:1000; ab6046, Abcam, Cambridge, MA, USA) overnight at 4 °C. .. After washing with TBST, the membrane was incubated with a secondary antibody at room temperature for 1.5 h. The protein bands were visualized with enhanced chemiluminescence (ECL) Plus (GEL, San Francisco, CA, USA) and visualized with a Gel Doc XR+ Gel Documentation System (Bio-Rad, Hercules, CA, USA).

    Article Title: Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling†.
    Article Snippet: Given recent reports of expression of postnatal mineral transport regulators at the maternal–conceptus interface during the peri-implantation period, this study tested the hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, and vitamin D signaling in the ovine endometrium.. Mature Rambouillet ewes (n = 24) were surgically fitted with intrauterine catheters on day 7 of the estrous cycle.. Ewes received daily intramuscular injections of 50 mg of P4 in corn oil vehicle and 75 mg of progesterone receptor antagonist (RU486) in corn oil from days 8 to 15, and twice-daily intrauterine injections of either control proteins (CX) or IFNT (25 μg/uterine horn/day) from days 11 to 15 resulting in four treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT.

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis.
    Article Snippet: .. Double immunofluorescence staining was 254 performed as follows: sections were incubated overnight at 4°C 255 with primary antibodies against CD68 (mouse monoclonal, 1:100 256 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit 257 polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 258 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 259 dilution; 22392-1-AP, Proteintech, China). .. After being washed with 260 PBS, the sections were incubated with secondary antibodies: Alexa 261 Fluor 488-conjugated goat anti-mouse IgG (1:500 dilution; A-11001, 262 Thermo Fisher Scientific, USA) with Alexa Fluor 594-conjugated 263 goat anti-rabbit IgG (1:500 dilution; A-11012, Thermo Fisher 264 Scientific) for 1 hour at room temperature in the dark.

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis
    Article Snippet: .. Double immunofluorescence staining was performed as follows: sections were incubated overnight at 4 °C with primary antibodies against CD68 (mouse monoclonal, 1:100 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 dilution; 22392-1-AP, Proteintech, China). .. After being washed with PBS, the sections were incubated with secondary antibodies: Alexa Fluor 488-conjugated goat anti-mouse IgG (1:500 dilution; A-11001, Thermo Fisher Scientific, USA) with Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:500 dilution; A-11012, Thermo Fisher Scientific) for 1 h at room temperature in the dark.

    Protein Concentration:

    Article Title: Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling†.
    Article Snippet: Given recent reports of expression of postnatal mineral transport regulators at the maternal–conceptus interface during the peri-implantation period, this study tested the hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, and vitamin D signaling in the ovine endometrium.. Mature Rambouillet ewes (n = 24) were surgically fitted with intrauterine catheters on day 7 of the estrous cycle.. Ewes received daily intramuscular injections of 50 mg of P4 in corn oil vehicle and 75 mg of progesterone receptor antagonist (RU486) in corn oil from days 8 to 15, and twice-daily intrauterine injections of either control proteins (CX) or IFNT (25 μg/uterine horn/day) from days 11 to 15 resulting in four treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT.

    Negative Control:

    Article Title: Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling†.
    Article Snippet: Given recent reports of expression of postnatal mineral transport regulators at the maternal–conceptus interface during the peri-implantation period, this study tested the hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, and vitamin D signaling in the ovine endometrium.. Mature Rambouillet ewes (n = 24) were surgically fitted with intrauterine catheters on day 7 of the estrous cycle.. Ewes received daily intramuscular injections of 50 mg of P4 in corn oil vehicle and 75 mg of progesterone receptor antagonist (RU486) in corn oil from days 8 to 15, and twice-daily intrauterine injections of either control proteins (CX) or IFNT (25 μg/uterine horn/day) from days 11 to 15 resulting in four treatment groups: P4 + CX; P4 + IFNT; RU486 + P4 + CX; and RU486 + P4 + IFNT.

