Journal: Experimental Hematology & Oncology
Article Title: Tetrahydromagnolol targets TRIM38 to mediate PANoptosis in cancer cells and has the potential for synergistic cancer therapy
doi: 10.1186/s40164-025-00734-4
Figure Lengend Snippet: THM induced PANoptosis by assembling PANoptosome. A Immunoblot analysis was used to detect the activation markers of pyroptosis (GSDME, AIM2), apoptosis (PARP, Cleaved-CASP3, Cleaved-CASP8) and necroptosis (p-RIPK1, p-RIPK3, p-MLKL) in the cell lysates of HT29, HCT116, A549 and H1299 cells after treatment with THM. β-actin was used as a loading control. B Representative images of IHC staining for Ki67, Cleaved-PARP, Cleaved-CASP3, AIM2, p-MLKL and p-RIPK1 in tumor tissues, scale bar = 50 μm. C-D HCT116 (75 µM) and A549 (90 µM) cells were treated with THM for 24 h, then immunofluorescence confocal laser microscopy analysis was performed after staining with the specified PANoptosome complex antibodies. Representative images illustrated the pairwise co-localization of ASC with CASP8, RIPK3, and AIM2 respectively. Scale bar = 20 μm
Article Snippet: Primary antibodies were incubated with slides overnight at 4 °C, including Ki67 (CST, 9449, 1:1500), Cleaved-PARP (CST, 5625, 1:50), Cleaved-CASP3 (CST, 9664, 1:2000), AIM2 (Proteintech, 20590-1-AP, 1:100), p-MLKL (UpingBio, YP-Ab-10353, 1:200), p-RIPK1 (Proteintech, 28252-1-AP, 1:100) and TRIM38 (Proteintech, 13405-1-AP, 1:100).
Techniques: Western Blot, Activation Assay, Control, Immunohistochemistry, Immunofluorescence, Microscopy, Staining
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