Synthesized:
Article Title: CD73 + mesenchymal stem cell (MSC) transplantation improves pressure ulcer healing by promoting angiogenesis through the HIF-1α/VEGF pathway in diabetic mice.
Article Snippet: Diabetic wound healing remains a major clinical challenge.. Although therapies based on mesenchymal stem cells (MSCs) have emerged as promising strategies to address this impairment, the specific contribution of CD73 expression to the therapeutic potential of MSCs has not been previously explored.. In this study, we explored the role of CD73 expression in the therapeutic potential of human adipose tissue-derived mesenchymal stem cells (hADMSCs) for treating diabetic pressure ulcers in a mouse model. Our findings demonstrated that CD73-positive hADMSCs (CD73hADMSCs) could promote enhanced wound healing in diabetic mice and mitigate the endothelial dysfunction caused by high glucose levels.
Article Title: The lectin receptor-like kinase SILecRLK45 acts as a negative regulator of ethylene to inhibit fruit ripening in tomato.
Article Snippet: Total RNA was extracted using the RNAprep Pure Plant Kit (DP441, Tiangen Biotech Co. LTD, China) following the manufacturer's protocol. .. First-strand cDNA was synthesized from 1.0 μg of total RNA following the manufacturer's instructions with commercial kits (RR092A, Takara Biomedical Technology Co., Ltd, Japan). qPCR reactions were performed in 96-well optical plates using the TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (RR820A, Takara Bio) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). ..
Real-time Polymerase Chain Reaction:Article Title: CD73 + mesenchymal stem cell (MSC) transplantation improves pressure ulcer healing by promoting angiogenesis through the HIF-1α/VEGF pathway in diabetic mice.
Article Snippet: Diabetic wound healing remains a major clinical challenge.. Although therapies based on mesenchymal stem cells (MSCs) have emerged as promising strategies to address this impairment, the specific contribution of CD73 expression to the therapeutic potential of MSCs has not been previously explored.. In this study, we explored the role of CD73 expression in the therapeutic potential of human adipose tissue-derived mesenchymal stem cells (hADMSCs) for treating diabetic pressure ulcers in a mouse model. Our findings demonstrated that CD73-positive hADMSCs (CD73hADMSCs) could promote enhanced wound healing in diabetic mice and mitigate the endothelial dysfunction caused by high glucose levels.
Article Title: The lectin receptor-like kinase SILecRLK45 acts as a negative regulator of ethylene to inhibit fruit ripening in tomato.
Article Snippet: Total RNA was extracted using the RNAprep Pure Plant Kit (DP441, Tiangen Biotech Co. LTD, China) following the manufacturer's protocol. .. First-strand cDNA was synthesized from 1.0 μg of total RNA following the manufacturer's instructions with commercial kits (RR092A, Takara Biomedical Technology Co., Ltd, Japan). qPCR reactions were performed in 96-well optical plates using the TB Green® Premix Ex TaqTM II (Tli RNaseH Plus) (RR820A, Takara Bio) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). ..
Ligation:Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A + ) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A+) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Reverse Transcription:Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A + ) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A+) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Polymerase Chain Reaction:Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A + ) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A+) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
cDNA Synthesis:Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A + ) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A+) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Library Amplification:Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A + ) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
Article Title: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib.
Article Snippet: .. Briefly, ribosomal RNA (rRNA) and poly(A+) RNA were depleted using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, no. A15026) and DynaBeads mRNA DIRECT Micropurification (Thermo Fisher Scientific, no. 61021) kits, respectively, followed by ligation of an adapter with unique molecular identifiers (UMI) to the 3′ end of RNA by T4 RNA ligase (NEB, no. M0351L), then reverse transcription by SMARTer PCR cDNA Synthesis kit (Takara/Clontech, no. 634925), and library amplification using Advantage 2 PCR kit (Takara/Clontech, no. 639137). .. Libraries were subjected to size selection using AMPure XP beads (Beckman Coulter, no. A63880), to select for fragments longer than 500 nt.
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