    Immunohistochemistry:

    Article Title: Single cell immunoprofile of synovial fluid in rheumatoid arthritis with TNF/JAK inhibitor treatment.
    Article Snippet: .. For co-colocalization of SPP1+ and CD36+CD62L-macrophages (CD68+), as well as S100A12+ and CD62L+macrophages (CD68+) in the ST from RA patient, all antibodies against CD68 (AF20022, Aifang Biological, 1:3000, TYR-520), SPP1(AF03532, Aifang Biological, 1:2000, TYR-570), CD36 (66395-1-Ig, Proteintech, 1:3000, TYR-780), CD62L (222511, ZEN-BIOSCIENCE, 1:2000, TYR-690), and S100A12 (14959, Cell Signaling Technology, 1:2000, TYR-620) were evaluated via immunohistochemistry. .. For co-colocalization of SPP1+ macrophages and CXCL13+CD4+ T cell in the ST from RA and OA patients, all antibodies against CD68 (AF20022, Aifang Biological, 1:3000, TYR-690), SPP1(AF03532, Aifang Biological, 1:2000, TYR-620), CD3 (85061S, Cell Signaling Technology, 1:200, TYR-780), CD4 (ab133616, Abcam, 1:400, TYR480), CXCL13 (ab246518, Abcam, 1:500, TYR-570) and STAT1 (14994T, Cell Signaling Technology, 1:1000, TYR-520) were evaluated via immunohistochemistry.

    Article Title: Single cell immunoprofile of synovial fluid in rheumatoid arthritis with TNF/JAK inhibitor treatment
    Article Snippet: .. For co-colocalization of SPP1 + and CD36 + CD62L - macrophages (CD68 + ), as well as S100A12 + and CD62L + macrophages (CD68 + ) in the ST from RA patient, all antibodies against CD68 (AF20022, Aifang Biological, 1:3000, TYR-520), SPP1(AF03532, Aifang Biological, 1:2000, TYR-570), CD36 (66395-1-Ig, Proteintech, 1:3000, TYR-780), CD62L (222511, ZEN-BIOSCIENCE, 1:2000, TYR-690), and S100A12 (14959, Cell Signaling Technology, 1:2000, TYR-620) were evaluated via immunohistochemistry. .. For co-colocalization of SPP1 + macrophages and CXCL13 + CD4 + T cell in the ST from RA and OA patients, all antibodies against CD68 (AF20022, Aifang Biological, 1:3000, TYR-690), SPP1(AF03532, Aifang Biological, 1:2000, TYR-620), CD3 (85061S, Cell Signaling Technology, 1:200, TYR-780), CD4 (ab133616, Abcam, 1:400, TYR-480), CXCL13 (ab246518, Abcam, 1:500, TYR-570) and STAT1 (14994T, Cell Signaling Technology, 1:1000, TYR-520) were evaluated via immunohistochemistry.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Clinical features and nasal inflammation in asthma and allergic rhinitis.
    Article Snippet: The levels of IL-4, IL-13, IL-25, S100A8, S100A9, and S100A12 were analyzed by CUSABIO ELISA kits (CUSABIO, Wuhan, China). .. Blood samples of IL-4, IL-5, IL-25, and S100A12 were measured by standard quantitative ELISA kits (MultiSciences, Hangzhou, China; ElabScience, Wuhan, China; Proteintech, Wuhan, China; Abcam, Cambridge, UK). .. The lowest detection limit for each cytokine and chemokine were set as follows: R&D Systems: IL-5, 3.9 pg/ml; IL-33, 3.13 pg/ml; IL-17, 31.3 pg/ml.

    Double Immunofluorescence Staining:

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis.
    Article Snippet: .. Double immunofluorescence staining was 254 performed as follows: sections were incubated overnight at 4°C 255 with primary antibodies against CD68 (mouse monoclonal, 1:100 256 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit 257 polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 258 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 259 dilution; 22392-1-AP, Proteintech, China). .. After being washed with 260 PBS, the sections were incubated with secondary antibodies: Alexa 261 Fluor 488-conjugated goat anti-mouse IgG (1:500 dilution; A-11001, 262 Thermo Fisher Scientific, USA) with Alexa Fluor 594-conjugated 263 goat anti-rabbit IgG (1:500 dilution; A-11012, Thermo Fisher 264 Scientific) for 1 hour at room temperature in the dark.

