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riboflavin phosphate sodium  (MedChemExpress)


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    Structured Review

    MedChemExpress riboflavin phosphate sodium
    Riboflavin Phosphate Sodium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/riboflavin/Riboflavin+phosphate+sodium/10__1016_slash_j__apsb__2026__05__019-61-32-45
    Average 94 stars, based on 2 article reviews
    riboflavin phosphate sodium - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Structure and transport mechanism of human riboflavin transporters
    Article Snippet: .. For purification of human RFVTs, cells were homogenized using a Dounce homogenizer, and the cell membrane was collected by ultracentrifugation at 100,000× g for 45 min. Then, the membrane was resuspended and solubilized in buffer A (20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM β-mercaptoethanol (β-ME), and a protease inhibitor cocktail including 2 μM aprotinin, 2 μM leupeptin, 0.8 μM pepstatin and 1 mM phenylmethylsulfonic acid acyl fluoride (PMSF)) supplemented with 1% (w/v) n-dodecyl-β- d -maltoside (DDM, Anatrace), 0.15% (w/v) cholesterol hemisuccinate (CHS, Anatrace) and 10 μM riboflavin (MCE). ..

    Membrane:

    Article Title: Structure and transport mechanism of human riboflavin transporters
    Article Snippet: .. For purification of human RFVTs, cells were homogenized using a Dounce homogenizer, and the cell membrane was collected by ultracentrifugation at 100,000× g for 45 min. Then, the membrane was resuspended and solubilized in buffer A (20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM β-mercaptoethanol (β-ME), and a protease inhibitor cocktail including 2 μM aprotinin, 2 μM leupeptin, 0.8 μM pepstatin and 1 mM phenylmethylsulfonic acid acyl fluoride (PMSF)) supplemented with 1% (w/v) n-dodecyl-β- d -maltoside (DDM, Anatrace), 0.15% (w/v) cholesterol hemisuccinate (CHS, Anatrace) and 10 μM riboflavin (MCE). ..

    Protease Inhibitor:

    Article Title: Structure and transport mechanism of human riboflavin transporters
    Article Snippet: .. For purification of human RFVTs, cells were homogenized using a Dounce homogenizer, and the cell membrane was collected by ultracentrifugation at 100,000× g for 45 min. Then, the membrane was resuspended and solubilized in buffer A (20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM β-mercaptoethanol (β-ME), and a protease inhibitor cocktail including 2 μM aprotinin, 2 μM leupeptin, 0.8 μM pepstatin and 1 mM phenylmethylsulfonic acid acyl fluoride (PMSF)) supplemented with 1% (w/v) n-dodecyl-β- d -maltoside (DDM, Anatrace), 0.15% (w/v) cholesterol hemisuccinate (CHS, Anatrace) and 10 μM riboflavin (MCE). ..

    other:

    Article Title: Non-canonical NOTCH1 signaling regulates ferroptosis vulnerability in dormant lung cancer cells with stable resistance.
    Article Snippet: AR TI CL E IN P RE SS ARTICLE IN PRESS Huang H et al., NOTCH1 signaling inhibits ferroptosis vulnerability in SRCC Materials and Methods (2064 words) Chemicals The compounds cisplatin (#HY-17394), RO4929097 (#HY-11102), PF-03084014 (#HY15185B), Ferrostain (#HY100579), Z-VAD-FMK (#HY16658B), FSEN1 (#HY-153629), BAI1 (#335165-68-9), PEG300 (#HY-Y0873) and riboflavin (#HY-B0456) were obtained from MedChemExpress (Monmouth Junction, NJ).

    Article Title: Non-canonical NOTCH1 signaling regulates ferroptosis vulnerability in dormant lung cancer cells with stable resistance
    Article Snippet: The chemicals and compounds cisplatin (#HY-17394), RO4929097 (#HY-11102), PF-03084014 (#HY-15185B), Ferrostain-1 (# HY100579 ), Z-VAD-FMK (#HY16658B), FSEN1 (#HY-153629), BAI1 (#HY-103269), PEG300 (#HY-Y0873) and riboflavin (#HY-B0456) were obtained from MedChemExpress (Monmouth Junction, NJ).

    Article Title: Non-canonical NOTCH1 signaling regulates ferroptosis vulnerability in dormant lung cancer cells with stable resistance.
    Article Snippet: The compounds cisplatin (#HY-17394), RO4929097 (#HY-11102), PF-03084014 (#HY15185B), Ferrostain (#HY100579), Z-VAD-FMK (#HY16658B), FSEN1 (#HY-153629), BAI1 (#335165-68-9), PEG300 (#HY-Y0873) and riboflavin (#HY-B0456) were obtained from MedChemExpress (Monmouth Junction, NJ).



