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Promega rvprimer3
Rvprimer3, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rvprimer3/rvprimer3/pmc09747199-99-24-25
Average 90 stars, based on 1 article reviews
rvprimer3 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Sequencing:

Article Title: The SNP g.1311T>C associated with the absence of β-casein in goat milk influences CSN2 promoter activity.
Article Snippet: Quantitative individual differences in the amount of b-casein in goat milk are determined by at least nine alleles.. In particular, two alleles (CSN2 and CSN2) are associated with an undetectable amount of this protein in milk.. The CSN2 allele is characterized by a single nucleotide substitution at position 373 of the seventh exon (AJ011018:g.8915C>T), responsible for the formation of a premature stop codon at the 182 position.

Article Title: The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation.
Article Snippet: To construct the vector, the promoterless pGL4 luciferase vector (Promega Corporation) was modified. .. Briefly, the SV40 promoter and PCR product were ligated with the pGL4.10 basic vector, and the sequences were then confirmed by Sanger sequencing using RVprimer3 (Promega Corporation). ..

Article Title: Enhancer scanning to locate regulatory regions in genomic loci.
Article Snippet: .. Sanger sequencing, using the universal primers RVprimer3 and GLprimer2 (Promega), is recommended for verifying the variant after mutagenesis. ..

Article Title: Molecular Characterization of the Lipid Genome-Wide Association Study Signal on Chromosome 18q11.2 Implicates HNF4A-Mediated Regulation of the TMEM241 Gene
Article Snippet: To obtain constructs with the alternative allele, we utilized the GeneArt® Site-Directed Mutagenesis PLUS System (Invitrogen A14604) following the manufacturer’s protocol. .. All plasmid constructs were verified by sequencing using the RVprimer3 (Promega) and other appropriate custom designed sequencing primers. .. Reporter constructs were transiently transfected using Lipofectamine 2000 (invitrogen 11668-027) into human liver hepatocellular carcinoma cells (HepG2) (ATCC HB-8065).

Article Title: Genome-wide microarray analyses identifies the Protein C Receptor (PROCR/EPCR) as a novel Cn/NFAT-dependent gene in vascular smooth muscle cell phenotypic modulation
Article Snippet: .. Sequence fidelity was confirmed by sequencing with RVprimer3 and RVprimer4 (Promega) ( Supplemental Figure II ). .. Cells were transfected with the PROCR-WT-luc (or PROCR-MUT-luc) promoter construct 24 hrs prior to treatment using FuGENE6 (Roche).

Article Title: The EDN1/EDNRA/β-arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation
Article Snippet: To construct the vector, the promoterless pGL4 luciferase vector (Promega Corporation) was modified. .. Briefly, the SV40 promoter and PCR product were ligated with the pGL4.10 basic vector, and the sequences were then confirmed by Sanger sequencing using RVprimer3 (Promega Corporation). .. HCT116 cells were co-transfected with the pGL4.10-EDN1 promoter region or PGL4.10-EDNRA promoter region, and pRL-TK vectors with 50 nM siNC or siRNA for STAT3 (siSTAT3) using Lipofectamine 2000 ® (Thermo Fisher Scientific, Inc.).

Article Title: Genetic determinants of neuroglobin transcription
Article Snippet: .. We verified all constructs by Sanger sequencing using the commercial primer RVprimer3 (Promega) and pGLR 5’. ..

DNA Sequencing:

Article Title: The SNP g.1311T>C associated with the absence of β-casein in goat milk influences CSN2 promoter activity.
Article Snippet: Quantitative individual differences in the amount of b-casein in goat milk are determined by at least nine alleles.. In particular, two alleles (CSN2 and CSN2) are associated with an undetectable amount of this protein in milk.. The CSN2 allele is characterized by a single nucleotide substitution at position 373 of the seventh exon (AJ011018:g.8915C>T), responsible for the formation of a premature stop codon at the 182 position.