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis
    Article Snippet: .. Double immunofluorescence staining was performed as follows: sections were incubated overnight at 4 °C with primary antibodies against CD68 (mouse monoclonal, 1:100 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 dilution; 22392-1-AP, Proteintech, China). .. After being washed with PBS, the sections were incubated with secondary antibodies: Alexa Fluor 488-conjugated goat anti-mouse IgG (1:500 dilution; A-11001, Thermo Fisher Scientific, USA) with Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:500 dilution; A-11012, Thermo Fisher Scientific) for 1 h at room temperature in the dark.



    Similar Products

    cgrp  (Proteintech)
    94
    Proteintech cgrp
    NM-LP TK /RSV-MnCDs alleviates discogenic pain in IVDD. (A) Experimental timeline schematic. (B–C) Open-field behavioral analysis showing locomotor activity parameters (active time, central mean speed, total distance, and mean speed) across groups (n = 5). (D–E) PWL and PWT test showing alleviation of thermal and mechanical allodynia by NM-LP TK /RSV-MnCDs in LSI mice (n = 5). (F–G) IHC staining and quantification analyses of DRG sections showing expression of sensory neuropeptides <t>CGRP</t> and SP (n = 5). Scale bar, 100 μm. (H–I) IF co-labeling <t>of</t> <t>PGP9.5</t> and CGRP/SP in DRG, and corresponding quantification analyses (n = 5). Scale bar, 100 μm. Data are presented as mean ± SD. For comparisons among multiple groups at a single time point, one-way ANOVA followed was employed to determine statistical significance. For datasets involving multiple groups evaluated over time, including PWL and PWT assessments, a two-way repeated measures ANOVA was utilized. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns = not significant.
    Cgrp, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s100a12/S100A12+Antibody/pmc12723136-149-85-84
    Average 94 stars, based on 1 article reviews
    cgrp - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    s100a12  (Human Protein Atlas)
    86
    Human Protein Atlas s100a12
    NM-LP TK /RSV-MnCDs alleviates discogenic pain in IVDD. (A) Experimental timeline schematic. (B–C) Open-field behavioral analysis showing locomotor activity parameters (active time, central mean speed, total distance, and mean speed) across groups (n = 5). (D–E) PWL and PWT test showing alleviation of thermal and mechanical allodynia by NM-LP TK /RSV-MnCDs in LSI mice (n = 5). (F–G) IHC staining and quantification analyses of DRG sections showing expression of sensory neuropeptides <t>CGRP</t> and SP (n = 5). Scale bar, 100 μm. (H–I) IF co-labeling <t>of</t> <t>PGP9.5</t> and CGRP/SP in DRG, and corresponding quantification analyses (n = 5). Scale bar, 100 μm. Data are presented as mean ± SD. For comparisons among multiple groups at a single time point, one-way ANOVA followed was employed to determine statistical significance. For datasets involving multiple groups evaluated over time, including PWL and PWT assessments, a two-way repeated measures ANOVA was utilized. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns = not significant.
    S100a12, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s100a12/s100a12/10__3390_slash_ijms27031243-121-6-2
    Average 86 stars, based on 1 article reviews
    s100a12 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    s100a12  (Proteintech)
    94
    Proteintech s100a12
    Increased frequency and proinflammatory signature of CD48 high <t>S100A12</t> + macrophages in rheumatoid arthritis synovium. A UMAP visualization of synovial macrophages clustered into nine subpopulations on the basis of scRNA-seq data. B Disease-stratified analysis showing an increased proportion of CD48 high S100A12 + macrophages in the RA synovium compared with those in the UA, OA, and HC groups. C UMAP plots depicting S100A12 expression intensity across macrophage subclusters in different disease states. D Gene Ontology (GO) enrichment of biological processes in CD48 high S100A12 + marker genes, highlighting enrichment for defense response activation, cytokine production, and leukocyte migration. E Representative images of CD68 and S100A12 immunofluorescence staining in knee synovial tissues from RA and OA patients. Scale bar: 100 μm
    S100a12, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s100a12/S100A12+Antibody/pmc12801948-76-27-33
    Average 94 stars, based on 1 article reviews
    s100a12 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    rabbit anti s100a12 antibody  (Proteintech)
