Journal: Shock (Augusta, Ga.)
Article Title: Intestinal REG3G Protects Against Gastrointestinal Dysfunction in a Murine Model of Ethanol Intoxication and Burn Injury
doi: 10.1097/SHK.0000000000002737
Figure Lengend Snippet: (A) Quantitative RT-PCR of ileal tissue from C57Bl/6 WT mice exposed to sham or burn injury, with or without ethanol exposure. (B-E) Quantitative RT-PCR of ileal tissue from Reg3g-Tg or littermate control mice exposed to sham or burn ethanol. (B) REG3G (C) REG3B (D) Defensin, alpha, related sequence 1 (Defa-rs1) and (E) Beta defensin 3 (Defb3). Data shown as mean values ± SEM (dots represent individual animals). * p < 0.05 compared to all other groups, ^ p < 0.05 compared to sham groups, # p < 0.05 compared to both burn groups (one-way ANOVA with Tukey’s multiple comparison test). All experiments represent 8-10 mice per group.
Article Snippet: RT-PCR was performed on QuantStudio 3 Real-Time PCR System (Thermo Fisher) using TaqMan probes and reagents: cxcl1 (Mm04207460_m1), Defa-rs1 (Mm00655850_m1), defb3 (Mm04214158_s1), il6 (Mm00446190_m1), Reg3g (Mm00441127-m1), Reg3b (Mm00440616_g1), s100a8 (Mm00496696_g1), Tnfa (Mm00443258_m1), Il1b (Mm00434228_m1), Tjp1 (Mm01320638_m1), Cdh1 (Mm01247357_m1, Cldn1 (Mm01342184_m1), ccl2 (Mm00441242_m1), Saa1 (Mm00656927_g1).
Techniques: Expressing, Quantitative RT-PCR, Control, Sequencing, Comparison