Article Title: Molecular and functional characterization of the BMPR2 gene in Pulmonary Arterial Hypertension
Article Snippet: PCR was performed with High Fidelity Phusion polymerase (Finnzymes, Espoo, Finland) and the resultant fragments were subcloned into the pGL3-Basic vector (Promega, Madison, Wisconsin, USA) at the NheI/XhoI restriction sites (New England Biolabs, Ipswich, Massachusetts, USA), using T4DNA Ligase (Invitrogen Corporation, Carlsbad, CA) to generate a construct with wild-type 5′UTR region driving firefly luciferase expression gene. .. The fragments were inserted with the same orientation, and their sequences were confirmed by DNA sequencing using RVprimer3 and GLprimer2 (Promega, Madison, Wisconsin, USA). .. The pGL3-Basic vector containing no promoter element was used as negative control and the pGL3-Promoter vector (Promega, Madison, Wisconsin, USA) fused with SV40 promoter element was used as positive control.

Polymerase Chain Reaction:

Article Title: The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation.
Article Snippet: To construct the vector, the promoterless pGL4 luciferase vector (Promega Corporation) was modified. .. Briefly, the SV40 promoter and PCR product were ligated with the pGL4.10 basic vector, and the sequences were then confirmed by Sanger sequencing using RVprimer3 (Promega Corporation). ..

Article Title: The EDN1/EDNRA/β-arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation
Article Snippet: To construct the vector, the promoterless pGL4 luciferase vector (Promega Corporation) was modified. .. Briefly, the SV40 promoter and PCR product were ligated with the pGL4.10 basic vector, and the sequences were then confirmed by Sanger sequencing using RVprimer3 (Promega Corporation). .. HCT116 cells were co-transfected with the pGL4.10-EDN1 promoter region or PGL4.10-EDNRA promoter region, and pRL-TK vectors with 50 nM siNC or siRNA for STAT3 (siSTAT3) using Lipofectamine 2000 ® (Thermo Fisher Scientific, Inc.).

Variant Assay:

Article Title: Enhancer scanning to locate regulatory regions in genomic loci.
Article Snippet: .. Sanger sequencing, using the universal primers RVprimer3 and GLprimer2 (Promega), is recommended for verifying the variant after mutagenesis. ..

Mutagenesis:

Article Title: Enhancer scanning to locate regulatory regions in genomic loci.
Article Snippet: .. Sanger sequencing, using the universal primers RVprimer3 and GLprimer2 (Promega), is recommended for verifying the variant after mutagenesis. ..

Plasmid Preparation:

Article Title: Molecular Characterization of the Lipid Genome-Wide Association Study Signal on Chromosome 18q11.2 Implicates HNF4A-Mediated Regulation of the TMEM241 Gene
Article Snippet: To obtain constructs with the alternative allele, we utilized the GeneArt® Site-Directed Mutagenesis PLUS System (Invitrogen A14604) following the manufacturer’s protocol. .. All plasmid constructs were verified by sequencing using the RVprimer3 (Promega) and other appropriate custom designed sequencing primers. .. Reporter constructs were transiently transfected using Lipofectamine 2000 (invitrogen 11668-027) into human liver hepatocellular carcinoma cells (HepG2) (ATCC HB-8065).

Construct:

Article Title: Molecular Characterization of the Lipid Genome-Wide Association Study Signal on Chromosome 18q11.2 Implicates HNF4A-Mediated Regulation of the TMEM241 Gene
Article Snippet: To obtain constructs with the alternative allele, we utilized the GeneArt® Site-Directed Mutagenesis PLUS System (Invitrogen A14604) following the manufacturer’s protocol. .. All plasmid constructs were verified by sequencing using the RVprimer3 (Promega) and other appropriate custom designed sequencing primers. .. Reporter constructs were transiently transfected using Lipofectamine 2000 (invitrogen 11668-027) into human liver hepatocellular carcinoma cells (HepG2) (ATCC HB-8065).

Article Title: Genetic determinants of neuroglobin transcription
Article Snippet: .. We verified all constructs by Sanger sequencing using the commercial primer RVprimer3 (Promega) and pGLR 5’. ..



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