    94
    Proteintech rabbit anti s100a12 antibody
    Increased frequency and proinflammatory signature of CD48 high <t>S100A12</t> + macrophages in rheumatoid arthritis synovium. A UMAP visualization of synovial macrophages clustered into nine subpopulations on the basis of scRNA-seq data. B Disease-stratified analysis showing an increased proportion of CD48 high S100A12 + macrophages in the RA synovium compared with those in the UA, OA, and HC groups. C UMAP plots depicting S100A12 expression intensity across macrophage subclusters in different disease states. D Gene Ontology (GO) enrichment of biological processes in CD48 high S100A12 + marker genes, highlighting enrichment for defense response activation, cytokine production, and leukocyte migration. E Representative images of CD68 and S100A12 immunofluorescence staining in knee synovial tissues from RA and OA patients. Scale bar: 100 μm
    Rabbit Anti S100a12 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s100a12/S100A12+Antibody/pm41361849-196-41-46
    Average 94 stars, based on 1 article reviews
    rabbit anti s100a12 antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    s100a12 276  (Proteintech)
    94
    Proteintech s100a12 276
    Increased frequency and proinflammatory signature of CD48 high <t>S100A12</t> + macrophages in rheumatoid arthritis synovium. A UMAP visualization of synovial macrophages clustered into nine subpopulations on the basis of scRNA-seq data. B Disease-stratified analysis showing an increased proportion of CD48 high S100A12 + macrophages in the RA synovium compared with those in the UA, OA, and HC groups. C UMAP plots depicting S100A12 expression intensity across macrophage subclusters in different disease states. D Gene Ontology (GO) enrichment of biological processes in CD48 high S100A12 + marker genes, highlighting enrichment for defense response activation, cytokine production, and leukocyte migration. E Representative images of CD68 and S100A12 immunofluorescence staining in knee synovial tissues from RA and OA patients. Scale bar: 100 μm
    S100a12 276, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s100a12/S100A12+Antibody/pm41361849-115-12-17
    Average 94 stars, based on 1 article reviews
    s100a12 276 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti s100a12 antibody  (Proteintech)
    94
    Proteintech rabbit polyclonal anti s100a12 antibody
    Increased frequency and proinflammatory signature of CD48 high <t>S100A12</t> + macrophages in rheumatoid arthritis synovium. A UMAP visualization of synovial macrophages clustered into nine subpopulations on the basis of scRNA-seq data. B Disease-stratified analysis showing an increased proportion of CD48 high S100A12 + macrophages in the RA synovium compared with those in the UA, OA, and HC groups. C UMAP plots depicting S100A12 expression intensity across macrophage subclusters in different disease states. D Gene Ontology (GO) enrichment of biological processes in CD48 high S100A12 + marker genes, highlighting enrichment for defense response activation, cytokine production, and leukocyte migration. E Representative images of CD68 and S100A12 immunofluorescence staining in knee synovial tissues from RA and OA patients. Scale bar: 100 μm
    Rabbit Polyclonal Anti S100a12 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s100a12/S100A12+Antibody/pm41361849-191-50-58
    Average 94 stars, based on 1 article reviews
    rabbit polyclonal anti s100a12 antibody - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    NM-LP TK /RSV-MnCDs alleviates discogenic pain in IVDD. (A) Experimental timeline schematic. (B–C) Open-field behavioral analysis showing locomotor activity parameters (active time, central mean speed, total distance, and mean speed) across groups (n = 5). (D–E) PWL and PWT test showing alleviation of thermal and mechanical allodynia by NM-LP TK /RSV-MnCDs in LSI mice (n = 5). (F–G) IHC staining and quantification analyses of DRG sections showing expression of sensory neuropeptides CGRP and SP (n = 5). Scale bar, 100 μm. (H–I) IF co-labeling of PGP9.5 and CGRP/SP in DRG, and corresponding quantification analyses (n = 5). Scale bar, 100 μm. Data are presented as mean ± SD. For comparisons among multiple groups at a single time point, one-way ANOVA followed was employed to determine statistical significance. For datasets involving multiple groups evaluated over time, including PWL and PWT assessments, a two-way repeated measures ANOVA was utilized. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns = not significant.