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    Shanghai Yuanye Biochemicals riboflavin standard
    Large-fragment genome integration enabled by a PASTE-like two-step attB – attP recombination workflow using PE-STAR. ( A ) Schematic overview of the two-stage integration strategy. In phase I, PE-STAR–mediated prime editing installs a 46 bp attB site at the chromosomal xylB locus. In phase II, Bxb1 integrase catalyzes site-specific recombination between the genomic attB site and a donor plasmid–borne attP site, enabling targeted chromosomal integration of large DNA payloads, including a 3.2 kb GFP cassette or an 8.0 kb <t>riboflavin</t> biosynthetic pathway. ( B ) Transformation efficiencies (CFU per μg DNA) following attB–attP recombination for GFP and riboflavin donor constructs. ( C ) Phenotypic validation of large-fragment integration. Left, fluorescence microscopy confirming robust GFP expression in GFP integration strains. Right, colony morphology and liquid culture appearance of riboflavin-producing strains compared with non-integrated controls, showing characteristic yellow pigmentation indicative of riboflavin accumulation. ( D ) Growth profiles (OD 600 ) of the riboflavin-integrated strain and the control strain during 96 h fermentation. ( E ) Time-course quantification of riboflavin production by the PASTE-like/riboflavin strain during 96 h fermentation. Data are presented as mean ± s.d. ( n = 3 independent biological replicates).
    Riboflavin Standard, supplied by Shanghai Yuanye Biochemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Large-fragment genome integration enabled by a PASTE-like two-step attB – attP recombination workflow using PE-STAR. ( A ) Schematic overview of the two-stage integration strategy. In phase I, PE-STAR–mediated prime editing installs a 46 bp attB site at the chromosomal xylB locus. In phase II, Bxb1 integrase catalyzes site-specific recombination between the genomic attB site and a donor plasmid–borne attP site, enabling targeted chromosomal integration of large DNA payloads, including a 3.2 kb GFP cassette or an 8.0 kb riboflavin biosynthetic pathway. ( B ) Transformation efficiencies (CFU per μg DNA) following attB–attP recombination for GFP and riboflavin donor constructs. ( C ) Phenotypic validation of large-fragment integration. Left, fluorescence microscopy confirming robust GFP expression in GFP integration strains. Right, colony morphology and liquid culture appearance of riboflavin-producing strains compared with non-integrated controls, showing characteristic yellow pigmentation indicative of riboflavin accumulation. ( D ) Growth profiles (OD 600 ) of the riboflavin-integrated strain and the control strain during 96 h fermentation. ( E ) Time-course quantification of riboflavin production by the PASTE-like/riboflavin strain during 96 h fermentation. Data are presented as mean ± s.d. ( n = 3 independent biological replicates).

    Journal: Nucleic Acids Research

    Article Title: PE-STAR: prime editing with SOS-triggered and RecJ-augmented repair enables high-efficiency editing in Escherichia coli

    doi: 10.1093/nar/gkag285

    Figure Lengend Snippet: Large-fragment genome integration enabled by a PASTE-like two-step attB – attP recombination workflow using PE-STAR. ( A ) Schematic overview of the two-stage integration strategy. In phase I, PE-STAR–mediated prime editing installs a 46 bp attB site at the chromosomal xylB locus. In phase II, Bxb1 integrase catalyzes site-specific recombination between the genomic attB site and a donor plasmid–borne attP site, enabling targeted chromosomal integration of large DNA payloads, including a 3.2 kb GFP cassette or an 8.0 kb riboflavin biosynthetic pathway. ( B ) Transformation efficiencies (CFU per μg DNA) following attB–attP recombination for GFP and riboflavin donor constructs. ( C ) Phenotypic validation of large-fragment integration. Left, fluorescence microscopy confirming robust GFP expression in GFP integration strains. Right, colony morphology and liquid culture appearance of riboflavin-producing strains compared with non-integrated controls, showing characteristic yellow pigmentation indicative of riboflavin accumulation. ( D ) Growth profiles (OD 600 ) of the riboflavin-integrated strain and the control strain during 96 h fermentation. ( E ) Time-course quantification of riboflavin production by the PASTE-like/riboflavin strain during 96 h fermentation. Data are presented as mean ± s.d. ( n = 3 independent biological replicates).

    Article Snippet: The column temperature was maintained at 30°C, the injection volume was 15 μl, and the total run time was 40 min. Riboflavin was detected at 370 nm [ ], with a retention time of ~5 min. For calibration, 20 mg of riboflavin standard (Product B21290-20 mg; Shanghai Yuanye Bio-Technology Co., Ltd., China) was dissolved in 0.01 mol/l hydrochloric acid and diluted to a final volume of 200 ml, yielding a stock solution with a concentration of 0.1 mg/ml.

    Techniques: Plasmid Preparation, Transformation Assay, Construct, Biomarker Discovery, Fluorescence, Microscopy, Expressing, Control