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mifepristone ru 486  (Millipore)


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    Structured Review

    Millipore mifepristone ru 486
    IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and <t>mifepristone</t> treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).
    Mifepristone Ru 486, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ru-486/ru486/pmc11652171-51-7-10
    Average 90 stars, based on 1 article reviews
    mifepristone ru 486 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Insulin-like growth factor binding protein-6 modulates proliferative antagonism in response to progesterone in breast cancer"

    Article Title: Insulin-like growth factor binding protein-6 modulates proliferative antagonism in response to progesterone in breast cancer

    Journal: Frontiers in Endocrinology

    doi: 10.3389/fendo.2024.1450648

    IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and mifepristone treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).
    Figure Legend Snippet: IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and mifepristone treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).

    Techniques Used: Expressing, Western Blot, Cell Counting

    Related Articles

    Activity Assay:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Expressing:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Dominant Negative Mutation:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Comparison:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Blocking Assay:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Microscopy:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Sequencing:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Staining:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Migration:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Control:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Incubation:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Concentration Assay:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Solvent:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.

    Imaging:

    Article Title: Host-microbiome interactions in distinct subsets of preterm labor and birth.
    Article Snippet: Dams were injected subcutaneously (s.c.) on 16.5 dpc with 150 mg/100 mL of RU-486 (Sigma Aldrich) dissolved in DMSO (Sigma-Aldrich) and diluted 1:13 in PBS, or 100 mL of DMSO diluted 1:13 in PBS as a control.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To overexpress caMYPT1, we first incubated 32 hpf TgBAC(cxcr4b:sfGFP-AHPH); Tg(prim:mem-mCherry); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP) embryos with RU-486 (final concentration 5 mM,Millipore Sigma, catalog # M8046) for 1 hour before we imaged the embryos in Ringer’s solution supplemented with Rockout, Latrunculin A or 1% DMSO solvent control as described above.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation.
    Article Snippet: For geneSwitch (inducible GAL4-UAS)41 experiments, food was prepared with either 100 μl of RU-486 (4 mg ml−1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table 1 in the Supplementary Information for genotypes and stock information.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To assess the migration distance of the primordium, embryos of different genetic backgrounds [(1) cldnB:lyn2GFP, (2) TgBAC(cxcr4b:LexPR-LexOP:C3-T2A-H2A-Cerulean); cldnB:lyn2GFP, (3) TgBAC(cxcr4b:LexPR-LexOP:C3D12-T2A-H2A-Cerulean); cldnB:lyn2GFP, (4) TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2A-TagBFP); cldnB:lyn2GFP, (5) TgBAC(cxcr4b:LexPRLexOP:caROCK-T2A-H2A-TagBFP); cldnB:lyn2GFP, (6) TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 mM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf.

    Article Title: Senescent glia link mitochondrial dysfunction and lipid accumulation
    Article Snippet: For geneSwitch (inducible GAL4- UAS ) experiments, food was prepared with either 100 μl of RU-486 (4 mg ml −1 in 100% EtOH; Sigma-Aldrich, M8046-1G) or vehicle (100% EtOH), pipetted onto food vials and allowed to dry for 24 h. See Supplementary Table in the Supplementary for genotypes and stock information.

    Article Title: Elevated glucocorticoid alters the developmental dynamics of hypothalamic neurogenesis in zebrafish
    Article Snippet: Larvae were incubated from 6 hpf until 120 hpf with 50 μM dexamethasone (Sigma-Aldrich D2915) or from 48 hpf until 120 hpf with 2 μM RU-486 (Sigma-Aldrich, M8046) or with 10 μM Spironolactone (Sigma-Aldrich S3378).

    Article Title: Adipocyte Glucocorticoid Receptor Activation With High Glucocorticoid Doses Impairs Healthy Adipose Tissue Expansion by Repressing Angiogenesis.
    Article Snippet: Mature adipocytes were treated with indicated dexamethasone (Dex) (Sigma-Aldrich) concentrations, with or without 100 mmol/L of RU-486 (Sigma-Aldrich), under normoxic (21% O2, 5% CO2, and 74% N2) or hypoxic (1% O2, 5% CO2, and 94% N2) conditions for 16 h.

    Article Title: Pulses of RhoA signaling stimulate actin polymerization and flow in protrusions to drive collective cell migration.
    Article Snippet: To over express caROCK, we first incubated 24 hpf TgBAC(cxcr4b:sfGFP-AHPH); TgBAC(cxcr4b:Myl12.1-mScarlet); TgBAC(cxcr4b:LexPR-LexOP:caMYPT1-T2A-H2ATagBFP) embryos with RU-486 (final concentration 5 mM, Millipore Sigma, catalog # M8046) for 6 hours before imaging the embryos in Ringer’s solution.



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    IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and mifepristone treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).

    Journal: Frontiers in Endocrinology

    Article Title: Insulin-like growth factor binding protein-6 modulates proliferative antagonism in response to progesterone in breast cancer

    doi: 10.3389/fendo.2024.1450648

    Figure Lengend Snippet: IGFBP-6 is induced by Progesterone (P4) in T47D breast cancer cells. Asterisks indicate significance relative to the vehicle. (* means p< 0.05, ** means p<0.01, and *** means p<0.001) (A) IGFBP-6 transcriptional expression with various steroid hormone and mifepristone treatments (p<0.0001). V stands for ethanol, P for progesterone, E for estradiol, and R for mifepristone (RU486). (B) IGFBP-6 protein levels follow transcriptional levels (p<0.0001). (C) Representative intracellular IGFBP-6 immunoblot. (D) IGFBP-6 secretion. Units are in fg/mL/cell. IGFBP-6 concentrations are normalized by cell count (p<0.01). (E) Relative proliferation of T47D cells in response to steroid hormones (p<0.0001).

    Article Snippet: Cells were also treated with 25 nM Mifepristone (RU 486) (Millipore Sigma, St. Louis MO) either alone or in the presence of 50nM P4.

    Techniques: Expressing, Western Blot, Cell Counting