    Journal: Bioactive Materials

    Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy

    doi: 10.1016/j.bioactmat.2025.11.048

    Figure Lengend Snippet: NM-LP TK /RSV-MnCDs alleviates discogenic pain in IVDD. (A) Experimental timeline schematic. (B–C) Open-field behavioral analysis showing locomotor activity parameters (active time, central mean speed, total distance, and mean speed) across groups (n = 5). (D–E) PWL and PWT test showing alleviation of thermal and mechanical allodynia by NM-LP TK /RSV-MnCDs in LSI mice (n = 5). (F–G) IHC staining and quantification analyses of DRG sections showing expression of sensory neuropeptides CGRP and SP (n = 5). Scale bar, 100 μm. (H–I) IF co-labeling of PGP9.5 and CGRP/SP in DRG, and corresponding quantification analyses (n = 5). Scale bar, 100 μm. Data are presented as mean ± SD. For comparisons among multiple groups at a single time point, one-way ANOVA followed was employed to determine statistical significance. For datasets involving multiple groups evaluated over time, including PWL and PWT assessments, a two-way repeated measures ANOVA was utilized. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001; ns = not significant.

    Article Snippet: For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and counterstaining with DAPI (Beyotime, Cat. No. C1006).

    Techniques: Activity Assay, Immunohistochemistry, Expressing, Labeling

    Increased frequency and proinflammatory signature of CD48 high S100A12 + macrophages in rheumatoid arthritis synovium. A UMAP visualization of synovial macrophages clustered into nine subpopulations on the basis of scRNA-seq data. B Disease-stratified analysis showing an increased proportion of CD48 high S100A12 + macrophages in the RA synovium compared with those in the UA, OA, and HC groups. C UMAP plots depicting S100A12 expression intensity across macrophage subclusters in different disease states. D Gene Ontology (GO) enrichment of biological processes in CD48 high S100A12 + marker genes, highlighting enrichment for defense response activation, cytokine production, and leukocyte migration. E Representative images of CD68 and S100A12 immunofluorescence staining in knee synovial tissues from RA and OA patients. Scale bar: 100 μm

    Journal: Arthritis Research & Therapy

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis

    doi: 10.1186/s13075-025-03708-3

    Figure Lengend Snippet: Increased frequency and proinflammatory signature of CD48 high S100A12 + macrophages in rheumatoid arthritis synovium. A UMAP visualization of synovial macrophages clustered into nine subpopulations on the basis of scRNA-seq data. B Disease-stratified analysis showing an increased proportion of CD48 high S100A12 + macrophages in the RA synovium compared with those in the UA, OA, and HC groups. C UMAP plots depicting S100A12 expression intensity across macrophage subclusters in different disease states. D Gene Ontology (GO) enrichment of biological processes in CD48 high S100A12 + marker genes, highlighting enrichment for defense response activation, cytokine production, and leukocyte migration. E Representative images of CD68 and S100A12 immunofluorescence staining in knee synovial tissues from RA and OA patients. Scale bar: 100 μm

    Article Snippet: Double immunofluorescence staining was performed as follows: sections were incubated overnight at 4 °C with primary antibodies against CD68 (mouse monoclonal, 1:100 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 dilution; 22392-1-AP, Proteintech, China).

