rn1  (MedChemExpress)

Journal: Plant Physiology

Article Title: A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception

doi: 10.1093/plphys/kiab269

Figure Lengend Snippet: RN1 effects differ from those of direct 2,4-D treatment and RN1 induces hypocotyl elongation through the SCF TIR1/AFB pathway. A, Chemical structure of RN1 and 2,4-D. B, Seedling phenotype (left) and hypocotyl length (right) in 5-d-old wild-type (WT) seedlings treated with RN1 or 2,4-D for 5 d. Representative images are shown. Scale bars represent 1 cm. C, Expression of HAT2 relative to DMSO treatment after 30 min of RN1, 2,4-D, and NAA treatment in cell cultures and after 30 min of RN1 treatment in 5-d-old WT seedlings. D, E, Hypocotyl length relative to the WT (D) and relative to DMSO treatment after 5 d of RN1 and 2,4-D treatments, relative to that in WT (E) in 5-d-old axr1-30 , cul1-6 , tir1-1 , afb1-3 , afb2-3 , afb3-4 , afb5-5 , tir1-1afb2-3 , and tir1-1afb5 mutants. For all graphs, means of at least three biological replicates are shown, horizontal dashed lines represent DMSO (B, C) or WT (D, E) and error bars represent se of the mean. Different letters (of the same color) indicate statistical differences according to the Tukey–Kramer honestly significant difference test or Wilcoxon rank-sum test at α = 0.05 (B–E); ns indicates no significant difference compared to DMSO according to the Student’s t test at α = 0.05 (C); letters of different colors (black and gray) indicate two different groups of samples (RN1 and 2,4-D treatments) that were not statistically compared (E). Concentrations in square brackets are in µM.

Article Snippet: RN1 (Chembridge ID 6389186; ) and 2,4-D stocks were dissolved in dimethyl sulfoxide (DMSO).

Techniques: Expressing

Journal: Plant Physiology

Article Title: A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception

doi: 10.1093/plphys/kiab269

Figure Lengend Snippet: Differentially expressed genes differ after direct RN1 and 2,4-D treatments. A, B, Venn diagrams of the numbers of genes significantly differentially regulated (A) or specifically up- and downregulated (B) by RN1 or 2,4-D treatment of 30 min in cell culture as determined by RNAseq. Genes with expression log2 fold change ≥0.5 after 2,4-D or RN1 treatment compared to DMSO treatment, with a P -value cutoff corrected for the false discovery rate set at 0.05, were considered as significantly differentially regulated. C, Expression of ZAT10 relative to DMSO treatment after 30 min of RN1, 2,4-D, and NAA treatment in cell cultures and after 30 min of RN1 treatment in 5-d-old WT seedlings. Means of at least three biological replicates are shown, horizontal dashed line represents WT DMSO and error bars represent se of the mean. Different letters indicate statistical differences according to the Tukey–Kramer honestly significant difference test at α = 0.05; asterisk indicates significant difference compared to DMSO according to the Student’s t test at α = 0.05. D, Activity of RN1 and 2,4-D on ZAT10 expression in ZAT10::GUS seedlings treated with RN1 or 2,4-D for 5 d. Whole-plant images are composed of multiple images. Scale bars represent 1 mm. Horizontal black line marks root-hypocotyl junctions. Concentrations in square brackets are in µM.

Article Snippet: RN1 (Chembridge ID 6389186; ) and 2,4-D stocks were dissolved in dimethyl sulfoxide (DMSO).

Techniques: Cell Culture, Expressing, Activity Assay

Journal: Plant Physiology

Article Title: A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception

doi: 10.1093/plphys/kiab269

Figure Lengend Snippet: RN1 requires the SCF TIR1/AFB complex to promote gene expression. A, Expression of ZAT10 , ATL31 , and WRKY33 relative to DMSO treatment after 30 min of RN1 treatment in 5-d-old wild-type (WT) seedlings. Concentrations in square brackets are in µM. B–D, Expression of ZAT10 , ATL31I , and WRKY33 relative to DMSO treatment after 30 min of 50-µM RN1 treatment in 5-d-old axr1-30 (B), tir1-1 (C), and tir1-1afb1-3afb2-3afb3-4 ( tir1afb123 ) (D) mutants, relative to that in WT. Means of at least three biological replicates are shown, horizontal gray lines represent DMSO (A) or WT (B–D) and error bars represent SE of the mean. Dots/asterisks indicate significant differences compared to DMSO (A) or WT (B–D) with P < 0.05 (*), P < 0.01 (**), or P < 0.1 (***) according to the Student’s t test or Wilcoxon rank-sum test.

Article Snippet: RN1 (Chembridge ID 6389186; ) and 2,4-D stocks were dissolved in dimethyl sulfoxide (DMSO).

Techniques: Gene Expression, Expressing

Journal: Plant Physiology

Article Title: A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception

doi: 10.1093/plphys/kiab269

Figure Lengend Snippet: RN1-induced hypocotyl elongation and gene expression likely occur downstream of IAA7 degradation. A, Hypocotyl length relative to the wild-type (WT; Ler and Col-0, respectively) in 5-d-old AUX/IAA gain-of-function mutants shy2-2 / iaa3 and axr2-1/iaa7 . B, Hypocotyl length relative to DMSO treatment in 5-d-old WT (Ler and Col-0) seedlings after 5 d of RN1 treatment. Concentrations in square brackets are in µM. C, Hypocotyl length relative to DMSO treatment after 5 d of 1-µM RN1 treatment in 5-d-old shy2-2 / iaa3 and axr2-1 / iaa7 mutants, relative to that in WT (Ler and Col-0, respectively). D–E, Expression of ZAT10 , ATL31 , and WRKY33 relative to DMSO treatment after 30 min of 50-µM RN1 treatment in 5-d-old shy2-2 / iaa3 (D) and axr2-1 / iaa7 (E) mutants, relative to that in WT (Ler and Col-0, respectively). Means of at least three biological replicates are shown, horizontal dashed lines represent WT (A, C–E) or DMSO (B) and error bars represent se of the mean. Dots/asterisks indicate significant differences compared to the relevant WT (A, C), DMSO for the same line (B) or WT for the same gene (D–E) with , * P < 0.05, ** P < 0.01, *** P < 0.001, or **** P < 0.1 according to the Student’s t test or Wilcoxon rank-sum test.

Article Snippet: RN1 (Chembridge ID 6389186; ) and 2,4-D stocks were dissolved in dimethyl sulfoxide (DMSO).

Techniques: Gene Expression, Expressing