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rri  (TaKaRa)


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  • 97

    Structured Review

    TaKaRa rri
    Rri, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rri/Recombinant+RNase+Inhibitor/pm42120382-265-5-6
    Average 97 stars, based on 1935 article reviews
    rri - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: .. For downstream genomics experiments, the cells were collected in collection media supplemented with 1:100 RRI (Recombinant RNase Inhibitor, Takara). .. For experiments involving bulk RNA, the sorted cells were washed with 1X PBS before lysing using TRIzol reagent (Invitrogen) and stored at -80°C for RNA extraction later.

    Reverse Transcription:

    Article Title: Sperm IGF2 protein levels: implications for early embryo development
    Article Snippet: .. Reverse transcription was performed by adding 2 μL of reverse transcription mix, which contained 25 mM Tris-HCL, pH 8.3 (Sigma, T6791), 30 mM NaCl (Ambion, AM9759), 1 mM GTP (Thermo Fisher Scientific, R0461), 2.5 mM MgCl2 (Ambion, AM9530G), 8 mM DTT (Thermo Fisher Scientific, R0861), 0.5 U/μL RRI (Takara, 2313B), 2 μM of various Smart-seq3 TSOs (5′-biotin AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT), and 2 U/μL of Maxima H-minus reverse transcriptase enzyme (Thermo Fisher Scientific, EP0751). ..

    other:

    Article Title: Introducing synthetic thermostable RNase inhibitors to single-cell RNA-seq
    Article Snippet: Fresh tubes of RRI (Cat. 2313B TaKaRa) were used in all comparisons in this study.

    Synthesized:

    Article Title: Enhanced Activities of OCT4 and SOX2 Promote Epigenetic Reprogramming by Shortening G1 Phase.
    Article Snippet: .. The first-strand cDNAs were synthesized with ReverTra Ace (Toyobo, Cat# 7601009), oligo-dT (Takara, Cat# 3806), and RRI (Takara, Cat# 2313A). qRT-PCR was performed on a CFX96 real-time system (Bio-Rad) with ChamQ SYBR qPCRMaster Mix (Vazyme, Cat# Q311). .. Dual-Luciferase Reporter Assay: 6 × OCT4 or 6 × SOX2 motif was cloned into pGL3-Basic vector (Promega, E1751).

    Article Title: Enhanced Activities of OCT4 and SOX2 Promote Epigenetic Reprogramming by Shortening G1 Phase
    Article Snippet: .. The first‐strand cDNAs were synthesized with ReverTra Ace (Toyobo, Cat# 7601009), oligo‐dT (Takara, Cat# 3806), and RRI (Takara, Cat# 2313A). qRT‐PCR was performed on a CFX96 real‐time system (Bio‐Rad) with ChamQ SYBR qPCR Master Mix (Vazyme, Cat# Q311). .. 6 × OCT4 or 6 × SOX2 motif was cloned into pGL3‐Basic vector (Promega, E1751).

    Article Title: α-Ketoglutarate inhibits the pluripotent-to-totipotent state transition in stem cells.
    Article Snippet: 1 School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China 2 Laboratory of Metabolism and Cell Fate, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 3 Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China 4 Joint School of Life Sciences, Guangzhou Medical University and Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 5 Guangzhou National Laboratory, Guangzhou, China 6 GMU-GIBH Joint School of Life Sciences, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou, China 7 Guangdong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 8 Guangdong Engineering Research Center of Early Clinical Trials of Biotechnology Drugs, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 9 BGI-Shenzhen, Shenzhen, China

    Quantitative RT-PCR:

    Article Title: Enhanced Activities of OCT4 and SOX2 Promote Epigenetic Reprogramming by Shortening G1 Phase.
    Article Snippet: .. The first-strand cDNAs were synthesized with ReverTra Ace (Toyobo, Cat# 7601009), oligo-dT (Takara, Cat# 3806), and RRI (Takara, Cat# 2313A). qRT-PCR was performed on a CFX96 real-time system (Bio-Rad) with ChamQ SYBR qPCRMaster Mix (Vazyme, Cat# Q311). .. Dual-Luciferase Reporter Assay: 6 × OCT4 or 6 × SOX2 motif was cloned into pGL3-Basic vector (Promega, E1751).

