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primescript fast rt reagent kit with gdna eraser  (TaKaRa)


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    Structured Review

    TaKaRa primescript fast rt reagent kit with gdna eraser
    Primescript Fast Rt Reagent Kit With Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt/PrimeScript+FAST+RT+reagent+Kit+with+gDNA+Eraser/custom%40rr092a%4042720865
    Average 99 stars, based on 434 article reviews
    primescript fast rt reagent kit with gdna eraser - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Reverse Transcription:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    CCK-8 Assay:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Staining:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    RNA Extraction:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Extraction:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Quantitative RT-PCR:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Expressing:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Activity Assay:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Synthesized:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    SYBR Green Assay:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Generated:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Spectrophotometry:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).

    Concentration Assay:

    Article Title: Lysine Lactylation Modifies Cryab-Hspa1b Interactions and Prevents MAPK Signaling to Slow Diabetic Cardiomyopathy Progression.
    Article Snippet: Nanjing, Jiangsu, China). Total RNA was extracted from mouse myocardial tissues and cardiomyocytes using Trizol (R0016, Beyotime) and was reverse transcribed with PrimeScript FAST RT reagent (RR092A, Takara, Dalian, Liaoning, China). Quantitative PCR was performed using ABI Prism 7000 (Thermo Fisher Scientific). Primer sequences are listed in Supplementary Table 2. Cell Viability and Apoptosis Ass

    Article Title: CNN3 promotes vascular smooth muscle cells proliferation and migration to deteriorate atherosclerosis by β-catenin signaling pathway
    Article Snippet: Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).. Total mRNAs were cDNA using the Prime Script RT reagent kit (TakaRa Bio, Inc.) in 20 μL reaction volumes containing 1 μg of total RNA as template strands.. Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).Then, the expression levels of CNN3, β-catenin and aldehyde 3-phosphatedehydrogenase ( GAPDH ) as endogenous control were evaluated by qRT-PCR using SYBR Green Master Mix (TaKaRa Bio, Inc.) and a Light Cycler 480 Fast Real-Time PCR system (Roche Molecular Systems, Inc., Pleasanton, CA, USA).



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