    Techniques: Expressing, Marker, Activation Assay, Migration, Immunofluorescence, Staining

    IRF7 is a specific transcriptional regulator of CD48 high S100A12 + macrophages. A Venn diagram showing overlapping transcription factors (TFs) identified by triplicate SCENIC analyses, with the CD48 high S100A12 + subcluster enriched for NFIL3, TGIF1, FOSL2, IRF7, and STAT1. B Heatmap of regulon activity scores (RASs) for TFs across macrophage subclusters. C Ranking of TFs in CD48 high S100A12 + macrophages by the regulon specificity score (RSS, calculated via Jensen‒Shannon divergence). D UMAP dimensionality reduction of TF activity profiles across subclusters. E – F UMAP plots highlighting spatial overlap between the CD48 high S100A12 + subcluster. ( E ) and cells with elevated IRF7 regulon activity ( F ). G Representative images of immunofluorescence staining for CD68 and IRF7 in knee synovial tissues from RA and OA patients. Scale bar: 100 μm

    Journal: Arthritis Research & Therapy

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis

    doi: 10.1186/s13075-025-03708-3

    Figure Lengend Snippet: IRF7 is a specific transcriptional regulator of CD48 high S100A12 + macrophages. A Venn diagram showing overlapping transcription factors (TFs) identified by triplicate SCENIC analyses, with the CD48 high S100A12 + subcluster enriched for NFIL3, TGIF1, FOSL2, IRF7, and STAT1. B Heatmap of regulon activity scores (RASs) for TFs across macrophage subclusters. C Ranking of TFs in CD48 high S100A12 + macrophages by the regulon specificity score (RSS, calculated via Jensen‒Shannon divergence). D UMAP dimensionality reduction of TF activity profiles across subclusters. E – F UMAP plots highlighting spatial overlap between the CD48 high S100A12 + subcluster. ( E ) and cells with elevated IRF7 regulon activity ( F ). G Representative images of immunofluorescence staining for CD68 and IRF7 in knee synovial tissues from RA and OA patients. Scale bar: 100 μm

    Article Snippet: Double immunofluorescence staining was performed as follows: sections were incubated overnight at 4 °C with primary antibodies against CD68 (mouse monoclonal, 1:100 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 dilution; 22392-1-AP, Proteintech, China).

    Techniques: Activity Assay, Immunofluorescence, Staining

    IRF7 directly regulates downstream inflammatory genes in M1 macrophages. A ChIP-seq peak heatmaps showing increased IRF7 binding to promoter/enhancer regions in LPS-stimulated M1 macrophages. B Venn diagram of 108 overlapping genes from the IRF7 ChIP-seq data and the SCENIC-predicted target genes. C Reactome pathway enrichment of IRF7-regulated genes, highlighting the involvement of NF-κB, TNF, and Toll-like receptor signalling (key genes: IL-1β, FOS, NF-κB1, PTGS2, and CXCL10). D Bulk RNA-seq heatmap showing the upregulation of IRF7 and target genes in M1-polarized macrophages ( GSE130011 , GSE154346 ). E UMAP plots of NFKB1, PTGS2, IL1B, and CXCL10 expression in the CD48 high S100A12 + subcluster. F RT‒qPCR analysis of IRF7 and M1 marker genes in siRNA-treated macrophages (performed in triplicate, with 3 distinct patient sources used for each repetition). G – H Western blot validation of IRF7 and downstream protein expression following IRF7 knockdown in M1-polarized macrophages (performed in triplicate, with 3 distinct patient sources used for each repetition). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way ANOVA with the Bonferroni post hoc correction)

