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rhccl21 cat. #300-35  (PeproTech)

 
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    PeproTech rhccl21 cat. #300-35
    Rhccl21 Cat. #300 35, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rhccl21 cat. #300-35/product/PeproTech
    Average 90 stars, based on 1 article reviews
    rhccl21 cat. #300-35 - by Bioz Stars, 2026-03
    90/100 stars

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    Purified T cells (5 × 10 5 cells/well) were added to upper chambers of Transwell plates. rhCCL20 (200 ng/ml), rhCXCL16 (200 ng/ml), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), rhCCL21 (200 ng/ml), and PBS as a negative control were added to lower chambers. Plates were incubated for 90 minutes at 37°C. Migrated cells were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs and then counted using Neubauer chamber and flow cytometer. Migration index was calculated by the number of cells that migrated in the presence of each chemokine divided by the number of cells that migrated in the presence of PBS. The migration index of MAIT cells was normalized to the migration index of T cells. Values are expressed as the mean ± SEM. Data were obtained from 5 HCs. *, P < 0.05, **, P < 0.005 by paired t test.

    Journal: Oncotarget

    Article Title: Clinical relevance of circulating mucosal-associated invariant T cell levels and their anti-cancer activity in patients with mucosal-associated cancer

    doi: 10.18632/oncotarget.11187

    Figure Lengend Snippet: Purified T cells (5 × 10 5 cells/well) were added to upper chambers of Transwell plates. rhCCL20 (200 ng/ml), rhCXCL16 (200 ng/ml), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), rhCCL21 (200 ng/ml), and PBS as a negative control were added to lower chambers. Plates were incubated for 90 minutes at 37°C. Migrated cells were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs and then counted using Neubauer chamber and flow cytometer. Migration index was calculated by the number of cells that migrated in the presence of each chemokine divided by the number of cells that migrated in the presence of PBS. The migration index of MAIT cells was normalized to the migration index of T cells. Values are expressed as the mean ± SEM. Data were obtained from 5 HCs. *, P < 0.05, **, P < 0.005 by paired t test.

    Article Snippet: Recombinant human (rh) CCL20 (200 ng/ml; PeproTech, London, UA), rhCXCL16 (200 ng/ml; PeproTech), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), rhCCL21 (200 ng/ml; PeproTech) and PBS were added to lower chambers containing serum free RPMI1640 media.

    Techniques: Purification, Negative Control, Incubation, Staining, Flow Cytometry, Migration