Review



e. coli rosetta2 de3 plyss bl21 cells  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Millipore e. coli rosetta2 de3 plyss bl21 cells
    E. Coli Rosetta2 De3 Plyss Bl21 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/e++coli+bl21+de3/pmc12154512-99-6-13
    Average 90 stars, based on 1 article reviews
    e. coli rosetta2 de3 plyss bl21 cells - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Transformation Assay:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Expressing:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Solubility:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Purification:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Polyacrylamide Gel Electrophoresis:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Western Blot:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Construct:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Chromatography:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Filtration:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Generated:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Incubation:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Labeling:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Control:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Recombinant:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Methylation:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Sequencing:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Binding Assay:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Variant Assay:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Produced:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Fluorescence:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Lysis:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Protease Inhibitor:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Sonication:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].

    Centrifugation:

    Article Title: Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors
    Article Snippet: The expression vectors for periplasmic secretion of N-terminal 6xHis-tagged Avr2 (pJK153; pET28b-ProT7:OmpA-6xHis-TEV-Avr2) or Avr2Δ6 (pSM101; pET28b-ProT7:OmpA-HIS-TEV-Avr2Δ6) , and Cip1 (pJK159; pET28b-ProT7:OmpA-6xHis-TEV-Cip1), or C-terminal 6xHis-tagged EpiC1 (pJK254; pET28b-ProT7:OmpA-EPIC1-6xHis) and EpiC2B (pJK256; pET28b-ProT7:OmpA-EPIC2B-6xHis), were generated as described in and transformed into E. coli strain Rosetta2(DE3)pLysS (Novagen/Merck).

    Article Title: Preparation of conserved homology 1 domains complexed to ligands
    Article Snippet: The His6-SUMO-C1Bδ fusion protein is expressed in Escherichia coli BL21(DE3) Rosetta2 cells (Millipore Sigma).

    Article Title: Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.
    Article Snippet: For protein expression, the construct was transformed into E. coli Rosetta2(DE3)pLysS (EMD Millipore, 71403-M), and the transformants were obtained on the nutrient agar plates containing carbenicillin and chloramphenicol antibiotics.

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics.
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [31] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [32].

    Article Title: Identification of the Polo-like kinase substrate required for homologous synapsis in C. elegans
    Article Snippet: Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 50 μM IPTG for 16 h. Bacterial cell pellets were resuspended in lysis buffer (PBS, 300 mM NaCl, 50 mM imidazole, 0.5 mM EGTA, 2 mM MgCl2, and 1 mM DTT) containing the cOmplete Protease Inhibitor Cocktail (Sigma 11697498001) and lysed by two freeze/thaw cycles and sonication after lysozyme treatment (0.25 mg/ml) on ice for 30 min. After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min, the supernatant was loaded onto a HisTrap HP column (Cytiva) and washed with lysis buffer.

    Article Title: Discovery of CHD1 Antagonists for PTEN-Deficient Prostate Cancer.
    Article Snippet: CHD1 is a chromodomain-helicase DNA-binding protein that preferentially recognizes diand trimethylated lysine 4 on histone H3 (H3K4me2/3).. Genetic studies have established CHD1 as a synthetic lethal target in phosphatase and tensin homologue (PTEN)-deficient cancers.. Despite this attractive therapeutic link, no inhibitors or antagonists of CHD1 have been reported to date.

    Article Title: PARP enzyme de novo synthesis of protein-free poly(ADP-ribose).
    Article Snippet: Article PARP enzyme de novo syn thesis of protein-free poly(ADP-ribose)

    Article Title: B-Cell Epitope Mapping of the Treponema pallidum Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics
    Article Snippet: The plasmid was then transformed into E. coli Rosetta2 DE3 pLysS BL21 cells (Millipore Sigma, Burlington, MA, USA), and cells were grown in auto-inducing media prepared according to Studier et al. [ ] at room temperature for 36 h. Protein expression and solubility prior to purification were assessed by performing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and western blot with anti-His antibodies (Millipore Sigma, Burlington, MA, USA) as previously described [ ].



    Similar Products

    virus strains e coli rosetta2 de3 merck  (Merck & Co)
    86
    Merck & Co virus strains e coli rosetta2 de3 merck
    Virus Strains E Coli Rosetta2 De3 Merck, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/bl21+cells+coli+de3+e+plyss+rosetta/pm41533506-34-7-13
    Average 86 stars, based on 1 article reviews
    virus strains e coli rosetta2 de3 merck - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    e coli rosetta2 de3  (Merck & Co)
    86
    Merck & Co e coli rosetta2 de3
    E Coli Rosetta2 De3, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/bl21+cells+coli+de3+e+plyss+rosetta/pmc12828588-2-0-5
    Average 86 stars, based on 1 article reviews
    e coli rosetta2 de3 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    e coli rosetta2 de3 plyss sigma aldrich 71403 e coli  (Addgene inc)
    93
    Addgene inc e coli rosetta2 de3 plyss sigma aldrich 71403 e coli
    E Coli Rosetta2 De3 Plyss Sigma Aldrich 71403 E Coli, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/pCMV-DnpK+(Plasmid+%2371403)/pm41741634-344-177-174
    Average 93 stars, based on 1 article reviews
    e coli rosetta2 de3 plyss sigma aldrich 71403 e coli - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    bl 21 de3 rosetta2 plyss cells  (Merck & Co)
    86
    Merck & Co bl 21 de3 rosetta2 plyss cells
    Bl 21 De3 Rosetta2 Plyss Cells, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/bl21+cells+coli+de3+e+plyss+rosetta/pmc12847983-231-7-12
    Average 86 stars, based on 1 article reviews
    bl 21 de3 rosetta2 plyss cells - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    competent rosetta2 de3  (Merck & Co)
    86
    Merck & Co competent rosetta2 de3
    Competent Rosetta2 De3, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/bl21+cells+coli+de3+e+plyss+rosetta/pmc12312054-502-6-8
    Average 86 stars, based on 1 article reviews
    competent rosetta2 de3 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    e. coli rosetta2 de3 plyss bl21 cells  (Millipore)
    90
    Millipore e. coli rosetta2 de3 plyss bl21 cells
    E. Coli Rosetta2 De3 Plyss Bl21 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/e++coli+bl21+de3/pmc12154512-99-6-13
    Average 90 stars, based on 1 article reviews
    e. coli rosetta2 de3 plyss bl21 cells - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    e. coli rosetta2 (de3) cells  (Promega)
    90
    Promega e. coli rosetta2 (de3) cells
    E. Coli Rosetta2 (De3) Cells, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosetta2-(de3/rosetta+2++de3++plyss+cells/pm40259883-44-4-11
    Average 90 stars, based on 1 article reviews
    e. coli rosetta2 (de3) cells - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results