    Journal: Arthritis Research & Therapy

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis

    doi: 10.1186/s13075-025-03708-3

    Figure Lengend Snippet: IRF7 directly regulates downstream inflammatory genes in M1 macrophages. A ChIP-seq peak heatmaps showing increased IRF7 binding to promoter/enhancer regions in LPS-stimulated M1 macrophages. B Venn diagram of 108 overlapping genes from the IRF7 ChIP-seq data and the SCENIC-predicted target genes. C Reactome pathway enrichment of IRF7-regulated genes, highlighting the involvement of NF-κB, TNF, and Toll-like receptor signalling (key genes: IL-1β, FOS, NF-κB1, PTGS2, and CXCL10). D Bulk RNA-seq heatmap showing the upregulation of IRF7 and target genes in M1-polarized macrophages ( GSE130011 , GSE154346 ). E UMAP plots of NFKB1, PTGS2, IL1B, and CXCL10 expression in the CD48 high S100A12 + subcluster. F RT‒qPCR analysis of IRF7 and M1 marker genes in siRNA-treated macrophages (performed in triplicate, with 3 distinct patient sources used for each repetition). G – H Western blot validation of IRF7 and downstream protein expression following IRF7 knockdown in M1-polarized macrophages (performed in triplicate, with 3 distinct patient sources used for each repetition). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way ANOVA with the Bonferroni post hoc correction)

    Article Snippet: Double immunofluorescence staining was performed as follows: sections were incubated overnight at 4 °C with primary antibodies against CD68 (mouse monoclonal, 1:100 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 dilution; 22392-1-AP, Proteintech, China).

    Techniques: ChIP-sequencing, Binding Assay, RNA Sequencing, Expressing, Marker, Western Blot, Biomarker Discovery, Knockdown

    Local IRF7 knockdown alters the immune cell composition in CIA mice. A Schematic of intra-articular IRF7 siRNA treatment in collagen-induced arthritis (CIA) model mice. B – C Flow cytometry analysis of the CD86 and CD206 mean fluorescence intensities (MFIs) in F4/80 + macrophages from ankle joints ( n = 6). CD86: NC: 1241 ± 265.4, si-IRF7: 2469 ± 390.3, positive: 3489 ± 570.9, si-mock: 3689 ± 370.1. CD206: NC: 2225 ± 225.9, si-IRF7: 3395 ± 369.4, positive: 978.2 ± 147.9, si-mock: 2022 ± 170.6. D – E Frequencies of Foxp3 + Tregs among CD3 + CD4 + T cells ( n = 6). NC: 1.66% ± 0.15%, si-IRF7: 3.27% ± 0.28%, positive: 0.47% ± 0.17%, si-mock: 1.10% ± 0.22%. F Immunofluorescence staining for S100A12 + inflammatory macrophages in the ankle synovium of different groups. Scale bar: 100 μm. The data are presented as the means ± SDs. Statistical significance: *** P < 0.001, **** P < 0.0001 (one-way ANOVA with Bonferroni post hoc correction)

    Journal: Arthritis Research & Therapy

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis

    doi: 10.1186/s13075-025-03708-3

    Figure Lengend Snippet: Local IRF7 knockdown alters the immune cell composition in CIA mice. A Schematic of intra-articular IRF7 siRNA treatment in collagen-induced arthritis (CIA) model mice. B – C Flow cytometry analysis of the CD86 and CD206 mean fluorescence intensities (MFIs) in F4/80 + macrophages from ankle joints ( n = 6). CD86: NC: 1241 ± 265.4, si-IRF7: 2469 ± 390.3, positive: 3489 ± 570.9, si-mock: 3689 ± 370.1. CD206: NC: 2225 ± 225.9, si-IRF7: 3395 ± 369.4, positive: 978.2 ± 147.9, si-mock: 2022 ± 170.6. D – E Frequencies of Foxp3 + Tregs among CD3 + CD4 + T cells ( n = 6). NC: 1.66% ± 0.15%, si-IRF7: 3.27% ± 0.28%, positive: 0.47% ± 0.17%, si-mock: 1.10% ± 0.22%. F Immunofluorescence staining for S100A12 + inflammatory macrophages in the ankle synovium of different groups. Scale bar: 100 μm. The data are presented as the means ± SDs. Statistical significance: *** P < 0.001, **** P < 0.0001 (one-way ANOVA with Bonferroni post hoc correction)

    Article Snippet: Double immunofluorescence staining was performed as follows: sections were incubated overnight at 4 °C with primary antibodies against CD68 (mouse monoclonal, 1:100 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 dilution; 22392-1-AP, Proteintech, China).