    Real-time Polymerase Chain Reaction:

    Article Title: Enhanced Activities of OCT4 and SOX2 Promote Epigenetic Reprogramming by Shortening G1 Phase
    Article Snippet: .. The first‐strand cDNAs were synthesized with ReverTra Ace (Toyobo, Cat# 7601009), oligo‐dT (Takara, Cat# 3806), and RRI (Takara, Cat# 2313A). qRT‐PCR was performed on a CFX96 real‐time system (Bio‐Rad) with ChamQ SYBR qPCR Master Mix (Vazyme, Cat# Q311). .. 6 × OCT4 or 6 × SOX2 motif was cloned into pGL3‐Basic vector (Promega, E1751).

    Lysis:

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: Cells sorted in collection media (1:1 FBS:PBS) were counted, and 55,000 cells were taken in LoBind 0.6-ml microcentrifuge tubes (Eppendorf) and washed with 0.5 ml of chilled PBS. .. The cell pellet was resuspended in 50 μl of chilled freshly made lysis buffer (10 mM tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl 2 , 0.1% IGEPAL-CA630, 0.1% Tween 20, 0.01% digitonin, 1× protease inhibitor, and 20 mM NaBu) supplemented with RRI (1 U/μl; Takara). ..

    Protease Inhibitor:

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: Cells sorted in collection media (1:1 FBS:PBS) were counted, and 55,000 cells were taken in LoBind 0.6-ml microcentrifuge tubes (Eppendorf) and washed with 0.5 ml of chilled PBS. .. The cell pellet was resuspended in 50 μl of chilled freshly made lysis buffer (10 mM tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl 2 , 0.1% IGEPAL-CA630, 0.1% Tween 20, 0.01% digitonin, 1× protease inhibitor, and 20 mM NaBu) supplemented with RRI (1 U/μl; Takara). ..

    Isolation:

    Article Title: α-Ketoglutarate inhibits the pluripotent-to-totipotent state transition in stem cells.
    Article Snippet: 1 School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China 2 Laboratory of Metabolism and Cell Fate, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 3 Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China 4 Joint School of Life Sciences, Guangzhou Medical University and Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 5 Guangzhou National Laboratory, Guangzhou, China 6 GMU-GIBH Joint School of Life Sciences, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou, China 7 Guangdong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 8 Guangdong Engineering Research Center of Early Clinical Trials of Biotechnology Drugs, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 9 BGI-Shenzhen, Shenzhen, China

    RNA Sequencing:

    Article Title: α-Ketoglutarate inhibits the pluripotent-to-totipotent state transition in stem cells.
    Article Snippet: 1 School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China 2 Laboratory of Metabolism and Cell Fate, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 3 Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China 4 Joint School of Life Sciences, Guangzhou Medical University and Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 5 Guangzhou National Laboratory, Guangzhou, China 6 GMU-GIBH Joint School of Life Sciences, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou, China 7 Guangdong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 8 Guangdong Engineering Research Center of Early Clinical Trials of Biotechnology Drugs, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 9 BGI-Shenzhen, Shenzhen, China

    Cell Culture:

    Article Title: α-Ketoglutarate inhibits the pluripotent-to-totipotent state transition in stem cells.
    Article Snippet: 1 School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China 2 Laboratory of Metabolism and Cell Fate, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 3 Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China 4 Joint School of Life Sciences, Guangzhou Medical University and Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China 5 Guangzhou National Laboratory, Guangzhou, China 6 GMU-GIBH Joint School of Life Sciences, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou, China 7 Guangdong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 8 Guangdong Engineering Research Center of Early Clinical Trials of Biotechnology Drugs, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China 9 BGI-Shenzhen, Shenzhen, China



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