    Techniques: Knockdown, Flow Cytometry, Fluorescence, Immunofluorescence, Staining

    Local IRF7 inhibition attenuates joint inflammation and bone erosion in CIA mice. A Representative ankle joint images on day 42 postimmunization. B H&E staining and histological staining. C - E IHC staining for CD68, S100A12, and IRF7 in the ankle synovium. Scale bar: 100 μm. F Quantification of paw thickness at the ankle joint ( n = 6 per group). 42 Days after the first immunization: NC: 8.33 ± 0.02, si-IRF7: 10.39 ± 0.54, positive: 12.08 ± 0.80, si-mock: 12.65 ± 0.57, Statistical significance: **** P < 0.0001 (one-way ANOVA with Bonferroni post hoc correction). G H&E staining and histological scoring of synovial hyperplasia and inflammation ( n = 6). NC: 0.00 (0.00–0.00), si-IRF7: 1.50 (1.00–2.25), positive: 2.50 (1.75–3.00), and si-mock: 3.00 (2.75–3.00). Data are shown as medians with 25% − 75% percentiles. Statistical significance: * P < 0.05 (Kruskal‒Wallis test, followed by post hoc Dunn’s test with Bonferroni correction for multiple comparisons). H - J Semiquantitative analysis analysis of CD68, S100A12, and IRF7 expression via IHC staining via ImageJ ( n = 6). Statistical significance: **** P < 0.0001 (one-way ANOVA with Bonferroni post hoc correction)

    Journal: Arthritis Research & Therapy

    Article Title: IRF7 orchestrates proinflammatory macrophage polarization and joint destruction in rheumatoid arthritis

    doi: 10.1186/s13075-025-03708-3

    Figure Lengend Snippet: Local IRF7 inhibition attenuates joint inflammation and bone erosion in CIA mice. A Representative ankle joint images on day 42 postimmunization. B H&E staining and histological staining. C - E IHC staining for CD68, S100A12, and IRF7 in the ankle synovium. Scale bar: 100 μm. F Quantification of paw thickness at the ankle joint ( n = 6 per group). 42 Days after the first immunization: NC: 8.33 ± 0.02, si-IRF7: 10.39 ± 0.54, positive: 12.08 ± 0.80, si-mock: 12.65 ± 0.57, Statistical significance: **** P < 0.0001 (one-way ANOVA with Bonferroni post hoc correction). G H&E staining and histological scoring of synovial hyperplasia and inflammation ( n = 6). NC: 0.00 (0.00–0.00), si-IRF7: 1.50 (1.00–2.25), positive: 2.50 (1.75–3.00), and si-mock: 3.00 (2.75–3.00). Data are shown as medians with 25% − 75% percentiles. Statistical significance: * P < 0.05 (Kruskal‒Wallis test, followed by post hoc Dunn’s test with Bonferroni correction for multiple comparisons). H - J Semiquantitative analysis analysis of CD68, S100A12, and IRF7 expression via IHC staining via ImageJ ( n = 6). Statistical significance: **** P < 0.0001 (one-way ANOVA with Bonferroni post hoc correction)

    Article Snippet: Double immunofluorescence staining was performed as follows: sections were incubated overnight at 4 °C with primary antibodies against CD68 (mouse monoclonal, 1:100 dilution; 66231-2-Ig, Proteintech, China) with S100A12 (rabbit polyclonal, 1:100 dilution; 16630-1-AP, Proteintech, China) or CD68 (1:100 dilution; 66231-2-Ig) with IRF7 (rabbit polyclonal, 1:150 dilution; 22392-1-AP, Proteintech, China).

    Techniques: Inhibition, Staining, Immunohistochemistry, Expressing